##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939251_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2144770 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.33660252614499 34.0 31.0 34.0 31.0 34.0 2 32.54221105293342 34.0 31.0 34.0 31.0 34.0 3 32.62784680874872 34.0 31.0 34.0 31.0 34.0 4 36.148004681154625 37.0 35.0 37.0 35.0 37.0 5 36.091061512423245 37.0 35.0 37.0 35.0 37.0 6 36.12187693785348 37.0 36.0 37.0 35.0 37.0 7 36.060758496249015 37.0 35.0 37.0 35.0 37.0 8 36.03311077644689 37.0 35.0 37.0 35.0 37.0 9 37.73839805666808 39.0 38.0 39.0 35.0 39.0 10-11 37.66160054458054 39.0 37.0 39.0 35.0 39.0 12-13 37.58420180252428 39.0 37.0 39.0 35.0 39.0 14-15 38.890956372944416 40.0 38.0 41.0 35.0 41.0 16-17 38.8947705348359 40.0 38.0 41.0 35.0 41.0 18-19 38.986020645570385 40.0 38.5 41.0 35.0 41.0 20-21 38.95924038474988 40.0 38.0 41.0 35.0 41.0 22-23 38.88107093068254 40.0 38.0 41.0 35.0 41.0 24-25 38.80492966611804 40.0 38.0 41.0 34.5 41.0 26-27 38.64818302195573 40.0 38.0 41.0 34.0 41.0 28-29 38.482617716585 40.0 38.0 41.0 34.0 41.0 30-31 38.288618593135865 40.0 38.0 41.0 34.0 41.0 32-33 38.25071266382876 40.0 38.0 41.0 34.0 41.0 34-35 38.15203285200744 40.0 38.0 41.0 33.5 41.0 36-37 37.999839609841615 40.0 37.5 41.0 33.0 41.0 38-39 37.86988068650717 40.0 37.0 41.0 33.0 41.0 40-41 37.67935256461066 40.0 37.0 41.0 33.0 41.0 42-43 37.48327839348741 40.0 37.0 41.0 32.5 41.0 44-45 37.2263303291262 40.0 36.5 41.0 31.5 41.0 46-47 36.90138523011791 40.0 36.0 41.0 31.0 41.0 48-49 36.57505047161234 39.0 36.0 41.0 30.5 41.0 50-51 36.30683569800025 39.0 35.0 41.0 30.0 41.0 52-53 36.031307552791205 39.0 35.0 40.5 29.5 41.0 54-55 35.75669372473505 38.5 35.0 40.0 29.0 41.0 56-57 35.42860749637491 38.0 35.0 40.0 28.0 41.0 58-59 35.08816586393879 38.0 34.0 40.0 28.0 41.0 60-61 34.730014174014 37.5 34.0 40.0 27.0 41.0 62-63 34.36926337089758 37.0 34.0 40.0 26.0 41.0 64-65 34.04295891867193 36.0 33.5 39.0 26.0 41.0 66-67 33.619293444052275 36.0 33.0 39.0 26.0 40.0 68-69 33.14973563598895 35.0 33.0 38.5 25.0 40.0 70-71 32.65965814516242 35.0 32.0 37.5 24.0 40.0 72-73 32.17446695916112 35.0 31.5 37.0 22.5 39.0 74-75 31.659965870466294 35.0 31.0 36.5 21.5 39.0 76-77 29.73218293803065 32.5 28.5 35.0 18.0 36.5 78-79 30.70217505839787 34.0 30.5 35.0 19.5 37.0 80-81 30.72263296297505 34.0 31.0 35.0 19.5 37.0 82-83 30.389723373601832 34.0 30.5 35.0 18.0 36.5 84-85 29.973904661105855 34.0 30.0 35.0 13.0 36.0 86-87 29.388810688325556 34.0 29.5 35.0 7.0 35.5 88-89 28.830770432260802 34.0 29.0 35.0 2.0 35.0 90-91 28.361929018029905 33.0 28.5 35.0 2.0 35.0 92-93 28.003622766077484 33.0 27.5 35.0 2.0 35.0 94-95 27.481294264653087 33.0 27.0 35.0 2.0 35.0 96-97 26.7510154935028 33.0 25.5 34.5 2.0 35.0 98-99 25.747704182732882 32.0 24.0 34.0 2.0 35.0 100-101 21.397050965837828 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.29494918373130474 1101 2 0.2873291593162719 1101 3 0.25776752221656807 1101 4 0.24403691757527213 1101 5 0.19878172028744956 1101 6 0.25067487365566876 1101 7 0.21358954740787084 1101 8 0.1705956363877732 1101 9 0.28918592401009846 1101 10-11 0.29930335381910567 1101 12-13 0.2499101166337354 1101 14-15 0.30551239496312377 1101 16-17 0.35096508548004834 1101 18-19 0.4295723291461897 1101 20-21 0.36251535268760193 1101 22-23 0.2911731274058411 1101 24-25 0.32274022398282654 1101 26-27 0.3209471087147122 1101 28-29 0.4039752764246387 1101 30-31 0.3673468112235838 1101 32-33 0.5368655861500429 1101 34-35 0.5476010925243315 1101 36-37 0.5811462489481372 1101 38-39 0.6083431531268246 1101 40-41 0.6181031680568267 1101 42-43 0.5963199342691112 1101 44-45 0.7624105416258189 1101 46-47 0.6264885140240395 1101 48-49 0.6566068531972533 1101 50-51 0.6148303500526566 1101 52-53 0.6228597908492688 1101 54-55 0.6214245935016933 1101 56-57 0.6961832219848603 1101 58-59 0.6399842126672297 1101 60-61 0.5985197450111883 1101 62-63 0.4803269312871876 1101 64-65 0.43081819263785803 1101 66-67 0.4841393237182743 1101 68-69 0.6100039442863334 1101 70-71 0.5183854227345108 1101 72-73 0.7073562811229692 1101 74-75 0.7327250175161986 1101 76-77 0.7890654751316362 1101 78-79 0.8775815198020318 1101 80-81 0.9544216856047107 1101 82-83 1.0325294020351627 1101 84-85 1.0750050452380187 1101 86-87 0.9008015043065747 1101 88-89 1.074517758160102 1101 90-91 1.1602047944182026 1101 92-93 1.1644686866795055 1101 94-95 1.1104978704988469 1101 96-97 1.128816074964309 1101 98-99 0.9951540580787288 1101 100-101 0.7867816391276001 1102 1 0.18614168656726093 1102 2 0.21440614374478173 1102 3 0.21097599263235622 1102 4 0.17429906503422643 1102 5 0.23657228793680218 1102 6 0.19874736636032253 1102 7 0.2153347720883687 1102 8 0.23358620295932297 1102 9 0.36308650251826435 1102 10-11 0.3093985503421308 1102 12-13 0.32186109298415744 1102 14-15 0.3783130804427586 1102 16-17 0.34814610437654636 1102 18-19 0.34363490071871894 1102 20-21 0.3613126646589748 1102 22-23 0.35246845218684086 1102 24-25 0.36591830049032836 1102 26-27 0.3232682817902415 1102 28-29 0.34691960545486467 1102 30-31 0.29545861661635087 1102 32-33 0.4363227800486271 1102 34-35 0.38745443113887745 1102 36-37 0.3996839732311983 1102 38-39 0.5074690588140953 1102 40-41 0.4736147584288517 1102 42-43 0.527920437600848 1102 44-45 0.5811100703585694 1102 46-47 0.5831813711861429 1102 48-49 0.4571779515005332 1102 50-51 0.44927152517599467 1102 52-53 0.40169385573810956 1102 54-55 0.3530066395398421 1102 56-57 0.20105985451227326 1102 58-59 0.14478041450648504 1102 60-61 0.28982770697569293 1102 62-63 0.2949556163464777 1102 64-65 0.3890101323242732 1102 66-67 0.23513507449900573 1102 68-69 0.2215284359024139 1102 70-71 0.1676929374127667 1102 72-73 0.0821550696778317 1102 74-75 0.11765160598860547 1102 76-77 0.22857472668164647 1102 78-79 0.20198812454919235 1102 80-81 0.31223491396050207 1102 82-83 0.2828438496501313 1102 84-85 0.4008766471424856 1102 86-87 0.3043269821079768 1102 88-89 0.43954051317388476 1102 90-91 0.5312995948503989 1102 92-93 0.39733183056286947 1102 94-95 0.40457146255762666 1102 96-97 0.4014336694804719 1102 98-99 -0.06901656275995904 1102 100-101 -0.23336671978996648 1103 1 0.11894137363047719 1103 2 0.22986296307944087 1103 3 0.23836591218788072 1103 4 0.16918681009251202 1103 5 0.1965178102216214 1103 6 0.1592446623511421 1103 7 0.21913278792920465 1103 8 0.21278815054559175 1103 9 0.275787792587451 1103 10-11 0.20340472957158795 1103 12-13 0.22643167151967702 1103 14-15 0.36337861748394573 1103 16-17 0.23268228848768757 1103 18-19 0.22208487774140195 1103 20-21 0.2503453274362002 1103 22-23 0.3135886800200893 1103 24-25 0.2743989987464346 1103 26-27 0.28355289843472065 1103 28-29 0.4216308783347884 1103 30-31 0.3078635867140349 1103 32-33 0.5042342768182877 1103 34-35 0.4650291233302468 1103 36-37 0.35591535881925296 1103 38-39 0.37799305301967934 1103 40-41 0.3643997400072436 1103 42-43 0.4673732116550795 1103 44-45 0.5361643652092667 1103 46-47 0.6313737160017752 1103 48-49 0.4764125125701142 1103 50-51 0.4569442804349393 1103 52-53 0.25576600929339577 1103 54-55 0.33699128846387794 1103 56-57 0.4591838080106285 1103 58-59 0.5347262071127119 1103 60-61 0.5849236790276322 1103 62-63 0.4627751243297027 1103 64-65 0.46855141278909684 1103 66-67 0.2896986564803328 1103 68-69 0.26878340146823376 1103 70-71 0.4151709976499234 1103 72-73 0.3169009914022922 1103 74-75 0.1863796324355853 1103 76-77 0.322854487081468 1103 78-79 0.36566575195405804 1103 80-81 0.5025256843924275 1103 82-83 0.407074427454198 1103 84-85 0.35192501718387703 1103 86-87 0.41287215997151705 1103 88-89 0.6037210179116634 1103 90-91 0.6479075063630724 1103 92-93 0.3757355349202882 1103 94-95 0.3281631277534274 1103 96-97 0.23083819911326486 1103 98-99 0.007243890330528302 1103 100-101 -0.19299819219889613 1104 1 0.19022449092695837 1104 2 0.18504784742403046 1104 3 0.2146876329593539 1104 4 0.09864078096448736 1104 5 0.1583080689801264 1104 6 0.0311535682777091 1104 7 0.07626350236476043 1104 8 0.12450446796732706 1104 9 0.2919636871949507 1104 10-11 0.10754864803873687 1104 12-13 0.07859291840519944 1104 14-15 0.17795146804390782 1104 16-17 0.1890301600002786 1104 18-19 0.14642973992942387 1104 20-21 0.2139467872479699 1104 22-23 0.1692611069804144 1104 24-25 0.2198527949701088 1104 26-27 0.2525778855427845 1104 28-29 0.24814039770059537 1104 30-31 0.2420338367711281 1104 32-33 0.18311934893563375 1104 34-35 0.23903034690177094 1104 36-37 0.23436569617982883 1104 38-39 0.2489463020464484 1104 40-41 0.400868165351298 1104 42-43 0.38222557700279225 1104 44-45 0.31867633481839164 1104 46-47 0.24040385988510593 1104 48-49 0.18828596179138657 1104 50-51 0.24015251804757298 1104 52-53 0.3059205575381725 1104 54-55 0.24910017702896425 1104 56-57 0.24459142577681092 1104 58-59 0.13791829234108377 1104 60-61 0.3099536224271233 1104 62-63 0.44221299965561656 1104 64-65 0.4018563011847007 1104 66-67 0.22601170684208682 1104 68-69 0.27162182388770617 1104 70-71 0.2856221328122075 1104 72-73 0.23023887695907774 1104 74-75 0.18886630387665448 1104 76-77 0.25616441340881835 1104 78-79 0.441816932276879 1104 80-81 0.43834191717690985 1104 82-83 0.4060747209460196 1104 84-85 0.35189156014994083 1104 86-87 0.42694452430647445 1104 88-89 0.7828488149972124 1104 90-91 0.7784089278348922 1104 92-93 0.6748916684342632 1104 94-95 0.5533892921357157 1104 96-97 0.17437861469354843 1104 98-99 0.5082221533343336 1104 100-101 0.3170399514033413 1105 1 0.08105272806633224 1105 2 0.10706122691356512 1105 3 0.10018467030151612 1105 4 0.08086978490941732 1105 5 0.14051528867626217 1105 6 0.09027700971373065 1105 7 0.13403037921642635 1105 8 0.029823191331132648 1105 9 0.16862919203501292 1105 10-11 0.15357084420787004 1105 12-13 0.18421335361488644 1105 14-15 0.1386873399683708 1105 16-17 0.13131229770841202 1105 18-19 0.12169471561028189 1105 20-21 0.16500797722246574 1105 22-23 0.18983732016502586 1105 24-25 0.19582427344727193 1105 26-27 0.2567421427395047 1105 28-29 0.17481263585392526 1105 30-31 0.269448818789229 1105 32-33 0.21459412277552303 1105 34-35 0.30092583399757444 1105 36-37 0.12220998633018354 1105 38-39 0.19054322611052044 1105 40-41 0.2417515562478556 1105 42-43 0.2213132526630588 1105 44-45 0.113624666541007 1105 46-47 0.06410145886481189 1105 48-49 0.22403440916856 1105 50-51 0.29683562852144263 1105 52-53 0.12977114135604495 1105 54-55 0.3068234853830276 1105 56-57 0.34822355318693354 1105 58-59 0.23421605925754818 1105 60-61 0.15022111319851916 1105 62-63 0.3147221688439785 1105 64-65 0.21865175897078615 1105 66-67 0.2151557244912894 1105 68-69 0.2453970540085848 1105 70-71 0.23683333704548204 1105 72-73 0.3247310267079655 1105 74-75 0.21924641921435395 1105 76-77 0.3890839780407056 1105 78-79 0.46311301586502296 1105 80-81 0.4041332201126373 1105 82-83 0.3864599852879742 1105 84-85 0.3742728954055714 1105 86-87 0.4537540032272389 1105 88-89 0.3182581212282969 1105 90-91 0.5440044695893569 1105 92-93 0.6327861811800304 1105 94-95 0.6385171854800333 1105 96-97 0.556451069105055 1105 98-99 0.6458530568657572 1105 100-101 0.39623559772219963 1106 1 0.030938442885151574 1106 2 0.08598284127338474 1106 3 0.0893564992375886 1106 4 0.025272609852507344 1106 5 0.026113956437576746 1106 6 0.04725586928756087 1106 7 0.09126393147973744 1106 8 0.1124205902937021 1106 9 0.09505379180364315 1106 10-11 0.08261420727090041 1106 12-13 0.1208454859431356 1106 14-15 0.13293063212793754 1106 16-17 0.1310326488671265 1106 18-19 0.10792358451352158 1106 20-21 0.21327760628542336 1106 22-23 0.2553008048834684 1106 24-25 0.18356707114850224 1106 26-27 0.1084949042426544 1106 28-29 0.12841565111683195 1106 30-31 0.19915666854852532 1106 32-33 0.06857521207656703 1106 34-35 0.2640749271796423 1106 36-37 0.17122643406686677 1106 38-39 0.09651894167569708 1106 40-41 0.05353249254669379 1106 42-43 0.10831552903727726 1106 44-45 0.2256546978878049 1106 46-47 0.19775984413181646 1106 48-49 0.20693291459826924 1106 50-51 0.12844387757916564 1106 52-53 0.33047737712870884 1106 54-55 0.19704114034440323 1106 56-57 0.32718318486212894 1106 58-59 0.18957276896385622 1106 60-61 0.15875448447142304 1106 62-63 0.2236058591824488 1106 64-65 0.20062688678360274 1106 66-67 0.2631482702986574 1106 68-69 0.23726997821652418 1106 70-71 0.22745822042794828 1106 72-73 0.2312942613984177 1106 74-75 0.1262569987568405 1106 76-77 0.257815552470678 1106 78-79 0.35720237288260037 1106 80-81 0.33444841397762204 1106 82-83 0.471223671521507 1106 84-85 0.3363101570393354 1106 86-87 0.32836823243200186 1106 88-89 0.5470141386929406 1106 90-91 0.6314377643611593 1106 92-93 0.6351015056566531 1106 94-95 0.8027058073406899 1106 96-97 0.8220659233797853 1106 98-99 0.8231322299075181 1106 100-101 0.7453996832541634 1107 1 -0.0024471268762198406 1107 2 0.04797054183327987 1107 3 0.03120078841546814 1107 4 0.014288184516644264 1107 5 -0.05600663359526692 1107 6 -0.018027203647179135 1107 7 -0.01320362634039185 1107 8 -0.0463377796610942 1107 9 0.010085122466954033 1107 10-11 0.03735611827345764 1107 12-13 0.06059546747382427 1107 14-15 0.07233215937184667 1107 16-17 0.013283307605796324 1107 18-19 0.138955156956861 1107 20-21 0.1191167061472882 1107 22-23 0.04201308887651578 1107 24-25 -0.03491969922986016 1107 26-27 -0.08699925609780479 1107 28-29 0.03425754935877734 1107 30-31 0.15310798121264924 1107 32-33 0.07598789702150555 1107 34-35 -0.00706146138774244 1107 36-37 -0.0048681243151804665 1107 38-39 0.11072989923657417 1107 40-41 0.09753992952158086 1107 42-43 0.07531479250364015 1107 44-45 0.020481164742669478 1107 46-47 -0.041226403509149634 1107 48-49 0.05026012134482727 1107 50-51 0.08935733599636109 1107 52-53 0.18866957882698898 1107 54-55 0.045256781274176205 1107 56-57 -0.02543710630371976 1107 58-59 -0.10545954672923585 1107 60-61 -0.0072497869759260425 1107 62-63 0.13462811861690938 1107 64-65 0.07502652232258811 1107 66-67 0.11400547008284434 1107 68-69 0.06044985584895102 1107 70-71 0.0487259089883878 1107 72-73 0.23084584897540594 1107 74-75 0.159960464494592 1107 76-77 0.17011790288606932 1107 78-79 0.277817703080256 1107 80-81 0.29710906897765454 1107 82-83 0.41828033535994535 1107 84-85 0.30460417499822157 1107 86-87 0.43419658651439264 1107 88-89 0.6539707996210602 1107 90-91 0.6832112657166931 1107 92-93 0.6330117873527854 1107 94-95 0.8786019046079048 1107 96-97 0.7576642282330717 1107 98-99 0.8909508590594761 1107 100-101 0.5508119741871873 1108 1 -0.0067142581884880315 1108 2 0.1137869486983405 1108 3 0.07204943462333091 1108 4 0.12023037361682043 1108 5 0.04941504139311803 1108 6 0.06254577828708108 1108 7 0.10492444875938389 1108 8 0.1181125812937509 1108 9 0.11153353973535474 1108 10-11 0.09244147608136899 1108 12-13 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-0.6987737281795141 2316 80-81 -0.45328543246496267 2316 82-83 -0.43459782258119617 2316 84-85 -0.4723451906122236 2316 86-87 -0.6237674754893057 2316 88-89 -0.7695794609251472 2316 90-91 -0.9785852672471727 2316 92-93 -0.7490997038672091 2316 94-95 -0.5136027214606642 2316 96-97 -0.2731803453721149 2316 98-99 -0.11420667182732203 2316 100-101 0.4202988744146907 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 7.0 4 22.0 5 50.0 6 120.0 7 294.0 8 617.0 9 1187.0 10 1887.0 11 2952.0 12 4218.0 13 5966.0 14 7453.0 15 9604.0 16 12723.0 17 17800.0 18 17115.0 19 11977.0 20 11531.0 21 12479.0 22 13284.0 23 14557.0 24 16940.0 25 20266.0 26 23729.0 27 28824.0 28 35384.0 29 42943.0 30 51787.0 31 63989.0 32 79663.0 33 103400.0 34 143185.0 35 210116.0 36 330098.0 37 499330.0 38 332294.0 39 16978.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.348575270840925 18.66374390552399 14.567311491491933 23.420369332143157 2 16.0185474433156 20.33765858343785 38.742242757964725 24.901551215281824 3 19.524284655231096 23.96084428633373 29.326128209551605 27.188742848883564 4 13.568074898473961 16.31755386358444 35.319917753418785 34.79445348452281 5 14.786480601649593 36.86190127612751 32.72966332054253 15.621954801680365 6 33.314434648004216 34.77034833571898 16.780540570783813 15.134676445492989 7 29.95598595653613 32.22247606969512 19.552632683224775 18.268905290543973 8 27.182355217575775 34.2377970598246 19.167882803284268 19.41196491931536 9 27.230052639676984 16.651062817924533 19.64896002834803 36.469924514050454 10-11 26.123453796910624 25.873053987140814 26.13399105731617 21.869501158632392 12-13 26.73533758864587 23.087533861439688 27.048308210204357 23.128820339710085 14-15 23.33448343388942 24.562231319414007 25.41858220576853 26.68470304092804 16-17 23.3882067593766 27.986146662973937 25.656508858358578 22.969137719290885 18-19 23.110251034027442 26.158364840222887 28.174222958276623 22.557161167473048 20-21 23.934361260181745 25.33425495507677 27.885064599001293 22.846319185740196 22-23 23.58488322757219 25.205196827631866 28.048159010057024 23.161760934738922 24-25 23.676035192584752 25.472428279022925 27.959851172853035 22.891685355539288 26-27 23.269014814419364 25.798541893915356 27.978823821778438 22.953619469886842 28-29 22.938799964564964 25.982925908139332 28.261794971022535 22.816479156273168 30-31 23.02948567911711 25.48704523095717 28.02596548814092 23.457503601784808 32-33 22.952973978561804 25.652097893946667 28.020533670276997 23.374394457214525 34-35 23.770183959232384 25.762102332612113 27.43454164522857 23.033172062926933 36-37 23.751566719885407 25.370463972903206 27.62777626493979 23.250193042271594 38-39 23.30372793903886 25.589817366959316 27.863061238334964 23.24339345566686 40-41 23.294642553237125 25.511342627886364 28.015938848238235 23.178075970638275 42-43 23.46047926429287 25.59096759484074 27.261452470467063 23.687100670399317 44-45 23.329826302914643 25.69967121881235 27.26007051205986 23.710431966213147 46-47 23.491436938647574 26.145434615315576 26.930765592213035 23.43236285382381 48-49 23.939951323224975 25.939102743430496 26.709888285868814 23.411057647475708 50-51 23.315261457061343 26.146358885269226 26.858183795084695 23.680195862584736 52-53 23.859982123949013 26.151347767521816 26.422286036398322 23.566384072130845 54-55 23.273567795148196 25.80404891899831 26.686288040209437 24.236095245644055 56-57 23.57106872405448 26.38821573617729 26.55783856317623 23.482876976592003 58-59 23.841344916464934 25.641689762058405 26.712664729513484 23.804300591963177 60-61 23.60312651241121 25.82580566349334 27.170598586131554 23.40046923796389 62-63 23.367990967676057 26.019312816952535 26.942011426551872 23.67068478881954 64-65 23.06257152575137 25.89019472721894 26.91860360752053 24.128630139509163 66-67 23.255716365864203 26.120345231617666 26.6639017924125 23.96003661010564 68-69 23.07646757950501 25.9105420286571 26.987059963939668 24.02593042789822 70-71 23.181599891829894 25.973111335947447 26.889200240585236 23.956088531637427 72-73 23.39455978962779 25.88174955822769 26.739836905588945 23.983853746555575 74-75 23.060665274442144 26.435387680444837 26.344072485195376 24.15987455991764 76-77 23.188453221931322 26.07418941878808 26.56045516942519 24.176902189855408 78-79 23.22711511320344 26.136661931109533 26.597271405238892 24.038951550448136 80-81 23.059912088373217 26.541664772521962 26.498280116609372 23.900143022495445 82-83 23.261095595331902 26.22838346302867 26.364691785133136 24.145829156506295 84-85 23.03496412202707 26.069252180886526 26.334385505205688 24.561398191880716 86-87 23.17046502895185 25.83045051743657 26.632247910264784 24.366836543346793 88-89 22.87964920031976 25.94235993192936 26.436812811358585 24.741178056392293 90-91 22.913818912152514 26.01161474723629 26.606379016868058 24.468187323743138 92-93 23.055222704532422 25.720543461536664 26.600614518106834 24.623619315824076 94-95 22.802002079477056 25.57647206926617 26.715918256969278 24.9056075942875 96-97 22.906209057381442 25.464641896333873 26.727131580542434 24.902017465742247 98-99 22.66615254746608 25.57821508475157 26.356457585049387 25.399174782732963 100-101 22.731817625494664 24.901684918493306 27.150162314009123 25.216335142002904 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 572.0 1 391.5 2 286.0 3 365.5 4 320.5 5 324.0 6 578.5 7 1258.0 8 2019.0 9 2288.5 10 2037.0 11 1831.0 12 1936.0 13 2099.5 14 2267.0 15 2412.0 16 2552.5 17 2626.0 18 2729.5 19 2863.5 20 2996.5 21 3308.0 22 3855.5 23 4606.5 24 5379.0 25 6254.0 26 7512.0 27 9161.5 28 10862.5 29 13155.0 30 16169.5 31 19312.0 32 22926.5 33 25995.0 34 29019.5 35 31999.5 36 35562.5 37 39738.0 38 43311.5 39 45406.5 40 47214.0 41 54303.0 42 62062.0 43 67059.5 44 71484.5 45 75884.5 46 77961.0 47 82794.5 48 95680.0 49 98011.5 50 91503.0 51 88087.5 52 84821.5 53 85400.0 54 87236.5 55 82136.0 56 72912.5 57 65849.0 58 60002.5 59 52737.0 60 45253.0 61 38198.0 62 32213.5 63 26386.5 64 20763.0 65 16544.5 66 12592.5 67 10482.5 68 7946.0 69 5049.5 70 3825.5 71 3237.5 72 2788.0 73 2368.0 74 1899.5 75 1554.0 76 1089.5 77 562.0 78 321.0 79 215.0 80 126.5 81 87.5 82 59.5 83 30.5 84 15.5 85 8.0 86 4.0 87 2.5 88 3.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2868839082978594 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001398751381266989 16-17 7.460007366757275E-4 18-19 4.662504604223297E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 6.993756906334945E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0013288138122036395 36-37 0.0075532574588417405 38-39 0.0027741902395128615 40-41 0.003403628361083006 42-43 1.631876611478154E-4 44-45 0.0014220639042881057 46-47 2.5643775323228133E-4 48-49 4.6625046042232963E-4 50-51 4.662504604223297E-5 52-53 4.662504604223297E-5 54-55 0.0 56-57 5.361880294856791E-4 58-59 0.0019582519337737847 60-61 7.460007366757275E-4 62-63 0.0013288138122036395 64-65 2.3312523021116484E-5 66-67 3.263753222956308E-4 68-69 0.001445376427309222 70-71 0.0 72-73 0.0 74-75 4.662504604223297E-5 76-77 0.261589820819948 78-79 1.398751381266989E-4 80-81 1.1656261510558241E-4 82-83 0.0 84-85 0.0 86-87 0.10879954493955063 88-89 0.3807401259808744 90-91 0.28084596483539026 92-93 0.0 94-95 0.0 96-97 0.0 98-99 6.993756906334945E-5 100-101 3.4968784531674727E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2144770.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.777502220993036 #Duplication Level Percentage of deduplicated Percentage of total 1 67.21007312469274 25.39028686741184 2 15.17401701982301 11.464729233355 3 6.261196186791058 7.09597058557717 4 3.296579741236079 4.981461939849065 5 1.9822678666511724 3.74425643675089 6 1.2338006941847337 2.7965945078895915 7 0.862687985218602 2.281313809433382 8 0.6243723278011715 1.8869781600188276 9 0.46808784620169025 1.591487068455378 >10 2.5180120689384933 17.49054281162143 >50 0.20819578186807536 5.475643797450958 >100 0.148101517407299 10.811008689755317 >500 0.009517682522756341 2.486154361560067 >1k 0.0028429441301739723 1.907785631567148 >5k 2.4721253305860633E-4 0.5957860993040248 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7090 0.33057157643943175 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5669 0.2643173860134187 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4075 0.18999706262209934 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3936 0.18351618122222896 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 2339 0.1090559826927829 No Hit TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC 2200 0.10257510129291253 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 2180 0.10164260037206786 No Hit TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG 2166 0.1009898497274766 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 4.662504604223297E-5 0.0 0.0 0.0 0.0 6 4.662504604223297E-5 0.0 0.0 0.0 0.0 7 1.8650018416893187E-4 0.0 0.0 0.0 0.0 8 1.8650018416893187E-4 0.0 0.0 0.0 0.0 9 1.8650018416893187E-4 0.0 0.0 0.0 0.0 10-11 1.8650018416893187E-4 0.0 0.0 0.0 0.0 12-13 1.8650018416893187E-4 0.0 0.0 0.0 0.0 14-15 2.797502762533978E-4 0.0 0.0 6.993756906334945E-5 0.0 16-17 2.797502762533978E-4 0.0 0.0 1.398751381266989E-4 0.0 18-19 3.4968784531674727E-4 0.0 0.0 1.398751381266989E-4 0.0 20-21 4.196254143800967E-4 0.0 0.0 2.0981270719004836E-4 0.0 22-23 4.662504604223297E-4 0.0 0.0 4.662504604223297E-4 0.0 24-25 5.595005525067956E-4 0.0 0.0 8.15938305739077E-4 0.0 26-27 5.595005525067956E-4 0.0 0.0 0.001352126335224756 0.0 28-29 6.993756906334945E-4 0.0 0.0 0.0031938156538929585 0.0 30-31 7.926257827179605E-4 0.0 0.0 0.009255071639383244 0.0 32-33 8.15938305739077E-4 0.0 0.0 0.02088802062692037 0.0 34-35 8.392508287601934E-4 0.0 0.0 0.03727672431076526 0.0 36-37 9.325009208446594E-4 0.0 0.0 0.06438918858432373 0.0 38-39 0.0010257510129291253 0.0 0.0 0.10698116814390354 0.0 40-41 0.001049063535950242 0.0 0.0 0.15351296409405205 0.0 42-43 0.0011190011050135912 0.0 0.0 0.20356495102038913 0.0 44-45 0.0012588762431402902 0.0 0.0 0.2667185758845937 0.0 46-47 0.001492001473351455 0.0 0.0 0.34668052984702324 0.0 48-49 0.001492001473351455 0.0 0.0 0.43009273721657804 0.0 50-51 0.001492001473351455 0.0 0.0 0.52576733169524 0.0 52-53 0.001492001473351455 0.0 0.0 0.61764198492146 0.0 54-55 0.001492001473351455 0.0 0.0 0.7248096532495326 0.0 56-57 0.001538626519393688 0.0 0.0 0.8544505937699614 0.0 58-59 0.001538626519393688 0.0 0.0 0.9972864223203421 0.0 60-61 0.001538626519393688 0.0 0.0 1.160007833007735 0.0 62-63 0.001538626519393688 0.0 0.0 1.3214004298829245 0.0 64-65 0.001538626519393688 0.0 0.0 1.4955216643276437 0.0 66-67 0.001585251565435921 0.0 0.0 1.700182303930025 0.0 68-69 0.001585251565435921 0.0 0.0 1.930113718487297 0.0 70-71 0.001585251565435921 0.0 0.0 2.1862250963972825 0.0 72-73 0.0016085640884570375 0.0 0.0 2.4582123024846485 0.0 74-75 0.001631876611478154 0.0 0.0 2.745725648904078 0.0 76-77 0.001631876611478154 0.0 0.0 3.0516325759871687 0.0 78-79 0.001631876611478154 0.0 0.0 3.4032553607146685 0.0 80-81 0.0018183767956470858 0.0 0.0 3.792574495167314 0.0 82-83 0.0019582519337737847 0.0 0.0 4.19152170162768 0.0 84-85 0.0020048769798160174 0.0 0.0 4.596320351366347 0.0 86-87 0.0020048769798160174 0.0 0.0 5.026762776428242 0.0 88-89 0.0020048769798160174 0.0 0.0 5.496486802780717 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12745 0.0 26.023169 1 GGTATCA 9435 0.0 25.519716 1 GTACATG 10455 0.0 25.305677 1 TACATGG 10715 0.0 24.492565 2 ACATGGG 10680 0.0 23.550816 3 GAGTACT 6715 0.0 21.767374 12-13 TCAACGC 15240 0.0 21.64219 4 ATCAACG 15240 0.0 21.54877 3 TATCAAC 15385 0.0 21.40737 2 CAACGCA 15320 0.0 21.405266 5 AACGCAG 15585 0.0 21.0413 6 GTACTTT 7100 0.0 20.386509 14-15 CATGGGG 5595 0.0 20.356941 4 AGTACTT 6830 0.0 19.594301 12-13 GAGTGCA 1390 0.0 19.46083 9 CCAAGAC 1370 0.0 19.39853 3 ACGCAGA 17215 0.0 19.02144 7 GTACCGT 125 0.0060643614 18.982845 6 TACCGTA 125 0.0060643614 18.982845 7 AGAGTAC 12930 0.0 18.9021 10-11 >>END_MODULE