FastQCFastQC Report
Fri 27 May 2016
SRR939250_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939250_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2188529
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72650.33195813260870655No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57770.2639672583730899No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT42490.1941486724644727No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40540.18523857805859553No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG24570.1122671895140526No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG23210.10605296982585107No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA101300.025.1807581
GTACATG112350.024.906791
TACATGG113650.024.4268682
GTATCAA131650.023.6412471
ACATGGG116100.023.012173
CATGGGG60800.022.166454
GAGTACT68800.021.10689212-13
GGACCGT2751.2751116E-920.7076076
AGTACTT71400.019.83980812-13
GTACTTT74150.019.61600914-15
TCAACGC161050.019.418074
ATCAACG160950.019.400653
CAACGCA161650.019.2872835
TATCAAC162300.019.2685152
AACGCAG165650.018.7928986
TACGGGT2302.0783646E-618.5693234
AGAGTAC138850.018.45606810-11
ATGGGGA33450.018.159145
AGTACAT87450.018.1245842
ATGCACT18550.017.6517014
Produced by FastQC (version 0.11.5)