##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939250_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2188529 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.29577995082542 33.0 31.0 34.0 31.0 34.0 2 32.495452881821535 34.0 31.0 34.0 31.0 34.0 3 32.58443456769364 34.0 31.0 34.0 31.0 34.0 4 36.11717962156316 37.0 35.0 37.0 35.0 37.0 5 36.05373152469078 37.0 35.0 37.0 35.0 37.0 6 36.0877095985477 37.0 35.0 37.0 35.0 37.0 7 36.0297839324953 37.0 35.0 37.0 35.0 37.0 8 36.00899051371949 37.0 35.0 37.0 35.0 37.0 9 37.715248461409466 39.0 37.0 39.0 35.0 39.0 10-11 37.633376345481366 39.0 37.0 39.0 35.0 39.0 12-13 37.54884239596551 39.0 37.0 39.0 35.0 39.0 14-15 38.85700920572677 40.0 38.0 41.0 35.0 41.0 16-17 38.801506628424846 40.0 38.0 41.0 35.0 41.0 18-19 38.90375430254751 40.0 38.0 41.0 35.0 41.0 20-21 38.90074657452563 40.0 38.0 41.0 35.0 41.0 22-23 38.843394809938545 40.0 38.0 41.0 35.0 41.0 24-25 38.767113892482115 40.0 38.0 41.0 34.0 41.0 26-27 38.60170781378725 40.0 38.0 41.0 34.0 41.0 28-29 38.451210607673005 40.0 38.0 41.0 34.0 41.0 30-31 38.263315679161664 40.0 38.0 41.0 34.0 41.0 32-33 38.07322772510668 40.0 37.5 41.0 33.0 41.0 34-35 38.02985361400283 40.0 37.0 41.0 33.0 41.0 36-37 37.9465922087393 40.0 37.0 41.0 33.0 41.0 38-39 37.84275442546112 40.0 37.0 41.0 33.0 41.0 40-41 37.663476929024014 40.0 37.0 41.0 33.0 41.0 42-43 37.47636151954121 40.0 37.0 41.0 32.5 41.0 44-45 37.245387198433285 40.0 36.5 41.0 31.5 41.0 46-47 36.93836956238643 40.0 36.0 41.0 31.0 41.0 48-49 36.61534299979576 39.5 36.0 41.0 30.5 41.0 50-51 36.35784104300194 39.0 35.0 41.0 30.0 41.0 52-53 36.11883164445159 39.0 35.0 41.0 29.5 41.0 54-55 35.81407374542444 39.0 35.0 40.0 29.0 41.0 56-57 35.5511882638978 38.0 35.0 40.0 28.0 41.0 58-59 35.238007355625626 38.0 34.5 40.0 28.0 41.0 60-61 34.87982087511749 37.5 34.0 40.0 27.5 41.0 62-63 34.493237923737816 37.0 34.0 40.0 26.5 41.0 64-65 34.058778750475774 36.0 33.5 39.0 26.0 41.0 66-67 33.6691272996611 36.0 33.0 39.0 26.0 40.0 68-69 33.27646743543266 35.0 33.0 38.5 25.5 40.0 70-71 32.87527467079486 35.0 32.5 38.0 24.5 40.0 72-73 32.383192317762294 35.0 32.0 37.0 23.5 39.0 74-75 31.891481218663312 35.0 31.5 36.5 22.0 39.0 76-77 29.91488872205943 33.0 29.5 35.0 19.5 37.0 78-79 30.907154760115127 34.0 31.0 35.0 20.0 37.0 80-81 30.96771552947208 34.0 31.0 35.0 20.0 37.0 82-83 30.68457420486546 34.0 31.0 35.0 19.5 36.5 84-85 30.340327224359378 34.0 31.0 35.0 17.5 36.0 86-87 29.83068878685181 34.0 30.0 35.0 10.5 36.0 88-89 29.35740102141667 34.0 29.5 35.0 6.5 35.0 90-91 28.963006658810553 34.0 29.0 35.0 2.0 35.0 92-93 28.6315155065343 34.0 29.0 35.0 2.0 35.0 94-95 28.23536996768149 34.0 29.0 35.0 2.0 35.0 96-97 27.683173263868106 33.0 28.0 35.0 2.0 35.0 98-99 26.932754603663007 33.0 26.5 35.0 2.0 35.0 100-101 23.549258885762995 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.234477521661141 1101 2 0.17596983017243417 1101 3 0.1688885046678692 1101 4 0.06163780869312063 1101 5 0.12663830173801216 1101 6 0.16969473248501288 1101 7 0.23948518378315242 1101 8 0.23944916488775192 1101 9 0.2629994974811325 1101 10-11 0.26376446456728786 1101 12-13 0.26445761403577706 1101 14-15 0.3418794468347457 1101 16-17 0.3502887059908346 1101 18-19 0.2862848508948943 1101 20-21 0.33968785642842647 1101 22-23 0.32777555009505477 1101 24-25 0.29736657118417753 1101 26-27 0.30810586534462914 1101 28-29 0.310086669073101 1101 30-31 0.2914269196142314 1101 32-33 0.26099918851501513 1101 34-35 0.3357677474019738 1101 36-37 0.3361749970313923 1101 38-39 0.3525380847243653 1101 40-41 0.4113656781466588 1101 42-43 0.37273846846738223 1101 44-45 0.4161840770045373 1101 46-47 0.38082016409443753 1101 48-49 0.28748783167889513 1101 50-51 0.39763959284800166 1101 52-53 0.30160201824791244 1101 54-55 0.24984806479456978 1101 56-57 0.35733457230300303 1101 58-59 0.32296280969536184 1101 60-61 0.3649401393204954 1101 62-63 0.48454853925317565 1101 64-65 0.376740210808002 1101 66-67 0.3573601160182349 1101 68-69 0.44568200138109404 1101 70-71 0.43553523147934214 1101 72-73 0.2601978311002142 1101 74-75 0.24931755717191706 1101 76-77 0.3796264500870308 1101 78-79 0.7021609024646089 1101 80-81 0.6575358998924195 1101 82-83 0.6743721513550653 1101 84-85 0.6231170917421878 1101 86-87 0.7735857060062479 1101 88-89 0.9334861015998754 1101 90-91 1.1094072000632664 1101 92-93 0.9338577014348814 1101 94-95 1.0583428137555408 1101 96-97 0.7632053786116479 1101 98-99 0.6568523086108193 1101 100-101 0.5907710678648819 1102 1 0.20553139269248533 1102 2 0.23921211979062917 1102 3 0.23270829843419705 1102 4 0.18622231355281116 1102 5 0.22334647613647718 1102 6 0.22341482820054637 1102 7 0.290049919452251 1102 8 0.30137018320707654 1102 9 0.3714980568381918 1102 10-11 0.32399089918968116 1102 12-13 0.31883899486559386 1102 14-15 0.37538767672347007 1102 16-17 0.46161334671337073 1102 18-19 0.437287118046811 1102 20-21 0.38873868952296675 1102 22-23 0.3362588600173453 1102 24-25 0.43882625123699626 1102 26-27 0.3993002130260308 1102 28-29 0.36117253534101934 1102 30-31 0.415973106047538 1102 32-33 0.32888437248113433 1102 34-35 0.43872039647818895 1102 36-37 0.43249375230342224 1102 38-39 0.3590188347159895 1102 40-41 0.4817502626567176 1102 42-43 0.46768256642953787 1102 44-45 0.40492339558082335 1102 46-47 0.37028194737424514 1102 48-49 0.4062622239551459 1102 50-51 0.22012526110707853 1102 52-53 0.4355473899405382 1102 54-55 0.3284392871991244 1102 56-57 0.42294766842406517 1102 58-59 0.47961918797278 1102 60-61 0.33775029320989347 1102 62-63 0.36734221492029207 1102 64-65 0.3628286736364075 1102 66-67 0.3813161467280608 1102 68-69 0.42280979761954995 1102 70-71 0.3170404987692024 1102 72-73 0.5693110180164567 1102 74-75 0.5794732754631546 1102 76-77 0.6494501307706209 1102 78-79 0.6957370455941891 1102 80-81 0.7086320277050255 1102 82-83 0.5588443039907389 1102 84-85 0.48321145119188813 1102 86-87 0.5421295512486459 1102 88-89 0.5873048380374257 1102 90-91 0.6633184982318028 1102 92-93 0.7866868872423609 1102 94-95 0.8254722544390027 1102 96-97 0.6695986586767368 1102 98-99 0.5922263990677656 1102 100-101 0.4332968834433615 1103 1 0.16079857193141578 1103 2 0.18326885580159313 1103 3 0.1808923239254625 1103 4 0.15774940054895126 1103 5 0.14444565419234578 1103 6 0.1239329196925425 1103 7 0.22087870152646616 1103 8 0.1520129629783753 1103 9 0.2590922998166576 1103 10-11 0.2532825964543832 1103 12-13 0.23867890345701426 1103 14-15 0.2678153758486985 1103 16-17 0.2874628132649306 1103 18-19 0.22781927725851148 1103 20-21 0.28280673785121024 1103 22-23 0.2709364842537596 1103 24-25 0.3060130738409086 1103 26-27 0.28641074384545817 1103 28-29 0.3528613315967064 1103 30-31 0.395244643372493 1103 32-33 0.32344814045602277 1103 34-35 0.3603150413915017 1103 36-37 0.4009673983959985 1103 38-39 0.38068299666286975 1103 40-41 0.37259701804396883 1103 42-43 0.4662214447785189 1103 44-45 0.5551608278491074 1103 46-47 0.5086877069236735 1103 48-49 0.30085127085133223 1103 50-51 0.35334732505516087 1103 52-53 0.2959300116933079 1103 54-55 0.3399923862052887 1103 56-57 0.3838036379781258 1103 58-59 0.1867008249851736 1103 60-61 0.1100351158719377 1103 62-63 0.29870867965051673 1103 64-65 0.4534764839130432 1103 66-67 0.4052250768861896 1103 68-69 0.41211024139101227 1103 70-71 0.5101517399263145 1103 72-73 0.42459450215962136 1103 74-75 0.5097449101119587 1103 76-77 0.5175215892253462 1103 78-79 0.6671028530724534 1103 80-81 0.7116253047759074 1103 82-83 0.6053273108678638 1103 84-85 0.6462676980557234 1103 86-87 0.4525065484414199 1103 88-89 0.5978514567921067 1103 90-91 0.7096751105024452 1103 92-93 0.641681938893516 1103 94-95 0.6711631463172658 1103 96-97 0.5784389502438145 1103 98-99 0.5126588455744105 1103 100-101 0.6558444172949969 1104 1 0.17156514456543448 1104 2 0.1410746395529472 1104 3 0.11055450049200033 1104 4 0.10506178559032264 1104 5 0.12831277565310728 1104 6 0.11021919208313591 1104 7 0.1970944742219629 1104 8 0.09920834430636916 1104 9 0.27551818341778755 1104 10-11 0.18377693714340637 1104 12-13 0.1971221343459959 1104 14-15 0.21759822488912306 1104 16-17 0.13374150024529996 1104 18-19 0.0438999751162541 1104 20-21 0.15038989229115174 1104 22-23 0.24113610944525732 1104 24-25 0.15583161892947572 1104 26-27 0.2201114076927979 1104 28-29 0.279911747306933 1104 30-31 0.17392753356252655 1104 32-33 0.17707513162620359 1104 34-35 0.2529614075833777 1104 36-37 0.27464081921770145 1104 38-39 0.3910225801098193 1104 40-41 0.43054595782176364 1104 42-43 0.402724620239475 1104 44-45 0.5507797576617577 1104 46-47 0.3382730378310015 1104 48-49 0.45683209414102777 1104 50-51 0.3363622033663418 1104 52-53 0.31404389696025703 1104 54-55 0.3147696577381822 1104 56-57 0.29199327819171117 1104 58-59 0.3158478877457682 1104 60-61 0.21656042784052687 1104 62-63 0.23544920904274136 1104 64-65 0.28928098359822485 1104 66-67 0.19015155294256658 1104 68-69 0.34747039964349113 1104 70-71 0.2960156090804702 1104 72-73 0.4214620499898487 1104 74-75 0.41035657677036497 1104 76-77 0.5069667139879925 1104 78-79 0.5832238329289758 1104 80-81 0.5473351107778015 1104 82-83 0.5855161280892247 1104 84-85 0.5718776294895669 1104 86-87 0.6144057269365355 1104 88-89 0.9358028010953632 1104 90-91 0.9626375106650578 1104 92-93 0.7621052841090048 1104 94-95 0.7570670495809786 1104 96-97 0.7204539425840402 1104 98-99 0.6868106925970103 1104 100-101 0.604427978273268 1105 1 0.09960034797909856 1105 2 0.14791942450179363 1105 3 0.18016852367000524 1105 4 0.11682001669151987 1105 5 0.10425953412666189 1105 6 0.17503808361201578 1105 7 0.19750421092370374 1105 8 0.19543246300878536 1105 9 0.19046508735998913 1105 10-11 0.14387197666239615 1105 12-13 0.18815277974191247 1105 14-15 0.17968570003822748 1105 16-17 0.19255496094451274 1105 18-19 0.19039393816721173 1105 20-21 0.1729794767357049 1105 22-23 0.2796385096780014 1105 24-25 0.27831767818370423 1105 26-27 0.2190419706564768 1105 28-29 0.24158221232172394 1105 30-31 0.3158334153450042 1105 32-33 0.3408619230726515 1105 34-35 0.39531169609620775 1105 36-37 0.4252803963888212 1105 38-39 0.30487434755393394 1105 40-41 0.4159556945235039 1105 42-43 0.43845123615374604 1105 44-45 0.4749753160189272 1105 46-47 0.3603013557284882 1105 48-49 0.3101895106177821 1105 50-51 0.38687813218368206 1105 52-53 0.40537469267378157 1105 54-55 0.2790052199745219 1105 56-57 0.3022349986009587 1105 58-59 0.35216947044469293 1105 60-61 0.3858401155678166 1105 62-63 0.36424788042905476 1105 64-65 0.24795309725772796 1105 66-67 0.23782041780219032 1105 68-69 0.3394279227972561 1105 70-71 0.3739228000808055 1105 72-73 0.37883755306893363 1105 74-75 0.47775050587864243 1105 76-77 0.4220067178671556 1105 78-79 0.4398271399163214 1105 80-81 0.4928563734456972 1105 82-83 0.46182874022131415 1105 84-85 0.31206722363202744 1105 86-87 0.44176450112069787 1105 88-89 0.7011347120685514 1105 90-91 0.7353347419117249 1105 92-93 0.734269372747903 1105 94-95 0.7314934181985393 1105 96-97 0.689626225956971 1105 98-99 0.6979346803776458 1105 100-101 0.33338836231050806 1106 1 0.026618191125393764 1106 2 0.08845932952277025 1106 3 0.06060561159475952 1106 4 0.06520076269180208 1106 5 0.11318802322251287 1106 6 0.119091290306649 1106 7 0.05197652407783693 1106 8 0.03542093642849409 1106 9 0.13327711188855318 1106 10-11 0.11144908948456589 1106 12-13 0.12573288947496053 1106 14-15 0.05470460362990792 1106 16-17 0.03557999667691547 1106 18-19 0.05788506270038596 1106 20-21 0.03159697869521949 1106 22-23 0.07826315811730211 1106 24-25 0.13704030255551203 1106 26-27 0.24865329436896388 1106 28-29 0.1841146020123503 1106 30-31 0.1561349506854981 1106 32-33 0.2688115749699236 1106 34-35 0.24169488573149778 1106 36-37 0.3197651582496732 1106 38-39 0.3585158248004703 1106 40-41 0.37687413244133694 1106 42-43 0.3040642196937924 1106 44-45 0.34192607446791357 1106 46-47 0.5155369590297099 1106 48-49 0.4562326661343974 1106 50-51 0.3797906684924115 1106 52-53 0.22601441788010845 1106 54-55 0.2746371071062512 1106 56-57 0.40502350890572103 1106 58-59 0.3168771563684345 1106 60-61 0.24178713940479213 1106 62-63 0.4101815334328833 1106 64-65 0.35483285197209824 1106 66-67 0.47396662094664066 1106 68-69 0.3938382229494053 1106 70-71 0.4770400484555637 1106 72-73 0.42647967580396084 1106 74-75 0.5408956485777132 1106 76-77 0.5100451244326933 1106 78-79 0.6308910432774724 1106 80-81 0.5180486368050019 1106 82-83 0.44775432461893416 1106 84-85 0.4195472826481179 1106 86-87 0.6076015613816601 1106 88-89 0.725845625434772 1106 90-91 0.6128346266459204 1106 92-93 0.5625154882903445 1106 94-95 0.617935393393342 1106 96-97 0.6630657575167511 1106 98-99 0.6992427167094242 1106 100-101 0.5883021434899085 1107 1 0.007067514958464471 1107 2 0.05184915330956841 1107 3 0.04038229363987256 1107 4 0.022947710136293153 1107 5 0.03992955516253005 1107 6 0.04055744230210223 1107 7 0.03669347694224001 1107 8 0.07142655022238387 1107 9 0.024583001965709173 1107 10-11 0.11975917889105858 1107 12-13 0.10523163584515771 1107 14-15 0.1730656534181776 1107 16-17 0.0605417708825442 1107 18-19 0.06165950187180158 1107 20-21 0.13341099643551502 1107 22-23 0.07132479087648136 1107 24-25 0.12358801760039029 1107 26-27 0.17970431644077678 1107 28-29 0.08433393214661322 1107 30-31 0.10396235957150424 1107 32-33 0.10361136874892907 1107 34-35 0.17620771065075047 1107 36-37 0.23412285474347527 1107 38-39 0.13013047354177587 1107 40-41 0.18768575308283175 1107 42-43 0.2032320650809183 1107 44-45 0.18183016848353617 1107 46-47 0.14832114082144443 1107 48-49 0.2811162261786393 1107 50-51 0.2020739932332063 1107 52-53 0.00847208308824321 1107 54-55 -0.03621395109831127 1107 56-57 0.1024754898170599 1107 58-59 0.1491147203167671 1107 60-61 0.1991160266486105 1107 62-63 0.2303818277825158 1107 64-65 0.29952453395112144 1107 66-67 0.2851511358646164 1107 68-69 0.1698691076286849 1107 70-71 0.06104315939629146 1107 72-73 0.14578337147817422 1107 74-75 0.25059034896238686 1107 76-77 0.2167159111129351 1107 78-79 0.43106939359759977 1107 80-81 0.5151043693522297 1107 82-83 0.5220163574821015 1107 84-85 0.6164512136257727 1107 86-87 0.5002095520047725 1107 88-89 0.5067848343040389 1107 90-91 0.5351795312687102 1107 92-93 0.39498516102467107 1107 94-95 0.516744995889141 1107 96-97 0.3990372214655302 1107 98-99 0.4188920969448162 1107 100-101 0.4061952302393266 1108 1 0.05079481013004994 1108 2 0.061733629218807096 1108 3 0.04344113301232966 1108 4 0.04703238291422451 1108 5 -0.0016764316979234195 1108 6 0.026716995724378023 1108 7 -0.01140532934088867 1108 8 0.1097159087318289 1108 9 0.15557507271019233 1108 10-11 0.11250261152444807 1108 12-13 0.008230837468843788 1108 14-15 0.010102939836478697 1108 16-17 0.04775877212980362 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-0.4331275163324193 2316 62-63 -0.46902807323227336 2316 64-65 -0.6543156521015234 2316 66-67 -0.5526052231756537 2316 68-69 -0.44554472322266747 2316 70-71 -0.3515077338577015 2316 72-73 -0.3942070800522117 2316 74-75 -0.5518156516094486 2316 76-77 -0.5999620231041689 2316 78-79 -0.6574469683998494 2316 80-81 -0.5947093553258433 2316 82-83 -0.5839505453132325 2316 84-85 -0.7666513798703996 2316 86-87 -0.8793575246582144 2316 88-89 -1.0435940315123489 2316 90-91 -1.0695031379917204 2316 92-93 -0.8464962590964156 2316 94-95 -0.7628804226357779 2316 96-97 -0.6445640123535092 2316 98-99 -0.8231492269077414 2316 100-101 -0.21307985208504832 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 9.0 4 24.0 5 60.0 6 116.0 7 304.0 8 652.0 9 1159.0 10 1913.0 11 3025.0 12 4269.0 13 6135.0 14 7415.0 15 9612.0 16 12429.0 17 17918.0 18 17201.0 19 11812.0 20 11413.0 21 12308.0 22 12621.0 23 13959.0 24 16367.0 25 19188.0 26 22941.0 27 27789.0 28 34118.0 29 42039.0 30 51288.0 31 62548.0 32 77661.0 33 100206.0 34 136737.0 35 199986.0 36 317413.0 37 507177.0 38 399599.0 39 29116.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.25921025080399 18.695135624027223 14.594639917417359 23.451014207751424 2 16.00467711417121 20.414305682035742 38.68274078159348 24.89827642219957 3 19.505430359844446 23.979302993014944 29.362919111421416 27.152347535719194 4 13.517664147927672 16.373372251407222 35.33725164254163 34.77171195812347 5 14.816618833929091 36.88143954226789 32.714668162953295 15.58727346084973 6 33.435426261201016 34.76823930594477 16.702360352547306 15.09397408030691 7 29.980365807352793 32.16873068622805 19.619022640321422 18.23188086609773 8 27.24674884362967 34.22572878860641 19.165704452625484 19.36181791513843 9 27.208823826414914 16.72822247272026 19.572096143117136 36.49085755774769 10-11 26.12259193275483 25.892208876373125 26.11637771306663 21.86882147780541 12-13 26.754020622984665 23.067160636208158 27.070009124850525 23.108809615956655 14-15 23.31968156952484 24.565703930209224 25.42358088978489 26.691033610481046 16-17 23.37474788921473 27.96350687815795 25.6896663593055 22.972078873321813 18-19 23.11845079503173 26.15667875545629 28.181531978785753 22.543338470726226 20-21 23.944370853664722 25.319289806075222 27.872603013256846 22.863736327003206 22-23 23.594181297117835 25.1798582518212 28.062365177706123 23.163595273354844 24-25 23.621785140106695 25.477986692885196 27.983574351442076 22.91665381556603 26-27 23.260112844207733 25.80468210247731 27.996179125100983 22.939025928213976 28-29 22.91756243577307 26.025768906877634 28.288704422011314 22.767964235337985 30-31 23.08315311334691 25.41131508881079 28.064695510089198 23.440836287753097 32-33 22.969149597743506 25.665435550545595 27.98420765728944 23.381207194421457 34-35 23.80436580575765 25.733117595827572 27.4052051166527 23.057311481762078 36-37 23.73484626205512 25.30746217906707 27.69387598408463 23.263815574793174 38-39 23.307519788556498 25.624912606825305 27.844516460989972 23.223051143628226 40-41 23.310211224882213 25.47353022429062 28.02596512923725 23.19029342158991 42-43 23.439126457328612 25.61149083677645 27.280296030655883 23.669086675239054 44-45 23.3151086592219 25.723955017017236 27.21688413132823 23.744052192432637 46-47 23.496866386904806 26.13733980099866 26.95697747527105 23.408816336825485 48-49 23.880035402775107 25.933857856121623 26.73784537467861 23.448261366424664 50-51 23.308327191460563 26.121609537730595 26.887809117448295 23.682254153360546 52-53 23.83518792759886 26.17100344569343 26.399970025528564 23.593838601179147 54-55 23.281048594741033 25.856088724435455 26.625555338768642 24.23730734205487 56-57 23.564755139182527 26.342282875849488 26.597157268649397 23.495804716318585 58-59 23.822114407048787 25.672361080712573 26.728143552786154 23.777380959452486 60-61 23.59898962390541 25.829475273384595 27.201995712115924 23.36953939059407 62-63 23.365196151787313 26.022728389101836 26.953595767688 23.65847969142286 64-65 23.132105947949018 25.895927741369483 26.89411030932899 24.077856001352508 66-67 23.25265052462179 26.14626079892019 26.666861622578452 23.93422705387957 68-69 23.063691444446473 25.914819147186225 26.991597061027544 24.029892347339754 70-71 23.259144214937113 25.952049516375958 26.86346099673822 23.925345271948707 72-73 23.3980906810008 25.854900711848007 26.747052472231346 23.99995613491985 74-75 23.155210646054954 26.44968378303417 26.30876721304584 24.08633835786503 76-77 23.219029988655613 26.084987169440783 26.583943183728202 24.112039658175398 78-79 23.21447419705199 26.163875370168732 26.612030272388438 24.00962016039084 80-81 23.069176297491094 26.566979018758257 26.48070998319627 23.883134700554372 82-83 23.25959582897919 26.243289442360602 26.401706351617914 24.095408377042297 84-85 23.02523749970871 26.03241720808817 26.417036283275202 24.525309008927916 86-87 23.225759235584302 25.859224377871648 26.601570272316234 24.31344611422782 88-89 22.914047102177 25.94166751501578 26.442514488427143 24.701770894380076 90-91 22.907735039462334 26.057820310755282 26.61170539375894 24.422739256023444 92-93 23.0451367105485 25.746631641618638 26.613880830457354 24.594350817375506 94-95 22.84907416101732 25.574741201679572 26.70106877749136 24.875115859811743 96-97 22.91383847323933 25.543892724291066 26.715889988206694 24.826378814262913 98-99 22.673243077884734 25.66792580770006 26.353294838679314 25.305536275735896 100-101 22.6033014990489 25.10567639058524 27.135357622796402 25.155664487569457 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 530.0 1 379.5 2 292.0 3 381.5 4 340.5 5 342.5 6 614.5 7 1310.0 8 2111.0 9 2421.0 10 2151.5 11 1917.0 12 2019.5 13 2216.0 14 2405.5 15 2510.5 16 2568.0 17 2632.0 18 2781.0 19 2927.5 20 3078.0 21 3448.0 22 4005.5 23 4769.0 24 5492.5 25 6290.0 26 7536.5 27 9237.0 28 11017.5 29 13364.5 30 16443.5 31 19594.5 32 23186.5 33 26437.5 34 29367.5 35 32560.5 36 36218.5 37 40489.5 38 44589.0 39 46448.5 40 48362.0 41 55883.0 42 63452.5 43 68511.5 44 72789.0 45 77155.0 46 79535.0 47 84284.0 48 97837.0 49 100527.0 50 93301.5 51 89761.0 52 86554.5 53 87340.5 54 89209.5 55 83928.5 56 74811.0 57 67452.5 58 60966.0 59 53588.0 60 46093.0 61 38583.0 62 32637.0 63 26978.5 64 21271.5 65 16678.0 66 12548.0 67 10627.0 68 8152.5 69 5142.0 70 3935.0 71 3296.5 72 2784.0 73 2333.5 74 1907.5 75 1616.0 76 1085.0 77 557.5 78 339.0 79 209.0 80 127.5 81 92.5 82 58.5 83 26.0 84 15.0 85 12.0 86 5.5 87 2.5 88 1.5 89 1.5 90 2.0 91 1.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27360843744816726 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0020561756321255055 16-17 3.198495427750786E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.2846395912505614E-5 26-27 0.0011423197956252806 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0030614170522757526 36-37 0.02079022028038011 38-39 0.006534069230976606 40-41 0.016609329828391584 42-43 0.0048891287252762015 44-45 5.711598978126403E-4 46-47 4.569279182501123E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.569279182501123E-4 60-61 0.0016906332975254153 62-63 0.001485015734312865 64-65 6.853918773751685E-5 66-67 0.0 68-69 4.1123512642510105E-4 70-71 2.2846395912505614E-5 72-73 0.0 74-75 0.0 76-77 0.2316624545528069 78-79 0.0 80-81 0.0012108589833627976 82-83 0.0 84-85 0.0 86-87 0.10436233652832563 88-89 0.35745471044706284 90-91 0.3583228734917381 92-93 0.0 94-95 2.2846395912505614E-5 96-97 0.0 98-99 0.0 100-101 3.6554233460008983E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2188529.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.5143870907793 #Duplication Level Percentage of deduplicated Percentage of total 1 66.97067833830472 25.12363950915232 2 15.36508114683795 11.528232036474279 3 6.254309036491741 7.038797105409303 4 3.354566277273727 5.033779913892842 5 1.9387078960841722 3.6364719234825973 6 1.2568136300659463 2.8289155811556848 7 0.8636122441937674 2.2678518817515125 8 0.605093813422172 1.8159778834364122 9 0.4494583044296788 1.5175037532186268 >10 2.5711035999402316 17.767387158105734 >50 0.20779304598305512 5.421175452134939 >100 0.1499718576627334 10.966158091274671 >500 0.009516601201763593 2.473480550842951 >1k 0.0030501926928729467 1.9836910322446568 >5k 2.440154154298357E-4 0.5969381274235075 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7265 0.33195813260870655 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5777 0.2639672583730899 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4249 0.1941486724644727 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4054 0.18523857805859553 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 2457 0.1122671895140526 No Hit TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG 2321 0.10605296982585107 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 4.569279182501123E-5 0.0 0.0 0.0 0.0 6 9.138558365002246E-5 0.0 0.0 0.0 0.0 7 1.8277116730004491E-4 0.0 0.0 0.0 0.0 8 1.8277116730004491E-4 0.0 0.0 0.0 0.0 9 1.8277116730004491E-4 0.0 0.0 4.569279182501123E-5 0.0 10-11 1.8277116730004491E-4 0.0 0.0 6.853918773751685E-5 0.0 12-13 2.0561756321255052E-4 0.0 0.0 9.138558365002246E-5 0.0 14-15 2.513103550375618E-4 0.0 0.0 9.138558365002246E-5 0.0 16-17 2.97003146862573E-4 0.0 0.0 9.138558365002246E-5 0.0 18-19 4.3408152233760666E-4 0.0 0.0 9.138558365002246E-5 0.0 20-21 5.483135019001348E-4 0.0 0.0 1.8277116730004491E-4 0.0 22-23 5.483135019001348E-4 0.0 0.0 3.198495427750786E-4 0.0 24-25 8.681630446752133E-4 0.0 0.0 7.082382732876741E-4 0.0 26-27 9.138558365002245E-4 0.0 0.0 0.0012793981711003144 0.0 28-29 0.0011423197956252806 0.0 0.0 0.0031756490318382803 0.0 30-31 0.001188012587450292 0.0 0.0 0.008476012883539583 0.0 32-33 0.001188012587450292 0.0 0.0 0.018825430231904625 0.0 34-35 0.0012793981711003144 0.0 0.0 0.03493213935022108 0.0 36-37 0.0014621693384003593 0.0 0.0 0.06369575180406566 0.0 38-39 0.0015535549220503818 0.0 0.0 0.10367694465095048 0.0 40-41 0.0015535549220503818 0.0 0.0 0.15154014408764974 0.0 42-43 0.0015535549220503818 0.0 0.0 0.20093405205048687 0.0 44-45 0.001599247713875393 0.0 0.0 0.26492680700141513 0.0 46-47 0.001599247713875393 0.0 0.0 0.3414850797042214 0.0 48-49 0.001599247713875393 0.0 0.0 0.4229553275282164 0.0 50-51 0.001599247713875393 0.0 0.0 0.5152090742229142 0.0 52-53 0.001599247713875393 0.0 0.0 0.6079425952317744 0.0 54-55 0.001599247713875393 0.0 0.0 0.7130588628252128 0.0 56-57 0.001599247713875393 0.0 0.0 0.8368406358791681 0.0 58-59 0.001599247713875393 0.0 0.0 0.9747643280029645 0.0 60-61 0.001599247713875393 0.0 0.0 1.1301883593957402 0.0 62-63 0.0016449405057004042 0.0 0.0 1.2889479645917419 0.0 64-65 0.0016449405057004042 0.0 0.0 1.4633345045918973 0.0 66-67 0.0016449405057004042 0.0 0.0 1.6623494593857335 0.0 68-69 0.0016906332975254155 0.0 0.0 1.8922527414532775 0.0 70-71 0.0018048652770879434 0.0 0.0 2.148977692322103 0.0 72-73 0.0018505580689129547 0.0 0.0 2.4116883989200053 0.0 74-75 0.0018734044648254604 0.0 0.0 2.6945267803168247 0.0 76-77 0.0018734044648254604 0.0 0.0 2.994385726668461 0.0 78-79 0.0019190972566504715 0.0 0.0 3.335139721703482 0.0 80-81 0.0022846395912505613 0.0 0.0 3.7190048658254016 0.0 82-83 0.0022846395912505613 0.0 0.0 4.116075226784749 0.0 84-85 0.0023303323830755726 0.0 0.0 4.532770641832939 0.0 86-87 0.00239887157081309 0.0 0.0 4.969251035741358 0.0 88-89 0.0024217179667255953 0.0 0.0 5.441051957730512 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10130 0.0 25.180758 1 GTACATG 11235 0.0 24.90679 1 TACATGG 11365 0.0 24.426868 2 GTATCAA 13165 0.0 23.641247 1 ACATGGG 11610 0.0 23.01217 3 CATGGGG 6080 0.0 22.16645 4 GAGTACT 6880 0.0 21.106892 12-13 GGACCGT 275 1.2751116E-9 20.707607 6 AGTACTT 7140 0.0 19.839808 12-13 GTACTTT 7415 0.0 19.616009 14-15 TCAACGC 16105 0.0 19.41807 4 ATCAACG 16095 0.0 19.40065 3 CAACGCA 16165 0.0 19.287283 5 TATCAAC 16230 0.0 19.268515 2 AACGCAG 16565 0.0 18.792898 6 TACGGGT 230 2.0783646E-6 18.569323 4 AGAGTAC 13885 0.0 18.456068 10-11 ATGGGGA 3345 0.0 18.15914 5 AGTACAT 8745 0.0 18.124584 2 ATGCACT 1855 0.0 17.651701 4 >>END_MODULE