FastQCFastQC Report
Fri 27 May 2016
SRR939251_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939251_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2144770
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70900.33057157643943175No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56690.2643173860134187No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT40750.18999706262209934No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39360.18351618122222896No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG23390.1090559826927829No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC22000.10257510129291253No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG21800.10164260037206786No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG21660.1009898497274766No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA127450.026.0231691
GGTATCA94350.025.5197161
GTACATG104550.025.3056771
TACATGG107150.024.4925652
ACATGGG106800.023.5508163
GAGTACT67150.021.76737412-13
TCAACGC152400.021.642194
ATCAACG152400.021.548773
TATCAAC153850.021.407372
CAACGCA153200.021.4052665
AACGCAG155850.021.04136
GTACTTT71000.020.38650914-15
CATGGGG55950.020.3569414
AGTACTT68300.019.59430112-13
GAGTGCA13900.019.460839
CCAAGAC13700.019.398533
ACGCAGA172150.019.021447
GTACCGT1250.006064361418.9828456
TACCGTA1250.006064361418.9828457
AGAGTAC129300.018.902110-11
Produced by FastQC (version 0.11.5)