##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939254_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1200298 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.880496343408055 33.0 31.0 34.0 30.0 34.0 2 31.962616783498763 33.0 31.0 34.0 30.0 34.0 3 32.02075984463858 34.0 31.0 34.0 30.0 34.0 4 35.52536120196818 37.0 35.0 37.0 33.0 37.0 5 35.56514465574382 37.0 35.0 37.0 33.0 37.0 6 35.65562218715686 37.0 35.0 37.0 33.0 37.0 7 35.574749770473666 37.0 35.0 37.0 33.0 37.0 8 35.55145472207735 37.0 35.0 37.0 33.0 37.0 9 37.19252802220782 39.0 37.0 39.0 34.0 39.0 10-11 37.09341555180463 39.0 37.0 39.0 33.0 39.0 12-13 36.99292842277501 39.0 37.0 39.0 33.0 39.0 14-15 38.23735730626894 40.0 38.0 41.0 33.0 41.0 16-17 38.17345442548434 40.0 38.0 41.0 33.0 41.0 18-19 38.09574205738908 40.0 38.0 41.0 33.0 41.0 20-21 37.985972233561995 40.0 38.0 41.0 33.0 41.0 22-23 37.914638281493424 40.0 38.0 41.0 33.0 41.0 24-25 37.94414845313413 40.0 38.0 41.0 33.0 41.0 26-27 37.868802997255685 40.0 38.0 41.0 33.0 41.0 28-29 37.73154583278486 40.0 38.0 41.0 32.5 41.0 30-31 37.563707512634366 40.0 37.5 41.0 32.0 41.0 32-33 37.37337644484953 40.0 37.0 41.0 31.5 41.0 34-35 37.07985891836861 40.0 36.5 41.0 30.5 41.0 36-37 36.84329058283859 40.0 36.0 41.0 30.0 41.0 38-39 36.53156216206308 39.5 35.5 41.0 30.0 41.0 40-41 36.40749380570492 39.0 36.0 41.0 30.0 41.0 42-43 36.25101183206171 39.0 35.0 41.0 30.0 41.0 44-45 36.223770263717846 39.0 36.0 41.0 29.5 41.0 46-47 36.055337507852215 39.0 35.5 41.0 28.5 41.0 48-49 35.876919315036766 39.0 35.0 41.0 28.0 41.0 50-51 34.755867292955585 38.0 34.0 40.0 25.5 40.5 52-53 34.87705761402585 38.5 34.5 39.5 26.0 40.5 54-55 35.372005951855286 39.0 35.0 40.5 27.0 41.0 56-57 35.27890740466117 39.0 35.0 41.0 26.0 41.0 58-59 35.07730371957631 39.0 35.0 40.5 26.0 41.0 60-61 34.79284685969651 38.0 34.0 40.0 26.0 41.0 62-63 34.4358467647201 38.0 34.0 40.0 25.0 41.0 64-65 34.071888814277784 37.0 34.0 40.0 23.5 41.0 66-67 33.71113965032017 37.0 33.5 39.5 23.0 41.0 68-69 33.30466392512526 36.0 33.0 39.0 22.0 41.0 70-71 32.89648445636 36.0 33.0 39.0 21.5 40.5 72-73 32.46011448823542 35.0 32.0 38.5 20.0 40.0 74-75 32.00794927592981 35.0 32.0 37.5 19.0 39.5 76-77 31.548579186168766 35.0 31.5 37.0 18.0 39.0 78-79 31.111331519339366 35.0 31.0 36.0 14.5 39.0 80-81 30.646801877533747 35.0 31.0 36.0 9.5 37.5 82-83 30.18945628502255 34.0 30.5 35.5 7.0 37.0 84-85 29.763304612687847 34.0 30.0 35.0 2.0 37.0 86-87 29.41517273210486 34.0 30.0 35.0 2.0 36.0 88-89 28.999002747650998 34.0 29.0 35.0 2.0 36.0 90-91 28.600654170880897 34.0 29.0 35.0 2.0 35.5 92-93 28.178938063714178 34.0 29.0 35.0 2.0 35.0 94-95 27.697977085690386 34.0 27.5 35.0 2.0 35.0 96-97 27.12238044219019 34.0 26.5 35.0 2.0 35.0 98-99 26.370149746146375 33.0 25.0 35.0 2.0 35.0 100-101 23.81989972490165 31.0 12.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3901705886688056 1101 2 0.28488745172959895 1101 3 0.4412163771080415 1101 4 0.3821201899073259 1101 5 0.40455218665341164 1101 6 0.407821210357703 1101 7 0.4045690455461113 1101 8 0.42421832656949476 1101 9 0.379301201890236 1101 10-11 0.5624282784963768 1101 12-13 0.5792658060503797 1101 14-15 0.727139625594873 1101 16-17 0.7887270727437539 1101 18-19 0.7912588225685155 1101 20-21 0.7918380096944233 1101 22-23 0.8580368308476025 1101 24-25 0.8406969112792737 1101 26-27 0.7659747906441297 1101 28-29 0.7512871372772807 1101 30-31 0.7209592809347853 1101 32-33 0.7780611195278269 1101 34-35 0.7863056666931882 1101 36-37 0.7938417464633574 1101 38-39 0.8271931075423069 1101 40-41 0.7793677364698439 1101 42-43 0.6283131144246994 1101 44-45 0.9346002795582748 1101 46-47 0.9541738664417281 1101 48-49 0.8316786112924177 1101 50-51 0.6566686738955099 1101 52-53 0.7471817899768212 1101 54-55 0.7838906772508238 1101 56-57 0.765669704545715 1101 58-59 0.6648488660035596 1101 60-61 0.7340473517255575 1101 62-63 0.8042248086262447 1101 64-65 0.6525289155374523 1101 66-67 0.7387743618381961 1101 68-69 0.7276110097906141 1101 70-71 0.6484582346162071 1101 72-73 0.7119638637357468 1101 74-75 0.5381523426196111 1101 76-77 0.4784786945161521 1101 78-79 0.4899309249473518 1101 80-81 0.503568654591497 1101 82-83 0.4333149477971432 1101 84-85 0.310193132581837 1101 86-87 0.24557821657002776 1101 88-89 0.07957110958268387 1101 90-91 0.31324626869058037 1101 92-93 0.4561891016074249 1101 94-95 0.31905828230306454 1101 96-97 0.3501809987050528 1101 98-99 0.39490211530493724 1101 100-101 0.03263661962171582 1102 1 0.3133503483653364 1102 2 0.3704408348615651 1102 3 0.3494515336060928 1102 4 0.22517595434976556 1102 5 0.10419958559393194 1102 6 0.1805171880318781 1102 7 0.17057395856561186 1102 8 0.3113726187771775 1102 9 0.21652651563833558 1102 10-11 0.2939476381327566 1102 12-13 0.34409343259405745 1102 14-15 0.5206006305914457 1102 16-17 0.5635043993258648 1102 18-19 0.5033274711539306 1102 20-21 0.42905998125238654 1102 22-23 0.32230046280562874 1102 24-25 0.4987825047686911 1102 26-27 0.46404718248250276 1102 28-29 0.43205701077538805 1102 30-31 0.5511426000638053 1102 32-33 0.5573746336736463 1102 34-35 0.45058016076915663 1102 36-37 0.42117751959548855 1102 38-39 0.7070129634939022 1102 40-41 0.649598848917833 1102 42-43 0.6145867395312337 1102 44-45 0.5540275952782636 1102 46-47 0.6352052663180672 1102 48-49 0.6784617532853687 1102 50-51 0.5861651729513397 1102 52-53 0.7066184638291944 1102 54-55 0.6152069988363635 1102 56-57 0.5381074980317848 1102 58-59 0.5838191372222994 1102 60-61 0.5038765658095485 1102 62-63 0.5221439610468224 1102 64-65 0.5307328225577166 1102 66-67 0.48343604864156475 1102 68-69 0.2421094306057796 1102 70-71 0.273445283629421 1102 72-73 0.40702995554570975 1102 74-75 0.07479695083137017 1102 76-77 0.0684487819144266 1102 78-79 -0.10509673167603495 1102 80-81 0.06710201800454385 1102 82-83 -0.126602165887455 1102 84-85 -0.19149718008004868 1102 86-87 -0.1866430029951509 1102 88-89 -0.07059432882910954 1102 90-91 -0.09076185283145577 1102 92-93 -0.1966442400813868 1102 94-95 -0.09386178921980459 1102 96-97 -0.3014123904428523 1102 98-99 -0.14137401644419967 1102 100-101 -0.34191678022120087 1103 1 0.1718614242217349 1103 2 0.31024340845578635 1103 3 0.2438930401575945 1103 4 0.29000056214105996 1103 5 0.32900715360615607 1103 6 0.2081040027683443 1103 7 0.30173618901393695 1103 8 0.281127010927122 1103 9 0.27593071094946 1103 10-11 0.21368440292504687 1103 12-13 0.33568371404420105 1103 14-15 0.49077367248580117 1103 16-17 0.42507822857552213 1103 18-19 0.46309302729707014 1103 20-21 0.5632776203616174 1103 22-23 0.41872210920082864 1103 24-25 0.47746074673354855 1103 26-27 0.47806827655383444 1103 28-29 0.5411189415586222 1103 30-31 0.523834885156937 1103 32-33 0.38131405432115884 1103 34-35 0.41698535496387734 1103 36-37 0.4171819851927552 1103 38-39 0.6725221003409629 1103 40-41 0.6106130095618241 1103 42-43 0.43612443269769585 1103 44-45 0.6111696130231437 1103 46-47 0.6286904887165647 1103 48-49 0.6151648498245308 1103 50-51 0.5665327033585328 1103 52-53 0.6639602582942103 1103 54-55 0.75347158547482 1103 56-57 0.6848625952760443 1103 58-59 0.6604540746451733 1103 60-61 0.8073094937136531 1103 62-63 0.6240227435839927 1103 64-65 0.6040259067577338 1103 66-67 0.6805980440584491 1103 68-69 0.82818234910863 1103 70-71 0.6149826830065876 1103 72-73 0.47112009010120914 1103 74-75 0.460769980974149 1103 76-77 0.39990186376412495 1103 78-79 0.48882770571746903 1103 80-81 0.44395289055874443 1103 82-83 0.500013675231294 1103 84-85 0.21148625020374823 1103 86-87 0.22451511440305083 1103 88-89 0.06725895279730665 1103 90-91 0.0869219123721443 1103 92-93 0.20069109101914862 1103 94-95 0.054604630663853015 1103 96-97 -0.3982159629206734 1103 98-99 -0.34878191350043153 1103 100-101 -0.22943220415915277 1104 1 0.1245646349894578 1104 2 0.14072565022835093 1104 3 0.1515615365357732 1104 4 0.167572810681051 1104 5 0.10161223669511088 1104 6 0.032022467815842504 1104 7 0.2126944064237648 1104 8 0.2831729041020523 1104 9 0.34529369563502144 1104 10-11 0.2388100389377783 1104 12-13 0.25159531140740654 1104 14-15 0.3355192047313267 1104 16-17 0.39614591536901855 1104 18-19 0.11009342832972635 1104 20-21 0.10195220742330235 1104 22-23 0.09264339400921529 1104 24-25 0.15595161070827857 1104 26-27 0.1799944865445653 1104 28-29 0.2599417853814643 1104 30-31 0.2650184327920968 1104 32-33 0.31314163534229067 1104 34-35 0.3670708877107032 1104 36-37 0.36464514031720796 1104 38-39 0.39365714808891283 1104 40-41 0.3020669409351058 1104 42-43 0.10013739106548769 1104 44-45 0.13762523625295842 1104 46-47 0.3588628700986263 1104 48-49 0.35860890576858395 1104 50-51 0.44553519728037827 1104 52-53 0.671561080411152 1104 54-55 0.746566304375996 1104 56-57 0.615361755613911 1104 58-59 0.6492878215434317 1104 60-61 0.43768740433349507 1104 62-63 0.359607499330032 1104 64-65 0.4766620072164116 1104 66-67 0.48292653993533463 1104 68-69 0.3958409449930329 1104 70-71 0.30249364874336493 1104 72-73 0.23959265696087328 1104 74-75 0.3080094350684419 1104 76-77 0.5777473158031192 1104 78-79 0.4141682701709364 1104 80-81 0.4271941117031943 1104 82-83 0.12533794773298723 1104 84-85 0.18571520225528104 1104 86-87 0.23921233474543158 1104 88-89 0.2831758763755232 1104 90-91 0.2184637576236632 1104 92-93 0.2551626051680813 1104 94-95 0.11343580844664558 1104 96-97 0.19783449200074799 1104 98-99 0.015216319449415039 1104 100-101 -0.2410762500773913 1105 1 0.25760218055964046 1105 2 0.23335560512984088 1105 3 0.33702685450761294 1105 4 0.2933297778218247 1105 5 0.30496099846423874 1105 6 0.2813841809504041 1105 7 0.3563021042090142 1105 8 0.35694562833169385 1105 9 0.3524441822241542 1105 10-11 0.3335104321042266 1105 12-13 0.2461025448180365 1105 14-15 0.30557639949478954 1105 16-17 0.4462689320390396 1105 18-19 0.3415450706821517 1105 20-21 0.48053619958432137 1105 22-23 0.4489774972427938 1105 24-25 0.44133887357596535 1105 26-27 0.411321362453684 1105 28-29 0.25423904599726654 1105 30-31 0.38881565384780714 1105 32-33 0.41627783769519766 1105 34-35 0.39547997861979667 1105 36-37 0.5703937541924518 1105 38-39 0.684187222887914 1105 40-41 0.5331005396253587 1105 42-43 0.3988503186948691 1105 44-45 0.6094523647380541 1105 46-47 0.596751776231649 1105 48-49 0.7535460508689127 1105 50-51 0.8162250492039149 1105 52-53 0.6661033092875925 1105 54-55 0.8335353622980577 1105 56-57 0.6619452852261603 1105 58-59 0.8758728118359969 1105 60-61 0.8361552876251466 1105 62-63 0.7487677298740749 1105 64-65 0.6113850409276935 1105 66-67 0.8090173333890505 1105 68-69 0.8258743580551666 1105 70-71 0.591932017163451 1105 72-73 0.9158663397619691 1105 74-75 0.981626125166855 1105 76-77 0.7338575241326559 1105 78-79 0.7882793092623928 1105 80-81 0.7949979788543189 1105 82-83 0.6762924871013638 1105 84-85 0.6395010269614758 1105 86-87 0.5171562141906172 1105 88-89 0.5392624247270845 1105 90-91 0.5009144444477194 1105 92-93 0.7320975501186915 1105 94-95 0.5073428696187179 1105 96-97 0.49731731987783334 1105 98-99 0.3300587333453535 1105 100-101 0.2721037270386155 1106 1 0.1813676935226205 1106 2 0.39819655302796875 1106 3 0.25955389020286646 1106 4 0.25804635098158 1106 5 0.15780128985647934 1106 6 0.27479051291953027 1106 7 0.2580340149121625 1106 8 0.16192347878688196 1106 9 0.23916755533933554 1106 10-11 0.46410917751546066 1106 12-13 0.43831948688590927 1106 14-15 0.5081227689871426 1106 16-17 0.4113009592870398 1106 18-19 0.46342478555381916 1106 20-21 0.4728719451250498 1106 22-23 0.5215594174321581 1106 24-25 0.3290345830992223 1106 26-27 0.36166143551255203 1106 28-29 0.4112169807743058 1106 30-31 0.34398315319280215 1106 32-33 0.2531142924429801 1106 34-35 0.5217775564744684 1106 36-37 0.5102959860113003 1106 38-39 0.6624856379381612 1106 40-41 0.528276501270966 1106 42-43 0.49696331600560484 1106 44-45 0.5152294659333094 1106 46-47 0.49623522957305966 1106 48-49 0.8206146768141522 1106 50-51 0.6431254530453714 1106 52-53 0.4936628540700241 1106 54-55 0.4659658494675796 1106 56-57 0.5182672411318592 1106 58-59 0.5902313935423749 1106 60-61 0.40500144842089725 1106 62-63 0.4675212119150274 1106 64-65 0.4805653270799368 1106 66-67 0.385632398091019 1106 68-69 0.5397511005813911 1106 70-71 0.450205798185614 1106 72-73 0.5109527847223845 1106 74-75 0.5976853733170486 1106 76-77 0.6910600411400729 1106 78-79 0.7118746727648855 1106 80-81 0.7434189768034933 1106 82-83 0.8307983610221186 1106 84-85 0.7760783425751718 1106 86-87 0.7456639281347428 1106 88-89 0.5591138225124297 1106 90-91 0.7530537351202149 1106 92-93 0.8167506710814045 1106 94-95 0.5659774277270166 1106 96-97 0.8588672308881584 1106 98-99 0.8044113372343986 1106 100-101 0.7689973700740822 1107 1 -0.1466277319656193 1107 2 -0.050029357475811054 1107 3 -0.1734236477714468 1107 4 -0.22245919121535707 1107 5 -0.06121519011536947 1107 6 -0.11974059962518879 1107 7 -0.16676511710355157 1107 8 -0.08758388148524432 1107 9 -0.05728719804756821 1107 10-11 0.012401066554225793 1107 12-13 -0.11360855252527102 1107 14-15 -0.2385577776083636 1107 16-17 -0.15507637928548235 1107 18-19 -0.14553949806590794 1107 20-21 -0.11597181770411424 1107 22-23 -0.06669493715115493 1107 24-25 -0.1451062297563368 1107 26-27 -0.3589707854715627 1107 28-29 -0.2776663691758401 1107 30-31 -0.1618038974200644 1107 32-33 -0.17005736904315683 1107 34-35 -0.2710454451090172 1107 36-37 -0.17805220471064587 1107 38-39 -0.20566725632023264 1107 40-41 -0.37209004614560826 1107 42-43 -0.16664878885627843 1107 44-45 -0.2267548751610562 1107 46-47 -0.19884277171718878 1107 48-49 -0.2543266755991951 1107 50-51 -0.3703605595052082 1107 52-53 -0.25424489327617295 1107 54-55 -0.49802893089721323 1107 56-57 -0.6255058513553919 1107 58-59 -0.5997017482088438 1107 60-61 -0.5121896254164113 1107 62-63 -0.5965410131744164 1107 64-65 -0.730933998472814 1107 66-67 -0.7140362430444043 1107 68-69 -0.5362942926506804 1107 70-71 -0.7527896273026258 1107 72-73 -0.7349184251773302 1107 74-75 -0.6609768752314267 1107 76-77 -0.9032115558337139 1107 78-79 -0.7790979974597363 1107 80-81 -0.575629759341016 1107 82-83 -0.7170038206681042 1107 84-85 -0.8457429582282998 1107 86-87 -0.7884367111239534 1107 88-89 -0.7969488717577029 1107 90-91 -0.6464023085135899 1107 92-93 -0.6913734858595326 1107 94-95 -0.756552042685275 1107 96-97 -0.7505031872691355 1107 98-99 -0.7606918825181097 1107 100-101 -0.8195208986491593 1108 1 0.2464242568490782 1108 2 0.21648322598592173 1108 3 0.200447835422068 1108 4 0.3133252064334471 1108 5 0.40590516598803816 1108 6 0.14510845590183408 1108 7 0.31862551235487047 1108 8 0.36375435968350445 1108 9 0.40379537913671015 1108 10-11 0.39170112932886525 1108 12-13 0.31637775118984734 1108 14-15 0.44742350413562804 1108 16-17 0.5172770990002036 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68-69 -0.7998468693633782 2316 70-71 -0.8624998237754369 2316 72-73 -1.2200811713815511 2316 74-75 -0.9322847316909915 2316 76-77 -0.7304712384356904 2316 78-79 -0.7453609668727736 2316 80-81 -0.6290459579232746 2316 82-83 -0.6819514524471515 2316 84-85 -0.8312632064561214 2316 86-87 -0.36649111520548416 2316 88-89 -0.5108270635210523 2316 90-91 -0.5483192061564033 2316 92-93 -0.5786168570484271 2316 94-95 -0.5520326774813178 2316 96-97 -0.21125768210589868 2316 98-99 -0.1599738124158243 2316 100-101 0.10844949552288341 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3948.0 3 1125.0 4 1170.0 5 1236.0 6 1413.0 7 1679.0 8 2029.0 9 2496.0 10 2952.0 11 3866.0 12 4708.0 13 6535.0 14 5977.0 15 6341.0 16 7596.0 17 9720.0 18 8376.0 19 6516.0 20 6604.0 21 7074.0 22 7758.0 23 8449.0 24 9756.0 25 11019.0 26 12840.0 27 14854.0 28 18037.0 29 21395.0 30 26030.0 31 31749.0 32 39582.0 33 51762.0 34 70706.0 35 101092.0 36 156919.0 37 247177.0 38 241287.0 39 38504.0 40 21.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.28174279925643 18.77068320905654 14.552540886020427 23.395033105666606 2 14.788958534032288 19.85645593797829 41.50833865055237 23.846246877437057 3 19.08175224494392 24.574555494846955 29.43623024595326 26.907462014255863 4 12.675697995478775 16.33713994944903 37.381693207317376 33.60546884775482 5 13.986653952967657 37.35883748071881 33.94641059074486 14.708097975568677 6 31.99285075960679 36.00896322676164 17.86209702159444 14.136088992037132 7 28.986071499137545 32.29166232451814 20.741936317563482 17.980329858780824 8 26.023969818871546 35.7253684056944 19.793901244346248 18.456760531087813 9 26.6430115577106 17.15390021668102 20.170093350418814 36.032994875189566 10-11 25.016975717117234 26.598749903606272 27.477402183812938 20.906872195463556 12-13 25.872756960316746 23.738213972031595 27.9939606181279 22.395068449523762 14-15 22.68994678647144 25.27063201798739 26.206349775366043 25.833071420175123 16-17 22.432052315611063 28.519227771159215 26.649681656105223 22.39903825712449 18-19 22.305214551939383 27.246972861952834 28.81787065063198 21.629941935475802 20-21 23.209091890643517 26.403443059166744 28.69588381176065 21.691581238429094 22-23 22.706294650897522 26.32668614463055 28.561398944721272 22.405620259750656 24-25 22.430888502243366 26.523855208894904 28.802951292253564 22.242304996608166 26-27 22.40934689403482 26.709128290056583 28.592758258523077 22.288766557385525 28-29 22.191763802389293 26.83447472497784 28.802218355045206 22.17154311758766 30-31 22.42630597248267 26.61156308132045 28.653475269674324 22.308655676522555 32-33 22.28133994110369 26.680345774039598 28.55754062878316 22.48077365607356 34-35 22.730148099310757 26.849487692211977 28.28676375719379 22.13360045128347 36-37 22.74380516322797 26.539135582393254 28.403648507185643 22.313410747193135 38-39 22.439676390526436 26.744550050843486 28.517238511316172 22.298535047313905 40-41 22.60336340869097 26.631087731702902 28.406607803044643 22.35894105656148 42-43 22.545406212879005 26.594034559463392 28.20355492172669 22.65700430593092 44-45 22.53295214701563 26.851856109550237 28.112713879091928 22.502477864342204 46-47 22.619596557448528 26.94887216599228 27.78455249715413 22.646978779405057 48-49 22.708099463798565 26.895738609639082 27.805755019702527 22.590406906859826 50-51 22.677412638808413 27.1110876994071 27.59734043042418 22.61415923136031 52-53 22.770812743420844 27.13708659218344 27.4947030621067 22.597397602289014 54-55 22.583975785311587 26.976462075512476 27.639849565286816 22.79971257388912 56-57 22.568502525289464 27.23171019665287 27.59454947038923 22.605237807668438 58-59 22.682629225690526 26.95641636371234 27.71200534871088 22.648949061886256 60-61 22.712355168004905 26.997155885779307 27.80891261606296 22.481576330152834 62-63 22.54329896390123 26.97146339546805 27.88793514225668 22.59730249837404 64-65 22.408657104428855 26.763716119688656 27.92345136942746 22.904175406455032 66-67 22.397576317079004 27.07138592450994 27.665641468191254 22.86539629021981 68-69 22.375912044019806 26.97379727690386 27.825324099956973 22.82496657911936 70-71 22.471362754059644 26.888360528646444 27.775975071357905 22.86430164593601 72-73 22.53213447143268 26.842777729465045 27.598728000053512 23.02635979904876 74-75 22.41418814053678 27.140111985229186 27.461676500495035 22.984023373738996 76-77 22.509793528057084 26.967850139268545 27.451015638292226 23.071340694382144 78-79 22.559858096803627 26.955923158616695 27.54180882272424 22.942409921855443 80-81 22.495795467463772 27.315460308544143 27.395580476257347 22.793163747734734 82-83 22.637178809807253 27.116745361886547 27.358641203301186 22.887434625005014 84-85 22.542270074496994 26.997500603007218 27.34576078609568 23.114468536400107 86-87 22.510807491633653 26.915189510442154 27.510845111987432 23.063157885936757 88-89 22.54615872145233 26.773897934918757 27.51575692610758 23.164186417521336 90-91 22.487135333862444 26.957591135695154 27.54615013418727 23.009123396255134 92-93 22.551912157020162 26.833755655351784 27.512397869155443 23.101934318472615 94-95 22.414945136550237 26.745482263981796 27.579619112768412 23.259953486699555 96-97 22.44393354988275 26.70796103448622 27.585671944361746 23.26243347126929 98-99 22.527426580221753 26.710842436502208 27.304614622993757 23.45711636028228 100-101 22.720732515841107 26.295609804221563 27.704525636298726 23.2791320436386 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 898.0 1 505.5 2 147.5 3 171.5 4 146.0 5 156.5 6 257.0 7 554.5 8 901.0 9 1029.0 10 1002.5 11 946.5 12 1026.0 13 1186.0 14 1298.0 15 1409.0 16 1469.0 17 1577.0 18 1612.0 19 1693.5 20 1875.0 21 2079.0 22 2423.5 23 2868.0 24 3562.0 25 4556.0 26 5718.0 27 7173.0 28 8748.5 29 10478.0 30 12620.5 31 14924.0 32 17485.0 33 20009.0 34 22434.0 35 24912.0 36 27693.5 37 30355.5 38 32347.0 39 33927.5 40 35771.0 41 38244.0 42 40440.0 43 42266.0 44 43912.5 45 45641.0 46 46174.0 47 46319.0 48 48380.5 49 48792.5 50 46523.0 51 44317.5 52 42800.0 53 41703.5 54 40551.5 55 37726.0 56 33374.0 57 29716.0 58 26382.0 59 22783.5 60 19289.5 61 16168.5 62 13649.5 63 10822.0 64 8168.5 65 6255.5 66 4737.0 67 3852.0 68 3012.0 69 2122.5 70 1538.5 71 1179.5 72 956.0 73 798.5 74 618.0 75 502.0 76 362.0 77 221.5 78 136.5 79 85.5 80 64.5 81 52.5 82 41.0 83 27.0 84 19.0 85 14.5 86 10.5 87 8.0 88 6.0 89 4.5 90 3.5 91 5.0 92 5.5 93 5.0 94 4.5 95 3.0 96 2.0 97 1.5 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2810135483021716 2 0.11355513380843757 3 0.1243857775319129 4 0.1256354671923139 5 0.13255041664653278 6 0.06081823013951535 7 0.06789980488178769 8 0.07273193823533823 9 0.06989930833842929 10-11 0.06585864510313273 12-13 0.0697743393723892 14-15 0.0659003014251461 16-17 0.06739992901762729 18-19 0.07135727960889712 20-21 0.07493972330204667 22-23 0.08281276816257296 24-25 0.09118568888725967 26-27 0.09143562681933987 28-29 0.0858953359915621 30-31 0.08172970379022543 32-33 0.07514800491211349 34-35 0.23531656305350837 36-37 0.3584109946030069 38-39 0.3315426669043854 40-41 0.3168796415556804 42-43 0.3366663945120295 44-45 0.3505379497424806 46-47 0.3554533957400579 48-49 0.36536760037923915 50-51 0.35949405897535447 52-53 0.36024387277159503 54-55 0.3469555060493311 56-57 0.32496096802627344 58-59 0.31225578981219665 60-61 0.2719741264252711 62-63 0.3399155876290721 64-65 0.364784411871052 66-67 0.36924163832648227 68-69 0.3801972510159977 70-71 0.3803222199820378 72-73 0.3654092567012525 74-75 0.3687417624623219 76-77 0.35220420262301533 78-79 0.3323757933446528 80-81 0.33075119678613146 82-83 0.3434147186781949 84-85 0.3507045750305341 86-87 0.3446644083385959 88-89 0.3595773716193812 90-91 0.38165522228646553 92-93 0.37723965215304867 94-95 0.3758233372045942 96-97 0.3810720337782784 98-99 0.3723242061554714 100-101 0.34999641755630684 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1200298.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.9906911862329 #Duplication Level Percentage of deduplicated Percentage of total 1 82.25513014048778 55.925831518653034 2 11.071386314832916 15.055024158705798 3 3.0607572381587436 6.243090005270346 4 1.2736298504307908 3.463798953848316 5 0.6615606271844097 2.248998215193288 6 0.39504923499815325 1.6115802324070192 7 0.2546655128617438 1.2120418968536515 8 0.17762597605413247 0.9661530307639776 9 0.1329802643175113 0.8137278076567984 >10 0.6609921537191426 7.902121381653685 >50 0.038080182630855844 1.7794692962727874 >100 0.01716183530419359 1.9229557829276132 >500 6.129181372800911E-4 0.2529534577111083 >1k 3.677508823680547E-4 0.6022542620825266 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3186 0.2654340838691725 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2282 0.19011945366900554 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1758 0.14646362819899725 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 4.165632201336668E-5 0.0 18-19 0.0 0.0 0.0 8.331264402673336E-5 0.0 20-21 0.0 0.0 0.0 1.666252880534667E-4 0.0 22-23 0.0 0.0 0.0 3.749068981203001E-4 0.0 24-25 0.0 0.0 0.0 4.1656322013366683E-4 0.0 26-27 0.0 0.0 0.0 4.1656322013366683E-4 0.0 28-29 0.0 0.0 0.0 0.0012080333383876337 0.0 30-31 0.0 0.0 0.0 0.005498634505764401 0.0 32-33 0.0 0.0 0.0 0.013163397756223872 0.0 34-35 0.0 0.0 0.0 0.02482716791996654 0.0 36-37 0.0 0.0 0.0 0.045030484096449386 0.0 38-39 0.0 0.0 0.0 0.0778973221649957 0.0 40-41 0.0 0.0 0.0 0.1160128568072262 0.0 42-43 0.0 0.0 0.0 0.15675273973629883 0.0 44-45 0.0 0.0 0.0 0.20644873189824525 0.0 46-47 0.0 0.0 0.0 0.2651841459370923 0.0 48-49 0.0 0.0 0.0 0.3315843232263988 0.0 50-51 0.0 0.0 0.0 0.4041496361736835 0.0 52-53 0.0 0.0 0.0 0.4805056744241847 0.0 54-55 0.0 0.0 0.0 0.5606524379779021 0.0 56-57 0.0 0.0 0.0 0.6621272384024635 0.0 58-59 0.0 0.0 0.0 0.7728080859919787 0.0 60-61 0.0 0.0 0.0 0.9033589991818698 0.0 62-63 0.0 0.0 0.0 1.0381588572171245 0.0 64-65 0.0 0.0 0.0 1.1787072876902236 0.0 66-67 0.0 0.0 0.0 1.338709220543565 0.0 68-69 0.0 0.0 0.0 1.523538321316873 0.0 70-71 0.0 0.0 0.0 1.7400262268203397 0.0 72-73 0.0 0.0 0.0 1.9693859358259367 0.0 74-75 0.0 0.0 0.0 2.193913511477983 0.0 76-77 0.0 0.0 0.0 2.4448928516085173 0.0 78-79 0.0 0.0 0.0 2.7316549723485335 0.0 80-81 0.0 0.0 0.0 3.0547414058842053 0.0 82-83 0.0 0.0 0.0 3.3838680061118156 0.0 84-85 0.0 0.0 0.0 3.7199928684376715 0.0 86-87 0.0 0.0 0.0 4.08027839753128 0.0 88-89 0.0 0.0 0.0 4.485594410721337 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4635 0.0 39.649853 1 GGTATCA 2730 0.0 36.18104 1 ATCAACG 5655 0.0 32.284355 3 TCAACGC 5685 0.0 32.113987 4 CAACGCA 5700 0.0 32.02948 5 GTCTACG 60 0.003956064 31.65841 1 TATCAAC 5890 0.0 31.237799 2 AACGCAG 5910 0.0 30.875923 6 ACCCGTT 80 4.551502E-4 29.62284 7 ACGCAGA 6560 0.0 27.74432 7 CGCAGAG 6720 0.0 26.942678 8 GTACATG 5250 0.0 25.417181 1 TACATGG 5135 0.0 25.11843 2 GCAGAGT 7205 0.0 24.207079 9 ACATGGG 5220 0.0 23.165075 3 GAGTACT 3245 0.0 21.762974 12-13 GGATACG 115 0.0037289963 20.64679 1 AGAGTAC 6260 0.0 20.555742 10-11 GTACTTT 3385 0.0 20.443249 14-15 AGTACTT 3420 0.0 20.302906 12-13 >>END_MODULE