##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939252_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2848097 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.81061108522638 33.0 31.0 34.0 30.0 34.0 2 31.89850486131617 33.0 31.0 34.0 30.0 34.0 3 31.958758778229814 34.0 31.0 34.0 30.0 34.0 4 35.43832917207525 37.0 35.0 37.0 33.0 37.0 5 35.47787733353183 37.0 35.0 37.0 33.0 37.0 6 35.5675389567139 37.0 35.0 37.0 33.0 37.0 7 35.482357518019924 37.0 35.0 37.0 33.0 37.0 8 35.46151342457789 37.0 35.0 37.0 33.0 37.0 9 37.086865018993386 39.0 37.0 39.0 33.0 39.0 10-11 36.99515360607451 39.0 37.0 39.0 33.0 39.0 12-13 36.892880404003094 39.0 37.0 39.0 33.0 39.0 14-15 38.13558017862454 40.0 38.0 41.0 33.0 41.0 16-17 38.077431175974695 40.0 38.0 41.0 33.0 41.0 18-19 37.99639373237639 40.0 38.0 41.0 33.0 41.0 20-21 37.88365161018041 40.0 38.0 41.0 32.5 41.0 22-23 37.80783291439863 40.0 38.0 41.0 32.0 41.0 24-25 37.83095431791824 40.0 38.0 41.0 32.0 41.0 26-27 37.75517845775617 40.0 38.0 41.0 32.0 41.0 28-29 37.62832305219942 40.0 38.0 41.0 32.0 41.0 30-31 37.489022143557605 40.0 37.0 41.0 32.0 41.0 32-33 37.31700517924776 40.0 37.0 41.0 31.0 41.0 34-35 37.030049187229224 40.0 37.0 41.0 30.5 41.0 36-37 36.79793261957019 40.0 36.0 41.0 30.0 41.0 38-39 36.4920740059064 39.5 35.5 41.0 30.0 41.0 40-41 36.39217888295237 39.0 36.0 41.0 30.0 41.0 42-43 36.2498187386174 39.0 35.5 41.0 29.5 41.0 44-45 36.28145091266204 39.0 36.0 41.0 30.0 41.0 46-47 36.16418857925134 39.0 36.0 41.0 29.0 41.0 48-49 36.01276905245854 39.0 35.5 41.0 28.0 41.0 50-51 34.880922770537666 38.0 34.0 40.0 26.0 40.5 52-53 35.012173391566364 38.5 34.5 39.5 26.5 40.5 54-55 35.52836525581819 39.0 35.0 40.5 27.0 41.0 56-57 35.41892410967745 39.0 35.0 41.0 27.0 41.0 58-59 35.21996476945834 39.0 35.0 40.5 26.0 41.0 60-61 34.9155214868033 38.0 34.0 40.0 26.0 41.0 62-63 34.56818728435162 38.0 34.0 40.0 26.0 41.0 64-65 34.193733043502384 37.0 34.0 40.0 25.0 41.0 66-67 33.81756941564841 37.0 33.5 39.5 23.5 41.0 68-69 33.40647439325276 36.0 33.0 39.0 23.0 41.0 70-71 32.97812223389863 36.0 33.0 39.0 22.0 40.5 72-73 32.53184793214557 35.0 32.0 38.0 21.5 40.0 74-75 32.08104130582631 35.0 32.0 37.0 20.0 39.5 76-77 31.631157400889087 35.0 31.5 37.0 19.0 39.0 78-79 31.194912076379424 35.0 31.0 36.0 18.0 39.0 80-81 30.723173052041417 35.0 31.0 36.0 14.0 37.5 82-83 30.283012657223402 34.0 30.5 35.0 8.5 37.0 84-85 29.863831709383494 34.0 30.0 35.0 7.0 36.5 86-87 29.546393785043136 34.0 30.0 35.0 2.0 36.0 88-89 29.135772236689974 34.0 29.0 35.0 2.0 36.0 90-91 28.731529509001973 34.0 29.0 35.0 2.0 35.5 92-93 28.325177478154714 34.0 29.0 35.0 2.0 35.0 94-95 27.855737532815773 34.0 28.0 35.0 2.0 35.0 96-97 27.286923689747926 34.0 27.0 35.0 2.0 35.0 98-99 26.555164378179533 33.0 25.0 35.0 2.0 35.0 100-101 24.09933316877901 31.0 13.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.210791229601206 1101 2 0.015533136282449789 1101 3 0.3228154094376272 1101 4 0.2716630297667493 1101 5 0.22939842057882487 1101 6 0.2896681194469366 1101 7 0.23989551964203315 1101 8 0.30567558545104845 1101 9 0.4441465579185646 1101 10-11 0.344424893862751 1101 12-13 0.3910359515186457 1101 14-15 0.3195323588566197 1101 16-17 0.46921264121179007 1101 18-19 0.4445957771777955 1101 20-21 0.530173808541818 1101 22-23 0.42912851012918196 1101 24-25 0.5272428827490288 1101 26-27 0.4679645294218844 1101 28-29 0.4376686923035322 1101 30-31 0.5939507485260549 1101 32-33 0.5455808789674919 1101 34-35 0.4613766177881331 1101 36-37 0.5018146300343105 1101 38-39 0.6425756936894018 1101 40-41 0.5921305778738031 1101 42-43 0.5161137209756745 1101 44-45 0.7150267645930128 1101 46-47 0.8006652733419628 1101 48-49 0.6082545896652505 1101 50-51 0.7179436547302274 1101 52-53 0.6758161929453408 1101 54-55 0.6328771594815379 1101 56-57 0.6311636375422722 1101 58-59 0.6234284654564206 1101 60-61 0.6764139795299968 1101 62-63 0.699286958638659 1101 64-65 0.6722284169427226 1101 66-67 0.6512965700959015 1101 68-69 0.605445789737189 1101 70-71 0.461079515220554 1101 72-73 0.507745051175597 1101 74-75 0.5536310859362246 1101 76-77 0.5268271351022165 1101 78-79 0.46136704260253225 1101 80-81 0.4023797234621149 1101 82-83 0.3153777370385171 1101 84-85 0.25181825478224695 1101 86-87 0.2183625353203098 1101 88-89 0.30659006978395453 1101 90-91 0.3940440583977889 1101 92-93 0.4581280951744553 1101 94-95 0.3975056303346278 1101 96-97 0.10981721405388001 1101 98-99 -0.07082107515119063 1101 100-101 -0.2794872242975579 1102 1 0.3899346716874277 1102 2 0.40349788119542396 1102 3 0.4179854528365574 1102 4 0.35327366859809217 1102 5 0.3709432215241648 1102 6 0.47393601624889925 1102 7 0.48344367599673177 1102 8 0.5135180003779354 1102 9 0.4713012076890166 1102 10-11 0.5228233195363785 1102 12-13 0.49945394890122685 1102 14-15 0.6922103166844451 1102 16-17 0.6332499360392703 1102 18-19 0.6341251397316867 1102 20-21 0.5686285418194359 1102 22-23 0.5370975165995517 1102 24-25 0.5737430825189804 1102 26-27 0.6453991898265699 1102 28-29 0.6145760265678106 1102 30-31 0.5959231932777911 1102 32-33 0.5590112874932771 1102 34-35 0.6817604573817775 1102 36-37 0.6104877462006115 1102 38-39 0.6872825006577941 1102 40-41 0.576037300881346 1102 42-43 0.5023289892072924 1102 44-45 0.49384596234953904 1102 46-47 0.4898685163599197 1102 48-49 0.5077607752749813 1102 50-51 0.3939799254531451 1102 52-53 0.5834465583413717 1102 54-55 0.5873641650584602 1102 56-57 0.5614981380402639 1102 58-59 0.44280996194962796 1102 60-61 0.4283514910042925 1102 62-63 0.5154069573199607 1102 64-65 0.5115016928645062 1102 66-67 0.6412072800338606 1102 68-69 0.5965926991402313 1102 70-71 0.5924354435792054 1102 72-73 0.4695692548254087 1102 74-75 0.40937275571058507 1102 76-77 0.43381425939178797 1102 78-79 0.41563635790727105 1102 80-81 0.407589847882047 1102 82-83 0.0959293833939796 1102 84-85 0.27510725763528043 1102 86-87 0.30832449585447463 1102 88-89 0.27020365873814356 1102 90-91 0.20036035790209894 1102 92-93 0.3030893706534421 1102 94-95 0.40063956875467 1102 96-97 0.20857457234802368 1102 98-99 0.036100537901216256 1102 100-101 0.18974366591425706 1103 1 0.35931726993559465 1103 2 0.3756150516193877 1103 3 0.4046634943769085 1103 4 0.34343982758682046 1103 5 0.38196408435005225 1103 6 0.330133407207434 1103 7 0.3259501570877106 1103 8 0.4119151298667916 1103 9 0.39556509473123214 1103 10-11 0.4437079094904277 1103 12-13 0.44154895817539597 1103 14-15 0.5870180287766047 1103 16-17 0.5130097551402812 1103 18-19 0.5834201161567023 1103 20-21 0.6111581256157734 1103 22-23 0.6011367569055963 1103 24-25 0.6391110291998601 1103 26-27 0.6048567244670906 1103 28-29 0.6191486626165812 1103 30-31 0.4658254820700307 1103 32-33 0.5602224498300856 1103 34-35 0.6654086753750548 1103 36-37 0.6416108204487401 1103 38-39 0.7852012717863843 1103 40-41 0.7565672703244104 1103 42-43 0.6880829375739452 1103 44-45 0.7413782895921415 1103 46-47 0.6922121173617555 1103 48-49 0.6129578762464334 1103 50-51 0.6852681900609454 1103 52-53 0.6864936487541726 1103 54-55 0.7538138177056766 1103 56-57 0.7714332940406763 1103 58-59 0.7126576074289872 1103 60-61 0.8178216659531401 1103 62-63 0.70662298085103 1103 64-65 0.7178773627075898 1103 66-67 0.6369125984089976 1103 68-69 0.5935764123345777 1103 70-71 0.618780649737765 1103 72-73 0.6639684431022275 1103 74-75 0.504357137319154 1103 76-77 0.5282027987653635 1103 78-79 0.4225919116712973 1103 80-81 0.34037142864856307 1103 82-83 0.34877646093183046 1103 84-85 0.3548621283052569 1103 86-87 0.35610814022201964 1103 88-89 0.4050965349828495 1103 90-91 0.3267741953396701 1103 92-93 0.30809894263370907 1103 94-95 0.199150512157253 1103 96-97 -0.030322441928124277 1103 98-99 -0.20702994184691548 1103 100-101 -0.20818569395429876 1104 1 0.3730644661884668 1104 2 0.38526854145992573 1104 3 0.4771910135083566 1104 4 0.30307411529368977 1104 5 0.2929327436405842 1104 6 0.30611542552838955 1104 7 0.34936497972125125 1104 8 0.33750803992069933 1104 9 0.4195265302497617 1104 10-11 0.43491439250773567 1104 12-13 0.4677286524491464 1104 14-15 0.48565402936091573 1104 16-17 0.47888561982531996 1104 18-19 0.42401295552790685 1104 20-21 0.5271623383047341 1104 22-23 0.5317490222390333 1104 24-25 0.4841445092390657 1104 26-27 0.3573332695909244 1104 28-29 0.3365827863395765 1104 30-31 0.38008942776686894 1104 32-33 0.49793331988701084 1104 34-35 0.5150500784737844 1104 36-37 0.42280839744576326 1104 38-39 0.5831176635758837 1104 40-41 0.5023578209897721 1104 42-43 0.5420778060098783 1104 44-45 0.6428601524111528 1104 46-47 0.5784353521791203 1104 48-49 0.49513953314318115 1104 50-51 0.4630382450992059 1104 52-53 0.5671942085611619 1104 54-55 0.634107657472704 1104 56-57 0.6683994158222575 1104 58-59 0.7008253233410784 1104 60-61 0.6589693645244026 1104 62-63 0.6664657825247957 1104 64-65 0.6890232117481929 1104 66-67 0.7310198405585098 1104 68-69 0.7247048969917245 1104 70-71 0.7476520001543179 1104 72-73 0.6686582402900072 1104 74-75 0.7672349363540931 1104 76-77 0.7274752970689775 1104 78-79 0.7606510707356442 1104 80-81 0.7480931013782346 1104 82-83 0.712911351797743 1104 84-85 0.6507151742584334 1104 86-87 0.5864559933527893 1104 88-89 0.5944204431707334 1104 90-91 0.7498879096684661 1104 92-93 0.6972457906564777 1104 94-95 0.6072956920682877 1104 96-97 0.7515563395963589 1104 98-99 0.5313831019386619 1104 100-101 0.1886752873100761 1105 1 0.22512136879137046 1105 2 0.29499934631976643 1105 3 0.2760335239099412 1105 4 0.3247379255068168 1105 5 0.31405670120575024 1105 6 0.29598810281059684 1105 7 0.3827923142745533 1105 8 0.3576015119570428 1105 9 0.4508780993645516 1105 10-11 0.4151913490076993 1105 12-13 0.41416303595592296 1105 14-15 0.39433521557437246 1105 16-17 0.4077538809848349 1105 18-19 0.38490201815593394 1105 20-21 0.4489515593464404 1105 22-23 0.5161684765084615 1105 24-25 0.3646302372136532 1105 26-27 0.4407444868042987 1105 28-29 0.4020308512345636 1105 30-31 0.5167161752108527 1105 32-33 0.3809458996628692 1105 34-35 0.49908182038694804 1105 36-37 0.4869561216147744 1105 38-39 0.6166751602300806 1105 40-41 0.6399903018253568 1105 42-43 0.524950038322622 1105 44-45 0.6245016060387343 1105 46-47 0.5874044455794589 1105 48-49 0.5756083756080628 1105 50-51 0.5229153720759641 1105 52-53 0.4831134744867427 1105 54-55 0.5383943435541454 1105 56-57 0.6188023208696478 1105 58-59 0.6779964556347053 1105 60-61 0.6809765750780343 1105 62-63 0.7338678437234663 1105 64-65 0.642570349490704 1105 66-67 0.6836993732153971 1105 68-69 0.6331111068761928 1105 70-71 0.5540168130850631 1105 72-73 0.5811104999609782 1105 74-75 0.5573984085725243 1105 76-77 0.5490188602614694 1105 78-79 0.451335205583014 1105 80-81 0.5322487035550552 1105 82-83 0.5227384054854127 1105 84-85 0.4653612130060587 1105 86-87 0.41270452529499835 1105 88-89 0.39916584041123926 1105 90-91 0.3602784585627603 1105 92-93 0.49757719901325714 1105 94-95 0.39430561397671937 1105 96-97 0.4721312407016143 1105 98-99 0.5119284199723246 1105 100-101 0.26547832402745186 1106 1 0.06170550051547963 1106 2 0.16146942221873317 1106 3 0.13185451847673946 1106 4 0.26567235377607545 1106 5 0.15516117634157922 1106 6 0.1931931129719331 1106 7 0.21818611793263187 1106 8 0.25365020342567135 1106 9 0.3057912095632176 1106 10-11 0.27275822719607845 1106 12-13 0.2316096370923333 1106 14-15 0.38278281210708087 1106 16-17 0.3057394477385955 1106 18-19 0.4123722599542816 1106 20-21 0.39787259124059915 1106 22-23 0.44298631898699625 1106 24-25 0.4056254261315715 1106 26-27 0.3499989764563267 1106 28-29 0.33693960657444677 1106 30-31 0.36754567212097555 1106 32-33 0.31281475620136945 1106 34-35 0.35741909383060744 1106 36-37 0.2581601363798214 1106 38-39 0.31610716222086666 1106 40-41 0.5321525928958195 1106 42-43 0.2853755702375196 1106 44-45 0.3825377721040226 1106 46-47 0.2593353070119946 1106 48-49 0.43827271968337556 1106 50-51 0.40939664618321814 1106 52-53 0.2338529709821202 1106 54-55 0.2607870273914017 1106 56-57 0.36418617056480684 1106 58-59 0.40114149316944037 1106 60-61 0.40740206551807745 1106 62-63 0.4144242287759283 1106 64-65 0.2784340631483744 1106 66-67 0.2879515817509173 1106 68-69 0.2663859606773613 1106 70-71 0.3489220430630837 1106 72-73 0.20858032701087126 1106 74-75 0.15448708179221882 1106 76-77 0.17332415659923583 1106 78-79 0.3331632093987018 1106 80-81 0.41412150020388694 1106 82-83 0.31979369145447123 1106 84-85 0.3253730333370264 1106 86-87 0.20146276842054434 1106 88-89 0.2643354725293605 1106 90-91 0.370396039749771 1106 92-93 0.28887660592296527 1106 94-95 0.26376955159854987 1106 96-97 0.14791615215281695 1106 98-99 0.1524837681536546 1106 100-101 0.3515332367229007 1107 1 0.13380906038602802 1107 2 0.13282504969086872 1107 3 0.040268757958937584 1107 4 0.04389241850098813 1107 5 0.1271964154641978 1107 6 0.058993184888507244 1107 7 0.10823286370753493 1107 8 0.027569656859682823 1107 9 0.1362442839968736 1107 10-11 0.15555398411805044 1107 12-13 0.22304976654216802 1107 14-15 0.263343761405892 1107 16-17 0.19015872138117373 1107 18-19 0.21863439587661304 1107 20-21 0.23304519102484278 1107 22-23 0.1886335681779343 1107 24-25 0.12591129452423644 1107 26-27 0.11016258666847989 1107 28-29 0.17924992660321237 1107 30-31 0.17054818920116332 1107 32-33 0.11555118194750946 1107 34-35 0.2379404890121961 1107 36-37 0.2718242859662965 1107 38-39 0.46502661565488523 1107 40-41 0.416483768731851 1107 42-43 0.22567761985995816 1107 44-45 0.42363449989977653 1107 46-47 0.40148741057186754 1107 48-49 0.2925697351454417 1107 50-51 0.08021649515840323 1107 52-53 0.18668288827805668 1107 54-55 0.19091647722959237 1107 56-57 0.17254647261442813 1107 58-59 0.22054098975340963 1107 60-61 0.17079832585609012 1107 62-63 0.18481437620490482 1107 64-65 0.24606800777260673 1107 66-67 0.04419933435789147 1107 68-69 0.15952152342457282 1107 70-71 -0.01755044884630763 1107 72-73 0.0743929843283837 1107 74-75 0.13533749531253392 1107 76-77 0.02863699370819006 1107 78-79 0.1605256689456276 1107 80-81 0.13678061562999488 1107 82-83 0.14582749224555158 1107 84-85 0.029294284685462912 1107 86-87 0.21683493670533238 1107 88-89 0.14389138766779652 1107 90-91 0.15209506887597968 1107 92-93 0.17671551279099873 1107 94-95 0.04863291010268256 1107 96-97 0.04548896768680066 1107 98-99 0.2673597120587239 1107 100-101 0.16822867002027664 1108 1 0.1609278656375288 1108 2 0.17851061634165788 1108 3 0.12300901917175011 1108 4 0.14832444466036065 1108 5 0.18238891969348714 1108 6 0.11959229714383213 1108 7 0.19198010834535495 1108 8 0.22862441650229925 1108 9 0.28703664651191474 1108 10-11 0.2435733408014471 1108 12-13 0.13778050631301397 1108 14-15 0.12942505589418118 1108 16-17 0.3003896679342404 1108 18-19 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-0.7473104700289142 2316 96-97 -0.6401806307614848 2316 98-99 -0.682474328629258 2316 100-101 -0.33542810418004265 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9664.0 3 2886.0 4 2838.0 5 3121.0 6 3475.0 7 4255.0 8 5157.0 9 6185.0 10 7406.0 11 9063.0 12 11055.0 13 14793.0 14 13568.0 15 13563.0 16 15851.0 17 19314.0 18 17019.0 19 14667.0 20 15484.0 21 16705.0 22 18141.0 23 20634.0 24 23460.0 25 27059.0 26 31367.0 27 36917.0 28 43578.0 29 51725.0 30 62324.0 31 75986.0 32 95487.0 33 122838.0 34 166566.0 35 239109.0 36 376082.0 37 596063.0 38 568998.0 39 85667.0 40 27.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.233806530486916 18.54479551206843 14.579369092371333 23.642028865073318 2 14.935704265280233 19.9302893141097 40.62700248620251 24.50700393440756 3 19.85287862222675 23.546908341799696 29.204103227530155 27.3961098084434 4 13.02281007912725 16.207305806368147 37.08810447553411 33.68177963897049 5 14.382241791683908 36.848746335267116 33.99982632180881 14.769185551240163 6 32.51528138511409 35.25614654390735 17.59241465734179 14.636157413636768 7 29.21032891396729 31.873648410064703 20.519713230165994 18.39630944580201 8 25.80861534526243 35.157647227479046 19.811049606505605 19.22268782075292 9 25.914254403455654 17.813744129282107 20.64728892030791 35.62471254695432 10-11 25.232695426828894 26.170556591123766 27.22182960221357 21.374918379833773 12-13 26.210784468734737 23.37394988985043 27.52890767463187 22.886357966782963 14-15 22.82289034737382 25.148207830179746 25.999870356027294 26.029031466419138 16-17 22.657778197825934 28.349983109262432 26.502893559894662 22.489345133016975 18-19 22.5570304247177 27.510052825094633 27.960952049838188 21.97196470034948 20-21 23.333460999102943 26.41637851096971 27.774736443388605 22.47542404653874 22-23 22.91255196580575 26.354818243329554 27.753328965403217 22.979300825461483 24-25 23.098359206792097 26.451884187696045 27.796541431359557 22.6532151741523 26-27 22.837506134749948 26.458453883110018 28.045924215345053 22.65811576679498 28-29 22.432122904438973 27.211474463902547 27.860392921426257 22.49600971023222 30-31 22.766912130574244 26.527302998698417 27.705965489808023 22.999819380919316 32-33 22.62014858874425 26.623660916325363 27.776674856007215 22.979515638923168 34-35 23.33047707895458 26.557798146408818 27.44793583265271 22.663788941983896 36-37 23.097054032775883 26.308564992490062 27.8262875258936 22.768093448840453 38-39 22.802078654159512 26.74267846919452 27.84632051790197 22.608922358744 40-41 22.99306847773344 26.622804893191503 27.669499100425615 22.71462752864944 42-43 22.745121113984045 26.643332410791658 27.257583089969657 23.353963385254637 44-45 22.997516044926144 26.538711324530546 27.30579851456033 23.157974115982977 46-47 23.215086817993917 26.88372897829612 27.080849576209214 22.820334627500745 48-49 23.178151260800373 26.709569458343886 27.167127572982412 22.94515170787333 50-51 22.679174539593472 26.951568086140025 27.488445453855032 22.880811920411475 52-53 23.168300850536845 27.103434640750912 26.84354055080842 22.88472395790382 54-55 22.71355990643152 26.642403604187077 27.23105625014645 23.412980239234948 56-57 23.024402067096435 26.811008074133074 27.099198857453278 23.065391001317213 58-59 23.446074251132533 26.494538368791403 27.068545928530142 22.990841451545922 60-61 22.98323217667292 26.629378626354928 27.664523120738572 22.722866076233576 62-63 22.824832898987854 26.740759962771897 27.5458537931987 22.888553345041547 64-65 22.629470880627377 26.94751945171307 27.24535054143017 23.177659126229383 66-67 22.675837664180065 26.80181728144424 26.98456755856556 23.537777495810136 68-69 22.60961354007416 26.708997611085238 27.337581467915328 23.34380738092527 70-71 22.97024554934649 26.570055546693855 27.153723250032385 23.305975653927266 72-73 22.904656682677725 26.352341892909465 27.51342424927559 23.22957717513722 74-75 22.64432778033311 27.11328578653992 26.981818374068844 23.260568059058123 76-77 22.831139454041303 26.558252785569188 27.040596707445502 23.570011052944007 78-79 22.82413100795309 26.663834016569243 27.216024625735223 23.296010349742446 80-81 23.003458674007028 26.76829420819195 27.03219557978855 23.196051538012473 82-83 22.93063576278885 26.56400140805318 27.30352883637231 23.201833992785666 84-85 22.87366441918081 26.72759727863157 27.09635598597276 23.302382316214864 86-87 22.83705201646108 26.72929248296404 27.201725742406445 23.231929758168434 88-89 22.7435314176705 26.673112473693866 26.905605578988713 23.677750529646925 90-91 22.873669457122997 26.525268505632376 27.096772715287443 23.504289321957184 92-93 23.10447833796535 26.516867636144582 27.17452664501553 23.20412738087454 94-95 22.84582086471893 26.374937366857758 27.290795611423725 23.488446156999586 96-97 22.645423919047584 26.331707925791736 27.571015081286237 23.45185307387444 98-99 22.751630742139454 26.563001993036266 26.885687412466513 23.799679852357762 100-101 22.814085421269017 25.926034905564965 27.480882002106583 23.778997671059436 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1718.0 1 939.0 2 144.5 3 129.5 4 132.5 5 136.0 6 187.5 7 360.5 8 603.5 9 781.5 10 884.5 11 1006.0 12 1197.5 13 1435.0 14 1656.0 15 1907.0 16 2125.5 17 2226.0 18 2290.5 19 2409.0 20 2610.0 21 2832.5 22 3272.0 23 4049.0 24 5040.0 25 6545.5 26 8947.5 27 12278.0 28 15971.0 29 20619.0 30 26246.5 31 31590.5 32 37232.0 33 43109.5 34 49348.5 35 55329.0 36 61968.5 37 69182.5 38 75164.5 39 79340.0 40 83025.5 41 89348.5 42 95300.0 43 99652.5 44 104728.0 45 109830.5 46 112620.5 47 121253.5 48 140954.5 49 140358.0 50 121742.5 51 111465.5 52 104852.5 53 101013.0 54 98662.5 55 92074.0 56 81856.0 57 72867.5 58 65044.0 59 56009.0 60 46462.0 61 38233.0 62 30927.0 63 24061.0 64 18000.5 65 13319.5 66 10112.0 67 8259.5 68 6365.0 69 4389.0 70 3283.0 71 2546.0 72 1913.0 73 1506.0 74 1162.0 75 911.0 76 636.5 77 374.0 78 238.0 79 169.0 80 127.5 81 87.5 82 65.5 83 48.5 84 31.0 85 19.0 86 20.0 87 17.5 88 17.0 89 13.0 90 6.5 91 4.0 92 3.0 93 3.5 94 4.5 95 7.0 96 5.0 97 3.0 98 2.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28527820506113377 2 0.11221527918466259 3 0.12313485109531032 4 0.12517129858990056 5 0.13187753085656845 6 0.05828453174172087 7 0.0671325449940785 8 0.07229388605795378 9 0.06952010412566707 10-11 0.0643763186436417 12-13 0.06906365899756925 14-15 0.06462209678954053 16-17 0.06539454239093682 18-19 0.07045054996371261 20-21 0.07457611169844285 22-23 0.08270434609495392 24-25 0.09176302632951054 26-27 0.09221947145760837 28-29 0.08563612826388989 30-31 0.0817739002569084 32-33 0.07447077820734337 34-35 0.24449658842377908 36-37 0.3694747756133306 38-39 0.34135073348976525 40-41 0.3249362644600939 42-43 0.3484256329752814 44-45 0.3610832074890708 46-47 0.36845655186603543 48-49 0.37753278768244203 50-51 0.37138833403497146 52-53 0.3720027793997185 54-55 0.357273646227639 56-57 0.3344689454045982 58-59 0.3198627013054682 60-61 0.2761668580810275 62-63 0.3482500771567822 64-65 0.3774625653550423 66-67 0.3824307950185685 68-69 0.39478992464090934 70-71 0.39656303840775087 72-73 0.37876167841193614 74-75 0.3824307950185685 76-77 0.3648576575868027 78-79 0.34287806911070795 80-81 0.3414911781445646 82-83 0.35588675526149566 84-85 0.36282121009221246 86-87 0.3556760882792967 88-89 0.3719501126541687 90-91 0.3962294823526024 92-93 0.39115591919797676 94-95 0.39115591919797676 96-97 0.39570281489710496 98-99 0.3851167990416057 100-101 0.3625578763644637 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2848097.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.098693949726076 #Duplication Level Percentage of deduplicated Percentage of total 1 74.22354825137126 40.15397020713659 2 14.133528229462438 15.292108362310044 3 4.917859908364013 7.981493942106381 4 2.3121682903022864 5.0034113878929904 5 1.235295058565922 3.3413924655483376 6 0.7380031175029075 2.3955002872640114 7 0.5024715295063543 1.902813744525051 8 0.3338448446800573 1.4448456063232205 9 0.2487117978142188 1.2109485088473817 >10 1.238054101752672 11.813599246061253 >50 0.07534594750828287 2.832629308088345 >100 0.037594518783112416 3.7041913341982693 >500 0.0022746209732268275 0.8928096806836735 >1k 0.0011698050718504092 1.4531977053462297 >5k 6.498917065835607E-5 0.21007079789620212 >10k+ 6.498917065835607E-5 0.3670174157721262 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10439 0.3665254378625447 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5975 0.20978920310649532 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4704 0.16516291404400904 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4137 0.14525488422620436 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3711 0.130297528490076 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3154 0.11074061030926967 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2976 0.1044908231706996 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.511116369983185E-5 2 0.0 0.0 0.0 0.0 3.511116369983185E-5 3 0.0 0.0 0.0 0.0 3.511116369983185E-5 4 0.0 0.0 0.0 0.0 3.511116369983185E-5 5 0.0 0.0 0.0 0.0 3.511116369983185E-5 6 0.0 0.0 0.0 0.0 3.511116369983185E-5 7 0.0 0.0 0.0 0.0 3.511116369983185E-5 8 0.0 0.0 0.0 0.0 3.511116369983185E-5 9 0.0 0.0 0.0 0.0 3.511116369983185E-5 10-11 0.0 0.0 0.0 0.0 7.02223273996637E-5 12-13 0.0 0.0 0.0 0.0 2.633337277487389E-4 14-15 0.0 0.0 0.0 3.511116369983185E-5 3.5111163699831855E-4 16-17 0.0 0.0 0.0 5.266674554974778E-5 3.862228006981504E-4 18-19 0.0 3.511116369983185E-5 0.0 1.2288907294941148E-4 3.862228006981504E-4 20-21 0.0 7.02223273996637E-5 0.0 1.5800023664924332E-4 4.2133396439798223E-4 22-23 0.0 7.02223273996637E-5 0.0 2.2822256404890704E-4 4.2133396439798223E-4 24-25 0.0 7.02223273996637E-5 0.0 4.564451280978141E-4 4.2133396439798223E-4 26-27 0.0 7.02223273996637E-5 0.0 7.19778855846553E-4 4.564451280978141E-4 28-29 0.0 7.02223273996637E-5 0.0 0.0017731137668415085 4.564451280978141E-4 30-31 0.0 1.5800023664924332E-4 0.0 0.005354452464224358 4.564451280978141E-4 32-33 0.0 1.7555581849915927E-4 0.0 0.014518466189880471 4.564451280978141E-4 34-35 0.0 1.7555581849915927E-4 0.0 0.027737819322867164 4.564451280978141E-4 36-37 0.0 2.1066698219899112E-4 0.0 0.05001585269041048 4.564451280978141E-4 38-39 0.0 2.1066698219899112E-4 0.0 0.08312568005935192 4.915562917976459E-4 40-41 0.0 2.1066698219899112E-4 0.0 0.11914973401537939 4.915562917976459E-4 42-43 0.0 2.4577814589882296E-4 0.0 0.15998401739828383 4.915562917976459E-4 44-45 0.0 2.808893095986548E-4 0.0 0.2120889843288343 5.442230373473938E-4 46-47 0.0 2.808893095986548E-4 0.0 0.276886636936874 6.144453647470573E-4 48-49 0.0 2.808893095986548E-4 0.0 0.34561673987929487 6.320009465969733E-4 50-51 0.0 2.808893095986548E-4 0.0 0.42179040952608005 6.671121102968052E-4 52-53 0.0 2.808893095986548E-4 0.0 0.4983503019735634 7.373344376964689E-4 54-55 0.0 2.808893095986548E-4 0.0 0.5877257691714854 7.373344376964689E-4 56-57 0.0 2.808893095986548E-4 0.0 0.6966230433865139 7.373344376964689E-4 58-59 0.0 3.1600047329848665E-4 0.0 0.8198456723910738 7.373344376964689E-4 60-61 0.0 3.1600047329848665E-4 0.0 0.951161424628445 7.548900195463848E-4 62-63 0.0 3.1600047329848665E-4 0.0 1.0909214117356254 7.724456013963008E-4 64-65 0.0 3.5111163699831855E-4 0.0 1.2429351949740477 7.724456013963008E-4 66-67 0.0 3.5111163699831855E-4 0.0 1.4214403512239926 7.724456013963008E-4 68-69 0.0 3.5111163699831855E-4 0.0 1.625172878592267 7.724456013963008E-4 70-71 0.0 3.5111163699831855E-4 0.0 1.845074096844314 7.900011832462166E-4 72-73 0.0 3.5111163699831855E-4 0.0 2.0714533247989797 8.251123469460485E-4 74-75 0.0 3.5111163699831855E-4 0.0 2.3106656830859342 8.426679287959645E-4 76-77 0.0 3.5111163699831855E-4 0.0 2.5782303060605027 8.426679287959645E-4 78-79 0.0 3.5111163699831855E-4 0.0 2.8886305487488664 8.953346743457123E-4 80-81 0.0 3.5111163699831855E-4 0.0 3.230051504566031 9.128902561956282E-4 82-83 0.0 3.5111163699831855E-4 0.0 3.574720243025431 9.480014198954601E-4 84-85 0.0 3.5111163699831855E-4 0.0 3.94651235544295 9.480014198954601E-4 86-87 0.0 3.5111163699831855E-4 0.0 4.331190264938308 0.0010006681654452078 88-89 1.7555581849915925E-5 3.5111163699831855E-4 0.0 4.764146024520935 0.0010533349109949556 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9560 0.0 23.004852 1 TACATGG 9665 0.0 22.274946 2 ACATGGG 9660 0.0 21.844675 3 GAGTACT 5350 0.0 18.82219 12-13 GTACTTT 5545 0.0 17.947094 14-15 CATGGGG 5420 0.0 17.848166 4 AGAGTAC 11230 0.0 17.827755 10-11 AGTACTT 5715 0.0 17.454235 12-13 GTGTAGC 1605 0.0 16.27736 1 CATGGGA 5010 0.0 15.806707 4 ATGGGGA 3520 0.0 15.357114 5 GTATAGA 1025 0.0 15.292778 1 GTATAGG 625 0.0 15.200096 1 TAGGTCG 250 8.846683E-5 15.173905 5 GGTGCGT 410 1.1046723E-8 15.024817 7 GTATCAA 20545 0.0 14.681279 1 TAAGGTG 1075 0.0 14.556363 5 GTATAGC 1015 0.0 14.507481 1 ATACCGT 200 0.005365399 14.215789 6 TACTTTT 7085 0.0 13.979218 14-15 >>END_MODULE