##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939250_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2188529 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.893796700889045 33.0 31.0 34.0 30.0 34.0 2 31.964933066913897 33.0 31.0 34.0 30.0 34.0 3 32.02140981453753 34.0 31.0 34.0 30.0 34.0 4 35.52002189598584 37.0 35.0 37.0 33.0 37.0 5 35.55929302284777 37.0 35.0 37.0 33.0 37.0 6 35.65171766058389 37.0 35.0 37.0 33.0 37.0 7 35.56773613692119 37.0 35.0 37.0 33.0 37.0 8 35.548808811763514 37.0 35.0 37.0 33.0 37.0 9 37.184017666661035 39.0 37.0 39.0 34.0 39.0 10-11 37.09305725443894 39.0 37.0 39.0 33.0 39.0 12-13 36.988762086314594 39.0 37.0 39.0 33.0 39.0 14-15 38.234221707822925 40.0 38.0 41.0 33.0 41.0 16-17 38.175002935761874 40.0 38.0 41.0 33.0 41.0 18-19 38.101310743426296 40.0 38.0 41.0 33.0 41.0 20-21 37.99766464140983 40.0 38.0 41.0 33.0 41.0 22-23 37.919831082887185 40.0 38.0 41.0 33.0 41.0 24-25 37.929058285268326 40.0 38.0 41.0 33.0 41.0 26-27 37.850820345538025 40.0 38.0 41.0 33.0 41.0 28-29 37.71514588109182 40.0 38.0 41.0 32.0 41.0 30-31 37.5435118748712 40.0 37.0 41.0 32.0 41.0 32-33 37.35469943510002 40.0 37.0 41.0 31.5 41.0 34-35 37.042884969767364 40.0 36.5 41.0 30.5 41.0 36-37 36.7966570239645 40.0 36.0 41.0 30.0 41.0 38-39 36.47977956883368 39.5 35.5 41.0 30.0 41.0 40-41 36.34784802942981 39.0 35.0 41.0 30.0 41.0 42-43 36.17072174963183 39.0 35.0 41.0 29.5 41.0 44-45 36.146516907018366 39.0 35.0 41.0 29.5 41.0 46-47 35.946174805085974 39.0 35.0 41.0 28.0 41.0 48-49 35.74071967974836 39.0 35.0 41.0 27.5 41.0 50-51 34.55918358861135 38.0 34.0 40.0 25.5 40.5 52-53 34.65930837562582 38.0 34.0 39.5 26.0 40.5 54-55 35.136148527161396 39.0 35.0 40.0 26.0 41.0 56-57 35.01438112083505 38.5 35.0 40.5 26.0 41.0 58-59 34.798029178503 38.0 34.5 40.0 26.0 41.0 60-61 34.48726930280567 38.0 34.0 40.0 25.5 41.0 62-63 34.12172376971016 37.0 34.0 40.0 24.5 41.0 64-65 33.7336601434114 36.5 33.5 40.0 23.0 41.0 66-67 33.3610596432581 36.0 33.0 39.0 22.5 41.0 68-69 32.94274007792448 35.5 33.0 39.0 22.0 40.5 70-71 32.52407576047656 35.0 32.5 38.5 20.5 40.0 72-73 32.09460304158638 35.0 32.0 37.0 20.0 39.5 74-75 31.651486226593295 35.0 32.0 37.0 18.0 39.0 76-77 31.225611586595377 35.0 31.0 36.0 17.0 39.0 78-79 30.81538101619855 35.0 31.0 36.0 12.5 37.5 80-81 30.371934756176408 34.5 31.0 35.5 7.5 37.0 82-83 29.96434659993082 34.0 30.0 35.0 6.0 37.0 84-85 29.576736474590923 34.0 30.0 35.0 2.0 36.0 86-87 29.269853403815986 34.0 29.5 35.0 2.0 36.0 88-89 28.88875610055887 34.0 29.0 35.0 2.0 35.5 90-91 28.517079965584188 34.0 29.0 35.0 2.0 35.0 92-93 28.12460355791493 34.0 29.0 35.0 2.0 35.0 94-95 27.66558725061445 34.0 27.0 35.0 2.0 35.0 96-97 27.103024908511607 33.5 26.5 35.0 2.0 35.0 98-99 26.38195038768049 33.0 25.0 35.0 2.0 35.0 100-101 23.924255972847515 31.0 12.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22969266340134098 1101 2 -0.020292325626869 1101 3 0.3051671449408957 1101 4 0.279432811221092 1101 5 0.33453413884134875 1101 6 0.3103121966657625 1101 7 0.3430276157682144 1101 8 0.2826134089432415 1101 9 0.39798177613528196 1101 10-11 0.4525005064871692 1101 12-13 0.4813292565696088 1101 14-15 0.4952647714111933 1101 16-17 0.4713502290155702 1101 18-19 0.44301416366311486 1101 20-21 0.5831175030127724 1101 22-23 0.6027087426339861 1101 24-25 0.5091554805686513 1101 26-27 0.4401383909201897 1101 28-29 0.3606928428401943 1101 30-31 0.45235386246734066 1101 32-33 0.5330883019457175 1101 34-35 0.4998403841114083 1101 36-37 0.46814086644830155 1101 38-39 0.699112278089693 1101 40-41 0.5348826002117448 1101 42-43 0.4426371180853721 1101 44-45 0.5025596453840322 1101 46-47 0.4860584929727949 1101 48-49 0.3613750691554358 1101 50-51 0.27985595685876063 1101 52-53 0.4419588408631725 1101 54-55 0.40740933561475856 1101 56-57 0.3898730004007831 1101 58-59 0.3858051642648803 1101 60-61 0.4873192475610395 1101 62-63 0.531646516436652 1101 64-65 0.5127770388244031 1101 66-67 0.4113569937679955 1101 68-69 0.408565009177984 1101 70-71 0.4515584489515234 1101 72-73 0.3769695928854091 1101 74-75 0.34804082682708426 1101 76-77 0.2560930136070745 1101 78-79 0.2010182101900604 1101 80-81 0.1586867887203134 1101 82-83 0.14726426603964882 1101 84-85 0.034006433309809125 1101 86-87 0.07005906518716998 1101 88-89 -0.003098586042884932 1101 90-91 0.0712406648251438 1101 92-93 0.14243382759878642 1101 94-95 0.18516249499269222 1101 96-97 0.25241395735183403 1101 98-99 0.0781774170059748 1101 100-101 -0.07563935832015645 1102 1 0.3923264564085791 1102 2 0.5285511982862978 1102 3 0.5400606573845081 1102 4 0.4415896372395878 1102 5 0.4003815618817086 1102 6 0.42352665022031744 1102 7 0.4294955479214764 1102 8 0.5508013474456774 1102 9 0.6147152121187034 1102 10-11 0.5694926373112139 1102 12-13 0.5880567252699436 1102 14-15 0.6200742508962875 1102 16-17 0.7168880807365028 1102 18-19 0.6432589859344588 1102 20-21 0.6917049091618495 1102 22-23 0.7559667311166365 1102 24-25 0.7676574301322461 1102 26-27 0.792537371208418 1102 28-29 0.8057501944835863 1102 30-31 0.726141227154578 1102 32-33 0.7437526187619454 1102 34-35 0.7177961129499622 1102 36-37 0.670535417386219 1102 38-39 0.8025533247360812 1102 40-41 0.7447637923070829 1102 42-43 0.6459774197436161 1102 44-45 0.756932037620274 1102 46-47 0.6584277641407397 1102 48-49 0.8009801633499194 1102 50-51 0.6512273642465018 1102 52-53 0.7721059862183424 1102 54-55 0.8701042042968581 1102 56-57 0.8551704046456337 1102 58-59 0.7833338985384088 1102 60-61 0.5422335792500164 1102 62-63 0.6887823296692659 1102 64-65 0.8319225093396057 1102 66-67 0.6421661516380937 1102 68-69 0.6476000291022643 1102 70-71 0.6715280384091997 1102 72-73 0.46521731852443793 1102 74-75 0.29835221924595245 1102 76-77 0.37485038990415376 1102 78-79 0.33693016146045096 1102 80-81 0.44120672794079496 1102 82-83 0.2968687549328912 1102 84-85 0.18458459991208898 1102 86-87 0.17760940585746354 1102 88-89 0.21462528384280688 1102 90-91 0.24287370263465036 1102 92-93 0.24767752889379224 1102 94-95 0.33206365137785454 1102 96-97 0.10455198266173582 1102 98-99 -0.031374834130136975 1102 100-101 -0.4030301748351661 1103 1 0.3680134426934991 1103 2 0.39623197101236585 1103 3 0.33831211439833453 1103 4 0.49073438813478276 1103 5 0.38077118589567505 1103 6 0.2858300908079059 1103 7 0.4103245195875971 1103 8 0.41064059878514314 1103 9 0.5387030125112844 1103 10-11 0.5095527758649752 1103 12-13 0.4657082629235276 1103 14-15 0.7094214606721536 1103 16-17 0.6801032056206964 1103 18-19 0.6778244981549193 1103 20-21 0.6959517679398957 1103 22-23 0.6711606267627204 1103 24-25 0.7090492622599882 1103 26-27 0.616590264823337 1103 28-29 0.6148597065624841 1103 30-31 0.6421261002480776 1103 32-33 0.6475656912505201 1103 34-35 0.6345816958755677 1103 36-37 0.7878987500580905 1103 38-39 0.8791353112295468 1103 40-41 0.8774843224566169 1103 42-43 0.8510543119024732 1103 44-45 0.9016379363301681 1103 46-47 0.8936433238223813 1103 48-49 0.7718580876471393 1103 50-51 0.6915461061651484 1103 52-53 0.6619664844911597 1103 54-55 0.7843859100679182 1103 56-57 0.8350263906310005 1103 58-59 0.7717382045498695 1103 60-61 0.7468677111023325 1103 62-63 0.6905121662536828 1103 64-65 0.729222214966569 1103 66-67 0.7229613759442799 1103 68-69 0.8835881126187601 1103 70-71 0.7413340100487815 1103 72-73 0.7204991595377166 1103 74-75 0.6613501981961996 1103 76-77 0.804736141819351 1103 78-79 0.6471150388513642 1103 80-81 0.5224419318721729 1103 82-83 0.6682382278547436 1103 84-85 0.5279510949770163 1103 86-87 0.48544397554070784 1103 88-89 0.3537986378374427 1103 90-91 0.504251934702733 1103 92-93 0.6114629462424617 1103 94-95 0.5814024179940063 1103 96-97 0.11465584110989013 1103 98-99 0.0725116736248026 1103 100-101 -0.17760880681578328 1104 1 0.25918536378540225 1104 2 0.32881676152117834 1104 3 0.29674357054921074 1104 4 0.30374287090400287 1104 5 0.3038757009499875 1104 6 0.16420473074880704 1104 7 0.24284857723671394 1104 8 0.2759990713909062 1104 9 0.2846056515399553 1104 10-11 0.2710179003301505 1104 12-13 0.19067479787838693 1104 14-15 0.31288620834570935 1104 16-17 0.46079350274071373 1104 18-19 0.20727101109721247 1104 20-21 0.36162419224692144 1104 22-23 0.4488265514143066 1104 24-25 0.49049438206485263 1104 26-27 0.3951531766293144 1104 28-29 0.39739281216824907 1104 30-31 0.4445174875849105 1104 32-33 0.44389804624772466 1104 34-35 0.5157590953193036 1104 36-37 0.6025794935274433 1104 38-39 0.6379212489696897 1104 40-41 0.6074095094890382 1104 42-43 0.5827084023225453 1104 44-45 0.6572221368626359 1104 46-47 0.5865726555626125 1104 48-49 0.6841157139590948 1104 50-51 0.6792812910657133 1104 52-53 0.7983025826156265 1104 54-55 0.8098292599437187 1104 56-57 0.8082929012927096 1104 58-59 0.8513364256968003 1104 60-61 0.8407916983509196 1104 62-63 0.7536162103184694 1104 64-65 0.7789101224344748 1104 66-67 0.7402150856065077 1104 68-69 0.864456518145694 1104 70-71 0.7780992044187158 1104 72-73 0.6663894041052245 1104 74-75 0.6132072603122758 1104 76-77 0.702150905109928 1104 78-79 0.6598427870752381 1104 80-81 0.5736881949421324 1104 82-83 0.4942584159530874 1104 84-85 0.3533073117908465 1104 86-87 0.3540371546208405 1104 88-89 0.46570723612304477 1104 90-91 0.5888520645966828 1104 92-93 0.4649395925987392 1104 94-95 0.36352402748915225 1104 96-97 0.4567756905915523 1104 98-99 0.35680243776812404 1104 100-101 0.17719664972180027 1105 1 0.20722638594512333 1105 2 0.23380876074534385 1105 3 0.3256559499314591 1105 4 0.31148616722364153 1105 5 0.32166894150664405 1105 6 0.24381196538973882 1105 7 0.282152267291508 1105 8 0.28705565869633887 1105 9 0.3427465621737156 1105 10-11 0.37321496186339687 1105 12-13 0.37831941796275714 1105 14-15 0.4160554853665204 1105 16-17 0.39367111511525366 1105 18-19 0.3197818000222057 1105 20-21 0.39682154471746145 1105 22-23 0.44000193811847765 1105 24-25 0.4010381923737256 1105 26-27 0.4484429501831073 1105 28-29 0.35664001189671524 1105 30-31 0.44592513923597465 1105 32-33 0.5144947101666446 1105 34-35 0.3986793784982723 1105 36-37 0.4044628276689366 1105 38-39 0.4668690257238026 1105 40-41 0.3393607290012355 1105 42-43 0.23829900504765789 1105 44-45 0.37558173536915973 1105 46-47 0.4155124315408827 1105 48-49 0.4952940100362824 1105 50-51 0.5456954888061034 1105 52-53 0.5035253921001868 1105 54-55 0.5127929412703978 1105 56-57 0.4675037338582939 1105 58-59 0.7085899775563789 1105 60-61 0.5180787080003384 1105 62-63 0.5503686226736022 1105 64-65 0.3908419461264714 1105 66-67 0.3253760709141744 1105 68-69 0.34918570400615323 1105 70-71 0.24887552212225472 1105 72-73 0.4225829743908278 1105 74-75 0.4124748256519517 1105 76-77 0.26128197236493733 1105 78-79 0.18794223914331454 1105 80-81 0.31898107182716373 1105 82-83 0.18080741464386918 1105 84-85 0.1412787514455971 1105 86-87 0.2319545284267832 1105 88-89 0.21802891580107087 1105 90-91 0.3133596532113394 1105 92-93 0.30360758464645343 1105 94-95 0.2455341618052067 1105 96-97 0.18556393672450966 1105 98-99 0.019319045439168292 1105 100-101 -0.1977073409682042 1106 1 0.10709037229765173 1106 2 0.18174550287269042 1106 3 0.18670625192785906 1106 4 0.19319256217038827 1106 5 0.15607347836864705 1106 6 0.1547313254339997 1106 7 0.11996959404700647 1106 8 0.238904061112315 1106 9 0.24940569814125269 1106 10-11 0.22232142135899124 1106 12-13 0.21339434073467345 1106 14-15 0.34468266965347993 1106 16-17 0.37522374548087356 1106 18-19 0.42416858595054663 1106 20-21 0.44858165352837887 1106 22-23 0.4451402570820093 1106 24-25 0.34820680319026565 1106 26-27 0.4029805037817056 1106 28-29 0.503881009637503 1106 30-31 0.3409968345654093 1106 32-33 0.3033176157870088 1106 34-35 0.4307004490870554 1106 36-37 0.48086930099643155 1106 38-39 0.5890786711661917 1106 40-41 0.6286760501317588 1106 42-43 0.4238177011608997 1106 44-45 0.5951047654765844 1106 46-47 0.5522845279953117 1106 48-49 0.37345275410009293 1106 50-51 0.44035614628224806 1106 52-53 0.4548852980432869 1106 54-55 0.3385186547657142 1106 56-57 0.4620555804164539 1106 58-59 0.47780962807046023 1106 60-61 0.3921372169219097 1106 62-63 0.43971229919165467 1106 64-65 0.4917728327510318 1106 66-67 0.37161148979009795 1106 68-69 0.3634897290879877 1106 70-71 0.37948850566230163 1106 72-73 0.3716981940874504 1106 74-75 0.27123687092835524 1106 76-77 0.2835326524331734 1106 78-79 0.27174462745075445 1106 80-81 0.2708506831508757 1106 82-83 0.31681645148281845 1106 84-85 0.2484111688000148 1106 86-87 0.35289279206272894 1106 88-89 0.38230735187465825 1106 90-91 0.3036319110378862 1106 92-93 0.4048835770850161 1106 94-95 0.27271914036576916 1106 96-97 0.1847213437050108 1106 98-99 0.07245934697451162 1106 100-101 0.11501961739778821 1107 1 0.10170020480918751 1107 2 0.11381330904258036 1107 3 0.15289570204925695 1107 4 0.1902501674510546 1107 5 0.188102507940215 1107 6 0.04797252028200205 1107 7 0.19762898569505438 1107 8 0.04282005689976387 1107 9 0.19936604025660642 1107 10-11 0.2915372087222323 1107 12-13 0.29445109116760193 1107 14-15 0.29726362681855534 1107 16-17 0.2835892457650857 1107 18-19 0.3244108295292776 1107 20-21 0.41234260901901365 1107 22-23 0.29786310134549154 1107 24-25 0.19942297831948963 1107 26-27 0.3249219995889874 1107 28-29 0.2762266853858222 1107 30-31 0.4065687232941926 1107 32-33 0.444728378936901 1107 34-35 0.49405887704850215 1107 36-37 0.5413967067979399 1107 38-39 0.742793182867473 1107 40-41 0.5575260596055855 1107 42-43 0.6206396725102792 1107 44-45 0.6236464348972675 1107 46-47 0.44538053092117735 1107 48-49 0.5416701542173072 1107 50-51 0.4691516261646811 1107 52-53 0.4415303952271401 1107 54-55 0.5094863291832965 1107 56-57 0.4680074577766504 1107 58-59 0.43282557935295074 1107 60-61 0.35782400335538256 1107 62-63 0.5214243961683991 1107 64-65 0.5273300921268103 1107 66-67 0.4622570760878588 1107 68-69 0.5165226759772636 1107 70-71 0.41962371701679047 1107 72-73 0.435708223125836 1107 74-75 0.4546966688493974 1107 76-77 0.2557711417321542 1107 78-79 0.3052337281421238 1107 80-81 0.4416259066671202 1107 82-83 0.45637453045488385 1107 84-85 0.40806026261735084 1107 86-87 0.5431080903120531 1107 88-89 0.5114685655821845 1107 90-91 0.6008995929464014 1107 92-93 0.6755190065578773 1107 94-95 0.4427420758423892 1107 96-97 0.5006244859314002 1107 98-99 0.7782740741502963 1107 100-101 0.5612590584860015 1108 1 0.10834692809965318 1108 2 0.099413037145748 1108 3 0.07271178692724334 1108 4 0.20109397746956148 1108 5 0.26806967511507906 1108 6 0.14382449041183065 1108 7 0.11915789236839203 1108 8 0.2407853195142664 1108 9 0.24303754527611687 1108 10-11 0.17258009980638178 1108 12-13 0.1994706016891783 1108 14-15 0.24050868987104934 1108 16-17 0.26232646452982067 1108 18-19 0.15482479887034373 1108 20-21 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2316 70-71 -0.8464927547167989 2316 72-73 -1.0472131988479383 2316 74-75 -1.1528054710944637 2316 76-77 -0.8303812614626658 2316 78-79 -0.9253105079045945 2316 80-81 -0.7174849989856291 2316 82-83 -0.5709941268717387 2316 84-85 -0.597660268284681 2316 86-87 -0.3029724499603077 2316 88-89 -0.5093499484728596 2316 90-91 -0.41067997511005316 2316 92-93 -0.5623266912419673 2316 94-95 -0.6389288707120109 2316 96-97 -0.5483866863124796 2316 98-99 -0.5505721910352861 2316 100-101 -0.21557102774416137 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6591.0 3 1800.0 4 1961.0 5 2215.0 6 2381.0 7 2967.0 8 3650.0 9 4413.0 10 5435.0 11 6819.0 12 8513.0 13 11900.0 14 11091.0 15 11760.0 16 14812.0 17 18665.0 18 16170.0 19 12291.0 20 12567.0 21 13804.0 22 14508.0 23 16139.0 24 18116.0 25 20971.0 26 24396.0 27 28576.0 28 33552.0 29 40364.0 30 48749.0 31 59502.0 32 74309.0 33 96821.0 34 132283.0 35 193921.0 36 303033.0 37 459503.0 38 399769.0 39 54207.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.13054067218704 18.074918699615413 14.16012819273266 23.634412435464885 2 15.545525820088685 19.8305618146106 39.618491916899266 25.00542044840145 3 19.452736113570023 23.883346622877504 29.069013677937388 27.59490358561509 4 13.228191705205736 15.865851555233895 35.61161035136012 35.294346388200246 5 14.768945000194442 36.73519433966149 32.79166962143169 15.704191038712379 6 33.286274846858 34.46875647187532 17.10874670662981 15.136221974636877 7 30.098752867280513 31.937150034039313 19.74088059631205 18.22321650236812 8 27.1292674395477 34.035037984870065 19.43284462439216 19.40284995119007 9 27.46308960501042 16.751107963391142 19.66397835327812 36.121824078320316 10-11 26.087564793489506 25.703747306819675 26.347161659399127 21.861526240291692 12-13 26.786565041858506 23.006154183793374 27.097909132058906 23.109371642289208 14-15 23.44829414997362 24.44032153793963 25.48371507810307 26.62766923398368 16-17 23.47569374037432 27.853218534247354 25.696495058599332 22.974592666778985 18-19 23.21741527901674 26.1070984243454 28.175530876401577 22.499955420236287 20-21 23.99818381593441 25.350765408186454 27.77657369468629 22.874477081192847 22-23 23.678547355539926 25.194348083362065 27.89146505530003 23.235639505797977 24-25 23.667740092156205 25.471968411427078 27.858855714555865 23.001435781860856 26-27 23.2991739740073 25.80251932897164 27.84684448192502 23.051462215096034 28-29 22.98050852611934 25.981284563263035 28.18645104083638 22.851755869781243 30-31 23.090133288946365 25.424198885326142 27.938914263033034 23.54675356269446 32-33 22.95118546960966 25.714282448236798 27.914417107433575 23.420114974719965 34-35 23.817385366041336 25.75652023364428 27.313507554324403 23.112586845989984 36-37 23.745278435039545 25.378556407811136 27.485360941614132 23.390804215535184 38-39 23.3286974645152 25.62859882526916 27.69903728737718 23.343666422838456 40-41 23.3034392929577 25.518435727813195 27.8709727103143 23.307152268914805 42-43 23.462344184929055 25.58351990411874 27.177101104936153 23.777034806016054 44-45 23.350384857274594 25.75986125832772 27.17774924011008 23.712004644287603 46-47 23.4895172320494 26.092490492879833 26.86259875424338 23.555393520827387 48-49 23.91118903665288 25.941615159218667 26.648104570247416 23.49909123388104 50-51 23.37896343637578 26.12846396181866 26.810312942908904 23.682259658896655 52-53 23.830440399478476 26.138879169087698 26.446715336501047 23.583965094932775 54-55 23.286424372483744 25.80144165955925 26.70050714868719 24.211626819269817 56-57 23.539924839002115 26.347219054539284 26.60884992670714 23.504006179751457 58-59 23.844756167266905 25.6083385014661 26.707397511687315 23.839507819579683 60-61 23.60371228507769 25.71860692177325 27.250776714464596 23.426904078684462 62-63 23.410237659125244 25.951381549839187 27.005430833102178 23.632949957933395 64-65 23.147607988171107 25.87125256372155 26.91864059467333 24.06249885343401 66-67 23.307789611954977 26.09346635392884 26.65930228398295 23.939441750133238 68-69 23.098533274649945 25.89205666539988 27.069730404270892 23.93967965567928 70-71 23.23519522654683 25.934505382198726 26.951841167455715 23.878458223798734 72-73 23.46528582291555 25.774328267304348 26.838373220400236 23.92201268937987 74-75 23.150078703465887 26.43411382465822 26.483372287574024 23.93243518430187 76-77 23.33647227039157 26.052077006449153 26.61506795259907 23.99638277056021 78-79 23.324076646649484 26.200733015882623 26.664805242796614 23.810385094671286 80-81 23.227996015895158 26.619390762057478 26.529897405861767 23.622715816185597 82-83 23.403922640331096 26.340010766306047 26.376968358615198 23.879098234747655 84-85 23.25891458933574 26.146282677809147 26.38897741712267 24.205825315732444 86-87 23.476255478914062 25.925357003495407 26.59561980337124 24.002767714219292 88-89 23.227267026012154 26.041273749580668 26.472795596133707 24.258663628273474 90-91 23.243175553204434 26.15164295656725 26.675989089233564 23.929192400994747 92-93 23.426273037883934 25.846211216704834 26.6207648157772 24.106750929634032 94-95 23.27026024129322 25.746375625008888 26.713433683352072 24.26993045034582 96-97 23.425575418827844 25.67293986241094 26.717506654113393 24.18397806464782 98-99 23.249763964289983 25.865408849166243 26.31915697240429 24.565670214139484 100-101 23.366692858123848 25.292968682828025 26.98072417277374 24.359614286274383 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1778.0 1 1038.0 2 388.0 3 489.5 4 433.5 5 435.5 6 714.5 7 1427.0 8 2217.5 9 2503.0 10 2313.0 11 2133.5 12 2259.5 13 2453.5 14 2668.5 15 2894.0 16 3030.0 17 3062.0 18 3131.0 19 3255.5 20 3417.5 21 3730.5 22 4198.0 23 4919.5 24 5630.0 25 6530.0 26 7879.0 27 9548.0 28 11141.0 29 13266.5 30 16488.0 31 19729.0 32 23359.0 33 26574.0 34 29343.5 35 32390.0 36 36113.0 37 40354.5 38 44420.0 39 46588.5 40 48492.5 41 55843.5 42 63271.5 43 68158.0 44 72524.0 45 76597.0 46 79184.0 47 83876.0 48 96770.5 49 99516.0 50 92259.0 51 88576.0 52 85723.0 53 86765.0 54 88609.0 55 83502.0 56 73883.5 57 66779.0 58 60890.0 59 53283.0 60 46008.0 61 38847.5 62 32810.5 63 26909.5 64 21243.0 65 17005.0 66 13056.5 67 11158.5 68 8619.0 69 5503.5 70 4236.5 71 3463.5 72 2876.0 73 2427.0 74 1874.0 75 1518.5 76 1086.0 77 642.5 78 408.0 79 284.0 80 186.5 81 124.5 82 99.5 83 75.0 84 55.0 85 39.0 86 26.0 87 13.5 88 11.5 89 12.5 90 7.5 91 6.0 92 8.0 93 7.5 94 6.5 95 4.5 96 2.5 97 1.5 98 2.0 99 3.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2828383813968195 2 0.10797206708250152 3 0.1188012587450292 4 0.12117728391992978 5 0.1276656603590814 6 0.05391749435351325 7 0.06177665454741518 8 0.06735117515006656 9 0.06433545088961581 10-11 0.059012240642002 12-13 0.06392421576319071 14-15 0.0589893942460895 16-17 0.06147965140055261 18-19 0.06570623464436615 20-21 0.06972720032496714 22-23 0.07909422264909444 24-25 0.08752454274080901 26-27 0.0875016963448965 28-29 0.08124178386486997 30-31 0.0777234388943441 32-33 0.06975004672087964 34-35 0.2380594454083085 36-37 0.36442286120037704 38-39 0.33577348072609503 40-41 0.319415461252741 42-43 0.3426045531039342 44-45 0.35574123075362496 46-47 0.36218391440095155 48-49 0.37011161378259094 50-51 0.3649711747022772 52-53 0.36490263551453966 54-55 0.35224573217901156 56-57 0.3295135682460685 58-59 0.31482333567432735 60-61 0.2713237978569167 62-63 0.3410509981818838 64-65 0.370340077741716 66-67 0.37536628484246726 68-69 0.3869494075701076 70-71 0.38710933234149514 72-73 0.3711625479945662 74-75 0.374361043422317 76-77 0.35889403338955067 78-79 0.33826373788055814 80-81 0.33636748701982017 82-83 0.3495955502531609 84-85 0.3566779329860376 86-87 0.3503723277141861 88-89 0.3651082530777522 90-91 0.3874977210720077 92-93 0.382448667575344 94-95 0.3819917396570939 96-97 0.3877718778229578 98-99 0.37671422220130507 100-101 0.3560610802964 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2188529.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.07558895880689 #Duplication Level Percentage of deduplicated Percentage of total 1 68.64094888281616 26.821855042874 2 14.614065366043791 11.421064225213259 3 5.999088247045996 7.032537194075362 4 3.143095015384086 4.912731555184935 5 1.9007801382068887 3.713705169081827 6 1.160813482385578 2.721568229732404 7 0.7655242503535375 2.093931766136953 8 0.5926518991434936 1.8526577605269947 9 0.4518520245652068 1.589074558390326 >10 2.3818977145415756 17.09555642365711 >50 0.19959336169740236 5.394853211758167 >100 0.1374720316417051 10.437142009488582 >500 0.00928066642113125 2.4770201825172875 >1k 0.0027019661732407435 1.8781468448389977 >5k 2.349535802818038E-4 0.5581558265238641 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7012 0.32039785627697875 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5147 0.23518079952333282 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3833 0.17514047106526803 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3571 0.16316895960711508 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 2419 0.11053086342470216 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 2297 0.1049563428220508 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 9.138558365002246E-5 0.0 0.0 0.0 0.0 6 9.138558365002246E-5 0.0 0.0 0.0 0.0 7 2.2846395912505614E-4 0.0 0.0 0.0 0.0 8 2.2846395912505614E-4 0.0 0.0 0.0 0.0 9 2.2846395912505614E-4 0.0 0.0 4.569279182501123E-5 0.0 10-11 2.2846395912505614E-4 0.0 0.0 6.853918773751685E-5 0.0 12-13 2.2846395912505614E-4 0.0 0.0 9.138558365002246E-5 0.0 14-15 3.198495427750786E-4 0.0 0.0 9.138558365002246E-5 0.0 16-17 3.198495427750786E-4 0.0 0.0 9.138558365002246E-5 0.0 18-19 3.8838873051259544E-4 0.0 0.0 9.138558365002246E-5 0.0 20-21 4.5692791825011227E-4 0.0 0.0 1.8277116730004491E-4 0.0 22-23 4.5692791825011227E-4 0.0 0.0 3.198495427750786E-4 0.0 24-25 5.94006293725146E-4 0.0 0.0 7.082382732876741E-4 0.0 26-27 5.94006293725146E-4 0.0 0.0 0.0012793981711003144 0.0 28-29 9.138558365002245E-4 0.0 0.0 0.003061417052275752 0.0 30-31 9.595486283252358E-4 0.0 0.0 0.008201856132589516 4.569279182501123E-5 32-33 9.595486283252358E-4 0.0 0.0 0.018231423938179482 4.569279182501123E-5 34-35 0.001005241420150247 0.0 0.0 0.034246747472845916 4.569279182501123E-5 36-37 0.0011423197956252806 0.0 0.0 0.06289612794712796 4.569279182501123E-5 38-39 0.0011423197956252806 0.0 0.0 0.10242039287576267 4.569279182501123E-5 40-41 0.0011651661915377863 0.0 0.0 0.1498038179982993 4.569279182501123E-5 42-43 0.0012108589833627974 0.0 0.0 0.19910634037748642 4.569279182501123E-5 44-45 0.0012793981711003144 0.0 0.0 0.2627107065979021 4.569279182501123E-5 46-47 0.0013479373588378312 0.0 0.0 0.3390176689456708 4.569279182501123E-5 48-49 0.0014164765465753482 0.0 0.0 0.4203279919982783 4.569279182501123E-5 50-51 0.0014164765465753482 0.0 0.0 0.5124675067134135 4.569279182501123E-5 52-53 0.0014393229424878536 0.0 0.0 0.6043328646775985 4.569279182501123E-5 54-55 0.0014621693384003593 0.0 0.0 0.7091292827282618 6.853918773751685E-5 56-57 0.0015535549220503818 0.0 0.0 0.8323398958844046 9.138558365002246E-5 58-59 0.0015535549220503818 0.0 0.0 0.9696695817144758 9.138558365002246E-5 60-61 0.0015535549220503818 0.0 0.0 1.124568146001264 9.138558365002246E-5 62-63 0.0015764013179628874 0.0 0.0 1.2823225097771151 9.138558365002246E-5 64-65 0.0016449405057004042 0.0 0.0 1.456183582671283 9.138558365002246E-5 66-67 0.0016449405057004042 0.0 0.0 1.6544902991918318 9.138558365002246E-5 68-69 0.0016906332975254155 0.0 0.0 1.8841194245084254 9.138558365002246E-5 70-71 0.0016906332975254155 0.0 0.0 2.1409129145649883 9.138558365002246E-5 72-73 0.0017363260893504266 0.0 0.0 2.4034408499955906 9.138558365002246E-5 74-75 0.001827711673000449 0.0 0.0 2.6856623787027725 9.138558365002246E-5 76-77 0.001827711673000449 0.0 0.0 2.984127694903746 9.138558365002246E-5 78-79 0.001827711673000449 0.0 0.0 3.325475696232492 9.138558365002246E-5 80-81 0.0022389467994255504 0.0 0.0 3.708861066040249 9.138558365002246E-5 82-83 0.0022846395912505613 0.0 0.0 4.1059542733955094 9.138558365002246E-5 84-85 0.002376025174900584 0.0 0.0 4.521895757378585 9.138558365002246E-5 86-87 0.002467410758550606 0.0 0.0 4.955931586924368 9.138558365002246E-5 88-89 0.002467410758550606 0.0 0.0 5.426864345868847 1.370783754750337E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10810 0.0 25.353117 1 GTATCAA 12765 0.0 25.191214 1 TACATGG 10945 0.0 24.609785 2 ACATGGG 11220 0.0 23.710749 3 GGTATCA 9535 0.0 23.263628 1 GAGTACT 6230 0.0 21.377413 12-13 CATGGGG 5750 0.0 21.112875 4 TCAACGC 15155 0.0 20.996254 4 ATCAACG 15170 0.0 20.975494 3 TATCAAC 15315 0.0 20.900759 2 CAACGCA 15240 0.0 20.879148 5 AACGCAG 15490 0.0 20.53043 6 GTACTTT 6845 0.0 19.73414 14-15 AGTACTT 6625 0.0 19.38743 12-13 ATAACGC 175 1.0068985E-4 18.9686 3 AATTCGC 575 0.0 18.599 78-79 AGAGTAC 12945 0.0 18.48912 10-11 ACGCAGA 17125 0.0 18.404245 7 ATGGGGA 3480 0.0 18.260002 5 CATGGGA 5535 0.0 18.248922 4 >>END_MODULE