FastQCFastQC Report
Fri 27 May 2016
SRR939249_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939249_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2052983
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69920.3405775888061421No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60070.2925986235638581No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42060.20487261706502194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58350.039.3176231
GTATCAA88350.033.3476451
ATCAACG111700.026.3825363
TATCAAC113900.025.831292
TCAACGC115150.025.5920934
CAACGCA115500.025.4734545
AACGCAG118200.024.8915716
GTACATG96650.024.1802481
TACATGG96850.023.5680452
GAGTACT68450.022.25462512-13
ACATGGG98950.022.0127853
ACGCAGA136650.021.5655277
CGCAGAG137600.021.4511248
GTACTTT74350.020.58436414-15
AGTACTT72350.019.90713912-13
GCAGAGT147750.019.6246729
CATGGGG52050.019.3282814
AGAGTAC125450.018.70614410-11
TACACTG15750.018.6805325
ATGGGGA29350.018.2704165
Produced by FastQC (version 0.11.5)