FastQCFastQC Report
Fri 27 May 2016
SRR939252_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939252_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2848097
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT104390.3665254378625447No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT59750.20978920310649532No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT47040.16516291404400904No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC41370.14525488422620436No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37110.130297528490076No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT31540.11074061030926967No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29760.1044908231706996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG95600.023.0048521
TACATGG96650.022.2749462
ACATGGG96600.021.8446753
GAGTACT53500.018.8221912-13
GTACTTT55450.017.94709414-15
CATGGGG54200.017.8481664
AGAGTAC112300.017.82775510-11
AGTACTT57150.017.45423512-13
GTGTAGC16050.016.277361
CATGGGA50100.015.8067074
ATGGGGA35200.015.3571145
GTATAGA10250.015.2927781
GTATAGG6250.015.2000961
TAGGTCG2508.846683E-515.1739055
GGTGCGT4101.1046723E-815.0248177
GTATCAA205450.014.6812791
TAAGGTG10750.014.5563635
GTATAGC10150.014.5074811
ATACCGT2000.00536539914.2157896
TACTTTT70850.013.97921814-15
Produced by FastQC (version 0.11.5)