##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939254_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1200298 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.20152495463626 33.0 31.0 34.0 30.0 34.0 2 32.41168609795234 34.0 31.0 34.0 31.0 34.0 3 32.50579772689782 34.0 31.0 34.0 30.0 34.0 4 36.06073991625413 37.0 35.0 37.0 35.0 37.0 5 35.99326167335112 37.0 35.0 37.0 35.0 37.0 6 36.025875240981826 37.0 35.0 37.0 35.0 37.0 7 35.976199243854445 37.0 35.0 37.0 35.0 37.0 8 35.94536939993235 37.0 35.0 37.0 35.0 37.0 9 37.65425419354194 39.0 37.0 39.0 35.0 39.0 10-11 37.55544039896759 39.0 37.0 39.0 35.0 39.0 12-13 37.47302753149634 39.0 37.0 39.0 34.0 39.0 14-15 38.74914812821483 40.0 38.0 41.0 34.0 41.0 16-17 38.6848828374287 40.0 38.0 41.0 34.0 41.0 18-19 38.79951603685085 40.0 38.0 41.0 34.0 41.0 20-21 38.805204624184995 40.0 38.0 41.0 34.0 41.0 22-23 38.74277137844102 40.0 38.0 41.0 34.0 41.0 24-25 38.67311950865535 40.0 38.0 41.0 34.0 41.0 26-27 38.501762062421165 40.0 38.0 41.0 34.0 41.0 28-29 38.34292109126234 40.0 38.0 41.0 34.0 41.0 30-31 38.155881706043 40.0 38.0 41.0 33.0 41.0 32-33 37.959941614499066 40.0 37.0 41.0 33.0 41.0 34-35 37.9193258674096 40.0 37.0 41.0 33.0 41.0 36-37 37.840911590288414 40.0 37.0 41.0 33.0 41.0 38-39 37.73981794521027 40.0 37.0 41.0 33.0 41.0 40-41 37.56553289266499 40.0 37.0 41.0 32.5 41.0 42-43 37.38806321430178 40.0 37.0 41.0 32.0 41.0 44-45 37.16713599456135 40.0 36.5 41.0 31.0 41.0 46-47 36.89789910505558 40.0 36.0 41.0 31.0 41.0 48-49 36.59687885841683 39.5 36.0 41.0 30.0 41.0 50-51 36.3511486314232 39.0 35.5 41.0 30.0 41.0 52-53 36.108544711396675 39.0 35.0 40.5 29.5 41.0 54-55 35.81932236827854 39.0 35.0 40.0 28.5 41.0 56-57 35.56796562187057 38.0 35.0 40.0 28.0 41.0 58-59 35.26546241016814 38.0 34.0 40.0 28.0 41.0 60-61 34.923983044210686 38.0 34.0 40.0 27.0 41.0 62-63 34.52439644155035 37.0 34.0 40.0 26.0 41.0 64-65 34.08449110137649 37.0 33.0 39.5 26.0 41.0 66-67 33.70001074733108 36.0 33.0 39.0 25.5 40.0 68-69 33.294376063277625 36.0 32.5 39.0 25.0 40.0 70-71 32.871551897945345 35.0 32.0 38.5 23.5 40.0 72-73 32.35686762787241 35.0 31.5 37.5 22.0 39.5 74-75 31.82308643353567 35.0 31.0 37.0 20.5 39.0 76-77 29.823450093226846 33.0 28.5 35.0 18.0 37.5 78-79 30.824257809310687 34.0 30.5 36.0 19.0 38.0 80-81 30.865259710505224 34.0 31.0 36.0 18.5 37.0 82-83 30.53210577706536 34.0 30.5 35.0 18.0 37.0 84-85 30.123643461873634 34.0 30.0 35.0 14.0 36.5 86-87 29.53926899819878 34.0 29.5 35.0 7.0 36.0 88-89 28.998109219543814 34.0 29.0 35.0 2.0 36.0 90-91 28.53343294748471 34.0 29.0 35.0 2.0 35.0 92-93 28.12802362413334 33.0 28.5 35.0 2.0 35.0 94-95 27.665912548383815 33.0 27.0 35.0 2.0 35.0 96-97 27.033176344541104 33.0 26.5 35.0 2.0 35.0 98-99 26.17321073600056 33.0 25.0 34.5 2.0 35.0 100-101 22.70518529565158 28.5 10.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.25092850196340777 1101 2 0.2713841461183861 1101 3 0.306195559356226 1101 4 0.11325404806787986 1101 5 0.23343359791501683 1101 6 0.19616864441922388 1101 7 0.2473516293803968 1101 8 0.2526289179416068 1101 9 0.3933176078891165 1101 10-11 0.2364489797777125 1101 12-13 0.32429608116534325 1101 14-15 0.33889040024448747 1101 16-17 0.4473824500570416 1101 18-19 0.4666641047549902 1101 20-21 0.43204360145654874 1101 22-23 0.3954633788778992 1101 24-25 0.48042341199916194 1101 26-27 0.550561379565984 1101 28-29 0.5540112111816171 1101 30-31 0.5239902101032712 1101 32-33 0.5207236074553023 1101 34-35 0.5841213565050793 1101 36-37 0.6780205138044266 1101 38-39 0.6218952638339701 1101 40-41 0.8263185849778125 1101 42-43 0.8470707109435978 1101 44-45 0.8253379743291802 1101 46-47 0.7365014754773966 1101 48-49 0.731043452656003 1101 50-51 0.6340553762264491 1101 52-53 0.5861904332083796 1101 54-55 0.6278882929686347 1101 56-57 0.6575154472042115 1101 58-59 0.6083475085319421 1101 60-61 0.7987710931886056 1101 62-63 0.746103733768976 1101 64-65 0.6280186588293049 1101 66-67 0.6172170124689131 1101 68-69 0.7755962344856542 1101 70-71 0.6731677210151261 1101 72-73 0.4734663756064421 1101 74-75 0.5189384409386832 1101 76-77 0.5625383095516945 1101 78-79 0.7916866882127458 1101 80-81 0.8057468414874407 1101 82-83 0.7995446427847384 1101 84-85 0.7814778020465312 1101 86-87 0.8440047946824691 1101 88-89 1.1182379153573194 1101 90-91 1.0926147937763098 1101 92-93 1.0753992106798833 1101 94-95 1.2158279090723632 1101 96-97 1.0419380526144906 1101 98-99 1.1426625935410257 1101 100-101 1.0293639562529862 1102 1 0.1619827958421851 1102 2 0.1442773130106545 1102 3 0.22733992525920144 1102 4 0.20118372838739162 1102 5 0.16440066063121606 1102 6 0.19180541519511962 1102 7 0.2548080555764827 1102 8 0.3013563790468723 1102 9 0.25270164224683356 1102 10-11 0.20278226297818946 1102 12-13 0.3002590496986244 1102 14-15 0.3327340860117758 1102 16-17 0.43094054567709605 1102 18-19 0.3453776957711838 1102 20-21 0.291403404935771 1102 22-23 0.22310329131251905 1102 24-25 0.3526564963393284 1102 26-27 0.38255950793950433 1102 28-29 0.36388487639442957 1102 30-31 0.28165385208786375 1102 32-33 0.3471695224634104 1102 34-35 0.5898607325181899 1102 36-37 0.5193901433226458 1102 38-39 0.4261064234068357 1102 40-41 0.5747469200657918 1102 42-43 0.4623971593652456 1102 44-45 0.5895681843858327 1102 46-47 0.5232881267699838 1102 48-49 0.2500162305173319 1102 50-51 0.24489827657904328 1102 52-53 0.22011282755339323 1102 54-55 0.38217632291465264 1102 56-57 0.30718874811829977 1102 58-59 0.2774798759723254 1102 60-61 0.1789402080095428 1102 62-63 0.36583685988652803 1102 64-65 0.2336711378988383 1102 66-67 0.3217105871083703 1102 68-69 0.3575542565775365 1102 70-71 0.24597733315395942 1102 72-73 0.3153657422614131 1102 74-75 0.30698342681793633 1102 76-77 0.5416395946238062 1102 78-79 0.39637327433262826 1102 80-81 0.5109614936490026 1102 82-83 0.5817633557073165 1102 84-85 0.5211704876634187 1102 86-87 0.5885954599454166 1102 88-89 0.7335860880148601 1102 90-91 0.7014440245712521 1102 92-93 0.8689638235707129 1102 94-95 0.7609549958523338 1102 96-97 0.671710282356635 1102 98-99 0.47360960311639744 1102 100-101 0.5204469929669422 1103 1 0.1694744228472942 1103 2 0.19245875727882122 1103 3 0.19472931358989598 1103 4 0.16009729557700325 1103 5 0.17084933564149907 1103 6 0.16675987785449564 1103 7 0.11304003160327625 1103 8 0.08966650302595269 1103 9 0.15291610843168968 1103 10-11 0.15534263667817072 1103 12-13 0.1237798206017402 1103 14-15 0.3097964868578842 1103 16-17 0.20730813484215105 1103 18-19 0.1520937190392928 1103 20-21 0.14463361822679843 1103 22-23 0.2551703927873419 1103 24-25 0.2602744527409939 1103 26-27 0.15807510815103853 1103 28-29 0.1717613673750975 1103 30-31 0.2680293735638557 1103 32-33 0.24724443873345336 1103 34-35 0.35773781107744185 1103 36-37 0.4974000146429418 1103 38-39 0.4564181338481106 1103 40-41 0.4580538123626354 1103 42-43 0.3850791618330831 1103 44-45 0.5770454157155243 1103 46-47 0.4599765338444328 1103 48-49 0.5239013001453969 1103 50-51 0.6718402238144918 1103 52-53 0.44710178107592924 1103 54-55 0.44312291202094656 1103 56-57 0.6065380048313926 1103 58-59 0.5827416422335645 1103 60-61 0.46757350021238153 1103 62-63 0.4090899887425081 1103 64-65 0.4574504433809494 1103 66-67 0.3353938234912377 1103 68-69 0.24722903182872358 1103 70-71 0.13044469316336205 1103 72-73 0.19798957444896814 1103 74-75 0.21933139085811604 1103 76-77 0.4311968541572675 1103 78-79 0.4849654360342548 1103 80-81 0.4349435137441908 1103 82-83 0.32908987312902127 1103 84-85 0.24000678826862298 1103 86-87 0.2610718099072251 1103 88-89 0.5530452219240232 1103 90-91 0.776962562675724 1103 92-93 0.6202785306627838 1103 94-95 0.5320564119167308 1103 96-97 0.3504884126815675 1103 98-99 0.4146020233526073 1103 100-101 0.6366039352079689 1104 1 0.05886460256005677 1104 2 0.06111116222155033 1104 3 0.11962341590334802 1104 4 -0.01594977563135558 1104 5 0.1284182004684311 1104 6 -0.0049572938626738505 1104 7 0.12214472619184136 1104 8 0.09169673086262975 1104 9 0.1584616381707633 1104 10-11 0.046636154987815814 1104 12-13 0.13753868718318074 1104 14-15 0.06867403404188366 1104 16-17 0.05830100398018345 1104 18-19 -0.022989933866931267 1104 20-21 0.160208097510953 1104 22-23 0.0983070947950111 1104 24-25 -0.09841793014493305 1104 26-27 0.1120597435872881 1104 28-29 0.1252556777242546 1104 30-31 0.06970619136971123 1104 32-33 0.07105481294706095 1104 34-35 0.14807449052702282 1104 36-37 0.2914916756700876 1104 38-39 0.31281397814718304 1104 40-41 0.41130687650279896 1104 42-43 0.3465173652067648 1104 44-45 0.4366510878211969 1104 46-47 0.3007665681828513 1104 48-49 0.20393833300501285 1104 50-51 0.27075743563492694 1104 52-53 0.2616194766420108 1104 54-55 0.3379238995638687 1104 56-57 0.20716900465593824 1104 58-59 0.21599570895537568 1104 60-61 0.22479678767760447 1104 62-63 0.18472004864676705 1104 64-65 0.1738388936403652 1104 66-67 0.22154754432237667 1104 68-69 0.1510830183440035 1104 70-71 0.2558848891680725 1104 72-73 0.27284326018975236 1104 74-75 0.2738295360820757 1104 76-77 0.31307080457960623 1104 78-79 0.5917388149850531 1104 80-81 0.5409508576547601 1104 82-83 0.6385210333666294 1104 84-85 0.5079702316352943 1104 86-87 0.4909966789449953 1104 88-89 0.6053690181994327 1104 90-91 0.5263672798223773 1104 92-93 0.43969477749838504 1104 94-95 0.3474576325598875 1104 96-97 0.4742430182910127 1104 98-99 0.5419753220968744 1104 100-101 0.5887275284605948 1105 1 0.13864817842718224 1105 2 0.20956573202077067 1105 3 0.2369586481210888 1105 4 0.14570188863127242 1105 5 0.10091708974277225 1105 6 0.10982631243529539 1105 7 0.10255639459306565 1105 8 0.1420612923238025 1105 9 0.010442990152114362 1105 10-11 0.08963683359554864 1105 12-13 0.0756921894685405 1105 14-15 0.1943279167127443 1105 16-17 0.11903829758301043 1105 18-19 0.10546130375531959 1105 20-21 0.21299951844859066 1105 22-23 0.1206775659252699 1105 24-25 0.164496918279049 1105 26-27 0.2803810871697294 1105 28-29 0.20541849793276157 1105 30-31 0.20441515081757444 1105 32-33 0.20265564249175583 1105 34-35 0.34786246365050744 1105 36-37 0.31744689973235296 1105 38-39 0.3852119891874892 1105 40-41 0.4239778978226454 1105 42-43 0.3206975583817382 1105 44-45 0.4553816501222556 1105 46-47 0.2776908623395258 1105 48-49 0.1199244900159755 1105 50-51 0.32162334518229585 1105 52-53 0.32664523634792886 1105 54-55 0.21076330993592052 1105 56-57 0.40622473569600714 1105 58-59 0.35413003981163627 1105 60-61 0.07891856885552073 1105 62-63 0.08901619853691045 1105 64-65 0.25811862609242553 1105 66-67 0.1475153971831773 1105 68-69 0.23576862212230054 1105 70-71 0.24200500273597214 1105 72-73 0.02992033237779168 1105 74-75 0.3030039626128982 1105 76-77 0.3160347683790796 1105 78-79 0.523116890782191 1105 80-81 0.4461641169802917 1105 82-83 0.39540981473322034 1105 84-85 0.2867292772024328 1105 86-87 0.37822721385829894 1105 88-89 0.7342067274875319 1105 90-91 0.8450816377012238 1105 92-93 0.7570465945238318 1105 94-95 0.734749029152848 1105 96-97 0.6518058275444218 1105 98-99 1.0509969249500664 1105 100-101 1.0304889871933334 1106 1 0.12531026762163577 1106 2 0.10192285917511867 1106 3 0.09571652694455679 1106 4 0.07001097641936127 1106 5 0.056240810384466045 1106 6 0.10034311507095595 1106 7 0.1539641178728246 1106 8 0.1796598058499299 1106 9 0.24256525082108737 1106 10-11 0.20414365613718388 1106 12-13 0.19476368536838606 1106 14-15 0.24696395346501987 1106 16-17 0.18554076249156282 1106 18-19 0.09475845096622493 1106 20-21 0.19686694039817354 1106 22-23 0.19968149893758635 1106 24-25 0.16976025844847697 1106 26-27 0.23278892789778638 1106 28-29 0.2638862945123037 1106 30-31 0.2920880482671393 1106 32-33 0.26681817588170276 1106 34-35 0.19862407070463206 1106 36-37 0.19752465653005657 1106 38-39 0.37184923354922006 1106 40-41 0.22328058618411717 1106 42-43 0.2554267948774438 1106 44-45 0.3807266921476611 1106 46-47 0.3756510262853112 1106 48-49 0.16745340789552188 1106 50-51 0.3292936226313188 1106 52-53 0.445196496507954 1106 54-55 0.3868356279738734 1106 56-57 0.33382651669407437 1106 58-59 0.22033760408446312 1106 60-61 0.27326237917952767 1106 62-63 0.3842763314642923 1106 64-65 0.45732487133049915 1106 66-67 0.46985978571420617 1106 68-69 0.6681496454884837 1106 70-71 0.5659409663089292 1106 72-73 0.3935316116306993 1106 74-75 0.4769881853912423 1106 76-77 0.5406429728957178 1106 78-79 0.797247622786248 1106 80-81 0.8414838022188462 1106 82-83 0.8175837526542651 1106 84-85 0.6323698372200184 1106 86-87 0.7296012836828183 1106 88-89 0.9274995260998367 1106 90-91 0.8678782774752456 1106 92-93 0.8148066898075292 1106 94-95 1.099183199380608 1106 96-97 1.1187475189806335 1106 98-99 1.1784614570691652 1106 100-101 0.9785211258666493 1107 1 0.038893048690198384 1107 2 -0.03165581656237748 1107 3 -0.03453683062977575 1107 4 0.028212841412617706 1107 5 -0.02777772225291386 1107 6 0.0426544164608984 1107 7 -0.0741691294440443 1107 8 -0.07689992671707557 1107 9 0.0022508997566390576 1107 10-11 0.07395481090816958 1107 12-13 -0.010379707980973762 1107 14-15 -0.03063999938231632 1107 16-17 -0.1012180786321224 1107 18-19 -0.015912477425075622 1107 20-21 -0.11171320152899256 1107 22-23 0.03946508934283344 1107 24-25 -0.02889084705852696 1107 26-27 0.028321447984581027 1107 28-29 0.11404464299626227 1107 30-31 0.03302846231910195 1107 32-33 -0.08961693056367181 1107 34-35 -0.12115776027302161 1107 36-37 -0.14611647888722246 1107 38-39 -0.1230044407220916 1107 40-41 -0.005282254704980005 1107 42-43 -0.15509458216789795 1107 44-45 0.012968202909497961 1107 46-47 -0.06177639732621287 1107 48-49 -0.13761227740153004 1107 50-51 -0.3550870588536341 1107 52-53 -0.4590202649723025 1107 54-55 -0.47044400371928674 1107 56-57 -0.31084095148960955 1107 58-59 -0.20895539925844986 1107 60-61 -0.375983999332 1107 62-63 -0.29149928286918225 1107 64-65 -0.39639045328389244 1107 66-67 -0.6435822411463903 1107 68-69 -0.38731777577712734 1107 70-71 -0.12015369089592554 1107 72-73 -0.2906760594736397 1107 74-75 -0.04053131493132156 1107 76-77 -0.2575052295557505 1107 78-79 0.16945453147195977 1107 80-81 0.049212087949886296 1107 82-83 0.03257994056113134 1107 84-85 -0.08659092754450981 1107 86-87 0.07530241558124118 1107 88-89 0.27922376514739966 1107 90-91 -0.07417497453343813 1107 92-93 0.0845152113018699 1107 94-95 0.09998667131774042 1107 96-97 0.0951257372669545 1107 98-99 -0.011131528679470648 1107 100-101 0.101273457108217 1108 1 0.05828185197730562 1108 2 0.059136285246673026 1108 3 0.13056617684610927 1108 4 0.07638381670223282 1108 5 0.14923534628557178 1108 6 0.08679354788817051 1108 7 0.1444834985306187 1108 8 0.15722379638970096 1108 9 0.2276147073238306 1108 10-11 0.24132341217507758 1108 12-13 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-0.4639145111177214 2316 78-79 -0.5869136649517976 2316 80-81 -0.6970479380502574 2316 82-83 -0.586324128707183 2316 84-85 -0.8256757714209826 2316 86-87 -0.7470153994252584 2316 88-89 -0.7395343598985917 2316 90-91 -1.3069517166659388 2316 92-93 -0.9570255427023966 2316 94-95 -0.6529794303771759 2316 96-97 -0.5927351374172432 2316 98-99 -0.6059891410810501 2316 100-101 0.043933321220656296 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 7.0 4 9.0 5 32.0 6 48.0 7 153.0 8 345.0 9 612.0 10 1070.0 11 1676.0 12 2377.0 13 3454.0 14 4149.0 15 5404.0 16 6822.0 17 9397.0 18 9273.0 19 6815.0 20 6577.0 21 7019.0 22 7599.0 23 8434.0 24 9822.0 25 11695.0 26 13995.0 27 16697.0 28 20302.0 29 24841.0 30 30040.0 31 36540.0 32 45435.0 33 57760.0 34 76887.0 35 108930.0 36 168165.0 37 266426.0 38 215905.0 39 15583.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.11191581543356 19.619896256356576 15.135059720682479 23.13312820752739 2 15.420670533484184 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23.00170457669679 26.467135661310774 28.848627590814946 21.682532171177492 22-23 22.54531791271834 26.350872866571468 28.796640500942267 22.307168719767926 24-25 22.373170973517386 26.542139153892748 28.881601803553618 22.203088069036248 26-27 22.333260572896794 26.74558396915402 28.712865100484386 22.208290357464804 28-29 22.096554355668342 26.89423793091382 28.93119042104544 22.078017292372394 30-31 22.380025626969303 26.60664268373354 28.748652417982868 22.264679271314293 32-33 22.224147669995283 26.71503243361232 28.69612379592401 22.364696100468386 34-35 22.649833098731484 26.812554441306702 28.43216118500942 22.105451274952394 36-37 22.679482014196655 26.54400175322887 28.516675881395397 22.259840351179076 38-39 22.37614712914126 26.688923613873904 28.665637137052276 22.26929211993256 40-41 22.555141697706006 26.65629244473331 28.55081702205668 22.237748835504004 42-43 22.490178001973774 26.61635448591719 28.359048339935487 22.534419172173553 44-45 22.520284698101875 26.895314378453854 28.15555253776382 22.42884838568045 46-47 22.556500599434973 27.016271806061333 27.904177049513578 22.523050544990113 48-49 22.706111315689938 26.899320002199456 27.89740547763972 22.49716320447089 50-51 22.68224224317628 27.152882034294816 27.632013050092556 22.532862672436345 52-53 22.746309666432836 27.127388365222636 27.545701150880863 22.580600817463665 54-55 22.492539352727405 27.075609556960025 27.63846978000463 22.79338131030794 56-57 22.56335510015013 27.253065488736965 27.58681594070806 22.59676347040485 58-59 22.623032767081288 26.98781127894092 27.730173541394166 22.658982412583626 60-61 22.619543537558922 27.072610087363387 27.81319098805773 22.494655387019964 62-63 22.504799923684445 27.06007225816987 27.79902514372694 22.63610267441875 64-65 22.377942810868635 26.864953536538426 27.77639386219089 22.980709790402052 66-67 22.373735522345285 27.138260665268128 27.60118737180267 22.88681644058392 68-69 22.371685603693763 27.02317098977417 27.760573659453435 22.844569747078634 70-71 22.459130982472686 26.98179951978592 27.619474497166536 22.939595000574858 72-73 22.48495790212097 26.9639706139642 27.486757455231952 23.06431402868288 74-75 22.326372284216088 27.200995086220253 27.320757012008684 23.151875617554975 76-77 22.415500649673827 27.011734211589918 27.438155727985407 23.13460941075085 78-79 22.39314736840351 27.013749918770174 27.496796628837174 23.096306083989145 80-81 22.300430564766337 27.315285382459486 27.44113059909155 22.94315345368263 82-83 22.519324367782 27.159338764206886 27.3108428073695 23.010494060641605 84-85 22.416724846663076 27.0066683440279 27.29397199695409 23.28263481235493 86-87 22.384757355154928 26.905059559303684 27.419922489089345 23.290260596452043 88-89 22.327584729306427 26.886293356476575 27.38895654329596 23.397165370921037 90-91 22.284656250992878 26.98579787260727 27.519276465738812 23.210269410661045 92-93 22.324789343979578 26.846624754852545 27.421856905535126 23.40672899563275 94-95 22.135586329394865 26.842084215753086 27.4093183526091 23.613011102242943 96-97 22.164162566296035 26.74952386823939 27.43926924813671 23.647044317327865 98-99 22.1307125397193 26.718948127881575 27.29509671764845 23.855242614750672 100-101 22.23294180474725 26.301106685811952 27.74945836254489 23.71649314689591 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 184.0 1 135.5 2 106.5 3 132.0 4 118.0 5 123.0 6 215.0 7 464.5 8 765.5 9 934.5 10 880.0 11 794.5 12 894.0 13 1001.0 14 1116.5 15 1216.5 16 1285.0 17 1346.5 18 1412.0 19 1537.0 20 1732.5 21 2089.0 22 2411.5 23 2903.0 24 3628.0 25 4557.0 26 5817.0 27 7307.5 28 8761.5 29 10539.5 30 12914.5 31 15220.0 32 17740.0 33 20091.5 34 22485.0 35 24893.0 36 27734.5 37 30535.0 38 32379.5 39 33936.5 40 35687.5 41 38451.0 42 40965.5 43 42513.0 44 44077.5 45 45825.5 46 46308.0 47 46575.0 48 48731.5 49 49062.0 50 46603.5 51 44668.0 52 43023.5 53 41844.5 54 40985.0 55 37828.0 56 33203.0 57 29714.0 58 26567.0 59 22762.0 60 19024.0 61 15991.0 62 13414.5 63 10634.0 64 8084.0 65 6064.0 66 4519.0 67 3791.0 68 2908.0 69 1952.0 70 1488.5 71 1104.5 72 894.5 73 793.0 74 635.5 75 521.0 76 365.5 77 185.0 78 98.5 79 65.0 80 44.5 81 26.5 82 17.0 83 16.0 84 9.5 85 3.0 86 3.5 87 1.0 88 1.5 89 1.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2756815390844607 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0024993793208020008 16-17 4.998758641604002E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 8.331264402673337E-5 26-27 9.997517283208005E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.003124224151002501 36-37 0.018870313872055105 38-39 0.006123479335964902 40-41 0.017329029957560537 42-43 0.004873789675563902 44-45 7.498137962406003E-4 46-47 8.331264402673337E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 6.66501152213867E-4 60-61 0.001999503456641601 62-63 0.0015412839144945674 64-65 0.0 66-67 0.0 68-69 3.332505761069335E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.23110927453015834 78-79 0.0 80-81 0.001166377016374267 82-83 0.0 84-85 0.0 86-87 0.1033909912371761 88-89 0.35562002102811135 90-91 0.35657811643441883 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 2.0828161006683342E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1200298.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.96140659643986 #Duplication Level Percentage of deduplicated Percentage of total 1 81.55902818147244 54.61307247670073 2 11.425336925847812 15.30113262786027 3 3.2368870445872187 6.502395284980598 4 1.350935893349005 3.6184227056106972 5 0.6892760794402251 2.3077447906298443 6 0.40326281449482365 1.6201827171967549 7 0.26075026487832137 1.2222143154652667 8 0.18147789156023503 0.9721611908023614 9 0.1385318975917899 0.834866164909819 >10 0.6950430318163747 8.229326932835654 >50 0.04079382145362512 1.8894792236159295 >100 0.017804604569667717 2.0257716852056524 >500 4.980279648317948E-4 0.20959628441610287 >1k 3.7352097362384617E-4 0.6536335997701856 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3397 0.2830130517588132 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2578 0.2147799963009186 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1865 0.15537808110985774 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 4.165632201336668E-5 0.0 18-19 0.0 0.0 0.0 8.331264402673336E-5 0.0 20-21 0.0 0.0 0.0 1.666252880534667E-4 0.0 22-23 0.0 0.0 0.0 2.9159425409356677E-4 0.0 24-25 0.0 0.0 0.0 3.332505761069334E-4 0.0 26-27 0.0 0.0 0.0 3.332505761069334E-4 0.0 28-29 0.0 0.0 0.0 0.0011247206943609004 0.0 30-31 0.0 0.0 0.0 0.005332009217710935 0.0 32-33 0.0 0.0 0.0 0.01291345982414367 0.0 34-35 0.0 0.0 0.0 0.024743855275939808 0.0 36-37 0.0 0.0 0.0 0.045030484096449386 0.0 38-39 0.0 0.0 0.0 0.07806394745304916 0.0 40-41 0.0 0.0 0.0 0.11667935795944007 0.0 42-43 0.0 0.0 0.0 0.15779414778663298 0.0 44-45 0.0 0.0 0.0 0.20774007788065962 0.0 46-47 0.0 0.0 0.0 0.2668920551396403 0.0 48-49 0.0 0.0 0.0 0.333542170361027 0.0 50-51 0.0 0.0 0.0 0.40602417066428503 0.0 52-53 0.0 0.0 0.0 0.4826718031688797 0.0 54-55 0.0 0.0 0.0 0.5632767862647443 0.0 56-57 0.0 0.0 0.0 0.6650848372654123 0.0 58-59 0.0 0.0 0.0 0.7760989354310346 0.0 60-61 0.0 0.0 0.0 0.9066915049429392 0.0 62-63 0.0 0.0 0.0 1.0414497066561803 0.0 64-65 0.0 0.0 0.0 1.1817065428751858 0.0 66-67 0.0 0.0 0.0 1.341541850440474 0.0 68-69 0.0 0.0 0.0 1.5254545121294878 0.0 70-71 0.0 0.0 0.0 1.7417341360228877 0.0 72-73 0.0 0.0 0.0 1.9707189381303643 0.0 74-75 0.0 0.0 0.0 2.1936635735459027 0.0 76-77 0.0 0.0 0.0 2.4448511952865037 0.0 78-79 0.0 0.0 0.0 2.7311134401623596 0.0 80-81 0.0 0.0 0.0 3.054324842664072 0.0 82-83 0.0 0.0 0.0 3.3842012566879225 0.0 84-85 0.0 0.0 0.0 3.721200901776059 0.0 86-87 0.0 0.0 0.0 4.08040336649732 0.0 88-89 0.0 0.0 0.0 4.485344472789257 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2730 0.0 37.48114 1 GTATCAA 4515 0.0 36.89328 1 ATCAACG 5405 0.0 30.377296 3 TCAACGC 5565 0.0 29.418644 4 TATCAAC 5660 0.0 29.26023 2 CAACGCA 5610 0.0 29.098076 5 AACGCAG 5790 0.0 28.193474 6 ACGCAGA 6715 0.0 24.168451 7 CGCAGAG 6780 0.0 23.866756 8 GTACATG 4925 0.0 23.192202 1 TACATGG 4970 0.0 22.724234 2 GCAGAGT 7260 0.0 21.962889 9 GTACTAG 330 1.8189894E-12 21.63288 1 ACATGGG 5075 0.0 21.506042 3 GAGTACT 3610 0.0 21.03292 12-13 GTGTAGC 655 0.0 20.344814 1 TACCTGG 925 0.0 20.007442 2 TAAACCG 120 0.004790654 19.772314 5 CATGGGG 2520 0.0 19.772312 4 AGTACTT 3760 0.0 19.752102 12-13 >>END_MODULE