FastQCFastQC Report
Fri 27 May 2016
SRR939249_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939249_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2052983
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68020.3313227630233665No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53760.26186286004316645No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36410.17735168776361032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56550.038.0157661
GTATCAA86450.032.9370271
GCGTATC700.00843043227.1181551
ATCAACG107800.026.2605463
TCAACGC109550.025.8404184
CAACGCA110350.025.5671425
TATCAAC111100.025.5658912
GTACATG97100.025.365671
AACGCAG113950.024.8678516
TACATGG96850.024.6762452
ACATGGG100300.023.2128643
GTGTACG2503.8744474E-1022.7792511
ACGCAGA130300.021.783817
CGCAGAG130900.021.7568938
GAGTACT61300.021.33224312-13
CATGGGG53550.020.0118184
TGTACGC1200.004812289519.7577272
GCAGAGT140550.019.756399
GTACACG2651.8762876E-819.6990361
ATTCGCG2900.019.66707480-81
Produced by FastQC (version 0.11.5)