##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939251_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2144770 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.930121644745125 33.0 31.0 34.0 30.0 34.0 2 31.98844258358705 34.0 31.0 34.0 30.0 34.0 3 32.041953682679264 34.0 31.0 34.0 30.0 34.0 4 35.526702163868386 37.0 35.0 37.0 33.0 37.0 5 35.55518493824513 37.0 35.0 37.0 33.0 37.0 6 35.64865183679369 37.0 35.0 37.0 33.0 37.0 7 35.57911477687584 37.0 35.0 37.0 33.0 37.0 8 35.56101167024902 37.0 35.0 37.0 33.0 37.0 9 37.19326221459644 39.0 37.0 39.0 34.0 39.0 10-11 37.088032050056654 39.0 37.0 39.0 33.0 39.0 12-13 36.98607985937886 39.0 37.0 39.0 33.0 39.0 14-15 38.23003702028656 40.0 38.0 41.0 33.0 41.0 16-17 38.15392979200567 40.0 38.0 41.0 33.0 41.0 18-19 38.06489880033756 40.0 38.0 41.0 33.0 41.0 20-21 38.03137236160521 40.0 38.0 41.0 33.0 41.0 22-23 38.03342689425906 40.0 38.0 41.0 33.0 41.0 24-25 37.9398079980604 40.0 38.0 41.0 33.0 41.0 26-27 37.849101768488 40.0 38.0 41.0 33.0 41.0 28-29 37.70567077122489 40.0 38.0 41.0 32.0 41.0 30-31 37.52491455960313 40.0 37.0 41.0 32.0 41.0 32-33 37.314829795269425 40.0 37.0 41.0 31.5 41.0 34-35 37.01648405190301 40.0 36.5 41.0 30.5 41.0 36-37 36.77722366500837 40.0 36.0 41.0 30.0 41.0 38-39 36.17842519244488 38.5 35.5 40.5 29.0 41.0 40-41 36.146032441707035 39.0 35.0 40.0 30.0 41.0 42-43 36.33403581736037 39.0 35.0 41.0 30.0 41.0 44-45 36.239193946204026 39.0 35.0 41.0 29.5 41.0 46-47 35.948223585745794 39.0 35.0 41.0 28.5 41.0 48-49 35.69226397236067 39.0 35.0 41.0 27.0 41.0 50-51 34.52538547256815 38.0 34.0 40.0 25.5 40.5 52-53 34.61108370594516 38.0 34.0 39.5 26.0 40.5 54-55 35.07397762930291 39.0 35.0 40.0 26.0 41.0 56-57 34.968245779267704 38.5 35.0 40.0 26.0 41.0 58-59 34.73520633914126 38.0 34.0 40.0 26.0 41.0 60-61 34.40928957417346 38.0 34.0 40.0 25.0 41.0 62-63 34.014630240072364 37.0 34.0 40.0 23.5 41.0 64-65 33.60176988674776 36.5 33.0 39.5 23.0 41.0 66-67 33.22109736708365 36.0 33.0 39.0 22.0 41.0 68-69 32.81217519827301 35.5 33.0 39.0 21.5 40.5 70-71 32.388223212745416 35.0 32.0 38.0 20.0 40.0 72-73 31.95029653529283 35.0 32.0 37.0 19.0 39.5 74-75 31.496061582360813 35.0 31.5 37.0 17.5 39.0 76-77 31.05409274654159 35.0 31.0 36.0 14.5 39.0 78-79 30.675277069336104 35.0 31.0 36.0 9.5 37.5 80-81 30.28552525445619 34.0 30.5 35.0 7.0 37.0 82-83 29.88861742750971 34.0 30.0 35.0 3.5 37.0 84-85 29.49725238603673 34.0 30.0 35.0 2.0 36.0 86-87 29.08771080348942 34.0 29.0 35.0 2.0 36.0 88-89 28.68441138210624 34.0 29.0 35.0 2.0 35.5 90-91 28.29683276062235 34.0 29.0 35.0 2.0 35.0 92-93 27.853273078232164 34.0 27.5 35.0 2.0 35.0 94-95 27.371603015707976 33.5 27.0 35.0 2.0 35.0 96-97 26.76824834364524 33.0 25.5 35.0 2.0 35.0 98-99 25.96569795362673 33.0 24.5 35.0 2.0 35.0 100-101 23.241689085542973 29.5 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31560968109926435 1101 2 0.4051970747613325 1101 3 0.4495437146887298 1101 4 0.30752622711924715 1101 5 0.2729217959534722 1101 6 0.34179239965160946 1101 7 0.34749520984784255 1101 8 0.27683968982051965 1101 9 0.23581867835797965 1101 10-11 0.42316497365493433 1101 12-13 0.4498124367712677 1101 14-15 0.6307492991775234 1101 16-17 0.6696089968720997 1101 18-19 0.6104423469339508 1101 20-21 0.6762796022654456 1101 22-23 0.6752210975386816 1101 24-25 0.6644962854878074 1101 26-27 0.5687106555533035 1101 28-29 0.6707099989998611 1101 30-31 0.6380983916040606 1101 32-33 0.7100609958619657 1101 34-35 0.6680399163961752 1101 36-37 0.7304113626770317 1101 38-39 0.9189116401029551 1101 40-41 0.8753212990904231 1101 42-43 0.7186921360536189 1101 44-45 0.7446626175130362 1101 46-47 0.7854401369092656 1101 48-49 0.8155910701885034 1101 50-51 0.9416001388130155 1101 52-53 0.8724392407071662 1101 54-55 0.8819444008104469 1101 56-57 0.8701036692977411 1101 58-59 0.8883808121672772 1101 60-61 0.8548531719625103 1101 62-63 1.037088369272972 1101 64-65 0.8135386933041104 1101 66-67 0.7702437257689283 1101 68-69 0.8905884748320361 1101 70-71 0.9592487982815712 1101 72-73 0.9227615805434084 1101 74-75 0.8814889937605379 1101 76-77 0.997804980009029 1101 78-79 0.9650912982232285 1101 80-81 0.8901524823688582 1101 82-83 0.8792917602981518 1101 84-85 1.0583822963620086 1101 86-87 1.000266649151282 1101 88-89 0.7857367064250944 1101 90-91 0.8802062283652354 1101 92-93 0.90377300542524 1101 94-95 1.0276764062506096 1101 96-97 1.024082426788123 1101 98-99 1.09172573181554 1101 100-101 0.8045187815626385 1102 1 0.33004247634186257 1102 2 0.3136634488057979 1102 3 0.3671153276406258 1102 4 0.30983945143329805 1102 5 0.32325362306386296 1102 6 0.2967954040139986 1102 7 0.3419601773656993 1102 8 0.28423230744197525 1102 9 0.39348788593382267 1102 10-11 0.4788530534766622 1102 12-13 0.42995737367965603 1102 14-15 0.491482148040582 1102 16-17 0.4844514901425967 1102 18-19 0.40529168422280293 1102 20-21 0.4451595274662594 1102 22-23 0.5515993993059993 1102 24-25 0.43579721481927436 1102 26-27 0.47336755197959235 1102 28-29 0.4406035933759114 1102 30-31 0.5124792639200138 1102 32-33 0.43466106537994875 1102 34-35 0.34203776618883097 1102 36-37 0.3332603874044153 1102 38-39 0.7761281724490203 1102 40-41 0.525095769824155 1102 42-43 0.5112340421935642 1102 44-45 0.5716591712534083 1102 46-47 0.6321719834200294 1102 48-49 0.6291973289624622 1102 50-51 0.6567146413956806 1102 52-53 0.6766537009862645 1102 54-55 0.5807825343873674 1102 56-57 0.5568521408452085 1102 58-59 0.7854421954673327 1102 60-61 0.6621516169692043 1102 62-63 0.6063930816760106 1102 64-65 0.5579718657760679 1102 66-67 0.5489868514374976 1102 68-69 0.4168999531130524 1102 70-71 0.5436154706066745 1102 72-73 0.4736575002689989 1102 74-75 0.36627856714107665 1102 76-77 0.45087037271826347 1102 78-79 0.3761093594259961 1102 80-81 0.3271826634120032 1102 82-83 0.19101645292358782 1102 84-85 0.23981614515231442 1102 86-87 0.18179736505177146 1102 88-89 0.12269278884431856 1102 90-91 0.1819928135503588 1102 92-93 0.24092403458776346 1102 94-95 0.07344416230177231 1102 96-97 0.021450982565525578 1102 98-99 -0.019267540029410668 1102 100-101 -0.06306158849936239 1103 1 0.31541013731428436 1103 2 0.34301558378840724 1103 3 0.3538361857997998 1103 4 0.3356956945063061 1103 5 0.2430961687919151 1103 6 0.2034852229145514 1103 7 0.2759858515393532 1103 8 0.2494276305635239 1103 9 0.3844981088535846 1103 10-11 0.4291731458311787 1103 12-13 0.37683881865891067 1103 14-15 0.5031775244313437 1103 16-17 0.5326819979811219 1103 18-19 0.583285215590422 1103 20-21 0.6346627880195257 1103 22-23 0.5831502809513367 1103 24-25 0.5268597528829133 1103 26-27 0.5269563867511948 1103 28-29 0.4988340058068843 1103 30-31 0.3668137882576801 1103 32-33 0.41899554201383893 1103 34-35 0.4234752718536541 1103 36-37 0.35396342979240103 1103 38-39 0.8255492870955052 1103 40-41 0.6386899951227392 1103 42-43 0.49457429257480356 1103 44-45 0.5078438389409357 1103 46-47 0.5077871633984401 1103 48-49 0.14881511038692707 1103 50-51 0.1675115966225036 1103 52-53 0.304590464857327 1103 54-55 0.33879319141151143 1103 56-57 0.32493704085014485 1103 58-59 0.3501873539313394 1103 60-61 0.42599763871258034 1103 62-63 0.320679647814913 1103 64-65 0.23951773852464697 1103 66-67 0.2310984114941732 1103 68-69 0.31792892361780645 1103 70-71 0.25312529838013376 1103 72-73 0.23537990880097936 1103 74-75 0.16603400576937233 1103 76-77 0.1665105335754049 1103 78-79 0.19940263748654985 1103 80-81 0.33077157768990517 1103 82-83 0.3303071136368203 1103 84-85 0.28749289162740155 1103 86-87 0.3141566667320568 1103 88-89 0.32087813710575475 1103 90-91 0.2606599222720405 1103 92-93 0.17085632702464792 1103 94-95 0.324316346387473 1103 96-97 0.5355450639577093 1103 98-99 0.6109669558450079 1103 100-101 0.4132956237554204 1104 1 0.35427351790600525 1104 2 0.4073047600374835 1104 3 0.327168733732492 1104 4 0.2884391196178413 1104 5 0.28612372291996735 1104 6 0.296697811233372 1104 7 0.349024752639842 1104 8 0.3887910323403858 1104 9 0.49078529266523674 1104 10-11 0.3485524158012865 1104 12-13 0.4607481555224169 1104 14-15 0.4485324996860598 1104 16-17 0.47999362842118387 1104 18-19 0.4481974677371028 1104 20-21 0.5099450413753033 1104 22-23 0.5647046043235164 1104 24-25 0.5597597724145871 1104 26-27 0.5653848259017593 1104 28-29 0.5660915572790373 1104 30-31 0.4898974365263342 1104 32-33 0.5008616746080747 1104 34-35 0.47653593165219377 1104 36-37 0.46121931745530986 1104 38-39 1.0420931574051977 1104 40-41 0.7561430441823802 1104 42-43 0.6671234550380234 1104 44-45 0.7577522519252611 1104 46-47 0.6801899674854113 1104 48-49 0.7560300487071387 1104 50-51 0.5515885286822595 1104 52-53 0.5727046714115005 1104 54-55 0.5884863942856597 1104 56-57 0.6569563782406931 1104 58-59 0.7080020644853633 1104 60-61 0.7970113429203636 1104 62-63 0.6669395389542458 1104 64-65 0.5456474030035636 1104 66-67 0.497179360464834 1104 68-69 0.5583972859231281 1104 70-71 0.7019701393830005 1104 72-73 0.6007632721552199 1104 74-75 0.5487451416137112 1104 76-77 0.5724369756381549 1104 78-79 0.6565196332464218 1104 80-81 0.5990013983188653 1104 82-83 0.5014035432675072 1104 84-85 0.4520524110448818 1104 86-87 0.45027925743807984 1104 88-89 0.42645109720843877 1104 90-91 0.4213871257600452 1104 92-93 0.511989799663251 1104 94-95 0.5286703595264015 1104 96-97 0.5029823274978646 1104 98-99 0.5316115217509747 1104 100-101 0.4618307998866804 1105 1 0.20258286216050791 1105 2 0.17892859755075108 1105 3 0.27643717905007037 1105 4 0.23356855766343898 1105 5 0.2424215492480144 1105 6 0.2625410993082795 1105 7 0.2414303674309508 1105 8 0.26056201255237 1105 9 0.33961151773217324 1105 10-11 0.4273811955664897 1105 12-13 0.44326382037769463 1105 14-15 0.46624507434661666 1105 16-17 0.3736439159732683 1105 18-19 0.39006421942834635 1105 20-21 0.4439843163955004 1105 22-23 0.4146997523771887 1105 24-25 0.3764185835491176 1105 26-27 0.36729916627470516 1105 28-29 0.3384991326525437 1105 30-31 0.3747439169838884 1105 32-33 0.3994153985263864 1105 34-35 0.15899584863440452 1105 36-37 0.2328956352043221 1105 38-39 0.6217621160388873 1105 40-41 0.5383168943701193 1105 42-43 0.2516904039757435 1105 44-45 0.09531651545921704 1105 46-47 0.1709044314344581 1105 48-49 0.13977295892435393 1105 50-51 0.08773701408284751 1105 52-53 0.09601233233768625 1105 54-55 0.15520808268376385 1105 56-57 0.21402417874450208 1105 58-59 0.3375544773879753 1105 60-61 0.3177766829922959 1105 62-63 0.32887770337384126 1105 64-65 0.4433153991695349 1105 66-67 0.3394064434350952 1105 68-69 0.2646154017044253 1105 70-71 0.2672085184764583 1105 72-73 0.3127274786540539 1105 74-75 0.4977932689604678 1105 76-77 0.45650417410454125 1105 78-79 0.3993753898331889 1105 80-81 0.38743178378124554 1105 82-83 0.21716464531284885 1105 84-85 0.40138125814322123 1105 86-87 0.44090905987474827 1105 88-89 0.3798278538110438 1105 90-91 0.4862358638579245 1105 92-93 0.2411071217057419 1105 94-95 0.38258815765374976 1105 96-97 0.5183872570753287 1105 98-99 0.4531327752562575 1105 100-101 -0.017789045167898365 1106 1 0.1416203476999307 1106 2 0.15703155683723935 1106 3 0.18196671593852898 1106 4 0.2589622584508362 1106 5 0.2155943857595659 1106 6 0.3277018449141309 1106 7 0.11516996514711053 1106 8 0.1503971620093978 1106 9 0.2308479434514723 1106 10-11 0.31753097912434924 1106 12-13 0.36671070808900197 1106 14-15 0.37745341856425085 1106 16-17 0.36976614980541456 1106 18-19 0.3287611817748797 1106 20-21 0.3893727927174808 1106 22-23 0.4914301233142382 1106 24-25 0.3731267038610824 1106 26-27 0.5387990076748679 1106 28-29 0.48796712347240856 1106 30-31 0.5247205934775252 1106 32-33 0.37788733568352484 1106 34-35 0.2921492799886636 1106 36-37 0.2783955978867141 1106 38-39 0.7378627989511557 1106 40-41 0.5923884788758471 1106 42-43 0.40901794996970153 1106 44-45 0.3936584473918998 1106 46-47 0.2366750867317009 1106 48-49 0.18315631340432503 1106 50-51 0.04922267479113884 1106 52-53 0.13160237040165157 1106 54-55 0.1662823167398173 1106 56-57 0.2010250557083424 1106 58-59 0.267939175301926 1106 60-61 0.3099563113088948 1106 62-63 0.33931264012048956 1106 64-65 0.37160051504071845 1106 66-67 0.16182243216517378 1106 68-69 0.27307087246607864 1106 70-71 0.22392032390244054 1106 72-73 0.17695645018071815 1106 74-75 0.45489364400245336 1106 76-77 0.3644532915430645 1106 78-79 0.4076389081374714 1106 80-81 0.5393401130718374 1106 82-83 0.47499294698841155 1106 84-85 0.5078087500266406 1106 86-87 0.4524667902176951 1106 88-89 0.2962368734774259 1106 90-91 0.17898365278952255 1106 92-93 0.39489695825460913 1106 94-95 0.5831903705880315 1106 96-97 0.65917517176727 1106 98-99 0.6766223449842137 1106 100-101 0.0889346552463266 1107 1 -0.08446234555059817 1107 2 -0.05181177193660247 1107 3 -0.004004892816595884 1107 4 -0.13385776989115783 1107 5 -0.09027258596783838 1107 6 -0.059149126795368545 1107 7 -0.07471489830313516 1107 8 -0.11606593183483938 1107 9 -0.03185479918487033 1107 10-11 0.11090083318322996 1107 12-13 0.10874774968635847 1107 14-15 0.07635018520397807 1107 16-17 0.1246372340321713 1107 18-19 0.16890159329346233 1107 20-21 0.10376183918626225 1107 22-23 0.04130665878538764 1107 24-25 0.23593253881934828 1107 26-27 0.25211134942647107 1107 28-29 0.18985860453184955 1107 30-31 0.1326276962138877 1107 32-33 0.1924609633019685 1107 34-35 0.17886290720414166 1107 36-37 0.07643885860395017 1107 38-39 0.47496305035762276 1107 40-41 0.31350516684751284 1107 42-43 0.2439459868277183 1107 44-45 0.19158921323016642 1107 46-47 0.15016249514071234 1107 48-49 0.19097509926557166 1107 50-51 0.11309312546464412 1107 52-53 0.22019673289708663 1107 54-55 0.31206202378299963 1107 56-57 0.41291537264167744 1107 58-59 0.2838954265471969 1107 60-61 0.2232059693468571 1107 62-63 0.2473763402057827 1107 64-65 0.23207026231341388 1107 66-67 0.19999961215922468 1107 68-69 0.2791163808421899 1107 70-71 0.34015137607096335 1107 72-73 0.3342653711925152 1107 74-75 0.2915746980738696 1107 76-77 0.37247848591854194 1107 78-79 0.2923120685383829 1107 80-81 0.1573030267003972 1107 82-83 0.17546016452552138 1107 84-85 0.13366309546315236 1107 86-87 0.443642602363564 1107 88-89 0.3787768847157196 1107 90-91 0.2553413708337011 1107 92-93 0.3135106631531208 1107 94-95 0.3239935504432765 1107 96-97 0.45985808844046616 1107 98-99 0.5181761623104393 1107 100-101 0.31862009493899635 1108 1 0.07458898277972636 1108 2 0.008779540769623395 1108 3 0.059591738242566805 1108 4 0.08935711611842123 1108 5 0.018796157897142507 1108 6 0.06248352644290378 1108 7 0.16317826877799035 1108 8 0.13774065292893312 1108 9 0.28616500897965125 1108 10-11 0.3104045663538173 1108 12-13 0.23891878628587904 1108 14-15 0.259185213027628 1108 16-17 0.4171701771534302 1108 18-19 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-1.0640836672324383 2316 54-55 -1.0537001544570046 2316 56-57 -1.017228678272943 2316 58-59 -0.8762263935368964 2316 60-61 -0.7662716615269218 2316 62-63 -1.0057146096977547 2316 64-65 -1.0297727263848415 2316 66-67 -1.0761269711813313 2316 68-69 -1.1051361058925941 2316 70-71 -0.904435886158943 2316 72-73 -0.7673075131873652 2316 74-75 -0.5930488170633801 2316 76-77 -0.6857875928415424 2316 78-79 -0.5388727324819733 2316 80-81 -0.3977611840960371 2316 82-83 -0.42182982077681075 2316 84-85 -0.3707587349545278 2316 86-87 -0.46071361250746534 2316 88-89 -0.5553055518748344 2316 90-91 -0.39023143218508594 2316 92-93 -0.5602708499708093 2316 94-95 -0.6339710019063247 2316 96-97 -0.6871226429631179 2316 98-99 -0.7579308071938584 2316 100-101 -0.09127948040328704 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7938.0 3 1960.0 4 1974.0 5 2257.0 6 2597.0 7 3093.0 8 3993.0 9 4660.0 10 5520.0 11 6722.0 12 8489.0 13 11054.0 14 11302.0 15 11559.0 16 14488.0 17 18185.0 18 16203.0 19 12119.0 20 12460.0 21 13476.0 22 14506.0 23 16043.0 24 18163.0 25 20716.0 26 23611.0 27 27984.0 28 33055.0 29 39863.0 30 47708.0 31 58838.0 32 73653.0 33 95614.0 34 132520.0 35 195062.0 36 304843.0 37 455707.0 38 373645.0 39 43188.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.20366195747371 18.067851469875144 14.116309724710241 23.6121768479409 2 15.571665579932853 19.88251130794798 39.57725743260538 24.96856567951379 3 19.437316261815994 23.877299558981647 29.112395689024613 27.572988490177742 4 13.179117028731605 15.839149731371174 35.68124270030367 35.300490539593554 5 14.790358558960984 36.771474148411954 32.732183958530555 15.70598333409651 6 33.31110072863511 34.46036614252795 17.103345205762057 15.125187923074876 7 30.060117901671425 32.068638416823674 19.70170727744986 18.16953640405504 8 27.22830499556223 33.98104714588992 19.418528266103788 19.372119592444072 9 27.413200783272995 16.77822486135377 19.66507328943842 36.143501065934814 10-11 26.127457044753754 25.71388222991473 26.286299838239447 21.872360887092068 12-13 26.778988760399784 23.053935470499695 27.069215370550648 23.097860398549873 14-15 23.439956151961095 24.41967409963757 25.493162049597025 26.64720769880431 16-17 23.44485056796356 27.887811269095288 25.68204869219255 22.985289470748604 18-19 23.19358722150516 26.14323562100681 28.119565879659557 22.543611277828475 20-21 24.027691317649246 25.33849432196294 27.794781819260166 22.83903254112765 22-23 23.651847119360593 25.207708107411364 27.913330960860737 23.227113812367307 24-25 23.700563876495586 25.447455221778498 27.842937669948903 23.009043231777014 26-27 23.293565549356103 25.76243900149383 27.887429981759702 23.056565467390364 28-29 23.000315247469263 25.9688488797039 28.165376487209127 22.865459385617708 30-31 23.074379810227043 25.405330435541966 27.97549361376034 23.544796140470655 32-33 22.994207483522175 25.685348151116354 27.867267634997205 23.453176730364266 34-35 23.808159154631387 25.78215195159296 27.32969399368594 23.079994900089716 36-37 23.80729309885263 25.33286831938682 27.48548627139232 23.374352310368234 38-39 23.311820726140052 25.643077725659353 27.723657397662137 23.321444150538458 40-41 23.31020897146575 25.501205383993874 27.883527386460134 23.30505825808024 42-43 23.460377112823323 25.573305818838033 27.185653057888633 23.78066401045001 44-45 23.36835621940181 25.732676200479382 27.16827495600184 23.730692624116966 46-47 23.49804176154574 26.110064640721852 26.830009726202597 23.561883871529815 48-49 23.956924581713487 25.94315603772109 26.64071792051514 23.459201460050284 50-51 23.336824606166157 26.131708736035886 26.81386534960117 23.71760130819679 52-53 23.817981493565657 26.131449742518566 26.445613165585886 23.604955598329898 54-55 23.21997338299842 25.851417368214108 26.65088755733627 24.277721691451205 56-57 23.546682297816478 26.367967504187916 26.617018726479536 23.46833147151607 58-59 23.832335385405454 25.642919789423274 26.697699371317384 23.82704545385389 60-61 23.601802506205154 25.75278918908043 27.243920659218844 23.40148764549557 62-63 23.397384850608397 26.00845890372458 26.977476025767384 23.616680219899642 64-65 23.11923429681725 25.886584455284794 26.935102786087086 24.059078461810866 66-67 23.310120223934558 26.151932303386776 26.6382200477223 23.899727424956367 68-69 23.127216062848106 25.909288536067947 27.04158889363648 23.92190650744747 70-71 23.2052130723143 25.993215454073137 26.923150798971403 23.878420674641156 72-73 23.45711890596311 25.83241809912824 26.812790277975896 23.897672716932757 74-75 23.154060895037972 26.439327252855467 26.442606569595767 23.964005282510794 76-77 23.345420993654855 26.083656601162243 26.56799045476846 24.002931950414442 78-79 23.345815155392128 26.17628216535573 26.63620202943562 23.84170064981652 80-81 23.25498791464883 26.608751887494662 26.49193736268908 23.644322835167422 82-83 23.408644329475635 26.318040004805244 26.395996381550212 23.87731928416891 84-85 23.234382340006306 26.14551519321297 26.349132676168345 24.27096979061238 86-87 23.479098627276766 25.95193478147852 26.600148283522216 23.9688183077225 88-89 23.192058262763716 26.039831874164648 26.454080742462878 24.31402912060876 90-91 23.218387358363195 26.169295656276105 26.643202440554354 23.96911454480635 92-93 23.458824890799928 25.82347758807158 26.611999674865235 24.105697846263258 94-95 23.25173158444222 25.734545745007047 26.701899970180836 24.31182270036989 96-97 23.44178583558788 25.67909302788611 26.718363140203554 24.160757996322452 98-99 23.2620451124579 25.864778806167465 26.277662404707907 24.595513676666727 100-101 23.39542273345118 25.23271815365534 26.934204720780368 24.43765439211311 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2600.0 1 1527.5 2 522.0 3 588.5 4 535.0 5 533.0 6 752.0 7 1420.0 8 2234.5 9 2567.0 10 2369.0 11 2142.5 12 2255.5 13 2473.5 14 2673.0 15 2846.5 16 2952.0 17 2997.0 18 3074.5 19 3183.0 20 3308.5 21 3593.5 22 4073.0 23 4813.5 24 5561.5 25 6350.0 26 7622.5 27 9206.5 28 10822.5 29 13086.5 30 16169.5 31 19285.0 32 22785.5 33 26003.0 34 28973.5 35 32107.0 36 35597.0 37 39535.5 38 43208.0 39 45321.0 40 47323.5 41 54246.0 42 61687.5 43 66663.5 44 71065.5 45 75222.0 46 77865.5 47 82495.0 48 94857.0 49 97488.0 50 90697.0 51 87496.5 52 84322.5 53 84704.0 54 86353.5 55 81122.0 56 71917.0 57 65353.5 58 59444.5 59 52074.5 60 44892.0 61 38076.5 62 32431.0 63 26569.5 64 20903.5 65 16562.0 66 12788.5 67 10787.5 68 8251.5 69 5364.5 70 4100.5 71 3354.0 72 2883.0 73 2437.0 74 1895.0 75 1602.0 76 1148.0 77 655.0 78 379.5 79 244.5 80 167.5 81 128.0 82 102.0 83 76.0 84 57.5 85 39.0 86 29.5 87 14.0 88 7.0 89 8.0 90 7.5 91 9.0 92 11.0 93 5.5 94 1.0 95 2.5 96 3.0 97 4.0 98 3.5 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28846915986329535 2 0.17708192486840083 3 0.17792117569716098 4 0.19908894660033474 5 0.2141022114259338 6 0.10588547956191106 7 0.12336987182774842 8 0.13656475985770036 9 0.1393156375741921 10-11 0.12658700000466253 12-13 0.1155601766156744 14-15 0.1318090051613926 16-17 0.10681798048275573 18-19 0.13621507201238361 20-21 0.1608097837996615 22-23 0.16523916317367363 24-25 0.18897131160917022 26-27 0.16931885470236901 28-29 0.16761704052182752 30-31 0.15707978011628287 32-33 0.16593853886430712 34-35 0.34863878178079705 36-37 0.46937433850715926 38-39 0.43620061824811057 40-41 0.4263161084871571 42-43 0.4500715694456748 44-45 0.4540113858362435 46-47 0.4576481394275377 48-49 0.468535087678399 50-51 0.4626603318770777 52-53 0.46872158786256796 54-55 0.4507942576593294 56-57 0.41328440811835304 58-59 0.402677210143745 60-61 0.3559589140094276 62-63 0.4652247094094005 64-65 0.46382595802813353 66-67 0.47224177883875657 68-69 0.4827557267212801 70-71 0.4761815952293253 72-73 0.4695375261683071 74-75 0.4745963436638894 76-77 0.45098075784349834 78-79 0.43818218270490544 80-81 0.4351748672351814 82-83 0.4476703795744998 84-85 0.4494188188010836 86-87 0.450910820274435 88-89 0.4579278897037911 90-91 0.474316593387636 92-93 0.47886253537675366 94-95 0.47727728381131773 96-97 0.4753423444005651 98-99 0.47319759228262237 100-101 0.4561794504772073 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2144770.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.629477314744875 #Duplication Level Percentage of deduplicated Percentage of total 1 69.1146433774091 27.38977191841716 2 14.370971253337949 11.390281585500139 3 6.036473832972831 7.176669084745437 4 3.068885935799073 4.8647338229715595 5 1.7888888979373456 3.54463659997035 6 1.1845297629270741 2.816537722113517 7 0.7818443064806658 2.168885684513659 8 0.5498702958255377 1.7432857931576162 9 0.4294063295034763 1.531543355447929 >10 2.3348221601744807 16.955258332104336 >50 0.19233843765583672 5.291764631210706 >100 0.13514863049613698 10.278783454701399 >500 0.009457692800499296 2.5285175876051094 >1k 0.002482644360131065 1.7656052215394877 >5k 2.364423200124824E-4 0.5537252060015709 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6771 0.3156981867519594 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5048 0.23536323242119203 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3842 0.17913342689425904 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3538 0.16495941289742022 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 2282 0.10639835506837562 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 2226 0.10378735249001059 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 9.325009208446594E-5 0.0 0.0 0.0 0.0 6 9.325009208446594E-5 0.0 0.0 0.0 0.0 7 2.3312523021116484E-4 0.0 0.0 0.0 4.662504604223297E-5 8 2.3312523021116484E-4 0.0 0.0 0.0 4.662504604223297E-5 9 2.3312523021116484E-4 0.0 0.0 0.0 4.662504604223297E-5 10-11 2.5643775323228133E-4 0.0 0.0 0.0 4.662504604223297E-5 12-13 3.263753222956308E-4 0.0 0.0 0.0 4.662504604223297E-5 14-15 3.263753222956308E-4 0.0 0.0 6.993756906334945E-5 4.662504604223297E-5 16-17 3.263753222956308E-4 0.0 0.0 1.398751381266989E-4 4.662504604223297E-5 18-19 3.9631289135898023E-4 0.0 0.0 1.398751381266989E-4 4.662504604223297E-5 20-21 4.662504604223297E-4 0.0 0.0 2.0981270719004836E-4 4.662504604223297E-5 22-23 4.662504604223297E-4 0.0 0.0 4.662504604223297E-4 4.662504604223297E-5 24-25 6.527506445912616E-4 0.0 0.0 8.15938305739077E-4 4.662504604223297E-5 26-27 6.993756906334945E-4 0.0 0.0 0.001352126335224756 4.662504604223297E-5 28-29 8.625633517813099E-4 0.0 0.0 0.0031238780848296087 4.662504604223297E-5 30-31 9.791259668868923E-4 0.0 0.0 0.00920844659334101 4.662504604223297E-5 32-33 9.791259668868923E-4 0.0 0.0 0.020794770534835902 4.662504604223297E-5 34-35 0.0010257510129291253 0.0 0.0 0.03702028655753298 4.662504604223297E-5 36-37 0.0010723760589713583 0.0 0.0 0.06396956316994364 4.662504604223297E-5 38-39 0.0012122511970980572 0.0 0.0 0.10642166759139673 4.662504604223297E-5 40-41 0.0012122511970980572 0.0 0.0 0.15241727551205958 4.662504604223297E-5 42-43 0.0012355637201191738 0.0 0.0 0.20228276225422773 4.662504604223297E-5 44-45 0.001398751381266989 0.0 0.0 0.2652498869342633 4.662504604223297E-5 46-47 0.001538626519393688 0.0 0.0 0.34432596502189045 4.662504604223297E-5 48-49 0.001585251565435921 0.0 0.0 0.42729523445404405 4.662504604223297E-5 50-51 0.001585251565435921 0.0 0.0 0.5222937657650937 4.662504604223297E-5 52-53 0.001585251565435921 0.0 0.0 0.6134457307776591 4.662504604223297E-5 54-55 0.001585251565435921 0.0 0.0 0.7201238361222881 4.662504604223297E-5 56-57 0.0017251267035626198 0.0 0.0 0.849181963567189 4.662504604223297E-5 58-59 0.0017251267035626198 0.0 0.0 0.9915049166111052 4.662504604223297E-5 60-61 0.0017251267035626198 0.0 0.0 1.1531539512395268 4.662504604223297E-5 62-63 0.0017717517496048528 0.0 0.0 1.3141502352233574 4.662504604223297E-5 64-65 0.0017717517496048528 0.0 0.0 1.4872690311781684 4.662504604223297E-5 66-67 0.0018650018416893187 0.0 0.0 1.6907873571525154 4.662504604223297E-5 68-69 0.0018650018416893187 0.0 0.0 1.921115084601146 4.662504604223297E-5 70-71 0.0018883143647104353 0.0 0.0 2.1759675862679915 4.662504604223297E-5 72-73 0.0019116268877315517 0.0 0.0 2.447092229003576 4.662504604223297E-5 74-75 0.0019116268877315517 0.0 0.0 2.7339761373014357 4.662504604223297E-5 76-77 0.0019116268877315517 0.0 0.0 3.0397431892463995 4.662504604223297E-5 78-79 0.001934939410752668 0.0 0.0 3.3904334730530543 4.662504604223297E-5 80-81 0.002214689687006066 0.0 0.0 3.7792630445222564 4.662504604223297E-5 82-83 0.0022380022100271825 0.0 0.0 4.1777440005222 4.662504604223297E-5 84-85 0.002307939779090532 0.0 0.0 4.5823561500766985 4.662504604223297E-5 86-87 0.0024011898711749978 0.0 0.0 5.014383826704028 4.662504604223297E-5 88-89 0.0024245023941961144 0.0 0.0 5.4826857891522165 4.662504604223297E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10385 0.0 28.196749 1 TACATGG 10520 0.0 27.454105 2 GTATCAA 11800 0.0 26.94717 1 ACATGGG 10820 0.0 25.903948 3 CATGGGG 5595 0.0 24.072092 4 GGTATCA 8655 0.0 23.523968 1 TATCAAC 14335 0.0 21.934189 2 AGGTACG 130 2.9748087E-4 21.887869 5 GAGTACT 6105 0.0 21.811556 12-13 TCAACGC 14425 0.0 21.698198 4 ATCAACG 14510 0.0 21.604279 3 CAACGCA 14500 0.0 21.553263 5 CATGGGA 5335 0.0 21.245115 4 AACGCAG 14730 0.0 21.233044 6 AGTACTT 6440 0.0 20.419405 12-13 ATGGGGA 3335 0.0 19.765806 5 GTATATA 800 0.0 19.576918 1 GTACTTT 6820 0.0 19.319342 14-15 AGAGTAC 12405 0.0 19.156887 10-11 ACGCAGA 16305 0.0 19.152948 7 >>END_MODULE