##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939252_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2848097 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.29532210454911 33.0 31.0 34.0 31.0 34.0 2 32.499469996983954 34.0 31.0 34.0 31.0 34.0 3 32.59770506411825 34.0 31.0 34.0 31.0 34.0 4 36.11568601771639 37.0 35.0 37.0 35.0 37.0 5 36.04809316536621 37.0 35.0 37.0 35.0 37.0 6 36.082236665394475 37.0 35.0 37.0 35.0 37.0 7 36.03270956010277 37.0 35.0 37.0 35.0 37.0 8 36.00936625402857 37.0 35.0 37.0 35.0 37.0 9 37.72364389274663 39.0 37.0 39.0 35.0 39.0 10-11 37.63900773042491 39.0 37.0 39.0 35.0 39.0 12-13 37.56304771220924 39.0 37.0 39.0 35.0 39.0 14-15 38.87661445519588 40.0 38.0 41.0 35.0 41.0 16-17 38.82484831099502 40.0 38.0 41.0 35.0 41.0 18-19 38.924972534292195 40.0 38.0 41.0 35.0 41.0 20-21 38.92394044163524 40.0 38.0 41.0 35.0 41.0 22-23 38.87068786631916 40.0 38.0 41.0 35.0 41.0 24-25 38.80549661756604 40.0 38.0 41.0 34.0 41.0 26-27 38.64757854103986 40.0 38.0 41.0 34.0 41.0 28-29 38.511091089945324 40.0 38.0 41.0 34.0 41.0 30-31 38.36283736122752 40.0 38.0 41.0 34.0 41.0 32-33 38.2007472006747 40.0 38.0 41.0 33.5 41.0 34-35 38.176596337835406 40.0 38.0 41.0 33.0 41.0 36-37 38.117637145083194 40.0 38.0 41.0 33.0 41.0 38-39 38.02981920910699 40.0 38.0 41.0 33.0 41.0 40-41 37.88965509250563 40.0 37.5 41.0 33.0 41.0 42-43 37.746354320095136 40.0 37.0 41.0 33.0 41.0 44-45 37.572452939629514 40.0 37.0 41.0 32.5 41.0 46-47 37.36097260732342 40.0 37.0 41.0 32.0 41.0 48-49 37.11638701209966 40.0 36.5 41.0 31.0 41.0 50-51 36.8990603199259 39.5 36.0 41.0 31.0 41.0 52-53 36.6900976336129 39.0 36.0 41.0 31.0 41.0 54-55 36.41618895002523 39.0 35.0 40.5 30.0 41.0 56-57 36.17029353283965 39.0 35.0 40.0 29.5 41.0 58-59 35.87705053584902 38.0 35.0 40.0 29.0 41.0 60-61 35.52842160923592 38.0 34.5 40.0 28.0 41.0 62-63 35.16243144106398 37.5 34.0 40.0 28.0 41.0 64-65 34.74007749736052 37.0 34.0 40.0 27.5 41.0 66-67 34.35329836027354 36.5 34.0 39.0 26.5 40.5 68-69 33.95933583020522 36.0 33.0 39.0 26.0 40.0 70-71 33.53627580099976 35.5 33.0 38.5 26.0 40.0 72-73 33.02786421951219 35.0 32.5 37.5 25.5 40.0 74-75 32.51750853289056 35.0 32.0 37.0 24.5 39.0 76-77 30.502824166452193 33.5 29.5 35.0 21.5 37.5 78-79 31.50252308822347 34.0 31.0 36.0 22.5 38.0 80-81 31.5348128943642 35.0 31.0 36.0 23.0 37.0 82-83 31.22164238085992 34.0 31.0 35.0 22.5 37.0 84-85 30.840409227635156 34.0 31.0 35.0 20.5 36.5 86-87 30.313352389332245 34.0 30.5 35.0 18.5 36.0 88-89 29.818335541240344 34.0 30.0 35.0 14.0 36.0 90-91 29.390343446869963 34.0 29.5 35.0 7.5 35.0 92-93 29.03374604165518 34.0 29.0 35.0 4.0 35.0 94-95 28.62757869552898 34.0 29.0 35.0 2.0 35.0 96-97 28.061719281330657 33.5 29.0 35.0 2.0 35.0 98-99 27.291951257278107 33.0 27.0 35.0 2.0 35.0 100-101 23.831302796217965 30.0 13.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2595255999116688 1101 2 0.21698394068381077 1101 3 0.26957973658528545 1101 4 0.13372953690795697 1101 5 0.2342917782784255 1101 6 0.2282804360669033 1101 7 0.20985217612862073 1101 8 0.23221566021821616 1101 9 0.3207306240435557 1101 10-11 0.23224099025031109 1101 12-13 0.2771503114512299 1101 14-15 0.337113591486947 1101 16-17 0.3129735761224808 1101 18-19 0.3211282001896336 1101 20-21 0.3505867389807378 1101 22-23 0.38163423502084015 1101 24-25 0.3960564524635757 1101 26-27 0.3546636358471673 1101 28-29 0.3871245343342693 1101 30-31 0.30967231905232495 1101 32-33 0.34639530713679534 1101 34-35 0.4316484411299939 1101 36-37 0.42707105602035966 1101 38-39 0.4792227868517642 1101 40-41 0.5167891673555332 1101 42-43 0.5374619359696524 1101 44-45 0.6961783279083491 1101 46-47 0.6156016668553264 1101 48-49 0.5672578376445543 1101 50-51 0.5024240387672876 1101 52-53 0.4807946400687584 1101 54-55 0.561110970482261 1101 56-57 0.3910622143375093 1101 58-59 0.311400555476979 1101 60-61 0.39852501770296556 1101 62-63 0.45988704242333966 1101 64-65 0.5460583699752206 1101 66-67 0.4900781679391599 1101 68-69 0.4649262774930918 1101 70-71 0.370511729841958 1101 72-73 0.27226915646154737 1101 74-75 0.17003857370408326 1101 76-77 0.3420704532646077 1101 78-79 0.661236113047341 1101 80-81 0.6876060817082426 1101 82-83 0.5668510533513818 1101 84-85 0.5688318162757895 1101 86-87 0.6065006156405488 1101 88-89 0.733569370652102 1101 90-91 0.7768600050602998 1101 92-93 0.638817765659077 1101 94-95 0.916278650582143 1101 96-97 0.6162630644558398 1101 98-99 0.5753685275793323 1101 100-101 0.47647934965948835 1102 1 0.18861936415529357 1102 2 0.17441948122561968 1102 3 0.20939983171872711 1102 4 0.15797474371447606 1102 5 0.18113089133007065 1102 6 0.07865047172304429 1102 7 0.16052185159394128 1102 8 0.27124505347104133 1102 9 0.2932113676414616 1102 10-11 0.2450614274576779 1102 12-13 0.3338970010317084 1102 14-15 0.30720299345646396 1102 16-17 0.24359495292914346 1102 18-19 0.2502183350018399 1102 20-21 0.29900140333545266 1102 22-23 0.29905385748133284 1102 24-25 0.22929005204594688 1102 26-27 0.43411506543483824 1102 28-29 0.42179437554362664 1102 30-31 0.36629454938027806 1102 32-33 0.29474630521531964 1102 34-35 0.40739710965794984 1102 36-37 0.4826731892791756 1102 38-39 0.4312021577684675 1102 40-41 0.5275594294881998 1102 42-43 0.479284191824604 1102 44-45 0.4386672195798056 1102 46-47 0.5825175091117671 1102 48-49 0.4573179742986966 1102 50-51 0.48980942056684285 1102 52-53 0.48661624807019876 1102 54-55 0.47994644926324526 1102 56-57 0.4591538260258474 1102 58-59 0.3918226311306654 1102 60-61 0.24446744947646692 1102 62-63 0.24022969401939775 1102 64-65 0.3086215712305176 1102 66-67 0.24203964878347506 1102 68-69 0.35697367907727795 1102 70-71 0.20261060281276855 1102 72-73 0.2655137260003926 1102 74-75 0.3499145560323811 1102 76-77 0.471047289325913 1102 78-79 0.4741875424837865 1102 80-81 0.49911995384880825 1102 82-83 0.5641828163507192 1102 84-85 0.5589968132527758 1102 86-87 0.6136111254758525 1102 88-89 0.8489518688683866 1102 90-91 0.854129087778972 1102 92-93 0.857397172930586 1102 94-95 0.8197166446195929 1102 96-97 0.5948869988018437 1102 98-99 0.8054474102467708 1102 100-101 0.7288210134452946 1103 1 0.20776467836713408 1103 2 0.20182732158592387 1103 3 0.1682219667576561 1103 4 0.12190549455841193 1103 5 0.1175981507656303 1103 6 0.0998099026504633 1103 7 0.12926596687162828 1103 8 0.07860428790514362 1103 9 0.13029457889344087 1103 10-11 0.21314517536625033 1103 12-13 0.1958171246311835 1103 14-15 0.205835737450208 1103 16-17 0.23563752989153386 1103 18-19 0.2281099785006262 1103 20-21 0.23384162165602618 1103 22-23 0.2304430339842014 1103 24-25 0.16401784020857946 1103 26-27 0.25736455419723825 1103 28-29 0.15903832807958906 1103 30-31 0.2108720767049448 1103 32-33 0.2544793877479279 1103 34-35 0.32134885749192676 1103 36-37 0.3899932874897374 1103 38-39 0.3268356110874606 1103 40-41 0.4251845886953163 1103 42-43 0.3257865685755874 1103 44-45 0.37891747181442526 1103 46-47 0.3286932292513782 1103 48-49 0.19922052119147082 1103 50-51 0.3237685023064998 1103 52-53 0.031344875510804115 1103 54-55 0.1602348630760133 1103 56-57 0.30849011959384853 1103 58-59 0.08281010934402389 1103 60-61 0.05048286150779546 1103 62-63 0.11388211141068183 1103 64-65 0.287646616007045 1103 66-67 0.33860624985209853 1103 68-69 0.09240874693846024 1103 70-71 0.15777849450439874 1103 72-73 0.20228969834452926 1103 74-75 0.2783910018014879 1103 76-77 0.44170098759375165 1103 78-79 0.4955783764854296 1103 80-81 0.29924379999201633 1103 82-83 0.4404573757598662 1103 84-85 0.2762294761463977 1103 86-87 0.13592591728377812 1103 88-89 0.4724915552852629 1103 90-91 0.6053716102334192 1103 92-93 0.4217059447991893 1103 94-95 0.48613561398624583 1103 96-97 0.30287910597914447 1103 98-99 0.12471032865992626 1103 100-101 0.3754576897120181 1104 1 0.23421355592584803 1104 2 0.17753308835185777 1104 3 0.14839900336186673 1104 4 0.12082581630606626 1104 5 0.12594695575677406 1104 6 0.06288172569868067 1104 7 0.16994975131765955 1104 8 0.21481279715986545 1104 9 0.19343249026675835 1104 10-11 0.2616399872560251 1104 12-13 0.22024388792895166 1104 14-15 0.27629166443026776 1104 16-17 0.3414937094444497 1104 18-19 0.23640047026373168 1104 20-21 0.2858867666969189 1104 22-23 0.26806331237979464 1104 24-25 0.1975918285035192 1104 26-27 0.23915021126362035 1104 28-29 0.3277997551162031 1104 30-31 0.3513548277698959 1104 32-33 0.47610676268666197 1104 34-35 0.4926304173177627 1104 36-37 0.4295791339857544 1104 38-39 0.45069761960708377 1104 40-41 0.5113821421314384 1104 42-43 0.5952310850116689 1104 44-45 0.5351495066280094 1104 46-47 0.5465970984224029 1104 48-49 0.571013588160973 1104 50-51 0.520171577475196 1104 52-53 0.5066875099021786 1104 54-55 0.582496850862043 1104 56-57 0.7354701776479118 1104 58-59 0.7633658285169531 1104 60-61 0.7469392432132267 1104 62-63 0.7540933231006548 1104 64-65 0.5391038576853404 1104 66-67 0.5066976637888416 1104 68-69 0.5473585386601059 1104 70-71 0.5246199437824899 1104 72-73 0.6061285347187919 1104 74-75 0.6042939143999675 1104 76-77 0.6126821459418501 1104 78-79 0.7105035588467992 1104 80-81 0.7522816246612258 1104 82-83 0.868507687706181 1104 84-85 0.8322316743783915 1104 86-87 0.9292258451874211 1104 88-89 1.2035859944415925 1104 90-91 1.277034267620234 1104 92-93 1.2314987973644982 1104 94-95 1.098892192189588 1104 96-97 0.9514959633320323 1104 98-99 0.996013917927467 1104 100-101 0.7102227404150732 1105 1 0.12302709779166321 1105 2 0.08682717446881583 1105 3 0.11192601790133239 1105 4 0.1497785619155536 1105 5 0.16784037476094937 1105 6 0.1112169595409398 1105 7 0.09500397841368624 1105 8 0.15621837145496897 1105 9 0.13881064033185453 1105 10-11 0.16144973720822264 1105 12-13 0.1842323733485216 1105 14-15 0.18655250643538324 1105 16-17 0.2039430955914412 1105 18-19 0.1886221595089026 1105 20-21 0.20350845332462342 1105 22-23 0.20549716442688037 1105 24-25 0.23979685415469731 1105 26-27 0.2442399050426971 1105 28-29 0.18895235687877943 1105 30-31 0.30399381813305837 1105 32-33 0.3325513372457962 1105 34-35 0.3369620214155731 1105 36-37 0.30792977674470734 1105 38-39 0.4357979710305244 1105 40-41 0.45704864036977 1105 42-43 0.41787668732505523 1105 44-45 0.38037645527557373 1105 46-47 0.3169294854843727 1105 48-49 0.34036186438800087 1105 50-51 0.4080030000573345 1105 52-53 0.48450127311469515 1105 54-55 0.5147297667662372 1105 56-57 0.48159063577298156 1105 58-59 0.5375332320575836 1105 60-61 0.3756626616939158 1105 62-63 0.3649987591252071 1105 64-65 0.4292154642185366 1105 66-67 0.434434297065728 1105 68-69 0.5998403245012014 1105 70-71 0.45733438833315176 1105 72-73 0.36930115270966724 1105 74-75 0.46394325058748365 1105 76-77 0.5624245672357411 1105 78-79 0.6026632624812116 1105 80-81 0.7744437494529599 1105 82-83 0.674707984276619 1105 84-85 0.5231388629680538 1105 86-87 0.6249385047391307 1105 88-89 0.8321993085877502 1105 90-91 0.8822763008461365 1105 92-93 0.8018859094103128 1105 94-95 0.8826555298929541 1105 96-97 0.6803606254738916 1105 98-99 0.7995097589993954 1105 100-101 0.6237057701221893 1106 1 0.021519901905968197 1106 2 0.04623283993271343 1106 3 -0.002577735572188544 1106 4 0.05802376120218611 1106 5 0.027985121971767057 1106 6 0.06593389372036285 1106 7 0.06503218130863075 1106 8 0.03483933284189078 1106 9 0.18160604491897203 1106 10-11 0.11917442718375781 1106 12-13 0.11959009389101283 1106 14-15 0.12628997066332914 1106 16-17 0.10396943211833332 1106 18-19 0.05128360034503743 1106 20-21 0.13087293426693236 1106 22-23 0.18013591852252375 1106 24-25 0.20767002407713875 1106 26-27 0.16955615073968033 1106 28-29 0.028019380229117985 1106 30-31 0.0690222284022397 1106 32-33 0.11883767957916547 1106 34-35 0.2308714403529919 1106 36-37 0.2944357283850323 1106 38-39 0.2533331209620968 1106 40-41 0.08710255766892772 1106 42-43 0.17307576658348722 1106 44-45 0.18945986118988145 1106 46-47 0.2388715451251997 1106 48-49 0.0975077728944953 1106 50-51 0.09029635163960847 1106 52-53 0.11094874522286347 1106 54-55 0.0673308617021533 1106 56-57 0.037814351089643594 1106 58-59 0.035723269799689206 1106 60-61 0.05552579470374752 1106 62-63 0.12205487459117137 1106 64-65 0.06404852796537597 1106 66-67 0.05745449531548985 1106 68-69 0.08782319539000838 1106 70-71 0.07385153818177059 1106 72-73 0.29506117925356534 1106 74-75 0.09901811268362337 1106 76-77 0.2324757886699409 1106 78-79 0.49475463156585775 1106 80-81 0.4264082055103593 1106 82-83 0.3313097428100704 1106 84-85 0.32520451764849057 1106 86-87 0.43812066226059443 1106 88-89 0.48707882416155357 1106 90-91 0.5202645634648597 1106 92-93 0.46234793918925376 1106 94-95 0.5133558326593253 1106 96-97 0.5196559678487418 1106 98-99 0.6847275019383083 1106 100-101 0.7096287928089318 1107 1 0.016572041424133488 1107 2 -0.026161982073759305 1107 3 -0.010920058477616124 1107 4 -0.030325753360919805 1107 5 0.058747437685539694 1107 6 0.03365054240788368 1107 7 0.05321988357400187 1107 8 0.06665163057651569 1107 9 0.02136152855795359 1107 10-11 0.048084891045675704 1107 12-13 0.03091335890359659 1107 14-15 0.07964847479853887 1107 16-17 -0.024935638000329163 1107 18-19 0.025850302969992356 1107 20-21 0.09270860488901178 1107 22-23 -0.013343045878769999 1107 24-25 -0.02972300001491135 1107 26-27 0.016445758794340293 1107 28-29 -0.058242755693214576 1107 30-31 0.06592981560108768 1107 32-33 0.14215662959714592 1107 34-35 0.12423713614587228 1107 36-37 0.07911929544724217 1107 38-39 0.05524250607536629 1107 40-41 0.011471489707766125 1107 42-43 0.03914732357737449 1107 44-45 0.004990245943567118 1107 46-47 0.024885568857676788 1107 48-49 0.13105865387255733 1107 50-51 0.08219674718077385 1107 52-53 0.05502461721135887 1107 54-55 0.05445240071689739 1107 56-57 0.014689575828946033 1107 58-59 0.02581316552065971 1107 60-61 -0.1258046619665194 1107 62-63 -0.05413354683996374 1107 64-65 0.07369973256803775 1107 66-67 -0.030082361930105606 1107 68-69 0.04679299042776108 1107 70-71 -0.09317291345433887 1107 72-73 0.1641730095304439 1107 74-75 0.18564522523306692 1107 76-77 0.32490477105038096 1107 78-79 0.46445437985784466 1107 80-81 0.3377476517527178 1107 82-83 0.3427894263771343 1107 84-85 0.3031063515211976 1107 86-87 0.41543637603261985 1107 88-89 0.6424905465635682 1107 90-91 0.6819024635008155 1107 92-93 0.4066485504801527 1107 94-95 0.5668024346010725 1107 96-97 0.4920579815128896 1107 98-99 0.4501230431105476 1107 100-101 0.38744612470393136 1108 1 -0.03912054913784857 1108 2 -0.011467642029437286 1108 3 -0.02913631800590366 1108 4 -0.0708446020751623 1108 5 0.012543496551188582 1108 6 0.02636025484121518 1108 7 -0.02493560127577865 1108 8 -0.01898206835586791 1108 9 -0.015217421830833189 1108 10-11 0.028720135642124944 1108 12-13 0.007337472978271364 1108 14-15 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22.0 5 54.0 6 101.0 7 257.0 8 563.0 9 1168.0 10 1992.0 11 3069.0 12 4299.0 13 6278.0 14 7519.0 15 9528.0 16 12470.0 17 16389.0 18 16053.0 19 12967.0 20 12928.0 21 14035.0 22 15235.0 23 17192.0 24 19958.0 25 23977.0 26 29519.0 27 35700.0 28 43618.0 29 52853.0 30 65529.0 31 80277.0 32 99524.0 33 128044.0 34 174017.0 35 246190.0 36 396165.0 37 667198.0 38 585031.0 39 48360.0 40 2.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.2707933418492 19.184152598349126 15.045060397410312 23.499993662391365 2 15.405760407738923 20.434381272828837 39.71399148273391 24.44586683669833 3 19.925796066636774 23.64154029866258 29.49260506225736 26.94005857244328 4 13.41843343116474 16.74468952426831 36.7295425682482 33.10733447631875 5 14.344525484911506 37.06847063144268 33.93658994058138 14.65041394306444 6 32.55777454208898 35.71577793874296 17.217672010468746 14.508775508699317 7 29.209573971673013 32.0326168666306 20.357979380618005 18.399829781078385 8 25.89673034310278 35.397811240277285 19.515416785313143 19.190041631306798 9 25.812147549749888 17.787666641971814 20.44484439961139 35.95534140866691 10-11 25.26802984589359 26.379543955139166 27.055872745907177 21.29655345306006 12-13 26.187661445519588 23.444707114961325 27.48552805610202 22.882103383417068 14-15 22.685138369231804 25.300119521128977 25.879677615981844 26.135064493657374 16-17 22.58381084800989 28.54075377096149 26.412091654728798 22.463343726299822 18-19 22.529973522671455 27.602097119585462 27.927770718483252 21.94015863925983 20-21 23.28653132249358 26.455682513622254 27.754514681206434 22.503271482677732 22-23 22.854084674784602 26.441550270233073 27.790170067943613 22.914194987038712 24-25 23.124346163494984 26.581290256095873 27.76975194107918 22.524611639329958 26-27 22.861448543412518 26.51902469785584 28.039251968268935 22.580274790462703 28-29 22.40083466258347 27.320294919730614 27.867748184138392 22.411122233547523 30-31 22.780526786833455 26.589052268936065 27.694035701733473 22.936385242497007 32-33 22.617470542611436 26.736168044838358 27.782796723566648 22.863564688983555 34-35 23.32533648642599 26.610188386752448 27.487383679234267 22.577091447587296 36-37 23.130785571729238 26.34371708727642 27.86970854713251 22.655788793861827 38-39 22.795093987889643 26.858219943969114 27.816296501256105 22.530389566885137 40-41 23.018786226886828 26.687553323571866 27.639814207901658 22.653846241639645 42-43 22.721638682755923 26.77424361276513 27.25192120918023 23.252196495298715 44-45 23.027958976624106 26.58157669885732 27.240195029394325 23.150269295124247 46-47 23.212603512172173 26.957439973034607 27.093408049942152 22.736548464851065 48-49 23.16344913814382 26.799807029044302 27.189189834475442 22.847553998336434 50-51 22.713166019275327 26.99309398521188 27.481788717167987 22.811951278344804 52-53 23.219139520026797 27.156365663874098 26.78467881969589 22.83981599640321 54-55 22.79177991479925 26.65297214245161 27.13285397231906 23.42239397043008 56-57 23.06559837421239 26.887308769207785 27.000050735640453 23.04704212093937 58-59 23.465485380868465 26.5586712185003 26.986239516193876 22.989603884437358 60-61 23.008859811469616 26.73120603757897 27.57779151827457 22.682142632676843 62-63 22.831149711285775 26.88154053015414 27.372892796970287 22.914416961589797 64-65 22.64772278607835 27.004931188251525 27.115338261888244 23.23200776378188 66-67 22.696382883026807 26.87166202555601 26.896906952256188 23.535048139160992 68-69 22.58095663276726 26.78952355153913 27.237245677663598 23.39227413803001 70-71 22.92174388723418 26.64036723468337 27.108662380529875 23.329226497552575 72-73 22.899553631775884 26.375839727368838 27.405948603576352 23.31865803727893 74-75 22.560151567871458 27.169773360949435 26.903929184996155 23.36614588618295 76-77 22.69199819033731 26.60726782638289 27.00923325661682 23.69150072666298 78-79 22.666310171317903 26.718875796716198 27.141947763717315 23.472866268248588 80-81 22.816534319453496 26.755385412687353 26.994337205905484 23.433743061953663 82-83 22.782668567819144 26.55736444369697 27.261062386568994 23.398904601914893 84-85 22.607323416302183 26.72442336058077 27.087595682309978 23.580657540807074 86-87 22.583435498293188 26.69607306950404 27.141492376994325 23.578999055208445 88-89 22.425003902697213 26.648162772250572 26.891927178312912 24.034906146739303 90-91 22.578327361247 26.457672716983016 27.074615047543233 23.889384874226753 92-93 22.70203578038248 26.50861259289975 27.117159282145238 23.672192344572533 94-95 22.386688793725913 26.313574698048324 27.271653190121892 24.028083318103864 96-97 22.088854417528616 26.237115519590798 27.595145109172897 24.078884953707686 98-99 22.115907569159337 26.452978954017365 26.967796391766154 24.463317085057145 100-101 22.019497631657995 25.773101582657425 27.67874745509226 24.528653330592316 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 73.0 1 53.0 2 43.0 3 62.5 4 52.5 5 52.5 6 121.5 7 253.5 8 393.5 9 517.0 10 596.0 11 653.0 12 739.5 13 902.0 14 1050.0 15 1166.0 16 1295.5 17 1421.0 18 1637.0 19 1813.5 20 2040.5 21 2465.0 22 3025.0 23 3756.5 24 4804.0 25 6310.5 26 8594.5 27 12051.0 28 15775.0 29 20411.5 30 26297.5 31 31909.0 32 37711.0 33 43362.0 34 49422.0 35 55624.5 36 62361.0 37 69597.5 38 75677.5 39 79749.5 40 83437.5 41 90020.0 42 96098.5 43 100436.5 44 105482.0 45 110656.0 46 113207.5 47 121778.5 48 141928.5 49 141436.5 50 122869.5 51 112598.5 52 105472.0 53 101960.5 54 99659.0 55 92278.0 56 81814.0 57 72921.0 58 65178.5 59 56112.5 60 46388.0 61 37750.5 62 30464.0 63 23695.0 64 17753.5 65 13178.5 66 9718.0 67 7840.5 68 6085.5 69 4143.0 70 3064.5 71 2321.5 72 1744.5 73 1407.0 74 1120.5 75 870.0 76 583.0 77 330.0 78 191.5 79 117.0 80 76.0 81 42.5 82 27.0 83 17.5 84 9.0 85 5.5 86 2.5 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2776941937019701 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0022822256404890703 16-17 5.968897828971414E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.2288907294941148E-4 26-27 0.0012640018931939468 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0034935607881332697 36-37 0.021207142874698437 38-39 0.007145121812915782 40-41 0.017748693250265003 42-43 0.005301785718674609 44-45 4.7400070994773003E-4 46-47 7.02223273996637E-5 48-49 0.0 50-51 0.0 52-53 1.7555581849915925E-5 54-55 0.0 56-57 1.7555581849915925E-5 58-59 4.915562917976459E-4 60-61 0.0023875591315885656 62-63 0.0013517798024435263 64-65 1.7555581849915925E-5 66-67 0.0 68-69 4.7400070994773003E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.2346654625878262 78-79 0.0 80-81 0.0011586684020944511 82-83 0.0 84-85 0.0 86-87 0.10621127019199135 88-89 0.36220676472746544 90-91 0.36204876449081613 92-93 0.0 94-95 1.7555581849915925E-5 96-97 0.0 98-99 0.0 100-101 2.633337277487389E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2848097.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.45270753849398 #Duplication Level Percentage of deduplicated Percentage of total 1 72.73645103546609 38.15223793551291 2 14.8981399065507 15.628955507717398 3 5.2382542256666635 8.242818507335215 4 2.4235662283844808 5.084904423104883 5 1.2960125985947142 3.3989684900146075 6 0.8184204704043532 2.575702174658173 7 0.5130342159192606 1.8837023579397483 8 0.357007612166553 1.4980812695990635 9 0.26459348162277213 1.2490780057336035 >10 1.3262550387628584 12.354554121625611 >50 0.08405663625896004 3.021746291931649 >100 0.0406605181248332 3.9583455155049947 >500 0.0022091520484932695 0.8687891357663529 >1k 0.0012049920264007171 1.4876021233395833 >5k 6.694400146670652E-5 0.1951632857670578 >10k+ 6.694400146670652E-5 0.3993508544492171 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11373 0.3993192647581877 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5558 0.19514784784366546 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4188 0.1470455535748958 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3814 0.13391397835115867 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3768 0.13229886482096642 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3244 0.11390061504225452 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3225 0.11323350293195773 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.02223273996637E-5 2 0.0 0.0 0.0 0.0 7.02223273996637E-5 3 0.0 0.0 0.0 0.0 7.02223273996637E-5 4 0.0 0.0 0.0 0.0 7.02223273996637E-5 5 0.0 0.0 0.0 0.0 7.02223273996637E-5 6 0.0 0.0 0.0 0.0 1.0533349109949556E-4 7 0.0 0.0 0.0 0.0 1.0533349109949556E-4 8 0.0 0.0 0.0 0.0 1.0533349109949556E-4 9 0.0 0.0 0.0 0.0 1.0533349109949556E-4 10-11 0.0 0.0 0.0 1.7555581849915925E-5 1.0533349109949556E-4 12-13 0.0 0.0 0.0 5.266674554974778E-5 2.4577814589882296E-4 14-15 0.0 0.0 0.0 1.404446547993274E-4 2.984448914485707E-4 16-17 0.0 0.0 0.0 1.5800023664924332E-4 3.1600047329848665E-4 18-19 0.0 0.0 0.0 2.2822256404890704E-4 3.1600047329848665E-4 20-21 0.0 7.02223273996637E-5 0.0 2.633337277487389E-4 4.564451280978141E-4 22-23 0.0 7.02223273996637E-5 0.0 3.335560551484026E-4 4.7400070994773E-4 24-25 0.0 7.02223273996637E-5 0.0 5.793342010472256E-4 4.915562917976459E-4 26-27 0.0 7.02223273996637E-5 0.0 9.128902561956283E-4 4.915562917976459E-4 28-29 0.0 7.02223273996637E-5 0.0 0.0019837807490405 4.915562917976459E-4 30-31 0.0 1.0533349109949556E-4 0.0 0.005582675028273264 5.091118736475619E-4 32-33 0.0 1.4044465479932743E-4 0.0 0.014904688990578622 5.617786191973096E-4 34-35 0.0 1.7555581849915927E-4 0.0 0.028299597942064474 5.617786191973096E-4 36-37 3.511116369983185E-5 1.7555581849915927E-4 0.0 0.05087607620105636 5.617786191973096E-4 38-39 3.511116369983185E-5 1.7555581849915927E-4 0.0 0.08409123706109728 5.617786191973096E-4 40-41 3.511116369983185E-5 1.7555581849915927E-4 0.0 0.1201504021808246 5.617786191973096E-4 42-43 3.511116369983185E-5 2.2822256404890704E-4 0.0 0.1613709083644272 5.617786191973096E-4 44-45 3.511116369983185E-5 2.808893095986548E-4 0.0 0.21373920902272642 5.617786191973096E-4 46-47 3.511116369983185E-5 2.808893095986548E-4 0.0 0.2789055288496143 5.617786191973096E-4 48-49 3.511116369983185E-5 2.984448914485707E-4 0.0 0.3480745213382831 5.617786191973096E-4 50-51 3.511116369983185E-5 3.1600047329848665E-4 0.0 0.42445885796726723 5.617786191973096E-4 52-53 3.511116369983185E-5 3.1600047329848665E-4 0.0 0.5012996397243492 6.320009465969733E-4 54-55 3.511116369983185E-5 3.5111163699831855E-4 0.0 0.5910788853048193 6.320009465969733E-4 56-57 3.511116369983185E-5 3.5111163699831855E-4 0.0 0.7002043820838968 6.495565284468892E-4 58-59 3.511116369983185E-5 3.5111163699831855E-4 0.0 0.8233392331792071 6.671121102968052E-4 60-61 3.511116369983185E-5 3.5111163699831855E-4 0.0 0.9551640972902258 6.671121102968052E-4 62-63 3.511116369983185E-5 3.5111163699831855E-4 0.0 1.095801863489902 6.671121102968052E-4 64-65 3.511116369983185E-5 3.5111163699831855E-4 0.0 1.2480087581286732 7.373344376964689E-4 66-67 3.511116369983185E-5 3.686672188482345E-4 0.0 1.426794803688217 7.724456013963007E-4 68-69 3.511116369983185E-5 3.862228006981504E-4 0.0 1.6306677757112906 8.075567650961326E-4 70-71 3.511116369983185E-5 3.862228006981504E-4 0.0 1.8500949932533899 8.251123469460485E-4 72-73 3.511116369983185E-5 3.862228006981504E-4 0.0 2.0777382231012496 8.777790924957964E-4 74-75 3.511116369983185E-5 3.862228006981504E-4 0.0 2.317196359534103 9.128902561956282E-4 76-77 3.511116369983185E-5 3.862228006981504E-4 0.0 2.5852174276367696 9.480014198954601E-4 78-79 3.511116369983185E-5 3.862228006981504E-4 0.0 2.8964427826720787 9.480014198954601E-4 80-81 3.511116369983185E-5 3.862228006981504E-4 0.0 3.239496407601286 9.480014198954601E-4 82-83 3.511116369983185E-5 3.862228006981504E-4 0.0 3.5860260377367768 9.831125835952918E-4 84-85 3.511116369983185E-5 3.862228006981504E-4 0.0 3.958643262501242 9.831125835952918E-4 86-87 3.511116369983185E-5 3.862228006981504E-4 0.0 4.344953841108643 0.0010182237472951237 88-89 3.511116369983185E-5 3.862228006981504E-4 0.0 4.779349158402962 0.0010533349109949556 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9410 0.0 22.650232 1 GCGATAT 150 3.059458E-5 22.143864 9 TACATGG 9535 0.0 22.044058 2 ACATGGG 9595 0.0 21.164465 3 GAGTACT 5980 0.0 19.281984 12-13 CATGGGG 5350 0.0 18.97879 4 GTACTTT 6120 0.0 18.802126 14-15 CCGTCCG 130 0.0076108375 18.250437 9 AGTACTT 6325 0.0 17.517534 12-13 CCTATAC 600 0.0 17.397223 3 AGAGTAC 11995 0.0 17.366444 10-11 GTGTAGC 1590 0.0 16.756186 1 GTATAGG 810 0.0 15.858532 1 GTATAGC 1060 0.0 15.708924 1 TCGCGCT 315 0.0 15.063721 60-61 TAGCCCT 1890 0.0 15.062531 4 ACTTTTT 7685 0.0 14.786662 16-17 GTGTAAG 1335 0.0 14.611232 1 CGCGGAA 295 1.8189894E-12 14.554044 82-83 ATGGGGA 3415 0.0 14.449444 5 >>END_MODULE