FastQCFastQC Report
Fri 27 May 2016
SRR939253_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939253_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2797198
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT101640.3633636231686137No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT58410.20881610811962545No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT44630.15955252363257802No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC41360.1478622535837649No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35650.12744896857498111No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT31380.11218369239503247No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28510.10192342479867353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG91300.023.856991
TACATGG92600.022.8882312
ACATGGG94550.021.5636653
CATGGGG55400.020.027274
GAGTACT53150.018.40911712-13
TACATCG3251.0260919E-817.5070725
AGAGTAC109550.017.36480310-11
GTGTAGC16150.017.3361721
AGTACTT58150.017.07066712-13
GTACTTT56450.016.95856714-15
ATGGGGA36700.016.2787155
GTGTAAG11500.016.093131
GTATAGG7400.016.031791
GTATAGC9850.015.8983281
TAAGGTG9400.015.6368585
GAGTACA83100.015.304091
TATACAG16000.015.1135275
CCGTAGA2551.04796774E-414.8752892
GGACCGA2551.053419E-414.8662336
GTATCAA191300.014.759641
Produced by FastQC (version 0.11.5)