##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939248_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2091673 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.774855343067486 33.0 31.0 34.0 30.0 34.0 2 31.854511197495977 33.0 31.0 34.0 30.0 34.0 3 31.900780380107214 34.0 31.0 34.0 30.0 34.0 4 35.40292818236885 37.0 35.0 37.0 33.0 37.0 5 35.44718079738085 37.0 35.0 37.0 33.0 37.0 6 35.53521941527189 37.0 35.0 37.0 33.0 37.0 7 35.44805856364738 37.0 35.0 37.0 33.0 37.0 8 35.429363002725566 37.0 35.0 37.0 33.0 37.0 9 37.04317166210971 39.0 37.0 39.0 33.0 39.0 10-11 36.94821465879227 39.0 37.0 39.0 33.0 39.0 12-13 36.83704216672491 39.0 37.0 39.0 33.0 39.0 14-15 38.06324984832715 40.0 38.0 41.0 33.0 41.0 16-17 37.99706694115189 40.0 38.0 41.0 33.0 41.0 18-19 37.93292092023945 40.0 38.0 41.0 33.0 41.0 20-21 37.83004633133382 40.0 38.0 41.0 32.0 41.0 22-23 37.751175016362495 40.0 38.0 41.0 32.0 41.0 24-25 37.77208961439001 40.0 38.0 41.0 32.0 41.0 26-27 37.68531672971827 40.0 38.0 41.0 32.0 41.0 28-29 37.54668416143441 40.0 37.5 41.0 32.0 41.0 30-31 37.36166480133367 40.0 37.0 41.0 31.5 41.0 32-33 37.170609602935066 40.0 37.0 41.0 31.0 41.0 34-35 36.867828766733616 40.0 36.0 41.0 30.0 41.0 36-37 36.61454299979012 40.0 36.0 41.0 30.0 41.0 38-39 36.29850770172967 39.0 35.5 41.0 29.5 41.0 40-41 36.17039374701495 39.0 35.0 41.0 29.5 41.0 42-43 35.99640335750378 39.0 35.0 41.0 28.5 41.0 44-45 35.955100534356944 39.0 35.0 41.0 28.0 41.0 46-47 35.76957488096849 39.0 35.0 41.0 27.0 41.0 48-49 35.56637103409567 39.0 35.0 41.0 26.5 41.0 50-51 34.42383704336194 38.0 34.0 40.0 24.5 40.5 52-53 34.5386288870201 38.0 34.0 39.5 24.5 40.5 54-55 35.033824120691904 39.0 35.0 40.5 26.0 41.0 56-57 34.92753384491744 39.0 35.0 41.0 25.0 41.0 58-59 34.722689684286216 38.0 34.0 40.0 25.0 41.0 60-61 34.43637843965094 38.0 34.0 40.0 23.5 41.0 62-63 34.07123173650948 37.5 34.0 40.0 23.0 41.0 64-65 33.69266730507111 37.0 33.5 40.0 22.0 41.0 66-67 33.33815276097172 36.5 33.0 39.5 21.5 41.0 68-69 32.928115914868144 36.0 33.0 39.0 20.0 41.0 70-71 32.52102073316432 35.5 32.0 39.0 19.5 40.5 72-73 32.08818323896709 35.0 32.0 38.0 18.0 40.0 74-75 31.64322243486434 35.0 31.5 37.0 14.5 39.5 76-77 31.195443312601924 35.0 31.0 37.0 11.0 39.0 78-79 30.76386748789127 35.0 31.0 36.0 7.5 39.0 80-81 30.3053283185278 34.5 30.5 36.0 4.5 37.5 82-83 29.870513220756784 34.0 30.0 35.0 2.0 37.0 84-85 29.45951637755997 34.0 29.5 35.0 2.0 36.5 86-87 29.12197222032316 34.0 29.0 35.0 2.0 36.0 88-89 28.713767878631124 34.0 29.0 35.0 2.0 36.0 90-91 28.32075257461372 34.0 29.0 35.0 2.0 35.5 92-93 27.90583781499307 34.0 28.0 35.0 2.0 35.0 94-95 27.437852857497326 34.0 27.0 35.0 2.0 35.0 96-97 26.86411953493687 33.5 25.5 35.0 2.0 35.0 98-99 26.122999388527745 33.0 24.5 35.0 2.0 35.0 100-101 23.662033931690086 31.0 10.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.289988150650867 1101 2 -0.019494107787206616 1101 3 0.17710537309599417 1101 4 0.2583299806399566 1101 5 0.21567674992170538 1101 6 0.24666530252843444 1101 7 0.4107367732469811 1101 8 0.2645045596383895 1101 9 0.28159403370958813 1101 10-11 0.3185340025651797 1101 12-13 0.21347266802396092 1101 14-15 0.19456320595607224 1101 16-17 0.3749910357217061 1101 18-19 0.4393463661470989 1101 20-21 0.45446438218767327 1101 22-23 0.4535187604095867 1101 24-25 0.48063883253278306 1101 26-27 0.3915489353023176 1101 28-29 0.3400209358409114 1101 30-31 0.40630192326872105 1101 32-33 0.46825472960851755 1101 34-35 0.4853100270104349 1101 36-37 0.4591778245656002 1101 38-39 0.5902948686136256 1101 40-41 0.5027536631601421 1101 42-43 0.3282162042237289 1101 44-45 0.6001323599785735 1101 46-47 0.4705794937139345 1101 48-49 0.33862542249089245 1101 50-51 0.37495417696192845 1101 52-53 0.4170573065333727 1101 54-55 0.3682297914689343 1101 56-57 0.2335088533145111 1101 58-59 0.3587499257852471 1101 60-61 0.5198493344191917 1101 62-63 0.528249931093093 1101 64-65 0.42984350717321007 1101 66-67 0.38027221412347245 1101 68-69 0.394485174327869 1101 70-71 0.291212463093629 1101 72-73 0.3177477422629451 1101 74-75 0.34345152221697717 1101 76-77 0.29928134292767794 1101 78-79 0.3073414150282687 1101 80-81 0.3308250513825399 1101 82-83 0.2531226081970921 1101 84-85 0.05033207419039343 1101 86-87 0.014064543103366134 1101 88-89 0.04447367042314099 1101 90-91 0.1867695379559251 1101 92-93 0.25305991633686276 1101 94-95 0.18748738158736344 1101 96-97 0.10104410420011334 1101 98-99 0.06997940638662925 1101 100-101 -0.03327712683166695 1102 1 0.36037385696630864 1102 2 0.2975610747755759 1102 3 0.36987201629237987 1102 4 0.30561316752675793 1102 5 0.3621190041876119 1102 6 0.4071905799031441 1102 7 0.35185036082452825 1102 8 0.42062302115312633 1102 9 0.3603864964954937 1102 10-11 0.39167675267639623 1102 12-13 0.42489232857864323 1102 14-15 0.49716617965736987 1102 16-17 0.5304450770922102 1102 18-19 0.6490648598692275 1102 20-21 0.5222135368864969 1102 22-23 0.5148852559316737 1102 24-25 0.46898392670227196 1102 26-27 0.5007728114843246 1102 28-29 0.4638232235933657 1102 30-31 0.480612619490806 1102 32-33 0.4567912692901288 1102 34-35 0.498537184462883 1102 36-37 0.5829661215199948 1102 38-39 0.6611374173144497 1102 40-41 0.633110782563314 1102 42-43 0.3839148561389578 1102 44-45 0.43622744829477256 1102 46-47 0.5407353512042334 1102 48-49 0.3558657975356567 1102 50-51 0.31051284665974777 1102 52-53 0.3473457282628516 1102 54-55 0.4557656464580262 1102 56-57 0.4383863881473147 1102 58-59 0.34375735803980234 1102 60-61 0.3361241730824531 1102 62-63 0.265852513623102 1102 64-65 0.3407733755854707 1102 66-67 0.3292486302063722 1102 68-69 0.31666392990062775 1102 70-71 0.4395584652373685 1102 72-73 0.23861264768800794 1102 74-75 0.12292984818658326 1102 76-77 0.01848045825239808 1102 78-79 0.05772330670979642 1102 80-81 0.2469535006139303 1102 82-83 0.1764257670908691 1102 84-85 0.16120914875449444 1102 86-87 0.09936572501172236 1102 88-89 -0.21271802806544926 1102 90-91 -0.3208985614826716 1102 92-93 -0.2925946274460536 1102 94-95 -0.22775793420779067 1102 96-97 -0.37651294256512813 1102 98-99 -0.23169217990147573 1102 100-101 -0.1636750765230417 1103 1 0.3335768170786899 1103 2 0.34651494657262205 1103 3 0.3392179944938043 1103 4 0.34115015813897287 1103 5 0.35931576128409404 1103 6 0.3013900255898889 1103 7 0.34256686130093783 1103 8 0.37734235234422187 1103 9 0.40056733930168065 1103 10-11 0.38092225143918057 1103 12-13 0.3417154862803997 1103 14-15 0.5335626445438137 1103 16-17 0.41158771814508555 1103 18-19 0.4718697735055173 1103 20-21 0.598483188670464 1103 22-23 0.5295205666445213 1103 24-25 0.5398843871909875 1103 26-27 0.5047082841965818 1103 28-29 0.5125508736881059 1103 30-31 0.5632725437976447 1103 32-33 0.6083410281131236 1103 34-35 0.4178667216446712 1103 36-37 0.6090078276072646 1103 38-39 0.7715777591194595 1103 40-41 0.6193405526105025 1103 42-43 0.39911796370191155 1103 44-45 0.514508847348452 1103 46-47 0.6916608278326208 1103 48-49 0.6626657359764394 1103 50-51 0.5279072772328135 1103 52-53 0.6548178843519068 1103 54-55 0.6040643443236604 1103 56-57 0.5232014455983744 1103 58-59 0.4989237206947763 1103 60-61 0.39122051072969555 1103 62-63 0.49867697427215774 1103 64-65 0.43772577399804646 1103 66-67 0.3333907019206208 1103 68-69 0.4343259860736879 1103 70-71 0.22699934834858482 1103 72-73 0.36248711062170713 1103 74-75 0.22195061937552651 1103 76-77 0.5082987997025192 1103 78-79 0.3550344080843111 1103 80-81 0.19675751188633228 1103 82-83 0.2800735710163629 1103 84-85 0.359860957112474 1103 86-87 0.34033767099771595 1103 88-89 0.23091884881920421 1103 90-91 0.17962326218382074 1103 92-93 0.11297154772803353 1103 94-95 0.09248243265821188 1103 96-97 -0.06934451784467655 1103 98-99 -0.1355951910609079 1103 100-101 -0.4088409542169913 1104 1 0.35862268409578846 1104 2 0.2913556933452064 1104 3 0.31230452692054556 1104 4 0.33272962952077023 1104 5 0.4745074793936368 1104 6 0.2703222307261939 1104 7 0.39470578316174 1104 8 0.4056124933510077 1104 9 0.48828247267400116 1104 10-11 0.4190704952442914 1104 12-13 0.4711577620873797 1104 14-15 0.4831619187007732 1104 16-17 0.5586536341672925 1104 18-19 0.4597454916204171 1104 20-21 0.5352054399615014 1104 22-23 0.609214224483928 1104 24-25 0.6089066536037464 1104 26-27 0.5649219421965768 1104 28-29 0.5949070265594258 1104 30-31 0.6274755282650872 1104 32-33 0.600325842149303 1104 34-35 0.6469852853747327 1104 36-37 0.7610372842018833 1104 38-39 0.8648144650446383 1104 40-41 0.5331442832617128 1104 42-43 0.6005766084469784 1104 44-45 0.7279475153515449 1104 46-47 0.7369568323504012 1104 48-49 0.5763985416996817 1104 50-51 0.3664422626262933 1104 52-53 0.4339584615737593 1104 54-55 0.5301358125633513 1104 56-57 0.47994981867420705 1104 58-59 0.509394916475685 1104 60-61 0.5194943987138103 1104 62-63 0.44437524357010005 1104 64-65 0.52346625031916 1104 66-67 0.6407862984193358 1104 68-69 0.5559837826636738 1104 70-71 0.47329325322085936 1104 72-73 0.44057866801268375 1104 74-75 0.25602161656663114 1104 76-77 0.31050175472942243 1104 78-79 0.2828827465005972 1104 80-81 0.1597625341741562 1104 82-83 0.24086546791025043 1104 84-85 0.20026514890953706 1104 86-87 0.29091316656892374 1104 88-89 0.1845178874518787 1104 90-91 0.19880109506595645 1104 92-93 0.16762509283695692 1104 94-95 -0.0011834359914963954 1104 96-97 -0.4312831236211778 1104 98-99 -0.6083550673571239 1104 100-101 -0.7864321563142305 1105 1 0.1838615795401779 1105 2 0.12529254276996227 1105 3 0.24946501619527695 1105 4 0.3223293808798644 1105 5 0.24467873608376323 1105 6 0.14699212831985164 1105 7 0.1779646646990969 1105 8 0.25600415545223854 1105 9 0.34538427848124087 1105 10-11 0.32950379942904107 1105 12-13 0.35208352802778364 1105 14-15 0.30837882101440073 1105 16-17 0.2690915476908273 1105 18-19 0.39640158753680055 1105 20-21 0.47811877912455003 1105 22-23 0.43124804912888237 1105 24-25 0.2940830200751563 1105 26-27 0.3035808616622049 1105 28-29 0.329078030394399 1105 30-31 0.27356154116069575 1105 32-33 0.3266048678140976 1105 34-35 0.27426835672203254 1105 36-37 0.43621744350063096 1105 38-39 0.5712321132809706 1105 40-41 0.5534208962668927 1105 42-43 0.2715980905996176 1105 44-45 0.46237822250313343 1105 46-47 0.4724972048903311 1105 48-49 0.420574707994092 1105 50-51 0.3645591501900327 1105 52-53 0.4255718355043925 1105 54-55 0.4709525610567553 1105 56-57 0.4371602731813482 1105 58-59 0.4539134907940223 1105 60-61 0.6576518221197745 1105 62-63 0.5754332316858992 1105 64-65 0.5218643075487108 1105 66-67 0.4666390260943345 1105 68-69 0.332354863408753 1105 70-71 0.33315736991350775 1105 72-73 0.4698455987725154 1105 74-75 0.38518727356861504 1105 76-77 0.3424822690789533 1105 78-79 0.38385347324845753 1105 80-81 0.47235919859322095 1105 82-83 0.38289434080839513 1105 84-85 0.3400558585896789 1105 86-87 0.3600812994443068 1105 88-89 0.45385801451159224 1105 90-91 0.36446204790846437 1105 92-93 0.49650628778828576 1105 94-95 0.6069209668489073 1105 96-97 0.43990692863555836 1105 98-99 0.2957774024056121 1105 100-101 0.16693898237682347 1106 1 0.1931672057139231 1106 2 0.2663871679514038 1106 3 0.14930966187877104 1106 4 0.21222156057626762 1106 5 0.3073676112590533 1106 6 0.2926920274497462 1106 7 0.27670050803060064 1106 8 0.3142871391572015 1106 9 0.25297818814099315 1106 10-11 0.34335075876936827 1106 12-13 0.39498561431683044 1106 14-15 0.40926520170474845 1106 16-17 0.4242892797535873 1106 18-19 0.34880043040085695 1106 20-21 0.31152997755163625 1106 22-23 0.3809530501949894 1106 24-25 0.32830709932789404 1106 26-27 0.3165519988701533 1106 28-29 0.35064183483760303 1106 30-31 0.23721857459544538 1106 32-33 0.30129200646538834 1106 34-35 0.275203250398377 1106 36-37 0.3238108303579921 1106 38-39 0.388760113961645 1106 40-41 0.4354395182828199 1106 42-43 0.42574150451969217 1106 44-45 0.611594480222152 1106 46-47 0.5378100867410893 1106 48-49 0.5286904974461919 1106 50-51 0.46792192276484457 1106 52-53 0.27895113786048853 1106 54-55 0.3793541235183113 1106 56-57 0.5726489960263805 1106 58-59 0.4912654147068025 1106 60-61 0.43022817177000405 1106 62-63 0.5660832698197211 1106 64-65 0.7103555818744027 1106 66-67 0.48527885318001296 1106 68-69 0.39613406345959845 1106 70-71 0.6225147791509187 1106 72-73 0.4384682297601401 1106 74-75 0.36308183047821885 1106 76-77 0.48337405206061135 1106 78-79 0.492496293634062 1106 80-81 0.5189913773875396 1106 82-83 0.6977352909966008 1106 84-85 0.7652661584117197 1106 86-87 0.7069336446742973 1106 88-89 0.5431689353408622 1106 90-91 0.6773610691403924 1106 92-93 0.6969403931606415 1106 94-95 0.6402136121469688 1106 96-97 0.5744626798718286 1106 98-99 0.6565842146231944 1106 100-101 0.6616450956793791 1107 1 0.11944318187219238 1107 2 0.1287765525108746 1107 3 0.2198781482862664 1107 4 0.2753539629801267 1107 5 0.22399365669691917 1107 6 0.11283845088281197 1107 7 0.14019644685097177 1107 8 0.05791227194002602 1107 9 0.2381608926265315 1107 10-11 0.21266133634049567 1107 12-13 0.320599429586494 1107 14-15 0.25566269951907117 1107 16-17 0.30404630041837066 1107 18-19 0.2732889904703697 1107 20-21 0.35499194463945827 1107 22-23 0.4462377830680637 1107 24-25 0.31517153498891304 1107 26-27 0.38126475367352697 1107 28-29 0.297279196804368 1107 30-31 0.38459526343174844 1107 32-33 0.41466116029955913 1107 34-35 0.4299012339203898 1107 36-37 0.5663388823820412 1107 38-39 0.5845529193658194 1107 40-41 0.4002678498120886 1107 42-43 0.4376847609486916 1107 44-45 0.5701630414334034 1107 46-47 0.5396539747451072 1107 48-49 0.6829058244899358 1107 50-51 0.6344505879828475 1107 52-53 0.5563587792119122 1107 54-55 0.6511910995567476 1107 56-57 0.5498874923035331 1107 58-59 0.5367382248944779 1107 60-61 0.3682491990076784 1107 62-63 0.4911124346726865 1107 64-65 0.5231025849873632 1107 66-67 0.4883148030290059 1107 68-69 0.5374972930330699 1107 70-71 0.4512300467699646 1107 72-73 0.44207935652371333 1107 74-75 0.5755746976693068 1107 76-77 0.33941643626303986 1107 78-79 0.5374922298707467 1107 80-81 0.4372275490887354 1107 82-83 0.5208283426872278 1107 84-85 0.47843878013822305 1107 86-87 0.4334137071354398 1107 88-89 0.36069452056338136 1107 90-91 0.41674613304470043 1107 92-93 0.29508599818154835 1107 94-95 0.31866351097734835 1107 96-97 0.39571725698822746 1107 98-99 0.7123714540372958 1107 100-101 0.41894910429952503 1108 1 0.0728731955103612 1108 2 0.21591250865644085 1108 3 0.1614775955565939 1108 4 0.2680813787319778 1108 5 0.25148466014958615 1108 6 -5.96235178676352E-4 1108 7 0.10965865377013984 1108 8 0.09494312225472612 1108 9 0.17388782407649472 1108 10-11 0.23239426896378035 1108 12-13 0.2129544494087483 1108 14-15 0.31545372467529376 1108 16-17 0.265748304063429 1108 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-0.4442246539165886 2316 92-93 -0.5111563756066708 2316 94-95 -0.7189380418611293 2316 96-97 -0.6588233887707879 2316 98-99 -0.6743121869082458 2316 100-101 -0.21061472789016378 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7554.0 3 2229.0 4 2204.0 5 2427.0 6 2840.0 7 3387.0 8 4190.0 9 4925.0 10 6064.0 11 7457.0 12 9065.0 13 12253.0 14 11493.0 15 12124.0 16 14786.0 17 18808.0 18 16288.0 19 12399.0 20 12592.0 21 13426.0 22 14444.0 23 15659.0 24 18081.0 25 21017.0 26 23565.0 27 27847.0 28 32414.0 29 38436.0 30 46395.0 31 55940.0 32 69827.0 33 89852.0 34 121729.0 35 175532.0 36 271872.0 37 423857.0 38 403030.0 39 65649.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.3692960939283 18.80733153183908 14.765860085650887 23.057512288581737 2 15.21528324879122 19.973062670930947 40.77449128748754 24.037162792790294 3 19.19080468133321 24.27839658258272 29.607197102241535 26.92360163384254 4 12.793251990994866 16.441594212819243 36.96766586996555 33.797487926220334 5 14.119262884066144 37.21402385372574 33.676704176077536 14.990009086130582 6 32.11950071540271 35.57955273338369 17.71304577859781 14.587900772615791 7 29.12905341627744 32.56339095502293 20.241426475294976 18.066129153404653 8 26.42714465812017 35.03993761168537 19.664614965062064 18.8683027651324 9 26.73994198638503 17.13454077817471 20.115022540915962 36.010494694524304 10-11 25.26795929463359 26.5041995379109 27.004919653473834 21.222921513981678 12-13 26.07959506364208 23.80780832502075 27.638556983645145 22.474039627692022 14-15 22.910423037869013 25.196030708547468 26.090735888073347 25.80281036551017 16-17 22.581407525963744 28.51516107604255 26.421987010772053 22.481444387221654 18-19 22.365575964605707 26.877845914262856 28.98624153327425 21.77033658785719 20-21 23.237226833523554 26.08917922132229 28.794631056155595 21.87896288899856 22-23 22.870443517343116 25.95495876132287 28.745776725950005 22.428820995384005 24-25 22.637641025420166 26.164134890561662 28.909526239766365 22.288697844251804 26-27 22.370346514659353 26.520057194155783 28.749633922948675 22.35996236823619 28-29 22.285902678239683 26.527451699550426 29.03290481211315 22.153740810096746 30-31 22.383279862770852 26.24619904446789 28.857811367339174 22.512709725422084 32-33 22.203039062277597 26.379231854390646 28.76260325235499 22.655125830976772 34-35 22.784457052153932 26.53633694598846 28.36502194527436 22.314184056583247 36-37 22.811495434332265 26.254928804951046 28.43437593423557 22.49919982648111 38-39 22.492221865893477 26.434526352349536 28.556851797971678 22.51639998378531 40-41 22.620064569075048 26.269977586444238 28.61618236662783 22.493775477852886 42-43 22.587527343111887 26.300409496008786 28.25013918930297 22.861923971576356 44-45 22.597116825682125 26.599992850721872 28.09284849072261 22.710041832873387 46-47 22.62619942144178 26.80588308824464 27.778712157328293 22.78920533298528 48-49 22.921188707993906 26.718239733176567 27.723962519945772 22.636609038883755 50-51 22.70859276214607 26.93733639489324 27.58945345397223 22.764617388988462 52-53 22.953405867586998 26.928065746304146 27.439705281807235 22.67882310430162 54-55 22.56537662299029 26.82844151189453 27.578923832869606 23.027258032245573 56-57 22.672596218303884 27.092873577223 27.568457708173288 22.666072496299826 58-59 22.804444025579837 26.574345390159195 27.671346999030938 22.949863585230034 60-61 22.812484419404697 26.719067005189057 27.877807862975136 22.59064071243111 62-63 22.628851166354266 26.777093275238308 27.862417068500733 22.731638489906693 64-65 22.43886543773671 26.567643936645418 27.824744068906078 23.1687465567118 66-67 22.427938854839635 26.93996562306456 27.597461107582337 23.03463441451347 68-69 22.425032938708803 26.805365229682998 27.872051415584508 22.897550416023694 70-71 22.548942904304845 26.7772776863939 27.680658079089106 22.993121330212148 72-73 22.623084249012177 26.73944586802947 27.618200015308968 23.019269867649385 74-75 22.51634080246604 27.042357346876138 27.349412537383056 23.09188931327476 76-77 22.526895135363382 26.858739359507105 27.430950758629958 23.183414746499555 78-79 22.63188534560364 26.936342477934055 27.413917213822753 23.01785496263955 80-81 22.52391047071618 27.281730754931406 27.294611251269462 22.899747523082954 82-83 22.69840881437383 27.11104138118981 27.261768363598677 22.92878144083768 84-85 22.68348079644716 26.853089408014707 27.236423928922132 23.227005866615997 86-87 22.63193841534521 26.82113048979223 27.396837330791058 23.150093764071507 88-89 22.619640888385558 26.70778363087347 27.432005249790958 23.240570230950013 90-91 22.519283475489466 26.928203552863362 27.525523305766082 23.026989665881086 92-93 22.68184187462743 26.5544140925353 27.471962854562015 23.291781178275254 94-95 22.549404989887183 26.617451262158664 27.422253195833534 23.41089055212062 96-97 22.733319493659952 26.530509879966836 27.40651320628833 23.32965742008489 98-99 22.602158546839547 26.494439495801547 27.255061373837126 23.648340583521787 100-101 22.629689447788824 26.14455010186681 27.760863953353354 23.46489649699102 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1861.0 1 1079.5 2 368.0 3 440.5 4 380.5 5 368.5 6 621.0 7 1335.0 8 2132.0 9 2502.5 10 2324.5 11 2044.5 12 2195.5 13 2466.5 14 2713.0 15 2878.0 16 2997.0 17 3079.5 18 3188.0 19 3373.5 20 3580.5 21 3988.0 22 4685.0 23 5645.0 24 6797.5 25 8105.0 26 10232.0 27 13046.0 28 15371.5 29 18010.0 30 21454.5 31 25344.0 32 29344.0 33 33036.5 34 37181.5 35 41011.5 36 45356.5 37 50587.0 38 53854.0 39 55373.5 40 57747.0 41 62930.5 42 68197.5 43 71691.5 44 74940.0 45 77595.5 46 76795.0 47 77211.0 48 83750.5 49 85671.0 50 81554.5 51 78576.0 52 75448.5 53 73664.0 54 72415.0 55 67253.5 56 59627.0 57 53393.0 58 47882.5 59 41559.0 60 35919.5 61 30340.5 62 25223.0 63 20450.5 64 16118.0 65 12760.5 66 9686.0 67 8047.5 68 6529.0 69 4524.0 70 3302.0 71 2584.5 72 2199.0 73 1895.0 74 1384.5 75 1051.0 76 784.0 77 483.0 78 312.0 79 201.0 80 138.0 81 108.0 82 73.5 83 46.5 84 31.5 85 23.0 86 24.0 87 22.0 88 11.5 89 9.0 90 12.0 91 13.5 92 9.0 93 4.5 94 5.5 95 6.0 96 6.5 97 5.0 98 2.5 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.286708295225879 2 0.1137367074107664 3 0.12478049867259365 4 0.1263103745183879 5 0.13266892100247027 6 0.059378306264889394 7 0.06836632685893063 8 0.07352965783848622 9 0.07046990614689772 10-11 0.06614322602051086 12-13 0.0701830544258113 14-15 0.06576075705906229 16-17 0.06705158980395119 18-19 0.07197587768260144 20-21 0.07546590695581958 22-23 0.08443002323977028 24-25 0.09248577574028062 26-27 0.09317900073290615 28-29 0.08660531545800897 30-31 0.0830913818747003 32-33 0.07589618453744922 34-35 0.24473232670689923 36-37 0.3705168064032953 38-39 0.34152087826347616 40-41 0.3238555931065707 42-43 0.3481184678484639 44-45 0.36078775219644754 46-47 0.3681024710841513 48-49 0.3775924821900938 50-51 0.37137736156655465 52-53 0.3721422994894517 54-55 0.35760847895440634 56-57 0.3333934128326942 58-59 0.318955209538011 60-61 0.2746605229402493 62-63 0.3476642859567437 64-65 0.37811837701208556 66-67 0.38287533472010205 68-69 0.3948035854552791 70-71 0.39566414061853833 72-73 0.3790028364854353 74-75 0.38287533472010205 76-77 0.36587937024573153 78-79 0.3433854144505379 80-81 0.34068422741030746 82-83 0.3557439427673446 84-85 0.36298694872477677 86-87 0.3562937418994269 88-89 0.371974969318818 90-91 0.3959988009598059 92-93 0.39140917342242304 94-95 0.3910267044609745 96-97 0.39635736561116386 98-99 0.3848115838374354 100-101 0.3623415323523323 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2091673.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.08982713909761 #Duplication Level Percentage of deduplicated Percentage of total 1 74.41386337000057 38.017914163257565 2 13.82120732062226 14.122461857284435 3 4.856419045736101 7.443408286850365 4 2.2378164919543706 4.573186309718825 5 1.1919985129817272 3.0449498989148926 6 0.7512030181793129 2.30272994070897 7 0.48386775823587963 1.7304504088517636 8 0.34105766753447114 1.3939661823039964 9 0.2533055781243244 1.1647204379766827 >10 1.4569723105134318 13.73258987750663 >50 0.12557448553363118 4.457334522919111 >100 0.06352529394494254 6.028074824321274 >500 0.0021573639029470494 0.7119211508378329 >1k 8.441858750662314E-4 0.6954840914182429 >5k 1.875968611258292E-4 0.5808080471294084 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6729 0.32170420519842247 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5391 0.25773627139615035 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3709 0.17732217225159 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 9.561724036214073E-5 14-15 0.0 0.0 0.0 0.0 1.434258605432111E-4 16-17 0.0 0.0 0.0 0.0 1.9123448072428146E-4 18-19 0.0 0.0 0.0 0.0 1.9123448072428146E-4 20-21 0.0 0.0 0.0 4.7808620181070365E-5 1.9123448072428146E-4 22-23 0.0 0.0 0.0 4.063732715390981E-4 1.9123448072428146E-4 24-25 0.0 0.0 0.0 5.019905119012389E-4 1.9123448072428146E-4 26-27 0.0 0.0 0.0 9.800767137119425E-4 1.9123448072428146E-4 28-29 0.0 0.0 0.0 0.0021991965283292367 1.9123448072428146E-4 30-31 0.0 0.0 0.0 0.0064541637244445 1.9123448072428146E-4 32-33 0.0 0.0 0.0 0.013744978302057731 1.9123448072428146E-4 34-35 0.0 0.0 0.0 0.025673229037234787 1.9123448072428146E-4 36-37 0.0 0.0 0.0 0.04780862018107036 1.9123448072428146E-4 38-39 0.0 0.0 0.0 0.07988820432256857 2.3904310090535185E-4 40-41 0.0 0.0 0.0 0.11486020998502156 2.3904310090535185E-4 42-43 0.0 0.0 0.0 0.1543501302545857 2.3904310090535185E-4 44-45 0.0 0.0 0.0 0.20125038665221573 2.6294741099588704E-4 46-47 0.0 0.0 0.0 0.2606047886070146 2.868517210864222E-4 48-49 0.0 0.0 0.0 0.32799103875223323 3.5856465135802775E-4 50-51 0.0 0.0 0.0 0.39803066731750136 3.824689614485629E-4 52-53 0.0 0.0 0.0 0.47378342599440737 3.824689614485629E-4 54-55 0.0 0.0 0.0 0.5565879561480211 3.824689614485629E-4 56-57 0.0 0.0 0.0 0.6557191300934706 4.063732715390981E-4 58-59 0.0 0.0 0.0 0.7633841427412411 4.302775816296333E-4 60-61 0.0 0.0 0.0 0.8871845647001229 4.302775816296333E-4 62-63 0.0 0.0 0.0 1.012036776302988 4.302775816296333E-4 64-65 0.0 0.0 0.0 1.1488172386410302 4.302775816296333E-4 66-67 0.0 0.0 0.0 1.3096215326200606 4.302775816296333E-4 68-69 0.0 0.0 0.0 1.4960034383959635 4.302775816296333E-4 70-71 0.0 0.0 0.0 1.7050944387578748 4.302775816296333E-4 72-73 0.0 0.0 0.0 1.9206396028442305 4.302775816296333E-4 74-75 0.0 0.0 0.0 2.148543295247393 4.302775816296333E-4 76-77 0.0 0.0 0.0 2.399634168438374 4.302775816296333E-4 78-79 0.0 0.0 0.0 2.6856731429817184 4.302775816296333E-4 80-81 0.0 0.0 0.0 2.997194112081573 4.302775816296333E-4 82-83 0.0 0.0 0.0 3.325185150833806 4.780862018107037E-4 84-85 0.0 0.0 0.0 3.665080536011126 5.258948219917741E-4 86-87 0.0 0.0 0.0 4.015661147798915 5.258948219917741E-4 88-89 0.0 0.0 0.0 4.418257538343708 5.258948219917741E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5365 0.0 38.42104 1 GTATCAA 8390 0.0 35.890244 1 ATCAACG 10670 0.0 28.040493 3 TATCAAC 10725 0.0 27.764067 2 TCAACGC 10840 0.0 27.600746 4 CAACGCA 10895 0.0 27.504272 5 AACGCAG 11195 0.0 26.79226 6 GTACATG 9785 0.0 25.774057 1 TACATGG 9905 0.0 25.084044 2 ACGCAGA 12655 0.0 23.551481 7 ACATGGG 10390 0.0 23.319868 3 CGCAGAG 12825 0.0 23.276243 8 GAGTACT 6100 0.0 22.371895 12-13 CATGGGG 5455 0.0 22.077997 4 GCAGAGT 13560 0.0 21.52486 9 GTACTTT 6740 0.0 20.142345 14-15 AGTACTT 6485 0.0 19.69196 12-13 AGAGTAC 11600 0.0 19.64746 10-11 ATGGGGA 3120 0.0 18.388279 5 GTACACG 310 5.5952114E-9 18.385212 1 >>END_MODULE