##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939253_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2797198 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.333142666339675 33.0 31.0 34.0 31.0 34.0 2 32.54319965908741 34.0 31.0 34.0 31.0 34.0 3 32.641379337465565 34.0 31.0 34.0 31.0 34.0 4 36.14364803635638 37.0 35.0 37.0 35.0 37.0 5 36.08515235603629 37.0 35.0 37.0 35.0 37.0 6 36.1126816907491 37.0 35.0 37.0 35.0 37.0 7 36.06009156305703 37.0 35.0 37.0 35.0 37.0 8 36.033383764753154 37.0 35.0 37.0 35.0 37.0 9 37.74459083697329 39.0 38.0 39.0 35.0 39.0 10-11 37.665393011149014 39.0 37.0 39.0 35.0 39.0 12-13 37.59613334486869 39.0 37.0 39.0 35.0 39.0 14-15 38.909085449081545 40.0 38.0 41.0 35.0 41.0 16-17 38.91379033590043 40.0 38.0 41.0 35.0 41.0 18-19 39.00075897380164 40.0 38.5 41.0 35.0 41.0 20-21 38.9767049025489 40.0 38.0 41.0 35.0 41.0 22-23 38.902103640857746 40.0 38.0 41.0 35.0 41.0 24-25 38.83697829041777 40.0 38.0 41.0 35.0 41.0 26-27 38.68767620311469 40.0 38.0 41.0 34.0 41.0 28-29 38.53907696201699 40.0 38.0 41.0 34.0 41.0 30-31 38.38476646987449 40.0 38.0 41.0 34.0 41.0 32-33 38.3756691517726 40.0 38.0 41.0 34.0 41.0 34-35 38.29668082130761 40.0 38.0 41.0 34.0 41.0 36-37 38.163251761226775 40.0 38.0 41.0 33.0 41.0 38-39 38.04858004331477 40.0 38.0 41.0 33.0 41.0 40-41 37.897358177719276 40.0 37.5 41.0 33.0 41.0 42-43 37.74524416934375 40.0 37.0 41.0 33.0 41.0 44-45 37.54589771621458 40.0 37.0 41.0 32.5 41.0 46-47 37.31300018804532 40.0 37.0 41.0 32.0 41.0 48-49 37.06234667692455 40.0 36.5 41.0 31.0 41.0 50-51 36.83682867641118 39.5 36.0 41.0 31.0 41.0 52-53 36.59794801798085 39.0 36.0 41.0 30.5 41.0 54-55 36.357004044761936 39.0 35.0 40.0 30.0 41.0 56-57 36.03939388631052 38.5 35.0 40.0 29.5 41.0 58-59 35.72440813985996 38.0 35.0 40.0 29.0 41.0 60-61 35.36706071575913 38.0 34.0 40.0 28.0 41.0 62-63 35.020866417035904 37.5 34.0 40.0 28.0 41.0 64-65 34.7053394146571 37.0 34.0 40.0 27.5 41.0 66-67 34.276329383904894 36.5 33.5 39.0 26.5 40.5 68-69 33.80179594008004 36.0 33.0 39.0 26.0 40.0 70-71 33.30183133264073 35.0 32.5 38.5 26.0 40.0 72-73 32.79892664015919 35.0 32.0 37.5 25.0 39.5 74-75 32.26647648825718 35.0 31.5 37.0 23.5 39.0 76-77 30.303081333534486 33.0 29.5 35.0 20.5 37.5 78-79 31.279700972187168 34.0 31.0 36.0 22.0 38.0 80-81 31.27333835502528 34.0 31.0 36.0 22.0 37.0 82-83 30.90603167884433 34.0 31.0 35.0 20.5 37.0 84-85 30.45582257673572 34.0 30.5 35.0 19.0 36.5 86-87 29.853413129853518 34.0 30.0 35.0 14.5 36.0 88-89 29.25883044389421 34.0 29.0 35.0 7.0 35.5 90-91 28.76489097303802 34.0 29.0 35.0 3.5 35.0 92-93 28.398491097162232 33.0 29.0 35.0 2.0 35.0 94-95 27.85922305106753 33.0 27.5 35.0 2.0 35.0 96-97 27.101835479647846 33.0 26.5 34.5 2.0 35.0 98-99 26.066115269637685 32.5 24.5 34.0 2.0 35.0 100-101 21.61727485862638 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21943456124272842 1101 2 0.23971983840374378 1101 3 0.2625156519691956 1101 4 0.14493069052063134 1101 5 0.16303999926067547 1101 6 0.20926348867933342 1101 7 0.20456379743286845 1101 8 0.2179385578966304 1101 9 0.2964994343980649 1101 10-11 0.3314749912239989 1101 12-13 0.345632964321716 1101 14-15 0.3314191699169555 1101 16-17 0.30542792352752457 1101 18-19 0.3926448719290221 1101 20-21 0.4268960204832908 1101 22-23 0.3635185953388387 1101 24-25 0.3704056116999652 1101 26-27 0.3622638910236162 1101 28-29 0.33635620813650746 1101 30-31 0.28294030273898585 1101 32-33 0.45590172616049074 1101 34-35 0.5299044676564009 1101 36-37 0.46295977922241605 1101 38-39 0.4444059294913032 1101 40-41 0.5226390529314529 1101 42-43 0.4116291850436511 1101 44-45 0.5243066169467809 1101 46-47 0.571251817078199 1101 48-49 0.42943309031767285 1101 50-51 0.5205005867052108 1101 52-53 0.46288623064554457 1101 54-55 0.3610249961466181 1101 56-57 0.3139121217219554 1101 58-59 0.41245960998908515 1101 60-61 0.34840886986286534 1101 62-63 0.3445140495806882 1101 64-65 0.3399848315211216 1101 66-67 0.30941109846048676 1101 68-69 0.3957064864685407 1101 70-71 0.3508423870582291 1101 72-73 0.4686163327704378 1101 74-75 0.41590291837362514 1101 76-77 0.3983514095601812 1101 78-79 0.5041992141139211 1101 80-81 0.5268102040683864 1101 82-83 0.6487322948823397 1101 84-85 0.5824501367680845 1101 86-87 0.6606556950516627 1101 88-89 0.5386577641742285 1101 90-91 0.7181269976491755 1101 92-93 0.6652318569627234 1101 94-95 0.5915305842494973 1101 96-97 0.5382676725602416 1101 98-99 0.4314733760363225 1101 100-101 0.2184605745574224 1102 1 0.23439606422551407 1102 2 0.25802872397050436 1102 3 0.2634115916777233 1102 4 0.17901357341369106 1102 5 0.22312756356419072 1102 6 0.18263845685794422 1102 7 0.20972533244486158 1102 8 0.28028385262111044 1102 9 0.370248667586921 1102 10-11 0.2926267819505526 1102 12-13 0.3350090517975488 1102 14-15 0.33654847180392977 1102 16-17 0.3489654642953681 1102 18-19 0.39823194693213537 1102 20-21 0.37616100327895907 1102 22-23 0.42210398621998735 1102 24-25 0.4448286034703983 1102 26-27 0.3540398954305317 1102 28-29 0.4224677466698381 1102 30-31 0.37510139495724815 1102 32-33 0.4367614455613307 1102 34-35 0.5599654251229538 1102 36-37 0.6150403686227506 1102 38-39 0.5798557949042831 1102 40-41 0.5123738613655178 1102 42-43 0.57602769061873 1102 44-45 0.6175188541154526 1102 46-47 0.7092198659734166 1102 48-49 0.7810856614622992 1102 50-51 0.7523651404474023 1102 52-53 0.7034407878369109 1102 54-55 0.5928886087756666 1102 56-57 0.616316399143912 1102 58-59 0.5477749746492506 1102 60-61 0.6004645354502358 1102 62-63 0.6435463970509474 1102 64-65 0.5888066755099715 1102 66-67 0.60357914581855 1102 68-69 0.6855334926198111 1102 70-71 0.6900433255362515 1102 72-73 0.768737861847157 1102 74-75 0.683263279535204 1102 76-77 0.7940004709802047 1102 78-79 0.7918257408935325 1102 80-81 0.8949444111939009 1102 82-83 0.8201644447815859 1102 84-85 0.6948353582174924 1102 86-87 0.5360018874412269 1102 88-89 0.7484077276668799 1102 90-91 0.8659203461438416 1102 92-93 0.7197700692147926 1102 94-95 0.7451255815430144 1102 96-97 0.5485872918360606 1102 98-99 0.1945157532630155 1102 100-101 0.1066207281721816 1103 1 0.11281484322234547 1103 2 0.15340284872008425 1103 3 0.12740248811958566 1103 4 0.09077500965696572 1103 5 0.16060845905386145 1103 6 0.1295718927178413 1103 7 0.1738946132209307 1103 8 0.1360800770148174 1103 9 0.16410554828299695 1103 10-11 0.1555394608723546 1103 12-13 0.1639311422949632 1103 14-15 0.2233857436696951 1103 16-17 0.14326513965745136 1103 18-19 0.16700948185732756 1103 20-21 0.259758697431991 1103 22-23 0.22383712394397293 1103 24-25 0.24898100739044082 1103 26-27 0.2768410219927304 1103 28-29 0.2784973294812758 1103 30-31 0.3005773484081473 1103 32-33 0.29616178713239094 1103 34-35 0.30068366246245404 1103 36-37 0.33477769069379804 1103 38-39 0.38374208423338274 1103 40-41 0.3560124639918598 1103 42-43 0.21607627920290895 1103 44-45 0.33198720109822233 1103 46-47 0.24076929863493035 1103 48-49 0.17106478976033657 1103 50-51 0.1730502494910624 1103 52-53 0.04356087712758239 1103 54-55 0.14562792389176593 1103 56-57 0.30490111512912677 1103 58-59 0.1373862487768278 1103 60-61 0.3196241293112152 1103 62-63 0.289247575381971 1103 64-65 0.37208471944576615 1103 66-67 0.20876375226212218 1103 68-69 0.121433335968689 1103 70-71 0.051118140792219435 1103 72-73 0.16693863326675995 1103 74-75 0.16014133010041576 1103 76-77 0.26913947363838275 1103 78-79 0.2625822177427324 1103 80-81 0.269048365684867 1103 82-83 0.28631707666583495 1103 84-85 0.34217293802255355 1103 86-87 0.1739267189814626 1103 88-89 0.29502992515736537 1103 90-91 0.4060130422051742 1103 92-93 0.4096284452809904 1103 94-95 0.12963841594327974 1103 96-97 0.004064929220064073 1103 98-99 -0.16781607036774204 1103 100-101 -0.31513681329173693 1104 1 0.07110297312619451 1104 2 0.0711789472822133 1104 3 0.11055355161999358 1104 4 0.04304677895930098 1104 5 0.08930066853209695 1104 6 0.06907491300688662 1104 7 0.10561600171087804 1104 8 0.0871509346358934 1104 9 0.1297274396690824 1104 10-11 0.0786590416338484 1104 12-13 0.09076596214288202 1104 14-15 0.19817786409500115 1104 16-17 0.12217231769127324 1104 18-19 0.08846204086447074 1104 20-21 0.17216655983985163 1104 22-23 0.18908556015311007 1104 24-25 0.16366730657455975 1104 26-27 0.2197090473935006 1104 28-29 0.14444140546901707 1104 30-31 0.13312320453479032 1104 32-33 0.14895637835492437 1104 34-35 0.21167717738610037 1104 36-37 0.21402812867786025 1104 38-39 0.09533904386528036 1104 40-41 0.23447023895618457 1104 42-43 0.3111357576685094 1104 44-45 0.3249216713252778 1104 46-47 0.3073834940700877 1104 48-49 0.3019210160314074 1104 50-51 0.34564708496998975 1104 52-53 0.28616496252642776 1104 54-55 0.3349278544580727 1104 56-57 0.2781640848986484 1104 58-59 0.3078566199022248 1104 60-61 0.34943724078370764 1104 62-63 0.47414321791953284 1104 64-65 0.4566551885140484 1104 66-67 0.2898443735174183 1104 68-69 0.3120370259173626 1104 70-71 0.2906626220397541 1104 72-73 0.3877445239416488 1104 74-75 0.23524115061508866 1104 76-77 0.4129843989243547 1104 78-79 0.4984065550954533 1104 80-81 0.6151613816668764 1104 82-83 0.537294513014448 1104 84-85 0.5000223941252848 1104 86-87 0.4849292005079633 1104 88-89 0.6414963938945775 1104 90-91 0.5844885204623118 1104 92-93 0.5729377195072942 1104 94-95 0.40115879925405906 1104 96-97 0.37693175907379484 1104 98-99 0.41273789708579045 1104 100-101 0.33296763153759557 1105 1 0.06556210660971828 1105 2 0.026652083243149605 1105 3 0.08297019232932001 1105 4 0.08318600690341071 1105 5 0.06401290421994332 1105 6 0.07650354238673884 1105 7 0.09783894027528106 1105 8 0.00954113507486909 1105 9 0.12027848387865703 1105 10-11 0.053615397078011995 1105 12-13 0.09896968985880505 1105 14-15 0.1199287895044634 1105 16-17 0.05391947626770133 1105 18-19 0.08758697657477654 1105 20-21 0.1626411808022752 1105 22-23 0.07857138744382297 1105 24-25 0.08880331097885374 1105 26-27 0.189995978656043 1105 28-29 0.10483822684144428 1105 30-31 0.1766489602556689 1105 32-33 0.1288719143680197 1105 34-35 0.2274790419684578 1105 36-37 0.07843046990947045 1105 38-39 0.1380230572810035 1105 40-41 0.2348911101087623 1105 42-43 0.2471800034688485 1105 44-45 0.2101086004146424 1105 46-47 0.10085962900964063 1105 48-49 0.1840868725875069 1105 50-51 0.21838897722407324 1105 52-53 0.27206976306537456 1105 54-55 0.2607846278758217 1105 56-57 0.2112571618941672 1105 58-59 0.22253893824308335 1105 60-61 0.17048442036747247 1105 62-63 0.2657475265970106 1105 64-65 0.31246987481654287 1105 66-67 0.21960235449490995 1105 68-69 0.2900926881193584 1105 70-71 0.17955589751839796 1105 72-73 0.13870905051592786 1105 74-75 0.32342778270415096 1105 76-77 0.29489599425058444 1105 78-79 0.3369611777964181 1105 80-81 0.26792094906059205 1105 82-83 0.36588586233750675 1105 84-85 0.4542713093498101 1105 86-87 0.3490316438786998 1105 88-89 0.6120361376050525 1105 90-91 0.6771739826599656 1105 92-93 0.4790588231893729 1105 94-95 0.6706590419250311 1105 96-97 0.5450625406192096 1105 98-99 0.6199368945286885 1105 100-101 0.4151631881233975 1106 1 0.16010086776250887 1106 2 0.11190361936373705 1106 3 0.14732311752742788 1106 4 0.0449160441606864 1106 5 0.03236694324624523 1106 6 0.12282910371997247 1106 7 0.07902493525076437 1106 8 0.040603155239800515 1106 9 0.09028592987471029 1106 10-11 0.16199422424150356 1106 12-13 0.16564752665703963 1106 14-15 0.1729790202893895 1106 16-17 0.14913670347704766 1106 18-19 0.14531037181247797 1106 20-21 0.25603833630894 1106 22-23 0.2255995750774602 1106 24-25 0.24687334429133045 1106 26-27 0.2110321118704377 1106 28-29 0.28557998727693246 1106 30-31 0.2713814128548364 1106 32-33 0.2957249470587442 1106 34-35 0.44551398150301225 1106 36-37 0.3458725621046881 1106 38-39 0.30679635161214236 1106 40-41 0.34492416323331554 1106 42-43 0.33904509921917025 1106 44-45 0.3961363355555818 1106 46-47 0.3938445588869186 1106 48-49 0.2712628335543883 1106 50-51 0.32114986520176814 1106 52-53 0.33039375193528286 1106 54-55 0.3408229430195888 1106 56-57 0.4375344982886986 1106 58-59 0.3906547872947854 1106 60-61 0.479816416924983 1106 62-63 0.46121707138116363 1106 64-65 0.39198505925679683 1106 66-67 0.411389168325222 1106 68-69 0.42777325011120126 1106 70-71 0.4353149420831315 1106 72-73 0.36542345527645637 1106 74-75 0.424101403062096 1106 76-77 0.5638480334365887 1106 78-79 0.6313952445592257 1106 80-81 0.5163112557523668 1106 82-83 0.7029385309865894 1106 84-85 0.5997079238581087 1106 86-87 0.6650332327218536 1106 88-89 0.7693666900078711 1106 90-91 0.8152903845308188 1106 92-93 0.9357907611890006 1106 94-95 0.909577304544154 1106 96-97 0.9217572554634934 1106 98-99 1.0994795834273319 1106 100-101 0.8866411330284869 1107 1 0.0681317029265287 1107 2 0.06599116461146082 1107 3 0.07735445588952672 1107 4 0.05635734404469872 1107 5 0.05983258445662898 1107 6 0.07899322141928167 1107 7 0.12411033479825306 1107 8 0.07792328768863399 1107 9 0.09055396306283114 1107 10-11 0.0896999344961884 1107 12-13 0.0732004019097019 1107 14-15 0.15439049479670075 1107 16-17 0.22221357187379454 1107 18-19 0.1671876188729513 1107 20-21 0.1888958922558075 1107 22-23 0.22693648027365754 1107 24-25 0.20262752120706295 1107 26-27 0.18060838857251582 1107 28-29 0.19676624477843774 1107 30-31 0.16582420446418666 1107 32-33 0.2050051690397865 1107 34-35 0.35976266491225317 1107 36-37 0.2973713371734519 1107 38-39 0.2954816338250126 1107 40-41 0.3617569735215156 1107 42-43 0.34026937830445547 1107 44-45 0.33083112531647174 1107 46-47 0.2996061116064368 1107 48-49 0.24210473558049728 1107 50-51 0.4157490160256643 1107 52-53 0.5055091594611554 1107 54-55 0.5136024184858101 1107 56-57 0.46122093676152076 1107 58-59 0.5365218394423081 1107 60-61 0.3495546352359966 1107 62-63 0.41595017880143104 1107 64-65 0.48761721390060586 1107 66-67 0.5482724784137858 1107 68-69 0.6515979180598208 1107 70-71 0.5780522013440006 1107 72-73 0.6357702067468196 1107 74-75 0.5034685082293251 1107 76-77 0.45931443792201776 1107 78-79 0.7069585609762576 1107 80-81 0.6754756887004412 1107 82-83 0.7587693223522898 1107 84-85 0.7736119648464488 1107 86-87 0.8156153212442803 1107 88-89 1.0398145245540498 1107 90-91 1.0105353453438433 1107 92-93 1.0090617090913057 1107 94-95 1.0901962616511476 1107 96-97 0.875101416207233 1107 98-99 0.8797697140279688 1107 100-101 0.5232899955960661 1108 1 0.040458650992512446 1108 2 0.050551328782248106 1108 3 0.023545418867179535 1108 4 0.05932734523987193 1108 5 0.10201870571822269 1108 6 0.057506962077233936 1108 7 0.03678881586241545 1108 8 0.04594087959605275 1108 9 0.10879723895164517 1108 10-11 -0.06923931264747551 1108 12-13 0.04131430684015669 1108 14-15 -0.03312365018523167 1108 16-17 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-0.28861121823901925 2316 96-97 0.0064395817449991455 2316 98-99 -0.032826981648266695 2316 100-101 0.6304303524939456 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 26.0 5 50.0 6 103.0 7 261.0 8 656.0 9 1171.0 10 1920.0 11 3030.0 12 4399.0 13 5958.0 14 7735.0 15 9802.0 16 12677.0 17 16381.0 18 16379.0 19 13179.0 20 13404.0 21 14731.0 22 15803.0 23 18039.0 24 21385.0 25 25336.0 26 30434.0 27 36848.0 28 45261.0 29 54854.0 30 67235.0 31 81774.0 32 102706.0 33 132253.0 34 180428.0 35 261050.0 36 417990.0 37 664394.0 38 491241.0 39 28296.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.33300956914982 19.185412182640288 15.049763953322026 23.43181429488786 2 15.384466884360707 20.428121284228002 39.706949597418564 24.480462233992732 3 19.896196121976352 23.6879191247813 29.429021470771822 26.98686328247053 4 13.40995524807325 16.715155666491967 36.75774828953832 33.11714079589647 5 14.361443129874967 37.0171149843522 33.95201197770054 14.669429908072292 6 32.615853436188644 35.6781321879967 17.19667324229461 14.509341133520042 7 29.243407152443268 31.99412411992287 20.37942970072194 18.38303902691193 8 25.94228224101404 35.37890417482066 19.508272206686836 19.17054137747846 9 25.875286626116562 17.75912895690616 20.453754078188243 35.91183033878903 10-11 25.266677582352052 26.390373509490566 27.05986490766832 21.283084000489062 12-13 26.141731833070093 23.468127747839088 27.455528711231743 22.93461170785908 14-15 22.71513624427031 25.262949412860564 25.875051637131683 26.14686270573744 16-17 22.655959667857573 28.482734446039903 26.399005996589057 22.462299889513467 18-19 22.533337408843966 27.590742227348784 27.943113021596094 21.932807342211156 20-21 23.298837497173512 26.46786649852218 27.760374446209106 22.4729215580952 22-23 22.877107734239765 26.375876859628818 27.82384371789198 22.923171688239446 24-25 23.089820599042326 26.545546650612508 27.804163309140073 22.560469441205093 26-27 22.853310448515145 26.526684843199256 28.030565603810814 22.589439104474785 28-29 22.399719290518583 27.312671466231563 27.85382014430155 22.433789098948303 30-31 22.745190008000865 26.560919176976387 27.735505316391617 22.95838549863113 32-33 22.642211956393503 26.711301809882602 27.765588992985123 22.88089724073877 34-35 23.31683562989135 26.635204959893205 27.480170044084044 22.567789366131397 36-37 23.09109770339836 26.383094197474744 27.854144853709208 22.67166324541769 38-39 22.82260568540285 26.831081575075423 27.81211182039465 22.53420091912708 40-41 23.03094286899694 26.640941272704488 27.632322902256313 22.69579295604225 42-43 22.727336102540015 26.770427904279686 27.238308002564 23.263927990616303 44-45 23.03448574194646 26.55624141091881 27.27949388593271 23.12977896120202 46-47 23.222287366964704 26.95074342465117 27.052649265870393 22.77431994251374 48-49 23.169649608195105 26.793045002776005 27.168226033119748 22.869079355909143 50-51 22.724391596301583 26.981492544143297 27.467908052239437 22.82620780731568 52-53 23.193627902237775 27.15885020529495 26.751066693598407 22.896455198868868 54-55 22.784979826240402 26.71148056018916 27.10760911454963 23.395930499020807 56-57 23.067919479402864 26.87837450139382 27.030367272665522 23.0233387465378 58-59 23.444268927457344 26.557974440619297 26.9748476431318 23.02290898879156 60-61 23.041868484834076 26.698914673033563 27.529895782370467 22.729321059761894 62-63 22.84797794906262 26.816056285661173 27.40599393670723 22.929971828568977 64-65 22.631016448075776 27.018766825998817 27.117705173733775 23.23251155219163 66-67 22.720704423741054 26.82592825631529 26.903720890325257 23.549646429618402 68-69 22.61300765834586 26.733870917287256 27.266771523602017 23.38634990076487 70-71 22.942387346194298 26.61624239685571 27.025080098012367 23.41629015893762 72-73 22.853316068437056 26.350833941680207 27.40097411767061 23.394875872212122 74-75 22.530107818648453 27.160850665863006 26.875171823793604 23.433869691694937 76-77 22.73178641493893 26.592577021098755 26.939978641565578 23.735657922396733 78-79 22.696521119565222 26.678799067493735 27.121206867027052 23.50347294591399 80-81 22.821590410823983 26.74947103268256 27.004583698207725 23.42435485828574 82-83 22.791593587583 26.55519916716657 27.221026184059905 23.432181061190523 84-85 22.592179745588265 26.662735351591127 27.11572437846731 23.6293605243533 86-87 22.602190278008663 26.692503412859047 27.09930869585212 23.60599761328017 88-89 22.391962283867155 26.629303638122632 26.8954057685186 24.083328309491616 90-91 22.49941566756575 26.40082050311381 27.076548982831383 24.023214846489058 92-93 22.674512136788312 26.463464509841632 27.150258937694076 23.711764415675972 94-95 22.354388212775785 26.270360553668347 27.25127073592931 24.123980497626555 96-97 22.11132354591988 26.17367451285179 27.615671110875954 24.099330830352375 98-99 22.116491321396733 26.386536085876017 26.943451038727893 24.553521553999357 100-101 22.13078916011753 25.538799845130235 27.755561029304776 24.574849965447456 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 72.0 1 51.5 2 40.0 3 53.5 4 54.0 5 61.0 6 97.0 7 209.5 8 378.5 9 499.5 10 541.5 11 587.0 12 697.0 13 833.5 14 1014.0 15 1177.5 16 1308.0 17 1462.5 18 1632.5 19 1811.5 20 2010.0 21 2376.5 22 2921.0 23 3675.5 24 4712.5 25 6240.0 26 8523.5 27 11889.0 28 15554.0 29 19959.5 30 25500.0 31 31145.5 32 36808.0 33 42405.0 34 48663.0 35 54374.0 36 60836.0 37 68048.5 38 73738.5 39 77912.0 40 81864.5 41 88073.0 42 93921.0 43 98416.5 44 103372.0 45 108516.0 46 111052.5 47 119724.5 48 139749.0 49 139225.0 50 121190.0 51 111042.5 52 104149.0 53 100351.0 54 97723.0 55 90753.5 56 80534.0 57 71859.0 58 64293.0 59 55477.0 60 45944.0 61 37211.0 62 30023.5 63 23324.0 64 17369.0 65 12991.5 66 9749.0 67 7820.0 68 5934.5 69 4045.0 70 2954.5 71 2209.0 72 1697.0 73 1388.5 74 1105.0 75 894.0 76 602.0 77 307.0 78 180.5 79 118.5 80 78.5 81 50.0 82 30.0 83 17.0 84 8.0 85 7.5 86 7.0 87 3.0 88 2.0 89 1.5 90 1.5 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29068374852262874 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0012333771152417527 16-17 8.758765021282011E-4 18-19 8.937515327838788E-5 20-21 1.7875030655677575E-5 22-23 0.0 24-25 0.0 26-27 5.3625091967032726E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0014657525137655612 36-37 0.007775638335219745 38-39 0.0031281303647435754 40-41 0.003271130609988996 42-43 2.5025042917948604E-4 44-45 0.0015015025750769163 46-47 2.860004904908412E-4 48-49 5.362509196703272E-4 50-51 3.575006131135515E-5 52-53 1.0725018393406545E-4 54-55 0.0 56-57 9.116265634395563E-4 58-59 0.002305878954582407 60-61 8.043763795054909E-4 62-63 0.0013585023298314958 64-65 5.3625091967032726E-5 66-67 6.435011036043927E-4 68-69 0.0019126282801575005 70-71 0.0 72-73 0.0 74-75 3.575006131135515E-5 76-77 0.2573825664111014 78-79 1.7875030655677577E-4 80-81 8.937515327838788E-5 82-83 0.0 84-85 0.0 86-87 0.10676755810636217 88-89 0.3748751429108701 90-91 0.2749179714843211 92-93 0.0 94-95 0.0 96-97 0.0 98-99 8.937515327838788E-5 100-101 3.2175055180219637E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2797198.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.55647388112183 #Duplication Level Percentage of deduplicated Percentage of total 1 72.75186410180835 38.235814454696154 2 14.877363854629303 15.63803569691542 3 5.251735228692535 8.280380561320397 4 2.4095081096890216 5.065410001328892 5 1.326339987486984 3.4853876454923562 6 0.8098748018935891 2.553849832361948 7 0.5106100530479705 1.8785104741517726 8 0.36981592417162706 1.5548976767639242 9 0.2643476755548942 1.2503863535252457 >10 1.3007700407762124 12.144506562428008 >50 0.08332386797053785 3.001959518649537 >100 0.04076611620570508 3.9359848148749315 >500 0.0023171869350693953 0.8838234805822085 >1k 0.0012267460244017043 1.490482783099218 >5k 6.815255691120578E-5 0.19764692870185246 >10k+ 6.815255691120578E-5 0.4029232151082165 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11249 0.4021524396914341 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5518 0.19726883831605774 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4272 0.1527242619221092 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3864 0.1381382369070763 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3694 0.1320607264841459 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3159 0.11293444368257091 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3059 0.1093594375514354 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.575006131135515E-5 2 0.0 0.0 0.0 0.0 3.575006131135515E-5 3 0.0 0.0 0.0 0.0 3.575006131135515E-5 4 0.0 3.575006131135515E-5 0.0 0.0 3.575006131135515E-5 5 0.0 3.575006131135515E-5 0.0 0.0 3.575006131135515E-5 6 0.0 3.575006131135515E-5 0.0 3.575006131135515E-5 3.575006131135515E-5 7 0.0 3.575006131135515E-5 0.0 3.575006131135515E-5 3.575006131135515E-5 8 0.0 3.575006131135515E-5 0.0 3.575006131135515E-5 3.575006131135515E-5 9 0.0 3.575006131135515E-5 0.0 3.575006131135515E-5 3.575006131135515E-5 10-11 0.0 7.15001226227103E-5 0.0 5.3625091967032726E-5 3.575006131135515E-5 12-13 0.0 7.15001226227103E-5 0.0 1.0725018393406545E-4 2.145003678681309E-4 14-15 0.0 7.15001226227103E-5 0.0 1.6087527590109819E-4 2.3237539852380846E-4 16-17 0.0 7.15001226227103E-5 0.0 2.145003678681309E-4 2.5025042917948604E-4 18-19 0.0 7.15001226227103E-5 0.0 2.145003678681309E-4 2.5025042917948604E-4 20-21 0.0 1.0725018393406545E-4 0.0 2.681254598351636E-4 3.575006131135515E-4 22-23 0.0 1.0725018393406545E-4 0.0 3.396255824578739E-4 4.647507970476169E-4 24-25 0.0 1.2512521458974302E-4 0.0 5.183758890146496E-4 4.647507970476169E-4 26-27 0.0 1.430002452454206E-4 0.0 7.328762568827806E-4 4.647507970476169E-4 28-29 0.0 1.430002452454206E-4 0.0 0.0020198784640915656 4.647507970476169E-4 30-31 0.0 1.7875030655677577E-4 0.0 0.0053088841047362395 4.647507970476169E-4 32-33 0.0 2.145003678681309E-4 0.0 0.014246399432575028 4.647507970476169E-4 34-35 0.0 2.145003678681309E-4 0.0 0.02779567266957863 5.362509196703272E-4 36-37 0.0 2.145003678681309E-4 0.0 0.05033608632638805 5.362509196703272E-4 38-39 0.0 2.145003678681309E-4 0.0 0.08639002315888972 5.362509196703272E-4 40-41 0.0 2.145003678681309E-4 0.0 0.12614409133711665 5.362509196703272E-4 42-43 0.0 2.3237539852380846E-4 0.0 0.1673102869371421 5.362509196703272E-4 44-45 0.0 2.5025042917948604E-4 0.0 0.2188797503787719 5.362509196703272E-4 46-47 0.0 2.5025042917948604E-4 0.0 0.2858574902455958 5.541259503260049E-4 48-49 0.0 2.5025042917948604E-4 0.0 0.35433673268749655 5.720009809816824E-4 50-51 0.0 2.5025042917948604E-4 0.0 0.4301089876369138 6.077510422930376E-4 52-53 0.0 2.860004904908412E-4 0.0 0.5083122467555031 6.077510422930376E-4 54-55 0.0 2.860004904908412E-4 0.0 0.5994391530381474 6.435011036043926E-4 56-57 0.0 2.860004904908412E-4 0.0 0.7126953472725206 6.792511649157478E-4 58-59 0.0 2.860004904908412E-4 0.0 0.8354431827850585 6.792511649157478E-4 60-61 0.0 2.860004904908412E-4 0.0 0.9679507850355964 6.792511649157478E-4 62-63 0.0 2.860004904908412E-4 0.0 1.106285647279885 6.792511649157478E-4 64-65 0.0 2.860004904908412E-4 0.0 1.2560069040518405 6.792511649157478E-4 66-67 0.0 3.0387552114651873E-4 0.0 1.4345784603020595 6.792511649157478E-4 68-69 0.0 3.217505518021963E-4 0.0 1.6381393094089156 6.792511649157478E-4 70-71 0.0 3.217505518021963E-4 0.0 1.8578591862285045 6.792511649157478E-4 72-73 0.0 3.217505518021963E-4 0.0 2.0885007067787122 7.328762568827806E-4 74-75 0.0 3.217505518021963E-4 0.0 2.3376786341188573 8.222514101611685E-4 76-77 0.0 3.217505518021963E-4 0.0 2.6021218376389514 8.580014714725236E-4 78-79 0.0 3.217505518021963E-4 0.0 2.916150376197895 8.580014714725236E-4 80-81 0.0 3.217505518021963E-4 0.0 3.26226459478378 8.580014714725236E-4 82-83 0.0 3.217505518021963E-4 0.0 3.6102556915885113 8.937515327838787E-4 84-85 0.0 3.217505518021963E-4 0.0 3.9803939513756266 8.937515327838787E-4 86-87 0.0 3.217505518021963E-4 0.0 4.367888865929405 9.473766247509114E-4 88-89 0.0 3.217505518021963E-4 0.0 4.79629972565403 0.0010188767473736217 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9530 0.0 22.327908 1 TACATGG 9575 0.0 21.756582 2 ACATGGG 9660 0.0 20.435305 3 AGTACTT 6135 0.0 17.86808 12-13 CATGGGG 5580 0.0 17.85871 4 GAGTACT 5810 0.0 17.397385 12-13 AACCGAT 195 2.2940073E-4 17.03446 7 AGAGTAC 11770 0.0 16.974075 10-11 GTACTTT 6010 0.0 16.344679 14-15 AGGACCG 370 5.0227754E-8 15.390207 5 TGCACCG 565 1.8189894E-12 15.117814 5 GTGTAGG 1395 0.0 15.014536 1 TACTTTT 7265 0.0 14.696956 14-15 GTATAGC 1045 0.0 14.576965 1 ATGGGGA 3555 0.0 14.549626 5 GAGTACA 8400 0.0 14.394211 1 AGTACAT 7720 0.0 14.383465 2 GTACTAG 630 0.0 14.35643 1 GTGTAGC 1605 0.0 14.2363825 1 CTTACAC 1245 0.0 14.10254 3 >>END_MODULE