FastQCFastQC Report
Fri 27 May 2016
SRR939254_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939254_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1200298
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33970.2830130517588132No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25780.2147799963009186No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18650.15537808110985774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27300.037.481141
GTATCAA45150.036.893281
ATCAACG54050.030.3772963
TCAACGC55650.029.4186444
TATCAAC56600.029.260232
CAACGCA56100.029.0980765
AACGCAG57900.028.1934746
ACGCAGA67150.024.1684517
CGCAGAG67800.023.8667568
GTACATG49250.023.1922021
TACATGG49700.022.7242342
GCAGAGT72600.021.9628899
GTACTAG3301.8189894E-1221.632881
ACATGGG50750.021.5060423
GAGTACT36100.021.0329212-13
GTGTAGC6550.020.3448141
TACCTGG9250.020.0074422
TAAACCG1200.00479065419.7723145
CATGGGG25200.019.7723124
AGTACTT37600.019.75210212-13
Produced by FastQC (version 0.11.5)