FastQCFastQC Report
Fri 27 May 2016
SRR939250_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939250_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2188529
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70120.32039785627697875No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51470.23518079952333282No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT38330.17514047106526803No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35710.16316895960711508No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG24190.11053086342470216No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG22970.1049563428220508No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG108100.025.3531171
GTATCAA127650.025.1912141
TACATGG109450.024.6097852
ACATGGG112200.023.7107493
GGTATCA95350.023.2636281
GAGTACT62300.021.37741312-13
CATGGGG57500.021.1128754
TCAACGC151550.020.9962544
ATCAACG151700.020.9754943
TATCAAC153150.020.9007592
CAACGCA152400.020.8791485
AACGCAG154900.020.530436
GTACTTT68450.019.7341414-15
AGTACTT66250.019.3874312-13
ATAACGC1751.0068985E-418.96863
AATTCGC5750.018.59978-79
AGAGTAC129450.018.4891210-11
ACGCAGA171250.018.4042457
ATGGGGA34800.018.2600025
CATGGGA55350.018.2489224
Produced by FastQC (version 0.11.5)