##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939253_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2797198 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.840093193259825 33.0 31.0 34.0 30.0 34.0 2 31.91416910780002 33.0 31.0 34.0 30.0 34.0 3 31.970144408797662 34.0 31.0 34.0 30.0 34.0 4 35.441493952162126 37.0 35.0 37.0 33.0 37.0 5 35.467845679855344 37.0 35.0 37.0 33.0 37.0 6 35.551894073998334 37.0 35.0 37.0 33.0 37.0 7 35.48687579499199 37.0 35.0 37.0 33.0 37.0 8 35.46945943762294 37.0 35.0 37.0 33.0 37.0 9 37.09064356545372 39.0 37.0 39.0 33.0 39.0 10-11 36.98573000552696 39.0 37.0 39.0 33.0 39.0 12-13 36.89190146711101 39.0 37.0 39.0 33.0 39.0 14-15 38.12882731218885 40.0 38.0 41.0 33.0 41.0 16-17 38.05183347764441 40.0 38.0 41.0 33.0 41.0 18-19 37.95309967331594 40.0 38.0 41.0 33.0 41.0 20-21 37.914029146309986 40.0 38.0 41.0 32.5 41.0 22-23 37.91962331590399 40.0 38.0 41.0 33.0 41.0 24-25 37.836837256425895 40.0 38.0 41.0 32.0 41.0 26-27 37.75357232487654 40.0 38.0 41.0 32.0 41.0 28-29 37.61524354014267 40.0 38.0 41.0 32.0 41.0 30-31 37.46415949103353 40.0 37.0 41.0 31.5 41.0 32-33 37.275521790019866 40.0 37.0 41.0 31.0 41.0 34-35 36.99693282348979 40.0 37.0 41.0 30.5 41.0 36-37 36.772053497821744 40.0 36.5 41.0 30.0 41.0 38-39 36.19096288500135 39.5 35.5 40.5 29.0 41.0 40-41 36.19175385510786 39.0 35.5 40.0 30.0 41.0 42-43 36.419964371488895 39.5 36.0 41.0 30.0 41.0 44-45 36.36666353257796 40.0 36.0 41.0 30.0 41.0 46-47 36.16008966830378 39.5 36.0 41.0 29.0 41.0 48-49 35.962606865870775 39.0 35.5 41.0 28.0 41.0 50-51 34.84102287360423 38.0 34.0 40.0 25.5 40.5 52-53 34.958029785521084 38.5 34.5 39.5 26.5 40.5 54-55 35.460785936497885 39.0 35.0 40.5 27.0 41.0 56-57 35.366682837611066 39.0 35.0 41.0 27.0 41.0 58-59 35.15449406870733 38.5 35.0 40.0 26.0 41.0 60-61 34.83123289806442 38.0 34.0 40.0 26.0 41.0 62-63 34.45947301549622 38.0 34.0 40.0 25.5 41.0 64-65 34.06101462964009 37.0 34.0 40.0 24.5 41.0 66-67 33.68148447124587 36.5 33.5 39.5 23.0 41.0 68-69 33.2649776669367 36.0 33.0 39.0 22.5 41.0 70-71 32.832330782447286 35.5 32.5 39.0 22.0 40.0 72-73 32.385067127890125 35.0 32.0 38.0 20.5 40.0 74-75 31.92210097390317 35.0 32.0 37.0 19.5 39.0 76-77 31.44982854270595 35.0 31.0 37.0 18.0 39.0 78-79 31.043838691433358 35.0 31.0 36.0 17.0 39.0 80-81 30.624635975000697 34.5 31.0 36.0 11.5 37.5 82-83 30.192462778823668 34.0 30.0 35.0 7.5 37.0 84-85 29.768021427156746 34.0 30.0 35.0 4.5 36.5 86-87 29.347597488629695 34.0 29.5 35.0 2.0 36.0 88-89 28.908348282817308 34.0 29.0 35.0 2.0 36.0 90-91 28.490662977737006 34.0 29.0 35.0 2.0 35.0 92-93 28.04311636144456 34.0 28.5 35.0 2.0 35.0 94-95 27.54999091948443 34.0 27.0 35.0 2.0 35.0 96-97 26.926698968038732 33.0 25.5 35.0 2.0 35.0 98-99 26.12122148664485 33.0 24.5 35.0 2.0 35.0 100-101 23.40265615805531 29.5 10.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4191261488002951 1101 2 0.43950540117621273 1101 3 0.44457454297379684 1101 4 0.40438125981240347 1101 5 0.30068023854662584 1101 6 0.4638080422368702 1101 7 0.3896648884196665 1101 8 0.3205072715878927 1101 9 0.5054571303716884 1101 10-11 0.5659948551099632 1101 12-13 0.5930262860822779 1101 14-15 0.6319258338991247 1101 16-17 0.6367000964576874 1101 18-19 0.709288700643377 1101 20-21 0.6538364883441474 1101 22-23 0.6799486835433939 1101 24-25 0.6527079442288368 1101 26-27 0.591480170706653 1101 28-29 0.6297366418179777 1101 30-31 0.7173510362863738 1101 32-33 0.754769168280724 1101 34-35 0.6398868089480274 1101 36-37 0.5347568462257968 1101 38-39 0.9344959085604856 1101 40-41 0.7973610006051359 1101 42-43 0.7719134350340866 1101 44-45 0.6007387175995049 1101 46-47 0.6992755422818675 1101 48-49 0.6607720525160659 1101 50-51 0.6760524210169336 1101 52-53 0.7034568428116472 1101 54-55 0.7453227364295998 1101 56-57 0.7255210959365073 1101 58-59 0.6718621268034539 1101 60-61 0.7229244502041254 1101 62-63 0.6689120634891594 1101 64-65 0.663436722700574 1101 66-67 0.5434559041235545 1101 68-69 0.6977578782359615 1101 70-71 0.7980595637508543 1101 72-73 0.8368932687235144 1101 74-75 0.8338169340991328 1101 76-77 0.7074879023209668 1101 78-79 0.6480315647058355 1101 80-81 0.6922308208124122 1101 82-83 0.6251204915583557 1101 84-85 0.639652243302681 1101 86-87 0.6499333119302371 1101 88-89 0.6626959709934255 1101 90-91 0.6709772123728257 1101 92-93 0.8289013207422329 1101 94-95 0.7609976912434568 1101 96-97 0.8303633347498369 1101 98-99 0.8567824517696216 1101 100-101 0.5583620419770305 1102 1 0.3620145410951494 1102 2 0.37702929172537836 1102 3 0.4231532394034758 1102 4 0.3298251789588633 1102 5 0.27737215998438103 1102 6 0.3901292356812647 1102 7 0.32143983250578145 1102 8 0.3651342292404678 1102 9 0.534394006619543 1102 10-11 0.5176217181806706 1102 12-13 0.5769016456855312 1102 14-15 0.7125583685881622 1102 16-17 0.647942163462119 1102 18-19 0.5921827772615913 1102 20-21 0.6744635708510671 1102 22-23 0.7732122113781443 1102 24-25 0.7494815887945592 1102 26-27 0.7057377367465207 1102 28-29 0.6700099316624701 1102 30-31 0.7275191363467712 1102 32-33 0.7447142370753141 1102 34-35 0.611285674812585 1102 36-37 0.6581821090590907 1102 38-39 1.0681853434204882 1102 40-41 0.7899590673391543 1102 42-43 0.6175384118003464 1102 44-45 0.7905106529469208 1102 46-47 0.7601056448522243 1102 48-49 0.6844139125383464 1102 50-51 0.6675311905240449 1102 52-53 0.7053078555042873 1102 54-55 0.7694669155899447 1102 56-57 0.7237452938689088 1102 58-59 0.819636966739715 1102 60-61 0.746148926549175 1102 62-63 0.7759810936673546 1102 64-65 0.7414725693863389 1102 66-67 0.7006765397858814 1102 68-69 0.6330813678395657 1102 70-71 0.7003806467713076 1102 72-73 0.5393004322257653 1102 74-75 0.563389955773431 1102 76-77 0.4576371863926738 1102 78-79 0.6053036949420907 1102 80-81 0.5706177497547635 1102 82-83 0.5280692186165652 1102 84-85 0.47705706735452225 1102 86-87 0.4727904875022624 1102 88-89 0.5096655631573412 1102 90-91 0.49679952174212616 1102 92-93 0.39455580454245975 1102 94-95 0.32632851054988876 1102 96-97 0.2811500974916079 1102 98-99 0.36460176871284133 1102 100-101 0.18681684870454518 1103 1 0.23917873520494481 1103 2 0.21321074435142862 1103 3 0.34551157129966015 1103 4 0.15986269923993746 1103 5 0.16319039433752636 1103 6 0.1981050433320135 1103 7 0.2839853764857523 1103 8 0.31912439238184476 1103 9 0.3811981533165394 1103 10-11 0.4041597283971612 1103 12-13 0.3264022899423864 1103 14-15 0.49283730733818487 1103 16-17 0.3910293209939866 1103 18-19 0.44483206371644 1103 20-21 0.5455023605706657 1103 22-23 0.6163515755268847 1103 24-25 0.6365403361703486 1103 26-27 0.5501485720195589 1103 28-29 0.5922789072198 1103 30-31 0.47905543214110935 1103 32-33 0.5834393355431828 1103 34-35 0.5011562152237659 1103 36-37 0.25637276437174705 1103 38-39 0.7308649297264296 1103 40-41 0.6568808204245258 1103 42-43 0.5847407265907734 1103 44-45 0.5085627910807133 1103 46-47 0.45898150614804223 1103 48-49 0.38033499502120804 1103 50-51 0.3405581314211332 1103 52-53 0.45959019745653507 1103 54-55 0.5630851218306248 1103 56-57 0.40748006361646816 1103 58-59 0.43860066461303404 1103 60-61 0.5024533954136885 1103 62-63 0.4104873221090699 1103 64-65 0.4102617683235721 1103 66-67 0.45463757416797534 1103 68-69 0.41423112046400234 1103 70-71 0.3985238329947691 1103 72-73 0.3288868929658051 1103 74-75 0.2278379063705529 1103 76-77 0.3537056794726787 1103 78-79 0.4431899539385036 1103 80-81 0.4143682237029722 1103 82-83 0.34968089738842423 1103 84-85 0.311678653813388 1103 86-87 0.14343803248571518 1103 88-89 0.0859314719863562 1103 90-91 0.18501485128720674 1103 92-93 0.06376739394234221 1103 94-95 0.18204061646537184 1103 96-97 0.3522311497105761 1103 98-99 0.47461302464081356 1103 100-101 0.07530717375054152 1104 1 0.2822190420007118 1104 2 0.3105544840532488 1104 3 0.27741091236930515 1104 4 0.19991996287798486 1104 5 0.24061187525245487 1104 6 0.2475839840773233 1104 7 0.33631891571885575 1104 8 0.44018901169974356 1104 9 0.4141607648817711 1104 10-11 0.3821940804489756 1104 12-13 0.4993032235420074 1104 14-15 0.42291251950946673 1104 16-17 0.47484054104975115 1104 18-19 0.4934761907448362 1104 20-21 0.4981125616524835 1104 22-23 0.4141722312078002 1104 24-25 0.49798448036558796 1104 26-27 0.5452432271578758 1104 28-29 0.5489390239235874 1104 30-31 0.4772176617443904 1104 32-33 0.4958015985604689 1104 34-35 0.5290988320310177 1104 36-37 0.4422273735887998 1104 38-39 0.8778227223174113 1104 40-41 0.6847549998270921 1104 42-43 0.4344107249507303 1104 44-45 0.5044500326011487 1104 46-47 0.4845673625199254 1104 48-49 0.39106863173738304 1104 50-51 0.27700806175752035 1104 52-53 0.3570183151903308 1104 54-55 0.46952797084989584 1104 56-57 0.5431618549751178 1104 58-59 0.3243831578191063 1104 60-61 0.4290798375423108 1104 62-63 0.3939949805075571 1104 64-65 0.3064111871804087 1104 66-67 0.4160217110979616 1104 68-69 0.44582214834323963 1104 70-71 0.4263891990697388 1104 72-73 0.333453269584588 1104 74-75 0.3107265626216531 1104 76-77 0.3341039192595652 1104 78-79 0.2938961697102265 1104 80-81 0.2555544362865625 1104 82-83 0.19385820008101007 1104 84-85 0.2575300289224032 1104 86-87 0.27191366401811123 1104 88-89 0.15609789826631726 1104 90-91 0.24735289681302675 1104 92-93 0.21469202696304635 1104 94-95 0.20209147319745213 1104 96-97 0.03814408016499371 1104 98-99 0.008394509295658992 1104 100-101 -0.0706631429621325 1105 1 0.19097040928866704 1105 2 0.2986244891964276 1105 3 0.1664740575300101 1105 4 0.1805019385819122 1105 5 0.1231996895475902 1105 6 0.2297038418069377 1105 7 0.17213063201837286 1105 8 0.19037336920117554 1105 9 0.27228227422279616 1105 10-11 0.3182437591815841 1105 12-13 0.2786088223598071 1105 14-15 0.29552547017119934 1105 16-17 0.274407355250716 1105 18-19 0.3537255985552221 1105 20-21 0.3860304446923166 1105 22-23 0.3568261823983363 1105 24-25 0.35538920484348324 1105 26-27 0.3053825275410773 1105 28-29 0.3766958748511726 1105 30-31 0.479449510317707 1105 32-33 0.470843432138885 1105 34-35 0.3319503970776978 1105 36-37 0.38487770282449674 1105 38-39 0.8688884463099669 1105 40-41 0.7854982249605627 1105 42-43 0.434663899594149 1105 44-45 0.3498269694415015 1105 46-47 0.3311562054502346 1105 48-49 0.4645794650043058 1105 50-51 0.43074483256745566 1105 52-53 0.28144876279150566 1105 54-55 0.41864783582342113 1105 56-57 0.47353303290726956 1105 58-59 0.4953524653737773 1105 60-61 0.5712614858215161 1105 62-63 0.525446949764401 1105 64-65 0.582285345981397 1105 66-67 0.5226332274655832 1105 68-69 0.3019135519956251 1105 70-71 0.394856098024448 1105 72-73 0.3882121560507912 1105 74-75 0.3749510658705546 1105 76-77 0.3062532597015881 1105 78-79 0.44806033119145994 1105 80-81 0.4777783889983205 1105 82-83 0.44199760187894555 1105 84-85 0.4657084029544336 1105 86-87 0.5255577127841136 1105 88-89 0.4873702185630293 1105 90-91 0.4548217298916164 1105 92-93 0.49891334289167943 1105 94-95 0.4468559374741119 1105 96-97 0.4541951352404361 1105 98-99 0.2627647600073715 1105 100-101 0.0733928669475361 1106 1 0.22085253596341659 1106 2 0.22621760339043107 1106 3 0.29022610244895475 1106 4 0.2601353307688754 1106 5 0.16916939915374485 1106 6 0.23096003798811893 1106 7 0.25674417390330007 1106 8 0.22051334164211767 1106 9 0.17370056243539267 1106 10-11 0.35129319827490235 1106 12-13 0.4015952468962425 1106 14-15 0.45283199882872793 1106 16-17 0.479232169595619 1106 18-19 0.4347992552217832 1106 20-21 0.5072062159262032 1106 22-23 0.4485070051024209 1106 24-25 0.4558255907166995 1106 26-27 0.48174923636305067 1106 28-29 0.544399430024221 1106 30-31 0.4241327210639625 1106 32-33 0.3987304277170196 1106 34-35 0.25920096815352167 1106 36-37 0.14887190982592102 1106 38-39 0.7411414299977039 1106 40-41 0.5261733604524323 1106 42-43 0.44174080356127376 1106 44-45 0.24617815837802226 1106 46-47 0.2799611947453329 1106 48-49 0.2730771824072633 1106 50-51 0.19927870843704198 1106 52-53 0.22853782877967888 1106 54-55 0.2121534394302529 1106 56-57 0.3306231431093707 1106 58-59 0.29561720431689054 1106 60-61 0.42944283370378145 1106 62-63 0.23592198520283603 1106 64-65 0.37992658274018964 1106 66-67 0.2766523932273728 1106 68-69 0.33778475742116143 1106 70-71 0.3438734790676605 1106 72-73 0.25886496720436014 1106 74-75 0.3304630480547601 1106 76-77 0.44215388056525384 1106 78-79 0.5116614223608913 1106 80-81 0.4021826240024211 1106 82-83 0.325461689450961 1106 84-85 0.40136639764054394 1106 86-87 0.39101811899015715 1106 88-89 0.33862330940213425 1106 90-91 0.3784105225414436 1106 92-93 0.30295451904510884 1106 94-95 0.42412503822847114 1106 96-97 0.481201646903898 1106 98-99 0.5079292646507092 1106 100-101 0.21587292280274184 1107 1 0.15218751992903634 1107 2 0.07862000898503396 1107 3 0.11112097852827674 1107 4 0.02638078317172443 1107 5 0.15108974542914666 1107 6 0.17494879702837096 1107 7 0.12489884431312248 1107 8 0.01706493490051031 1107 9 0.19027583913237578 1107 10-11 0.3022701238129386 1107 12-13 0.32146904043711544 1107 14-15 0.3823206188960526 1107 16-17 0.41454063144901454 1107 18-19 0.3618871848460472 1107 20-21 0.32126031115376463 1107 22-23 0.18398642126222597 1107 24-25 0.3187672438697575 1107 26-27 0.3814687601743785 1107 28-29 0.3201732992274202 1107 30-31 0.3993552375212701 1107 32-33 0.4415207849393923 1107 34-35 0.36512266255108017 1107 36-37 0.31100453316911825 1107 38-39 0.7859610788781453 1107 40-41 0.5487619715070622 1107 42-43 0.31526774598344076 1107 44-45 0.30882664452526853 1107 46-47 0.3881609755972235 1107 48-49 0.34260140458603416 1107 50-51 0.38178870164034606 1107 52-53 0.3264210709143427 1107 54-55 0.38606667571774267 1107 56-57 0.3012467056917316 1107 58-59 0.3295439453033069 1107 60-61 0.2685989217548368 1107 62-63 0.3199879534528378 1107 64-65 0.16052961437161883 1107 66-67 0.10203972727016009 1107 68-69 0.19199035465845782 1107 70-71 0.2045815875346264 1107 72-73 0.3579258597796624 1107 74-75 0.40426308434032876 1107 76-77 0.3590510709905068 1107 78-79 0.4689847346876377 1107 80-81 0.35930794393035725 1107 82-83 0.4245382988141735 1107 84-85 0.4537880807559276 1107 86-87 0.5574034051550392 1107 88-89 0.6262224637476415 1107 90-91 0.5716721681921761 1107 92-93 0.5871696728720295 1107 94-95 0.7276426249228614 1107 96-97 0.6348020760700273 1107 98-99 0.8740211229502357 1107 100-101 0.5086744643783412 1108 1 -0.0310001041971546 1108 2 -0.05491828354717754 1108 3 0.007795095944977959 1108 4 -0.05985440115169638 1108 5 0.01743079143307824 1108 6 -0.004565581902028271 1108 7 0.07889949021431875 1108 8 0.08879658583923344 1108 9 0.1709010918348639 1108 10-11 0.26028699098869623 1108 12-13 0.16169266465485777 1108 14-15 0.18341184862581628 1108 16-17 0.20274587799764276 1108 18-19 0.148700183734789 1108 20-21 0.1513478638239505 1108 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-1.2136041795099644 2316 72-73 -1.1195140662332719 2316 74-75 -1.020903581841626 2316 76-77 -0.9349821010431434 2316 78-79 -0.9259202200945786 2316 80-81 -0.7959885112297336 2316 82-83 -0.877096693730202 2316 84-85 -0.6986012720524677 2316 86-87 -0.7356501901853711 2316 88-89 -0.9422597210646515 2316 90-91 -0.7792862402548479 2316 92-93 -0.9924121306625509 2316 94-95 -0.9911094913824208 2316 96-97 -0.9594864132650365 2316 98-99 -1.206003053305846 2316 100-101 -0.4469289303519055 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11831.0 3 2875.0 4 2980.0 5 3300.0 6 3800.0 7 4341.0 8 5488.0 9 6480.0 10 7640.0 11 8996.0 12 10787.0 13 13488.0 14 13605.0 15 13463.0 16 15853.0 17 18508.0 18 17236.0 19 14489.0 20 15368.0 21 16729.0 22 18304.0 23 20463.0 24 23421.0 25 26866.0 26 30943.0 27 36472.0 28 43072.0 29 50909.0 30 61779.0 31 75358.0 32 94294.0 33 122324.0 34 166775.0 35 240238.0 36 379676.0 37 596188.0 38 533966.0 39 68881.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.20116884630209 18.62319944784561 14.541487769242492 23.63414393660981 2 14.951944909627207 19.888747702538346 40.63276713945789 24.526540248376556 3 19.825543582588168 23.51705598707263 29.196702547367398 27.4606978829718 4 13.026618857083497 16.15565099959522 37.09198568578214 33.72574445753914 5 14.347914563340538 36.89183272941452 33.97102479422932 14.789227913015626 6 32.55919989549538 35.28970504288447 17.53197322248209 14.619121839138053 7 29.236278430110584 31.93250118393542 20.546962055719955 18.284258330234042 8 25.888495330642623 35.0984384878485 19.786898591567446 19.226167589941426 9 25.964813545875497 17.817519047813395 20.630351771459175 35.587315634851926 10-11 25.258716351660937 26.182900200458192 27.212270904925546 21.346112542955325 12-13 26.179378365507567 23.398027793998768 27.52775579020762 22.894838050286044 14-15 22.841363134623265 25.151760801171736 25.979426578200464 26.027449486004535 16-17 22.717576944495743 28.31617570216013 26.48409572769973 22.482151625644395 18-19 22.5806203325106 27.507849517662557 27.932236301224577 21.979293848602268 20-21 23.356949233870825 26.436505634513953 27.71126294040342 22.495282191211803 22-23 22.91748731956976 26.343387913018194 27.763893325388217 22.975231442023826 24-25 23.14615225360174 26.47322090576759 27.736992279649066 22.643634560981603 26-27 22.849019724406936 26.43932877282374 28.034510370730608 22.677141132038713 28-29 22.453892249235306 27.209065432960656 27.850651765553124 22.486390552250914 30-31 22.76422968007647 26.51034618084605 27.68614254324284 23.039281595834638 32-33 22.651015483827532 26.628204788462657 27.734021089633448 22.986758638076363 34-35 23.319027309998837 26.560376608485885 27.464963815794196 22.655632265721078 36-37 23.09584780610943 26.309122872993544 27.783060741390457 22.811968579506566 38-39 22.814032460904997 26.74964565172704 27.80876772975637 22.6275541576116 40-41 23.022249125146345 26.616823255328015 27.651101865026067 22.70982575449957 42-43 22.70615557042261 26.65953479051999 27.258310736490106 23.375998902567297 44-45 22.985175609919892 26.522009799370927 27.282199940277955 23.210614650431225 46-47 23.234497262680662 26.865180378642155 27.053204983630675 22.84711737504651 48-49 23.16441060825758 26.715929159993436 27.149241792687345 22.97041843906164 50-51 22.73323057976394 26.93779458372683 27.47763691212774 22.85133792438149 52-53 23.198287021273234 27.038589073313418 26.848154341926247 22.9149695634871 54-55 22.729226638856808 26.612246393049833 27.19487442469791 23.463652543395447 56-57 23.06702622290764 26.81052506124231 27.071935658961483 23.050513056888576 58-59 23.44563514355093 26.49343400533751 27.031922740757626 23.02900811035394 60-61 23.0100808422737 26.59083835402153 27.667754669307293 22.731326134397477 62-63 22.81369900160458 26.72625252876257 27.54554915339768 22.914499316235172 64-65 22.65791294538035 26.93042522735366 27.226464018222412 23.185197809043576 66-67 22.695799317821972 26.76562773884133 26.966398278175763 23.572174665160933 68-69 22.633074211827026 26.693532583695696 27.337244074928318 23.336149129548957 70-71 22.98849645719475 26.55939198561433 27.134111132244676 23.31800042494625 72-73 22.91929656690152 26.34822851356916 27.458160902571883 23.274314016957433 74-75 22.598498578374738 27.136743809136725 27.004863393405405 23.25989421908313 76-77 22.82121958590123 26.57669765647122 27.02131758830766 23.580765169319896 78-79 22.80509149652432 26.628112459028223 27.201344777273505 23.365451267173945 80-81 22.985913530794765 26.79910193013927 27.047227300864172 23.16775723820179 82-83 22.984604738608418 26.602364582011013 27.201185899985298 23.211844779395275 84-85 22.834798218903117 26.729919701970257 27.10529382370775 23.329988255418872 86-87 22.8274627092931 26.72945512016538 27.185294767746583 23.25778740279494 88-89 22.753773063646694 26.669459566667143 26.904336111927652 23.672431257758515 90-91 22.866889600631623 26.500920370403964 27.09565440410125 23.536535624863166 92-93 23.119748891539928 26.533981939186376 27.154119685309773 23.192149483963924 94-95 22.8645355127658 26.39276841662105 27.20545763340187 23.53723843721128 96-97 22.676773817770624 26.285232228775175 27.541633537225696 23.496360416228505 98-99 22.76928807484644 26.507946229041124 26.897404753279773 23.82536094283266 100-101 22.846927647963092 25.824496180378297 27.490017520179087 23.83855865147952 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2824.0 1 1573.5 2 311.0 3 289.0 4 270.0 5 256.0 6 302.5 7 453.0 8 689.0 9 883.5 10 1004.0 11 1114.5 12 1251.0 13 1479.5 14 1688.0 15 1832.5 16 1986.5 17 2081.5 18 2131.0 19 2276.5 20 2491.5 21 2752.0 22 3286.5 23 4024.5 24 4882.0 25 6357.5 26 8601.5 27 11850.0 28 15551.0 29 20160.0 30 25485.5 31 30619.5 32 36714.0 33 42713.5 34 48777.0 35 54490.0 36 60462.5 37 67166.0 38 73280.0 39 77572.5 40 81139.5 41 87464.5 42 93464.5 43 97918.0 44 102764.5 45 107725.5 46 110362.0 47 118837.5 48 138174.5 49 137228.5 50 119313.5 51 109911.0 52 103615.0 53 99885.5 54 97432.5 55 90615.5 56 80129.0 57 71612.5 58 64223.5 59 55027.5 60 45675.0 61 37371.5 62 30185.0 63 23522.0 64 17670.0 65 13301.0 66 10005.5 67 8082.5 68 6359.0 69 4426.5 70 3260.5 71 2536.5 72 1901.5 73 1515.0 74 1187.5 75 894.5 76 621.0 77 389.5 78 247.0 79 174.5 80 126.0 81 92.5 82 77.0 83 51.0 84 38.0 85 26.5 86 13.5 87 13.5 88 11.0 89 10.0 90 9.0 91 6.5 92 4.0 93 2.5 94 3.5 95 5.5 96 6.5 97 4.5 98 3.0 99 2.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2903977480321379 2 0.17824980569841678 3 0.17810680545317137 4 0.19905634138162545 5 0.21392836688714922 6 0.10950243779668083 7 0.1268769675939994 8 0.13871023788805797 9 0.1417132430382118 10-11 0.1286287205982558 12-13 0.11874382864566613 14-15 0.1336873542738126 16-17 0.11103969043306909 18-19 0.13847786248953417 20-21 0.16402128129649743 22-23 0.16823978853123733 24-25 0.1914773283836182 26-27 0.17231529552073183 28-29 0.1696161658917245 30-31 0.1591771479888088 32-33 0.169312290370578 34-35 0.3501361004834123 36-37 0.4680219276576059 38-39 0.4345598702701775 40-41 0.42453197807234244 42-43 0.4474835174342324 44-45 0.45157689945438256 46-47 0.45481228000306023 48-49 0.467074551032855 50-51 0.45997816386255097 52-53 0.4662522996226938 54-55 0.44952127092897964 56-57 0.41262720765566113 58-59 0.40229543993667954 60-61 0.35680348691798003 62-63 0.46405367085204546 64-65 0.46240916803172316 66-67 0.47097130771579276 68-69 0.4825364525500161 70-71 0.4760656914526608 72-73 0.46866542876121037 74-75 0.4732056865477524 76-77 0.4494676458370126 78-79 0.4362043730904998 80-81 0.4330762427257563 82-83 0.44607139001243384 84-85 0.44721539197439725 86-87 0.44889564485603095 88-89 0.4560456571183019 90-91 0.4742066882644703 92-93 0.4783715704072432 94-95 0.47740631875183664 96-97 0.47527919010381103 98-99 0.4720616845857891 100-101 0.4538291533169979 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2797198.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.28618617356011 #Duplication Level Percentage of deduplicated Percentage of total 1 74.30618316905033 40.33799293361726 2 13.991712917425577 15.191134646447418 3 5.091395408729792 8.29177317124544 4 2.2589918495865504 4.905282084448415 5 1.2245338179447152 3.3237635408383595 6 0.7348593580088015 2.39356271521492 7 0.4886317254851902 1.8568166973997868 8 0.33715726617479863 1.4642385697066946 9 0.2391836863488936 1.1685933114133003 >10 1.2124150511731795 11.679644315330112 >50 0.07369048884478133 2.757162524713951 >100 0.037544441044269246 3.6878767508356267 >500 0.002445184763884771 0.9565872529291919 >1k 0.0011234632698447035 1.4113823550970288 >5k 6.608607469674727E-5 0.20954943535044213 >10k+ 6.608607469674727E-5 0.36463969541206886 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10164 0.3633636231686137 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5841 0.20881610811962545 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4463 0.15955252363257802 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4136 0.1478622535837649 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3565 0.12744896857498111 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3138 0.11218369239503247 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2851 0.10192342479867353 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.575006131135515E-5 2 0.0 0.0 0.0 0.0 3.575006131135515E-5 3 0.0 0.0 0.0 0.0 3.575006131135515E-5 4 0.0 3.575006131135515E-5 0.0 0.0 3.575006131135515E-5 5 0.0 7.15001226227103E-5 0.0 0.0 3.575006131135515E-5 6 0.0 7.15001226227103E-5 0.0 3.575006131135515E-5 3.575006131135515E-5 7 0.0 7.15001226227103E-5 0.0 3.575006131135515E-5 3.575006131135515E-5 8 0.0 7.15001226227103E-5 0.0 3.575006131135515E-5 3.575006131135515E-5 9 0.0 7.15001226227103E-5 0.0 3.575006131135515E-5 3.575006131135515E-5 10-11 0.0 7.15001226227103E-5 0.0 5.3625091967032726E-5 3.575006131135515E-5 12-13 0.0 7.15001226227103E-5 0.0 1.0725018393406545E-4 1.6087527590109819E-4 14-15 0.0 7.15001226227103E-5 0.0 1.6087527590109819E-4 2.681254598351636E-4 16-17 0.0 7.15001226227103E-5 0.0 2.145003678681309E-4 3.9325067442490665E-4 18-19 0.0 7.15001226227103E-5 0.0 2.5025042917948604E-4 4.468757663919394E-4 20-21 0.0 1.0725018393406545E-4 0.0 3.0387552114651873E-4 4.647507970476169E-4 22-23 0.0 1.0725018393406545E-4 0.0 3.7537564376922906E-4 4.647507970476169E-4 24-25 0.0 1.0725018393406545E-4 0.0 5.541259503260049E-4 4.647507970476169E-4 26-27 0.0 1.0725018393406545E-4 0.0 7.328762568827806E-4 5.362509196703272E-4 28-29 0.0 1.0725018393406545E-4 0.0 0.0019483783414688555 5.720009809816824E-4 30-31 0.0 1.0725018393406545E-4 0.0 0.005201633920802175 5.720009809816824E-4 32-33 0.0 1.0725018393406545E-4 0.0 0.014174899309952317 5.720009809816824E-4 34-35 0.0 1.0725018393406545E-4 0.0 0.027670547454988884 5.720009809816824E-4 36-37 0.0 1.0725018393406545E-4 0.0 0.04971046025343934 5.720009809816824E-4 38-39 0.0 1.0725018393406545E-4 0.0 0.08546052156479447 5.720009809816824E-4 40-41 0.0 1.0725018393406545E-4 0.0 0.12510733955908734 5.720009809816824E-4 42-43 0.0 1.2512521458974302E-4 0.0 0.1657909093314095 5.898760116373599E-4 44-45 0.0 1.430002452454206E-4 0.0 0.21702074719058145 6.256260729487151E-4 46-47 0.0 1.430002452454206E-4 0.0 0.28380186172019284 6.435011036043926E-4 48-49 0.0 1.430002452454206E-4 0.0 0.35204872876356985 6.435011036043926E-4 50-51 0.0 1.430002452454206E-4 0.0 0.4274992331611849 6.435011036043926E-4 52-53 0.0 2.3237539852380846E-4 0.0 0.5049696160228915 7.507512875384581E-4 54-55 0.0 2.5025042917948604E-4 0.0 0.595310020956686 7.507512875384581E-4 56-57 0.0 2.5025042917948604E-4 0.0 0.7079227140874547 7.686263181941357E-4 58-59 0.0 2.860004904908412E-4 0.0 0.8298482981898314 7.865013488498133E-4 60-61 0.0 2.860004904908412E-4 0.0 0.9612833986010286 8.222514101611685E-4 62-63 1.7875030655677575E-5 2.860004904908412E-4 0.0 1.098760259373845 8.222514101611685E-4 64-65 3.575006131135515E-5 2.860004904908412E-4 0.0 1.248052515410064 8.580014714725236E-4 66-67 3.575006131135515E-5 2.860004904908412E-4 0.0 1.4266598217215942 8.580014714725236E-4 68-69 3.575006131135515E-5 2.860004904908412E-4 0.0 1.6301849207671393 8.580014714725236E-4 70-71 3.575006131135515E-5 2.860004904908412E-4 0.0 1.8492791715137793 8.758765021282011E-4 72-73 3.575006131135515E-5 2.860004904908412E-4 0.0 2.0796525666041514 9.295015940952338E-4 74-75 3.575006131135515E-5 2.860004904908412E-4 0.0 2.3270429908787293 9.65251655406589E-4 76-77 3.575006131135515E-5 2.860004904908412E-4 0.0 2.591182318877677 0.0010188767473736217 78-79 3.575006131135515E-5 3.217505518021963E-4 0.0 2.9057649833869466 0.0010367517780292992 80-81 3.575006131135515E-5 3.217505518021963E-4 0.0 3.250896075286769 0.0010367517780292992 82-83 3.575006131135515E-5 3.217505518021963E-4 0.0 3.5982079209265843 0.0010367517780292992 84-85 3.575006131135515E-5 3.217505518021963E-4 0.0 3.967988680100586 0.0010367517780292992 86-87 3.575006131135515E-5 3.217505518021963E-4 0.0 4.354822218520105 0.0010725018393406543 88-89 3.575006131135515E-5 3.217505518021963E-4 0.0 4.783501203704564 0.0011082519006520096 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9130 0.0 23.85699 1 TACATGG 9260 0.0 22.888231 2 ACATGGG 9455 0.0 21.563665 3 CATGGGG 5540 0.0 20.02727 4 GAGTACT 5315 0.0 18.409117 12-13 TACATCG 325 1.0260919E-8 17.507072 5 AGAGTAC 10955 0.0 17.364803 10-11 GTGTAGC 1615 0.0 17.336172 1 AGTACTT 5815 0.0 17.070667 12-13 GTACTTT 5645 0.0 16.958567 14-15 ATGGGGA 3670 0.0 16.278715 5 GTGTAAG 1150 0.0 16.09313 1 GTATAGG 740 0.0 16.03179 1 GTATAGC 985 0.0 15.898328 1 TAAGGTG 940 0.0 15.636858 5 GAGTACA 8310 0.0 15.30409 1 TATACAG 1600 0.0 15.113527 5 CCGTAGA 255 1.04796774E-4 14.875289 2 GGACCGA 255 1.053419E-4 14.866233 6 GTATCAA 19130 0.0 14.75964 1 >>END_MODULE