FastQCFastQC Report
Fri 27 May 2016
SRR939254_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939254_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1200298
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31860.2654340838691725No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22820.19011945366900554No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17580.14646362819899725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46350.039.6498531
GGTATCA27300.036.181041
ATCAACG56550.032.2843553
TCAACGC56850.032.1139874
CAACGCA57000.032.029485
GTCTACG600.00395606431.658411
TATCAAC58900.031.2377992
AACGCAG59100.030.8759236
ACCCGTT804.551502E-429.622847
ACGCAGA65600.027.744327
CGCAGAG67200.026.9426788
GTACATG52500.025.4171811
TACATGG51350.025.118432
GCAGAGT72050.024.2070799
ACATGGG52200.023.1650753
GAGTACT32450.021.76297412-13
GGATACG1150.003728996320.646791
AGAGTAC62600.020.55574210-11
GTACTTT33850.020.44324914-15
AGTACTT34200.020.30290612-13
Produced by FastQC (version 0.11.5)