FastQCFastQC Report
Fri 27 May 2016
SRR939251_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939251_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2144770
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67710.3156981867519594No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50480.23536323242119203No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT38420.17913342689425904No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35380.16495941289742022No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG22820.10639835506837562No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG22260.10378735249001059No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG103850.028.1967491
TACATGG105200.027.4541052
GTATCAA118000.026.947171
ACATGGG108200.025.9039483
CATGGGG55950.024.0720924
GGTATCA86550.023.5239681
TATCAAC143350.021.9341892
AGGTACG1302.9748087E-421.8878695
GAGTACT61050.021.81155612-13
TCAACGC144250.021.6981984
ATCAACG145100.021.6042793
CAACGCA145000.021.5532635
CATGGGA53350.021.2451154
AACGCAG147300.021.2330446
AGTACTT64400.020.41940512-13
ATGGGGA33350.019.7658065
GTATATA8000.019.5769181
GTACTTT68200.019.31934214-15
AGAGTAC124050.019.15688710-11
ACGCAGA163050.019.1529487
Produced by FastQC (version 0.11.5)