FastQCFastQC Report
Fri 27 May 2016
SRR939248_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939248_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2091673
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70800.33848503088197823No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60390.28871625727348393No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42290.20218265474574657No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57250.037.9105571
GTATCAA90150.034.6345021
TAGACCG1153.896681E-628.8842435
ATCAACG116100.026.5260873
TCAACGC118600.025.966944
CAACGCA119800.025.6672275
TATCAAC120900.025.6299442
AACGCAG122750.025.0503776
GTACATG94650.024.7911761
TACATGG97300.023.5068782
ACATGGG99300.022.5555553
ACGCAGA137800.022.2800317
CGCAGAG138750.022.1274858
GAGTACT70750.021.02824412-13
AGTACTT74550.020.43380712-13
AGAGTAC125350.020.42485810-11
GCAGAGT148500.020.420019
GTACTTT74200.019.98655914-15
CATGGGG51750.019.3478574
ATAGTGC4800.018.7833546
Produced by FastQC (version 0.11.5)