FastQCFastQC Report
Fri 27 May 2016
SRR939248_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939248_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2091673
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67290.32170420519842247No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53910.25773627139615035No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37090.17732217225159No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53650.038.421041
GTATCAA83900.035.8902441
ATCAACG106700.028.0404933
TATCAAC107250.027.7640672
TCAACGC108400.027.6007464
CAACGCA108950.027.5042725
AACGCAG111950.026.792266
GTACATG97850.025.7740571
TACATGG99050.025.0840442
ACGCAGA126550.023.5514817
ACATGGG103900.023.3198683
CGCAGAG128250.023.2762438
GAGTACT61000.022.37189512-13
CATGGGG54550.022.0779974
GCAGAGT135600.021.524869
GTACTTT67400.020.14234514-15
AGTACTT64850.019.6919612-13
AGAGTAC116000.019.6474610-11
ATGGGGA31200.018.3882795
GTACACG3105.5952114E-918.3852121
Produced by FastQC (version 0.11.5)