FastQCFastQC Report
Fri 27 May 2016
SRR939252_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939252_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2848097
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT113730.3993192647581877No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT55580.19514784784366546No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT41880.1470455535748958No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38140.13391397835115867No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC37680.13229886482096642No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT32440.11390061504225452No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32250.11323350293195773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG94100.022.6502321
GCGATAT1503.059458E-522.1438649
TACATGG95350.022.0440582
ACATGGG95950.021.1644653
GAGTACT59800.019.28198412-13
CATGGGG53500.018.978794
GTACTTT61200.018.80212614-15
CCGTCCG1300.007610837518.2504379
AGTACTT63250.017.51753412-13
CCTATAC6000.017.3972233
AGAGTAC119950.017.36644410-11
GTGTAGC15900.016.7561861
GTATAGG8100.015.8585321
GTATAGC10600.015.7089241
TCGCGCT3150.015.06372160-61
TAGCCCT18900.015.0625314
ACTTTTT76850.014.78666216-17
GTGTAAG13350.014.6112321
CGCGGAA2951.8189894E-1214.55404482-83
ATGGGGA34150.014.4494445
Produced by FastQC (version 0.11.5)