FastQCFastQC Report
Fri 27 May 2016
SRR939253_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939253_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2797198
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT112490.4021524396914341No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT55180.19726883831605774No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT42720.1527242619221092No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38640.1381382369070763No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC36940.1320607264841459No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT31590.11293444368257091No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30590.1093594375514354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG95300.022.3279081
TACATGG95750.021.7565822
ACATGGG96600.020.4353053
AGTACTT61350.017.8680812-13
CATGGGG55800.017.858714
GAGTACT58100.017.39738512-13
AACCGAT1952.2940073E-417.034467
AGAGTAC117700.016.97407510-11
GTACTTT60100.016.34467914-15
AGGACCG3705.0227754E-815.3902075
TGCACCG5651.8189894E-1215.1178145
GTGTAGG13950.015.0145361
TACTTTT72650.014.69695614-15
GTATAGC10450.014.5769651
ATGGGGA35550.014.5496265
GAGTACA84000.014.3942111
AGTACAT77200.014.3834652
GTACTAG6300.014.356431
GTGTAGC16050.014.23638251
CTTACAC12450.014.102543
Produced by FastQC (version 0.11.5)