##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939249_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2052983 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.33899501359729 33.0 31.0 34.0 31.0 34.0 2 32.54594801807906 34.0 31.0 34.0 31.0 34.0 3 32.634868871296064 34.0 31.0 34.0 31.0 34.0 4 36.15042988665761 37.0 35.0 37.0 35.0 37.0 5 36.092878508979375 37.0 35.0 37.0 35.0 37.0 6 36.123451582404726 37.0 36.0 37.0 35.0 37.0 7 36.06767372160412 37.0 35.0 37.0 35.0 37.0 8 36.04204808320381 37.0 35.0 37.0 35.0 37.0 9 37.751235641016024 39.0 38.0 39.0 35.0 39.0 10-11 37.67423816953185 39.0 37.0 39.0 35.0 39.0 12-13 37.600650370704486 39.0 37.0 39.0 35.0 39.0 14-15 38.900115587903066 40.0 38.0 41.0 35.0 41.0 16-17 38.90395853253534 40.0 38.0 41.0 35.0 41.0 18-19 39.008011513003275 40.0 38.5 41.0 35.0 41.0 20-21 38.988868392967696 40.0 38.5 41.0 35.0 41.0 22-23 38.91273868317468 40.0 38.0 41.0 35.0 41.0 24-25 38.84700920562908 40.0 38.0 41.0 35.0 41.0 26-27 38.69563264771311 40.0 38.0 41.0 34.0 41.0 28-29 38.53092548744924 40.0 38.0 41.0 34.0 41.0 30-31 38.33604394191282 40.0 38.0 41.0 34.0 41.0 32-33 38.28155201479993 40.0 38.0 41.0 34.0 41.0 34-35 38.189519104639444 40.0 38.0 41.0 33.5 41.0 36-37 38.040174224530844 40.0 38.0 41.0 33.0 41.0 38-39 37.90905915928188 40.0 37.0 41.0 33.0 41.0 40-41 37.722936819252766 40.0 37.0 41.0 33.0 41.0 42-43 37.533663454592656 40.0 37.0 41.0 33.0 41.0 44-45 37.28289980969156 40.0 37.0 41.0 32.0 41.0 46-47 36.969060143216 40.0 36.0 41.0 31.0 41.0 48-49 36.649507570203944 39.5 36.0 41.0 30.5 41.0 50-51 36.39875123174425 39.0 35.5 41.0 30.0 41.0 52-53 36.14264657817429 39.0 35.0 41.0 30.0 41.0 54-55 35.88952952849585 39.0 35.0 40.0 29.0 41.0 56-57 35.5796445952061 38.0 35.0 40.0 28.0 41.0 58-59 35.25611196001136 38.0 34.5 40.0 28.0 41.0 60-61 34.91395593631316 38.0 34.0 40.0 27.0 41.0 62-63 34.559399663806275 37.0 34.0 40.0 26.0 41.0 64-65 34.24428258782464 37.0 34.0 39.5 26.0 41.0 66-67 33.82823043347169 36.0 33.0 39.0 26.0 40.5 68-69 33.36047911745982 36.0 33.0 39.0 25.0 40.0 70-71 32.88281758787092 35.0 32.0 38.0 24.0 40.0 72-73 32.394880035538534 35.0 32.0 37.5 22.5 39.5 74-75 31.869821376991432 35.0 31.0 37.0 21.5 39.0 76-77 29.929438772751652 33.0 29.0 35.0 18.0 37.5 78-79 30.886967159494258 34.0 30.5 36.0 19.5 38.0 80-81 30.890642299522206 34.0 31.0 35.5 19.5 37.0 82-83 30.5365297715568 34.0 31.0 35.0 18.0 37.0 84-85 30.08683072387838 34.0 30.0 35.0 13.0 36.5 86-87 29.472822473444737 34.0 29.5 35.0 7.0 36.0 88-89 28.886079426863255 34.0 29.0 35.0 2.0 35.5 90-91 28.40290518723243 33.5 29.0 35.0 2.0 35.0 92-93 28.029093275492297 33.0 27.5 35.0 2.0 35.0 94-95 27.49725472641517 33.0 27.0 35.0 2.0 35.0 96-97 26.749287987284845 33.0 25.5 34.5 2.0 35.0 98-99 25.72687596536357 32.0 24.0 34.0 2.0 35.0 100-101 21.34518527430573 27.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22946960761324675 1101 2 0.24976163917912686 1101 3 0.24367225138165338 1101 4 0.13795440672645753 1101 5 0.24428925016198377 1101 6 0.18581120663769468 1101 7 0.2105659307935639 1101 8 0.21079805743312363 1101 9 0.21842391236796033 1101 10-11 0.3651187323701848 1101 12-13 0.350900781990525 1101 14-15 0.3830303694483703 1101 16-17 0.29658158058388295 1101 18-19 0.3793967356647485 1101 20-21 0.32549869044124335 1101 22-23 0.3089584824905742 1101 24-25 0.32325304016941203 1101 26-27 0.2711307160776357 1101 28-29 0.2914965986671163 1101 30-31 0.32560061730512047 1101 32-33 0.43338802962843204 1101 34-35 0.42573333988347173 1101 36-37 0.4513349883621913 1101 38-39 0.440824949931347 1101 40-41 0.4700467968576234 1101 42-43 0.37847264196485497 1101 44-45 0.6029995531537438 1101 46-47 0.4960251776807425 1101 48-49 0.45503845500621054 1101 50-51 0.5121276233517023 1101 52-53 0.454533785535574 1101 54-55 0.39011200229673904 1101 56-57 0.453909031537421 1101 58-59 0.5676318730770689 1101 60-61 0.5114164204994154 1101 62-63 0.4758051456064507 1101 64-65 0.37593542166171545 1101 66-67 0.3187291838283386 1101 68-69 0.36121413624469056 1101 70-71 0.36771895021140466 1101 72-73 0.3331723617185176 1101 74-75 0.37889096062413685 1101 76-77 0.4072251068987818 1101 78-79 0.585838593531971 1101 80-81 0.5964019732672448 1101 82-83 0.5947648796544094 1101 84-85 0.43884018278349757 1101 86-87 0.3829871745233149 1101 88-89 0.4284524985420788 1101 90-91 0.6143412326418449 1101 92-93 0.6013176016423039 1101 94-95 0.30834443393432664 1101 96-97 0.2605130963627893 1101 98-99 0.1047404532987315 1101 100-101 -0.15427889600377753 1102 1 0.23076799967115136 1102 2 0.27723975475792884 1102 3 0.3220981297421517 1102 4 0.19254712270088703 1102 5 0.1947234792820538 1102 6 0.20496623707092 1102 7 0.2281259338608166 1102 8 0.26540489889841723 1102 9 0.24902750900620418 1102 10-11 0.291013812836475 1102 12-13 0.34179474106581154 1102 14-15 0.3834067128834491 1102 16-17 0.3667858467127445 1102 18-19 0.2903481999015938 1102 20-21 0.41764647420938417 1102 22-23 0.4556357057995868 1102 24-25 0.4500977860345401 1102 26-27 0.46840342972279814 1102 28-29 0.4770354976744713 1102 30-31 0.4610407243590444 1102 32-33 0.6785630213981761 1102 34-35 0.5866897048008255 1102 36-37 0.6289947462224532 1102 38-39 0.67545872207738 1102 40-41 0.6081505679024488 1102 42-43 0.602070802307054 1102 44-45 0.7173788416095235 1102 46-47 0.6761178351352868 1102 48-49 0.7348978688963612 1102 50-51 0.806584867220522 1102 52-53 0.873424352186845 1102 54-55 0.8097063931507975 1102 56-57 0.8132521500883456 1102 58-59 0.8403991374537867 1102 60-61 0.8673320895893184 1102 62-63 0.9489446599086548 1102 64-65 0.9711358969700683 1102 66-67 0.9678567473887227 1102 68-69 0.8825571763847222 1102 70-71 0.6555607078145229 1102 72-73 0.747434956323275 1102 74-75 0.7174567793577644 1102 76-77 0.7634500954110095 1102 78-79 0.8094066291028419 1102 80-81 0.9479844318291093 1102 82-83 0.9336587557064 1102 84-85 0.7740377926351378 1102 86-87 0.7188104859650188 1102 88-89 1.0114768007284134 1102 90-91 0.9970421475700668 1102 92-93 0.8927073077917349 1102 94-95 0.6925005626965373 1102 96-97 0.37591489739170214 1102 98-99 0.24385261142581882 1102 100-101 0.20793948062562606 1103 1 0.07118197274955662 1103 2 0.10744039766760238 1103 3 0.17249299685942532 1103 4 0.11475984535954353 1103 5 0.1340273316293903 1103 6 0.1309120860936872 1103 7 0.16153803561182656 1103 8 0.13054112523865768 1103 9 0.16353230310966183 1103 10-11 0.09571173682090972 1103 12-13 0.13513621110739393 1103 14-15 0.25979024924468774 1103 16-17 0.18933486547135203 1103 18-19 0.1796615373754733 1103 20-21 0.23000973752024834 1103 22-23 0.23188532430115316 1103 24-25 0.21220662562387105 1103 26-27 0.15867391319284252 1103 28-29 0.26380595501348836 1103 30-31 0.286076809707275 1103 32-33 0.46712496938041426 1103 34-35 0.42810820619703094 1103 36-37 0.4586290245746767 1103 38-39 0.4621438565886038 1103 40-41 0.5276788698164765 1103 42-43 0.46363126587420567 1103 44-45 0.4952718268603675 1103 46-47 0.6576508568020856 1103 48-49 0.7021421321272427 1103 50-51 0.5090498740333516 1103 52-53 0.4350720442693188 1103 54-55 0.5231872844466281 1103 56-57 0.6855557827041281 1103 58-59 0.48533158227474615 1103 60-61 0.5072675907288371 1103 62-63 0.5579047984117125 1103 64-65 0.6276430835775884 1103 66-67 0.5013682625055864 1103 68-69 0.46299667990114557 1103 70-71 0.42164709263001043 1103 72-73 0.4516158037594167 1103 74-75 0.26014735605057027 1103 76-77 0.3956912388004241 1103 78-79 0.42713942688023465 1103 80-81 0.5993635865527445 1103 82-83 0.5697095417014459 1103 84-85 0.45771084364374914 1103 86-87 0.4981477513669432 1103 88-89 0.5645348950351163 1103 90-91 0.6523103485461803 1103 92-93 0.4557678073070832 1103 94-95 0.31826987575682253 1103 96-97 0.32292263592675496 1103 98-99 -0.09751767030120817 1103 100-101 0.1055712885138469 1104 1 0.09346376860783323 1104 2 0.04708758339553043 1104 3 0.08286821988187398 1104 4 0.037426083353537365 1104 5 0.11814722440820447 1104 6 0.05325909169957299 1104 7 0.09961405268223444 1104 8 0.05263620991192397 1104 9 0.17326235861179384 1104 10-11 0.1376430814084202 1104 12-13 0.07351654980045907 1104 14-15 0.14012439807855515 1104 16-17 0.1597270555248187 1104 18-19 0.08598728051381954 1104 20-21 0.19745343020965578 1104 22-23 0.07918516928599928 1104 24-25 0.1160071951299031 1104 26-27 0.049963924456861264 1104 28-29 0.05870198579612662 1104 30-31 0.03700369128967651 1104 32-33 0.012771508691159283 1104 34-35 0.10053516190722434 1104 36-37 0.18067190736811511 1104 38-39 0.2425287304264856 1104 40-41 0.11376084892096117 1104 42-43 0.030810512588011818 1104 44-45 0.09552102125301332 1104 46-47 0.18789627948110876 1104 48-49 0.052239520726779176 1104 50-51 0.09996450322675798 1104 52-53 0.19824023827172255 1104 54-55 0.31568708421634994 1104 56-57 0.10602062368720055 1104 58-59 0.002580781779649044 1104 60-61 -0.031736415580830624 1104 62-63 0.12189030117148292 1104 64-65 0.1438718186959349 1104 66-67 0.06286267338266072 1104 68-69 0.10297056760065004 1104 70-71 0.047483586572255376 1104 72-73 0.13157724025972328 1104 74-75 5.593078385288663E-4 1104 76-77 0.2222773324241274 1104 78-79 0.32909730979011087 1104 80-81 0.2732445549061353 1104 82-83 0.0868437153013808 1104 84-85 0.10654911040501247 1104 86-87 0.10946158577394982 1104 88-89 0.5024106818265075 1104 90-91 0.5858817523677651 1104 92-93 0.48673931326031195 1104 94-95 0.2024055597462926 1104 96-97 0.08511381977336896 1104 98-99 0.08325704194941963 1104 100-101 0.013097419275293731 1105 1 0.07301759600144209 1105 2 0.0046274186992150135 1105 3 0.06442375918811649 1105 4 0.03766695548269183 1105 5 0.01985687886423193 1105 6 0.1150137591347331 1105 7 0.038395433266650514 1105 8 0.07012578466508046 1105 9 0.0873032681621595 1105 10-11 0.08260272457356876 1105 12-13 0.1373648691796987 1105 14-15 0.082885276573613 1105 16-17 0.011518380687142837 1105 18-19 -0.03177284525584412 1105 20-21 0.07675875068787974 1105 22-23 0.04520391860048534 1105 24-25 0.12304921011129011 1105 26-27 0.07812902724688797 1105 28-29 0.1416568953938011 1105 30-31 0.1403367593769147 1105 32-33 0.25422458056353037 1105 34-35 0.3373393479207252 1105 36-37 0.13945777204528298 1105 38-39 0.24887850686703672 1105 40-41 0.3678610517266847 1105 42-43 0.28807753869897823 1105 44-45 0.16233696053603097 1105 46-47 0.2614464228215283 1105 48-49 0.3608760231774468 1105 50-51 0.47141083283015917 1105 52-53 0.44134581123554995 1105 54-55 0.3617737723751233 1105 56-57 0.370678606770241 1105 58-59 0.4435851217792717 1105 60-61 0.28775600017306857 1105 62-63 0.46297894641629966 1105 64-65 0.5353374180696662 1105 66-67 0.4104371233016906 1105 68-69 0.4366889558219995 1105 70-71 0.4753655033122257 1105 72-73 0.5453229739196601 1105 74-75 0.5124491432497429 1105 76-77 0.5823827784999551 1105 78-79 0.42557358711135507 1105 80-81 0.518147528601606 1105 82-83 0.5800742944483055 1105 84-85 0.5683009820029632 1105 86-87 0.6249222967338426 1105 88-89 0.580069630548703 1105 90-91 0.6138952347550699 1105 92-93 0.6410937221880637 1105 94-95 0.7267011163007062 1105 96-97 0.8559014071163986 1105 98-99 0.8567399238984166 1105 100-101 0.6099315099937606 1106 1 0.05658776704660795 1106 2 0.07978883482782351 1106 3 0.1140722564838228 1106 4 0.10247224334104033 1106 5 0.012115102344573359 1106 6 0.13190009819577142 1106 7 0.15839939371856815 1106 8 0.10118635942440335 1106 9 0.1913608670399256 1106 10-11 0.10750519970485328 1106 12-13 0.07437226907750727 1106 14-15 0.08984844134756287 1106 16-17 0.13636243159456995 1106 18-19 0.10146990827606572 1106 20-21 0.16703261644165224 1106 22-23 0.16659680853116043 1106 24-25 0.19265931722488716 1106 26-27 0.18851390154536318 1106 28-29 0.054127003051583245 1106 30-31 0.13072787667783814 1106 32-33 0.11720818650964304 1106 34-35 0.2107464753233259 1106 36-37 0.15437333279187015 1106 38-39 0.14295858861560617 1106 40-41 0.2150664641427582 1106 42-43 0.20310216303550988 1106 44-45 0.22402364444862854 1106 46-47 0.24145155730053602 1106 48-49 0.25865844723602294 1106 50-51 0.3882985323517829 1106 52-53 0.5156456023292719 1106 54-55 0.374274137683976 1106 56-57 0.5400250868556142 1106 58-59 0.5895520867731037 1106 60-61 0.6852272818579408 1106 62-63 0.5754921857132587 1106 64-65 0.6089879671414806 1106 66-67 0.7205696069686098 1106 68-69 0.6378946514154151 1106 70-71 0.5859880803067341 1106 72-73 0.5509472187380737 1106 74-75 0.6035080451464303 1106 76-77 0.5808507487229981 1106 78-79 0.6138706532529135 1106 80-81 0.7159967915846828 1106 82-83 0.9382222188389449 1106 84-85 0.8446939172823242 1106 86-87 1.000292392399416 1106 88-89 1.1576507769836404 1106 90-91 1.1531917607433932 1106 92-93 1.0150160610515861 1106 94-95 1.3717170185732783 1106 96-97 1.3594617828453792 1106 98-99 1.3943921821118224 1106 100-101 1.187502830880053 1107 1 0.1542391141193562 1107 2 0.12233162719903135 1107 3 0.1286984952485497 1107 4 0.051417893680124394 1107 5 0.12564324260013393 1107 6 0.17124189689496205 1107 7 0.044108732078299795 1107 8 0.08215113640201821 1107 9 0.18991369673670988 1107 10-11 0.2033036899463596 1107 12-13 0.16762708665037707 1107 14-15 0.17237910786332122 1107 16-17 0.15017923202291428 1107 18-19 0.20607040559574585 1107 20-21 0.23142395605605515 1107 22-23 0.18959324913067377 1107 24-25 0.15945251455710263 1107 26-27 0.12963905227211825 1107 28-29 0.21072937898406252 1107 30-31 0.16982602162025984 1107 32-33 0.17191480320250463 1107 34-35 0.2378321877572418 1107 36-37 0.15946974712756656 1107 38-39 0.21823454009864207 1107 40-41 0.2532178056531862 1107 42-43 0.32087761753742683 1107 44-45 0.2524643861621101 1107 46-47 0.2823508053233752 1107 48-49 0.31015488033850147 1107 50-51 0.22038155310696794 1107 52-53 0.17977089380687516 1107 54-55 0.2259382142446995 1107 56-57 0.5083793611108263 1107 58-59 0.40728783115407197 1107 60-61 0.3511251393010255 1107 62-63 0.3093537216968656 1107 64-65 0.2537268839790059 1107 66-67 0.46058439790037653 1107 68-69 0.43830280471099314 1107 70-71 0.40259273920840144 1107 72-73 0.4213460321745046 1107 74-75 0.37676688209937836 1107 76-77 0.3627853522965232 1107 78-79 0.48776139382974293 1107 80-81 0.4381195369712252 1107 82-83 0.659578936161406 1107 84-85 0.664186749871515 1107 86-87 0.7302719133918529 1107 88-89 0.8972045000372297 1107 90-91 0.9876608954351909 1107 92-93 0.8865376164888019 1107 94-95 1.193802738262388 1107 96-97 1.0358377191892316 1107 98-99 1.154122129105339 1107 100-101 1.2367581331001922 1108 1 0.1058716237054611 1108 2 0.07663900451223071 1108 3 0.08747332180227829 1108 4 0.08630461093119379 1108 5 0.09523517822405125 1108 6 0.019197373651621774 1108 7 0.10202451995989037 1108 8 0.08613262799789112 1108 9 0.14505434436280495 1108 10-11 0.0032123803255217354 1108 12-13 0.06590718871549228 1108 14-15 0.13071979358176833 1108 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-0.2972085221287699 2316 98-99 -0.19818365665692284 2316 100-101 0.3183732051189416 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 10.0 4 13.0 5 39.0 6 106.0 7 256.0 8 519.0 9 1039.0 10 1705.0 11 2673.0 12 3999.0 13 5567.0 14 7053.0 15 9419.0 16 12343.0 17 17365.0 18 16430.0 19 11711.0 20 10871.0 21 11806.0 22 12374.0 23 13834.0 24 15849.0 25 18957.0 26 22495.0 27 27221.0 28 33048.0 29 40359.0 30 49426.0 31 59920.0 32 74612.0 33 96377.0 34 133016.0 35 191791.0 36 300775.0 37 471929.0 38 355344.0 39 22728.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.52991483054532 19.436945585150404 15.265772275031777 22.767367309272498 2 15.736808341812864 20.537432604166717 39.74631061241131 23.97944844160911 3 19.203714789649986 24.388706579645326 29.96434943689256 26.443229193812122 4 13.139952936775416 17.063024876484608 36.586518251734184 33.21050393500579 5 14.074836469663898 37.47181540227075 33.63198818499715 14.821359943068208 6 32.2374807779704 35.916761122717524 17.321088386995896 14.524669712316177 7 29.124790609566663 32.70860011992306 20.090083551592976 18.076525718917303 8 26.527594237263536 35.40910957372759 19.313506249199335 18.749789939809535 9 26.48044333538076 17.112562549227146 20.053794892602617 36.35319922278947 10-11 25.31891399003304 26.701560607175022 26.800976919925784 21.17854848286615 12-13 26.024034295461774 23.935634147969076 27.598962095643266 22.44136946092588 14-15 22.790637182922822 25.380202786185052 26.012463525058205 25.81669650583392 16-17 22.45142205402093 28.76578175890609 26.400079689677874 22.38271649739511 18-19 22.312105025762524 26.916894546566894 29.055076956349353 21.71592347132123 20-21 23.073474061889456 26.148487347435417 28.925202985119704 21.85283560555543 22-23 22.756861600899764 26.019479946984458 28.938646837309417 22.285011614806358 24-25 22.600406335561473 26.265707022415675 28.969017278759736 22.164869363263115 26-27 22.331448188890157 26.61675226980857 28.83399095413183 22.21780858716944 28-29 22.189857392876608 26.595105755868413 29.145687031991983 22.069349819262996 30-31 22.33535299610372 26.27661797491747 28.97408307813557 22.413945950843235 32-33 22.1809922439689 26.413979073377618 28.905037206835125 22.499991475818355 34-35 22.810102535375922 26.508536496261474 28.484863245573443 22.196497722789157 36-37 22.794743041530147 26.242777016224494 28.59636636785849 22.366113574386866 38-39 22.433355708032582 26.542076287657444 28.70083695663822 22.32373104767175 40-41 22.57356943749292 26.24229294305759 28.749103405779366 22.435034213670125 42-43 22.565217518373327 26.31657652110887 28.40527604803953 22.712929912478273 44-45 22.598726952345345 26.598999730874755 28.213751204061477 22.58852211271842 46-47 22.625565732909525 26.89670154850623 27.829200354509315 22.648532364074928 48-49 22.937991421225988 26.74186326763028 27.776289422077433 22.5438558890663 50-51 22.664270153301356 26.999036767239858 27.63053265188573 22.706160427573057 52-53 22.951619388679344 26.951192692189384 27.45684751664835 22.640340402482924 54-55 22.568672025048432 26.903047906387922 27.51376898883235 23.0145110797313 56-57 22.65885161509361 27.154503384500877 27.49333097576355 22.69331402464196 58-59 22.839912398206266 26.667400578491385 27.688918158764615 22.803768864537737 60-61 22.792606202544224 26.819978333765228 27.80260637790044 22.584809085790106 62-63 22.590413163882907 26.885280478024193 27.81762151595959 22.70668484213331 64-65 22.411253483956507 26.606394990311657 27.819143080650488 23.16320844508135 66-67 22.3835140549827 26.959584953138695 27.599802822540774 23.057098169337834 68-69 22.431442291111974 26.779300145839485 27.809163629561866 22.980093933486675 70-71 22.47514957503301 26.80287659469173 27.66255249069281 23.05942133958245 72-73 22.53927577578577 26.810572712974245 27.595869035447446 23.054282475792544 74-75 22.457492341631667 27.068100417782322 27.263523370627034 23.21088386995898 76-77 22.44701122919493 26.875227694708887 27.43368406864684 23.24407700744934 78-79 22.53480855507812 26.922017487266675 27.40468182950682 23.13849212814838 80-81 22.40538144550848 27.28353417958285 27.269578856101862 23.041505518806808 82-83 22.594171505560446 27.039118200199418 27.236148570153773 23.130561724086366 84-85 22.482139403979478 26.819583990710104 27.245208557499012 23.453068047811403 86-87 22.391613080181635 26.69376161887057 27.44631700850273 23.468308292445066 88-89 22.353515510410023 26.704634348586247 27.41503107557878 23.52681906542495 90-91 22.339064404521263 26.77160239206955 27.453215708024302 23.436117495384885 92-93 22.31012141844331 26.49829540721964 27.43644248393679 23.75514069040026 94-95 22.164114364317676 26.51536812530839 27.44148392850793 23.879033581865997 96-97 22.333502030947162 26.360568986689124 27.468055020426373 23.837873961937337 98-99 22.1054136452443 26.41064521361397 27.197574841242893 24.28636629989883 100-101 22.092562212125227 25.860312054305556 27.975460989315025 24.071664744254196 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 493.0 1 344.0 2 259.0 3 334.0 4 299.0 5 300.5 6 537.5 7 1252.5 8 2046.0 9 2311.5 10 2063.0 11 1823.5 12 1876.5 13 2066.0 14 2284.5 15 2436.0 16 2581.5 17 2690.0 18 2850.0 19 2997.5 20 3285.0 21 3834.5 22 4480.5 23 5455.0 24 6644.5 25 8003.5 26 10083.5 27 12719.0 28 14943.5 29 17794.0 30 21272.5 31 24996.0 32 29085.5 33 32427.5 34 36514.5 35 40473.0 36 44299.0 37 49283.0 38 52954.5 39 54798.0 40 57069.0 41 62032.0 42 67576.5 43 71014.5 44 73852.5 45 76691.0 46 76303.0 47 76808.5 48 82976.0 49 84876.5 50 80900.0 51 77659.0 52 74140.0 53 72672.5 54 71756.0 55 66294.0 56 58645.0 57 52360.0 58 47135.5 59 41086.5 60 34952.0 61 29261.5 62 24369.0 63 19680.5 64 15583.5 65 12150.0 66 9029.0 67 7607.5 68 6035.5 69 4042.0 70 2995.0 71 2430.5 72 2045.5 73 1720.5 74 1367.0 75 1105.5 76 741.0 77 400.5 78 263.0 79 175.0 80 102.0 81 57.0 82 35.5 83 19.5 84 9.5 85 4.0 86 1.0 87 1.5 88 2.0 89 2.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29250120434509197 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001583062304948458 16-17 9.498373829690747E-4 18-19 4.870960938302947E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 7.306441407454421E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0016074171096399725 36-37 0.007842247110667744 38-39 0.003360963047429034 40-41 0.0036775755084187253 42-43 3.4096726568120633E-4 44-45 0.0017291911330975464 46-47 2.1919324222363263E-4 48-49 4.3838648444726526E-4 50-51 2.4354804691514734E-5 52-53 7.306441407454421E-5 54-55 0.0 56-57 0.0010472566017351336 58-59 0.00277644773483268 60-61 9.2548257827756E-4 62-63 0.001583062304948458 64-65 4.870960938302947E-5 66-67 4.8709609383029473E-4 68-69 0.0019970939847042085 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.2574302855893108 78-79 1.7048363284060316E-4 80-81 1.4612882814908843E-4 82-83 0.0 84-85 0.0 86-87 0.10572420716586549 88-89 0.3724823829520264 90-91 0.2744542940686796 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.9483843753211787E-4 100-101 3.4096726568120633E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2052983.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.70610794401476 #Duplication Level Percentage of deduplicated Percentage of total 1 73.03832721744013 36.3045097672035 2 14.581206408941533 14.495500394336153 3 5.136269493284124 7.65911897588192 4 2.3491398918224635 4.670664041540741 5 1.291281990448706 3.2092301001702808 6 0.7574239170692372 2.258915698873319 7 0.4886157011928443 1.7001029350642385 8 0.37159241201619 1.4776330034282839 9 0.2627189202820884 1.1752861509428845 >10 1.5208621261314172 14.064902046995687 >50 0.12777878450650534 4.3759655036932035 >100 0.07193417502293419 6.6163421648090655 >500 0.0016700810797278 0.5550166923538433 >1k 9.824006351339982E-4 0.8020542998338783 >5k 1.9648012702679966E-4 0.634758224873095 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6992 0.3405775888061421 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6007 0.2925986235638581 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4206 0.20487261706502194 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.4354804691514737E-5 2.4354804691514737E-5 16-17 0.0 0.0 0.0 4.8709609383029474E-5 4.8709609383029474E-5 18-19 0.0 0.0 0.0 9.741921876605895E-5 4.8709609383029474E-5 20-21 0.0 0.0 0.0 2.1919324222363263E-4 4.8709609383029474E-5 22-23 0.0 0.0 0.0 2.9225765629817686E-4 4.8709609383029474E-5 24-25 0.0 0.0 0.0 4.6274128913878E-4 4.8709609383029474E-5 26-27 0.0 0.0 0.0 6.575797266708979E-4 4.8709609383029474E-5 28-29 0.0 0.0 0.0 0.0019483843753211791 4.8709609383029474E-5 30-31 0.0 0.0 0.0 0.005942572344729596 4.8709609383029474E-5 32-33 0.0 0.0 0.0 0.014052722307004004 4.8709609383029474E-5 34-35 0.0 0.0 0.0 0.027569638910794683 4.8709609383029474E-5 36-37 0.0 0.0 0.0 0.05065799375835066 4.8709609383029474E-5 38-39 0.0 0.0 0.0 0.08207569181040467 4.8709609383029474E-5 40-41 0.0 0.0 0.0 0.1183156411913786 4.8709609383029474E-5 42-43 0.0 0.0 0.0 0.1551644606896404 4.8709609383029474E-5 44-45 0.0 0.0 0.0 0.20158471843166748 7.306441407454421E-5 46-47 0.0 0.0 0.0 0.26003624969130285 9.741921876605895E-5 48-49 0.0 0.0 0.0 0.3250635782176472 9.741921876605895E-5 50-51 0.0 0.0 0.0 0.3964718655731684 9.741921876605895E-5 52-53 0.0 0.0 0.0 0.47192305050748107 9.741921876605895E-5 54-55 0.0 0.0 0.0 0.5543153547788754 9.741921876605895E-5 56-57 0.0 0.0 0.0 0.6577015006943554 9.741921876605895E-5 58-59 0.0 0.0 0.0 0.7712192453615057 9.741921876605895E-5 60-61 0.0 0.0 0.0 0.8993498728435647 9.741921876605895E-5 62-63 0.0 0.0 0.0 1.029769851966626 9.741921876605895E-5 64-65 0.0 0.0 0.0 1.168494819489494 1.4612882814908843E-4 66-67 0.0 0.0 0.0 1.3291147564300339 1.4612882814908843E-4 68-69 0.0 0.0 0.0 1.5145765941559186 1.4612882814908843E-4 70-71 0.0 0.0 0.0 1.7188646959083442 1.4612882814908843E-4 72-73 0.0 0.0 0.0 1.9347700394986223 1.4612882814908843E-4 74-75 0.0 0.0 0.0 2.1597353704341438 1.4612882814908843E-4 76-77 0.0 0.0 0.0 2.40632776793573 1.4612882814908843E-4 78-79 0.0 0.0 0.0 2.693933656537828 1.4612882814908843E-4 80-81 0.0 0.0 0.0 3.0034101597529057 1.4612882814908843E-4 82-83 0.0 0.0 0.0 3.32745083617351 1.948384375321179E-4 84-85 0.0 0.0 0.0 3.6583839223218115 1.948384375321179E-4 86-87 0.0 0.0 0.0 4.017446807888813 2.679028516066621E-4 88-89 0.0 0.0 0.0 4.408731100062689 3.4096726568120633E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5835 0.0 39.317623 1 GTATCAA 8835 0.0 33.347645 1 ATCAACG 11170 0.0 26.382536 3 TATCAAC 11390 0.0 25.83129 2 TCAACGC 11515 0.0 25.592093 4 CAACGCA 11550 0.0 25.473454 5 AACGCAG 11820 0.0 24.891571 6 GTACATG 9665 0.0 24.180248 1 TACATGG 9685 0.0 23.568045 2 GAGTACT 6845 0.0 22.254625 12-13 ACATGGG 9895 0.0 22.012785 3 ACGCAGA 13665 0.0 21.565527 7 CGCAGAG 13760 0.0 21.451124 8 GTACTTT 7435 0.0 20.584364 14-15 AGTACTT 7235 0.0 19.907139 12-13 GCAGAGT 14775 0.0 19.624672 9 CATGGGG 5205 0.0 19.328281 4 AGAGTAC 12545 0.0 18.706144 10-11 TACACTG 1575 0.0 18.680532 5 ATGGGGA 2935 0.0 18.270416 5 >>END_MODULE