##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939249_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2052983 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.807645265450322 33.0 31.0 34.0 30.0 34.0 2 31.873066167620483 33.0 31.0 34.0 30.0 34.0 3 31.921969154152762 34.0 31.0 34.0 30.0 34.0 4 35.408069623567265 37.0 35.0 37.0 33.0 37.0 5 35.43926082193569 37.0 35.0 37.0 33.0 37.0 6 35.52452212219975 37.0 35.0 37.0 33.0 37.0 7 35.45507537081408 37.0 35.0 37.0 33.0 37.0 8 35.43434699654113 37.0 35.0 37.0 33.0 37.0 9 37.04964775645975 39.0 37.0 39.0 33.0 39.0 10-11 36.93994567904362 39.0 37.0 39.0 33.0 39.0 12-13 36.83472171956612 39.0 37.0 39.0 33.0 39.0 14-15 38.052077391775775 40.0 38.0 41.0 33.0 41.0 16-17 37.97160936062305 40.0 38.0 41.0 33.0 41.0 18-19 37.888994940532875 40.0 38.0 41.0 33.0 41.0 20-21 37.8581512852274 40.0 38.0 41.0 32.5 41.0 22-23 37.86293481241685 40.0 38.0 41.0 32.0 41.0 24-25 37.77690146484409 40.0 38.0 41.0 32.0 41.0 26-27 37.684945759414475 40.0 38.0 41.0 32.0 41.0 28-29 37.53493307056122 40.0 37.5 41.0 32.0 41.0 30-31 37.342209360720474 40.0 37.0 41.0 31.5 41.0 32-33 37.12308382485388 40.0 37.0 41.0 31.0 41.0 34-35 36.831809128473054 40.0 36.0 41.0 30.0 41.0 36-37 36.59345766623494 40.0 36.0 41.0 30.0 41.0 38-39 35.99419576294592 38.5 35.5 40.5 28.5 41.0 40-41 35.96695077358166 39.0 35.0 40.0 29.0 41.0 42-43 36.143510686644746 39.0 35.0 41.0 29.5 41.0 44-45 36.04790565728017 39.0 35.0 41.0 28.5 41.0 46-47 35.76654117447636 39.0 35.0 41.0 27.0 41.0 48-49 35.51904545726876 39.0 35.0 41.0 26.0 41.0 50-51 34.38179955703481 38.0 34.0 40.0 24.5 40.5 52-53 34.48225143608106 38.0 34.0 39.5 24.0 40.5 54-55 34.95982796740158 39.0 35.0 40.0 25.0 41.0 56-57 34.872008925548826 39.0 35.0 40.5 25.0 41.0 58-59 34.648129575354496 38.0 34.0 40.0 24.0 41.0 60-61 34.34493417626936 38.0 34.0 40.0 23.0 41.0 62-63 33.95402519163578 37.5 34.0 40.0 22.5 41.0 64-65 33.55784071275797 37.0 33.0 40.0 22.0 41.0 66-67 33.19601964555966 36.0 33.0 39.0 20.5 41.0 68-69 32.79014292860681 36.0 32.5 39.0 19.5 41.0 70-71 32.3769337106055 35.0 32.0 39.0 18.0 40.0 72-73 31.942475412606925 35.0 32.0 38.0 16.5 40.0 74-75 31.47398517182071 35.0 31.0 37.0 12.0 39.0 76-77 31.01214330561919 35.0 31.0 36.5 8.0 39.0 78-79 30.610240562147858 35.0 31.0 36.0 7.0 39.0 80-81 30.203925945806663 34.5 30.0 36.0 2.0 37.5 82-83 29.781083671905712 34.0 30.0 35.0 2.0 37.0 84-85 29.36805053914231 34.0 29.5 35.0 2.0 36.5 86-87 28.92908027002659 34.0 29.0 35.0 2.0 36.0 88-89 28.4988553241795 34.0 29.0 35.0 2.0 36.0 90-91 28.08649267918926 34.0 28.5 35.0 2.0 35.0 92-93 27.631647217731466 34.0 27.0 35.0 2.0 35.0 94-95 27.142252517434386 33.5 26.0 35.0 2.0 35.0 96-97 26.525292708220185 33.0 25.0 35.0 2.0 35.0 98-99 25.711481293318066 33.0 23.0 35.0 2.0 35.0 100-101 22.986183032202412 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3336650092333713 1101 2 0.37044401851710873 1101 3 0.510778587135043 1101 4 0.3611468308736434 1101 5 0.2944802207486177 1101 6 0.35832958058718134 1101 7 0.37587713821427826 1101 8 0.2133222347692012 1101 9 0.21950967529942744 1101 10-11 0.4212359529424461 1101 12-13 0.32089570102667864 1101 14-15 0.5097663923110076 1101 16-17 0.5262718025996023 1101 18-19 0.6347364618174751 1101 20-21 0.5923917334471653 1101 22-23 0.645713253360924 1101 24-25 0.6047270550588166 1101 26-27 0.6244570155240297 1101 28-29 0.6285322609820128 1101 30-31 0.5703761164777887 1101 32-33 0.5255097552664907 1101 34-35 0.5528721474338383 1101 36-37 0.4213710112104323 1101 38-39 1.0019405847171825 1101 40-41 0.8776657501497098 1101 42-43 0.5594238171237507 1101 44-45 0.5155849202878144 1101 46-47 0.6676611243166874 1101 48-49 0.5901107670076655 1101 50-51 0.6288105011250948 1101 52-53 0.5994504185880416 1101 54-55 0.5095778363298678 1101 56-57 0.611404013826153 1101 58-59 0.6486839027056917 1101 60-61 0.5102209511683782 1101 62-63 0.6871731780420092 1101 64-65 0.6309597839055385 1101 66-67 0.4340725045698619 1101 68-69 0.5923111089369968 1101 70-71 0.6367291483687225 1101 72-73 0.4560722946033344 1101 74-75 0.4440775142501785 1101 76-77 0.4891469044149801 1101 78-79 0.3720913276816624 1101 80-81 0.5118766678722793 1101 82-83 0.3960396843926368 1101 84-85 0.49457292406595954 1101 86-87 0.4015545560206739 1101 88-89 0.29202931704308455 1101 90-91 0.3543337603883323 1101 92-93 0.44988215947767074 1101 94-95 0.615299807530338 1101 96-97 0.4830958091216857 1101 98-99 0.5487048873242486 1101 100-101 0.6314604556518617 1102 1 0.3405568073968297 1102 2 0.2570422716839609 1102 3 0.41176869756179 1102 4 0.3913203207289442 1102 5 0.28119505245636134 1102 6 0.38307571092337156 1102 7 0.3394619110504422 1102 8 0.42391600698441323 1102 9 0.5253155979736022 1102 10-11 0.5930660630693936 1102 12-13 0.5959012791541909 1102 14-15 0.7687195031765057 1102 16-17 0.6585822486018813 1102 18-19 0.6602140907875267 1102 20-21 0.7088826386489799 1102 22-23 0.6518630308412057 1102 24-25 0.7408479242896391 1102 26-27 0.7198366539460537 1102 28-29 0.7137860845217645 1102 30-31 0.8017608815881658 1102 32-33 0.6303583732457625 1102 34-35 0.5915780103197221 1102 36-37 0.5708515679046755 1102 38-39 0.9428945915423839 1102 40-41 0.8879421888886085 1102 42-43 0.7372444326146308 1102 44-45 0.7490308539625801 1102 46-47 0.6432623657455352 1102 48-49 0.538776571154564 1102 50-51 0.6465032549789029 1102 52-53 0.6978964416281528 1102 54-55 0.7194551002280463 1102 56-57 0.7048021918107992 1102 58-59 0.7027379762378061 1102 60-61 0.6683770095914028 1102 62-63 0.6350328202462165 1102 64-65 0.780617629923853 1102 66-67 0.7039950680524143 1102 68-69 0.559893828099753 1102 70-71 0.530444342726831 1102 72-73 0.5658995715603901 1102 74-75 0.43741302117945224 1102 76-77 0.4349324056652293 1102 78-79 0.43329362027765583 1102 80-81 0.2777296486102969 1102 82-83 0.287786390702923 1102 84-85 0.32261193694206725 1102 86-87 0.33427296926104333 1102 88-89 0.23551077776874507 1102 90-91 0.4048251833326262 1102 92-93 0.4423306432359482 1102 94-95 0.25553753665876755 1102 96-97 0.4643063117988966 1102 98-99 0.31427838513594963 1102 100-101 -0.12194796763778726 1103 1 0.1634023626838541 1103 2 0.2622150583506091 1103 3 0.2296415633722262 1103 4 0.2198667691193208 1103 5 0.12140373101509994 1103 6 0.17796049911919454 1103 7 0.2658850481759174 1103 8 0.29131653659230494 1103 9 0.37435136339733077 1103 10-11 0.40194682679965155 1103 12-13 0.39243621950183893 1103 14-15 0.45554964318390034 1103 16-17 0.567969982961749 1103 18-19 0.5522219549247112 1103 20-21 0.5135495230260076 1103 22-23 0.4682677954064971 1103 24-25 0.49887469457951283 1103 26-27 0.4337217819894903 1103 28-29 0.48814300882295214 1103 30-31 0.36477662632074725 1103 32-33 0.4485872561346156 1103 34-35 0.33860261130195823 1103 36-37 0.24307793355355756 1103 38-39 0.5603096303621129 1103 40-41 0.4643064085081292 1103 42-43 0.35294752880825087 1103 44-45 0.39469771033177636 1103 46-47 0.4142263000316575 1103 48-49 0.18984547417441178 1103 50-51 0.14635498671469094 1103 52-53 0.3107388409811449 1103 54-55 0.34658481431428356 1103 56-57 0.2832693087394489 1103 58-59 0.2559414514033804 1103 60-61 0.4373676910681539 1103 62-63 0.34635147805316535 1103 64-65 0.20764741091126382 1103 66-67 0.24090563673555465 1103 68-69 0.26561943739280736 1103 70-71 0.1310948174018307 1103 72-73 0.2614382704435734 1103 74-75 0.25297899449347483 1103 76-77 0.25757338828391596 1103 78-79 0.20718567231909546 1103 80-81 0.15907822282397888 1103 82-83 0.036721587798655975 1103 84-85 0.14066757551052333 1103 86-87 0.2504178095976357 1103 88-89 0.11176149348434095 1103 90-91 0.04680467871234484 1103 92-93 0.046012027296374214 1103 94-95 0.034724945723549894 1103 96-97 0.12377063458540505 1103 98-99 0.11175476399471407 1103 100-101 -0.04616209530787785 1104 1 0.35101529398129827 1104 2 0.36381332199907845 1104 3 0.3900956004406737 1104 4 0.38798039540044016 1104 5 0.3314345011547175 1104 6 0.35582974221465946 1104 7 0.3120280449728057 1104 8 0.39851670382008564 1104 9 0.37469757717055785 1104 10-11 0.49717251330549317 1104 12-13 0.46251795321771993 1104 14-15 0.43017223040904184 1104 16-17 0.5380561473050776 1104 18-19 0.5068981981843379 1104 20-21 0.4747686101690647 1104 22-23 0.6626747814835383 1104 24-25 0.5384555345015087 1104 26-27 0.5031014316631826 1104 28-29 0.537186930541715 1104 30-31 0.5953149532362971 1104 32-33 0.5506709007005952 1104 34-35 0.6011766468026423 1104 36-37 0.45565446838255497 1104 38-39 0.8918107383029863 1104 40-41 0.781910825048449 1104 42-43 0.7193783118989288 1104 44-45 0.6513361251214391 1104 46-47 0.6766070636105397 1104 48-49 0.6498576413590271 1104 50-51 0.43808780511066203 1104 52-53 0.48116008572616664 1104 54-55 0.7506248081114109 1104 56-57 0.7175422285740609 1104 58-59 0.4784028296806966 1104 60-61 0.5979356179832536 1104 62-63 0.4974162771156969 1104 64-65 0.47230199521900573 1104 66-67 0.34970267594036386 1104 68-69 0.33540054167558253 1104 70-71 0.40638333361309975 1104 72-73 0.40932622441957633 1104 74-75 0.6205239667821694 1104 76-77 0.41731398072697345 1104 78-79 0.21878397572959685 1104 80-81 0.23156584404850378 1104 82-83 0.09317360381935202 1104 84-85 0.08426341594659092 1104 86-87 0.008175951869624498 1104 88-89 0.016998040707733253 1104 90-91 0.2511641037077865 1104 92-93 0.23594805585233658 1104 94-95 0.1780835771822531 1104 96-97 0.09626652063421304 1104 98-99 -0.005186535303824513 1104 100-101 -0.12471513190656935 1105 1 0.22501981463500442 1105 2 0.3448430288447888 1105 3 0.3786740677627094 1105 4 0.3335234007533927 1105 5 0.26977177375099615 1105 6 0.2867451423312275 1105 7 0.2588506835119162 1105 8 0.24402089004865246 1105 9 0.28115746658444607 1105 10-11 0.38441747380891655 1105 12-13 0.4676421314028758 1105 14-15 0.47352598769165866 1105 16-17 0.4798995072992298 1105 18-19 0.3910561780414028 1105 20-21 0.551047920758613 1105 22-23 0.5879829150041189 1105 24-25 0.39877201252085115 1105 26-27 0.4424871432145068 1105 28-29 0.5073220124920041 1105 30-31 0.5844399529674718 1105 32-33 0.4944320974909786 1105 34-35 0.37650773844804064 1105 36-37 0.2735564828907968 1105 38-39 0.8638174182032827 1105 40-41 0.6843309985193997 1105 42-43 0.4415726354891305 1105 44-45 0.27617402032915805 1105 46-47 0.26878205291108515 1105 48-49 0.303027314267581 1105 50-51 0.2732011548032247 1105 52-53 0.3254198555642418 1105 54-55 0.4093420672446513 1105 56-57 0.31282059718154187 1105 58-59 0.38859193580852747 1105 60-61 0.5141736792206828 1105 62-63 0.3997375209621623 1105 64-65 0.44556357589934237 1105 66-67 0.3695638733759381 1105 68-69 0.3427560130214715 1105 70-71 0.3246739157279137 1105 72-73 0.2786477177407498 1105 74-75 0.3035851719600622 1105 76-77 0.3745011104129574 1105 78-79 0.3733779126340018 1105 80-81 0.23258874410592512 1105 82-83 0.17127370339416714 1105 84-85 0.3237182707028481 1105 86-87 0.34872828026436764 1105 88-89 0.14641223753897137 1105 90-91 0.26557771986514567 1105 92-93 0.257680313576909 1105 94-95 0.34865760557505254 1105 96-97 0.16130710328668485 1105 98-99 0.09293579117683848 1105 100-101 -0.1199087113739381 1106 1 0.20029923812219863 1106 2 0.2519258385687557 1106 3 0.192415173121816 1106 4 0.20523423536068464 1106 5 0.14787324597089224 1106 6 0.12835654238624983 1106 7 0.24633268260292596 1106 8 0.26599686330703776 1106 9 0.31298468607511154 1106 10-11 0.3935267651500354 1106 12-13 0.3304589549592052 1106 14-15 0.31298937165092156 1106 16-17 0.2477479943159011 1106 18-19 0.39990559621776356 1106 20-21 0.4036665806344004 1106 22-23 0.420165819355681 1106 24-25 0.3468426022855482 1106 26-27 0.3518545189666682 1106 28-29 0.4321600924891058 1106 30-31 0.3106536853558026 1106 32-33 0.3414142935968343 1106 34-35 0.37441325899552425 1106 36-37 0.4494175536639702 1106 38-39 0.9272612377316349 1106 40-41 0.6988215413028556 1106 42-43 0.5054848061694912 1106 44-45 0.3949288764176231 1106 46-47 0.2283602496659327 1106 48-49 0.2590560945592415 1106 50-51 0.23489386001358525 1106 52-53 0.3223032671156645 1106 54-55 0.28380518918928743 1106 56-57 0.4113189510242421 1106 58-59 0.43306564415197357 1106 60-61 0.4664372588847243 1106 62-63 0.44775686671162873 1106 64-65 0.37771791057868853 1106 66-67 0.39184191927082423 1106 68-69 0.3802627775338081 1106 70-71 0.36069437705506147 1106 72-73 0.3230720464038406 1106 74-75 0.42594978345787027 1106 76-77 0.5008954565403272 1106 78-79 0.6057180005701461 1106 80-81 0.5923677095713913 1106 82-83 0.6174815958794859 1106 84-85 0.6740545060161729 1106 86-87 0.8429450631076492 1106 88-89 0.7911623461238833 1106 90-91 0.727375734801992 1106 92-93 0.7069887965021238 1106 94-95 0.7131718556438322 1106 96-97 0.8152241968188569 1106 98-99 0.6998106771752823 1106 100-101 0.3799102717875904 1107 1 0.20534305226722438 1107 2 0.18998869083888437 1107 3 0.23472870121370448 1107 4 0.17699772749097065 1107 5 0.14049712727970842 1107 6 0.14365485439171266 1107 7 0.2092844940567531 1107 8 0.24355701736876512 1107 9 0.18521047946736502 1107 10-11 0.4195189982179741 1107 12-13 0.3864930311765207 1107 14-15 0.5041724430473948 1107 16-17 0.4700461945913119 1107 18-19 0.46759442364705706 1107 20-21 0.3537066776992859 1107 22-23 0.28627753587282 1107 24-25 0.3738595112516663 1107 26-27 0.3492258395530996 1107 28-29 0.27043288602597926 1107 30-31 0.2472316402894137 1107 32-33 0.3206062627690116 1107 34-35 0.3456679198561048 1107 36-37 0.2743714952441465 1107 38-39 0.7108877536449469 1107 40-41 0.6038050405625839 1107 42-43 0.4149880523872369 1107 44-45 0.4901198646869318 1107 46-47 0.5314444830066662 1107 48-49 0.5573875681433123 1107 50-51 0.4589348799397541 1107 52-53 0.4809309317687678 1107 54-55 0.5243613388650061 1107 56-57 0.40717538611947646 1107 58-59 0.4247366760298732 1107 60-61 0.46678575026288627 1107 62-63 0.5738801528523112 1107 64-65 0.47089739500492556 1107 66-67 0.5762012344381091 1107 68-69 0.6522404673140869 1107 70-71 0.5154773909903625 1107 72-73 0.5750419537743063 1107 74-75 0.5691826419626302 1107 76-77 0.6698426740023322 1107 78-79 0.7270064222887278 1107 80-81 0.6439758727340354 1107 82-83 0.7238192296109389 1107 84-85 0.7202079689467027 1107 86-87 0.9122634081677994 1107 88-89 0.7812262305669435 1107 90-91 0.8189260643573029 1107 92-93 0.747487557556024 1107 94-95 0.44563785415506985 1107 96-97 0.7211752977468926 1107 98-99 0.8011342430764259 1107 100-101 0.473405599197779 1108 1 0.13870103359636943 1108 2 0.20948441525188244 1108 3 0.253062048551584 1108 4 0.2214453462985162 1108 5 0.2920626951499301 1108 6 0.16380879232918488 1108 7 0.22580845442949737 1108 8 0.2286029662745861 1108 9 0.3289713154825691 1108 10-11 0.4418772512352547 1108 12-13 0.47386177359656045 1108 14-15 0.603452049351688 1108 16-17 0.5810434020854203 1108 18-19 0.5723386026587249 1108 20-21 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-1.1834538912693624 2316 78-79 -1.1549250360528944 2316 80-81 -0.9779627551065566 2316 82-83 -1.0470052783734154 2316 84-85 -1.0441251779328518 2316 86-87 -0.9540176346315334 2316 88-89 -1.0899981778713759 2316 90-91 -1.108876339882297 2316 92-93 -1.3111305078650695 2316 94-95 -1.1104381206900555 2316 96-97 -1.0760074558243993 2316 98-99 -1.1984433103847074 2316 100-101 -0.4486795391875944 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9052.0 3 2308.0 4 2320.0 5 2657.0 6 3054.0 7 3477.0 8 4267.0 9 5227.0 10 6164.0 11 7274.0 12 8858.0 13 11397.0 14 11667.0 15 12219.0 16 14774.0 17 18043.0 18 16414.0 19 12546.0 20 12271.0 21 13161.0 22 14294.0 23 15766.0 24 17916.0 25 20350.0 26 23435.0 27 27209.0 28 31974.0 29 37806.0 30 45586.0 31 55465.0 32 69034.0 33 89315.0 34 121889.0 35 177081.0 36 274019.0 37 422719.0 38 379339.0 39 52633.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.3326461300358 18.81746642800194 14.778860584363812 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29.001179507592166 21.77732964944527 20-21 23.215444124711357 26.131637595894514 28.78271148026119 21.870206799132937 22-23 22.858725194080247 25.96952293586935 28.78694355936587 22.38480831068454 24-25 22.65663110881396 26.207730971692573 28.854522724105863 22.281115195387606 26-27 22.342519372856458 26.536927917920234 28.73423839623173 22.38631431299158 28-29 22.244996247612672 26.55094609932262 29.066973038438306 22.137084614626406 30-31 22.402612451691063 26.258008391032455 28.860701932942973 22.478677224333506 32-33 22.213889042052415 26.308120881968172 28.833237124952 22.644752951027414 34-35 22.800185166265912 26.517515272061942 28.37885663252562 22.30344292914653 36-37 22.828350404215055 26.273758248125745 28.38546930202125 22.512422045637955 38-39 22.4673937664719 26.47965201752828 28.548378653539537 22.50457556246028 40-41 22.626346973797236 26.28924984297128 28.592949263579314 22.49145391965217 42-43 22.571720261195498 26.31942699631781 28.26310277178323 22.84574997070346 44-45 22.58338373251442 26.603770445920212 28.12347701503755 22.689368806527817 46-47 22.63683399650176 26.822661104908818 27.755990229979126 22.7845146686103 48-49 22.912206091422725 26.70012454945641 27.763370714483216 22.624298644637644 50-51 22.656072033716868 26.931532490411612 27.61744321900672 22.794952256864796 52-53 22.978562633481914 26.898795506198653 27.44789433811568 22.674747522203756 54-55 22.54288309024434 26.85553526551894 27.547125404169698 23.05445624006702 56-57 22.676787031871765 27.126767307822586 27.548590494744694 22.647855165560955 58-59 22.814746235457932 26.61467297183752 27.691863385356374 22.878717407348176 60-61 22.764047997794286 26.703026113686295 27.891702850125682 22.641223038393736 62-63 22.63028429468067 26.798701494133258 27.865194987534416 22.70581922365166 64-65 22.407754086258898 26.604988733594343 27.791136082863193 23.19612109728356 66-67 22.436591077602976 26.922700455394132 27.645409230724056 22.995299236278836 68-69 22.4148462852472 26.79056517887297 27.85663242081769 22.937956115062136 70-71 22.510316555456118 26.795171193764283 27.679227132583673 23.015285118195923 72-73 22.598028132343973 26.73495185827923 27.691228389569638 22.975791619807158 74-75 22.46639342396886 27.05423277801392 27.357917345429815 23.1214564525874 76-77 22.558412578261223 26.908330974477135 27.397806887599135 23.135449559662508 78-79 22.653388715208976 26.923492776383444 27.39930234492341 23.023816163484163 80-81 22.544292591740074 27.23928652412772 27.320473403408286 22.895947480723926 82-83 22.64108292502488 27.085724714477657 27.30208660542774 22.97110575506973 84-85 22.708359428821943 26.83848425659172 27.23635075468153 23.216805559904806 86-87 22.61832939199686 26.753087380236952 27.424753745982212 23.20382948178398 88-89 22.62353111866802 26.736117482614663 27.417511558621054 23.22283984009626 90-91 22.527218539464833 26.899045408728973 27.516058516158605 23.057677535647596 92-93 22.629145895890385 26.54185846750215 27.463456893450505 23.365538743156964 94-95 22.542943368005183 26.616420870457276 27.435863928158206 23.40477183337934 96-97 22.734269314273075 26.48149670811138 27.479145498932066 23.30508847868348 98-99 22.591745364279898 26.52934226805738 27.174046036146088 23.704866331516634 100-101 22.665656368195563 26.1486171233847 27.752853330645294 23.432873177774443 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2680.0 1 1573.5 2 515.0 3 526.0 4 469.0 5 477.0 6 702.5 7 1359.0 8 2145.5 9 2517.5 10 2312.0 11 2128.0 12 2242.5 13 2391.0 14 2619.5 15 2793.5 16 2915.0 17 3012.5 18 3185.0 19 3349.5 20 3542.0 21 3944.0 22 4562.5 23 5533.5 24 6688.5 25 8103.5 26 10126.0 27 12684.5 28 14938.0 29 17705.0 30 21268.5 31 24803.5 32 28742.0 33 32527.0 34 36474.5 35 40091.0 36 44091.0 37 49179.5 38 52814.0 39 54680.5 40 56939.5 41 61668.0 42 66820.0 43 70603.0 44 73711.0 45 76085.5 46 75326.5 47 75868.0 48 81925.5 49 83846.5 50 80177.5 51 77041.0 52 73979.0 53 72190.0 54 70828.0 55 65861.0 56 58541.5 57 52180.0 58 46907.5 59 41123.5 60 35183.5 61 29498.5 62 24717.5 63 20059.5 64 15793.5 65 12465.0 66 9541.5 67 7959.0 68 6355.0 69 4404.0 70 3249.0 71 2589.5 72 2192.0 73 1803.5 74 1290.5 75 985.5 76 723.0 77 462.5 78 299.5 79 209.0 80 148.5 81 99.0 82 68.0 83 49.5 84 33.5 85 25.5 86 19.5 87 10.5 88 5.5 89 7.5 90 10.5 91 8.0 92 5.0 93 4.0 94 2.5 95 2.0 96 2.5 97 3.0 98 1.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29415733106411496 2 0.18650909432761986 3 0.18699619042145016 4 0.20794132245615282 5 0.22070324011450654 6 0.11602628955037621 7 0.13473077955345952 8 0.14720043955551507 9 0.14749269721181324 10-11 0.134657715139385 12-13 0.1251349865050027 14-15 0.14040544904658248 16-17 0.11692741732396227 18-19 0.14491108791451268 20-21 0.17196927592678554 22-23 0.17403943432556432 24-25 0.19817504577485542 26-27 0.17825281553719635 28-29 0.17637749557594973 30-31 0.16629460643366262 32-33 0.17593910909150245 34-35 0.3533395064644958 36-37 0.4695606344524041 38-39 0.43702261538454046 40-41 0.4272319838985515 42-43 0.4494192109725214 44-45 0.452950657652791 46-47 0.4571153292550401 48-49 0.4684890230459775 50-51 0.46252209589655635 52-53 0.469317086405489 54-55 0.45236614234019473 56-57 0.41522506518563473 58-59 0.40487427319174096 60-61 0.3599153037312048 62-63 0.46554209167830424 64-65 0.4636424169123661 66-67 0.4723857917966198 68-69 0.48285835781397113 70-71 0.47669659222701793 72-73 0.4696093440617871 74-75 0.47465078863293075 76-77 0.45136759534784265 78-79 0.438045517181584 80-81 0.4351472954232938 82-83 0.4473977621831257 84-85 0.4484693735895524 86-87 0.45105098288685297 88-89 0.4568230715987419 90-91 0.47504046550799495 92-93 0.4796922332040742 94-95 0.4786693314070306 96-97 0.4763312701566452 98-99 0.4734086935936635 100-101 0.4560437178486134 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2052983.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.49380668266694 #Duplication Level Percentage of deduplicated Percentage of total 1 74.76298267683838 38.498305769806926 2 13.705349495944692 14.114812349251254 3 4.743478323404015 7.327792673663623 4 2.174394325838882 4.478713642665412 5 1.2038120898007467 3.0994433517228472 6 0.7374558930972883 2.278464671768716 7 0.483605987471311 1.743189926059956 8 0.32887520292936306 1.3548028897893987 9 0.24428881100145347 1.1321424727602665 >10 1.4261423107726945 13.600530538513066 >50 0.12143594745482134 4.302128946323118 >100 0.0650464531792097 6.085444981725594 >500 0.002183245216635564 0.737694382065197 >1k 7.593896405688891E-4 0.6512748440655086 >5k 1.8984741014222228E-4 0.5952585598191057 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6802 0.3313227630233665 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5376 0.26186286004316645 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3641 0.17735168776361032 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 4.8709609383029474E-5 14-15 0.0 0.0 0.0 2.4354804691514737E-5 4.8709609383029474E-5 16-17 0.0 0.0 0.0 4.8709609383029474E-5 4.8709609383029474E-5 18-19 0.0 0.0 0.0 1.4612882814908843E-4 4.8709609383029474E-5 20-21 0.0 0.0 0.0 1.7048363284060316E-4 4.8709609383029474E-5 22-23 0.0 0.0 0.0 2.4354804691514737E-4 4.8709609383029474E-5 24-25 0.0 0.0 0.0 4.1403167975575053E-4 4.8709609383029474E-5 26-27 0.0 0.0 0.0 5.845153125963537E-4 4.8709609383029474E-5 28-29 0.0 0.0 0.0 0.0018753199612466349 4.8709609383029474E-5 30-31 0.0 0.0 0.0 0.005820798321272022 4.8709609383029474E-5 32-33 0.0 0.0 0.0 0.013906593478854916 4.8709609383029474E-5 34-35 0.0 0.0 0.0 0.02744786488733711 4.8709609383029474E-5 36-37 0.0 0.0 0.0 0.050243962078594905 4.8709609383029474E-5 38-39 0.0 0.0 0.0 0.08132069286496771 4.8709609383029474E-5 40-41 0.0 0.0 0.0 0.11709790095680286 4.8709609383029474E-5 42-43 0.0 0.0 0.0 0.1536544627987665 4.8709609383029474E-5 44-45 0.0 0.0 0.0 0.19956326964227175 4.8709609383029474E-5 46-47 0.0 0.0 0.0 0.25755205961276834 4.8709609383029474E-5 48-49 0.0 0.0 0.0 0.32216535645935696 4.8709609383029474E-5 50-51 0.0 0.0 0.0 0.39313525733043087 4.8709609383029474E-5 52-53 0.0 0.0 0.0 0.4679532173427642 9.741921876605895E-5 54-55 0.0 0.0 0.0 0.5495174582546469 9.741921876605895E-5 56-57 0.0 0.0 0.0 0.6525869917091374 9.741921876605895E-5 58-59 0.0 0.0 0.0 0.7652523182120845 9.741921876605895E-5 60-61 0.0 0.0 0.0 0.8920190766314188 9.741921876605895E-5 62-63 0.0 0.0 0.0 1.022000669270033 9.741921876605895E-5 64-65 0.0 0.0 0.0 1.1594835417536338 9.741921876605895E-5 66-67 0.0 0.0 0.0 1.3190075124830551 9.741921876605895E-5 68-69 0.0 0.0 0.0 1.5036656416541199 9.741921876605895E-5 70-71 0.0 0.0 0.0 1.7070282608282679 9.741921876605895E-5 72-73 0.0 0.0 0.0 1.9224221535200243 9.741921876605895E-5 74-75 0.0 0.0 0.0 2.147241355627397 9.741921876605895E-5 76-77 0.0 0.0 0.0 2.3935414954726855 9.741921876605895E-5 78-79 0.0 0.0 0.0 2.6795156121604515 9.741921876605895E-5 80-81 0.0 0.0 0.0 2.9883101808441666 9.741921876605895E-5 82-83 0.0 0.0 0.0 3.3117176323427913 1.4612882814908843E-4 84-85 0.0 0.0 0.0 3.6424315252488695 1.4612882814908843E-4 86-87 0.0 0.0 0.0 4.000154896557838 1.4612882814908843E-4 88-89 0.0 0.0 0.0 4.389442094747009 1.7048363284060316E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5655 0.0 38.015766 1 GTATCAA 8645 0.0 32.937027 1 GCGTATC 70 0.008430432 27.118155 1 ATCAACG 10780 0.0 26.260546 3 TCAACGC 10955 0.0 25.840418 4 CAACGCA 11035 0.0 25.567142 5 TATCAAC 11110 0.0 25.565891 2 GTACATG 9710 0.0 25.36567 1 AACGCAG 11395 0.0 24.867851 6 TACATGG 9685 0.0 24.676245 2 ACATGGG 10030 0.0 23.212864 3 GTGTACG 250 3.8744474E-10 22.779251 1 ACGCAGA 13030 0.0 21.78381 7 CGCAGAG 13090 0.0 21.756893 8 GAGTACT 6130 0.0 21.332243 12-13 CATGGGG 5355 0.0 20.011818 4 TGTACGC 120 0.0048122895 19.757727 2 GCAGAGT 14055 0.0 19.75639 9 GTACACG 265 1.8762876E-8 19.699036 1 ATTCGCG 290 0.0 19.667074 80-81 >>END_MODULE