##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939248_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2091673 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.29892865663036 33.0 31.0 34.0 31.0 34.0 2 32.50016661304133 34.0 31.0 34.0 31.0 34.0 3 32.586041890869176 34.0 31.0 34.0 31.0 34.0 4 36.11940967828145 37.0 35.0 37.0 35.0 37.0 5 36.055416883996685 37.0 35.0 37.0 35.0 37.0 6 36.0922792425011 37.0 35.0 37.0 35.0 37.0 7 36.04018410143459 37.0 35.0 37.0 35.0 37.0 8 36.01564489286805 37.0 35.0 37.0 35.0 37.0 9 37.72748464984728 39.0 38.0 39.0 35.0 39.0 10-11 37.64732226308797 39.0 37.0 39.0 35.0 39.0 12-13 37.56738266449871 39.0 37.0 39.0 35.0 39.0 14-15 38.87017043295009 40.0 38.0 41.0 35.0 41.0 16-17 38.811098101854355 40.0 38.0 41.0 35.0 41.0 18-19 38.92775711117369 40.0 38.0 41.0 35.0 41.0 20-21 38.932522674433336 40.0 38.0 41.0 35.0 41.0 22-23 38.87953590260045 40.0 38.0 41.0 35.0 41.0 24-25 38.80993850377186 40.0 38.0 41.0 34.0 41.0 26-27 38.64908998681916 40.0 38.0 41.0 34.0 41.0 28-29 38.50123202814207 40.0 38.0 41.0 34.0 41.0 30-31 38.30980965954048 40.0 38.0 41.0 34.0 41.0 32-33 38.118801074546546 40.0 37.5 41.0 33.5 41.0 34-35 38.07319093376451 40.0 38.0 41.0 33.0 41.0 36-37 37.992249266496245 40.0 37.5 41.0 33.0 41.0 38-39 37.88750846810185 40.0 37.0 41.0 33.0 41.0 40-41 37.7120458121322 40.0 37.0 41.0 33.0 41.0 42-43 37.537911996760485 40.0 37.0 41.0 33.0 41.0 44-45 37.31483554073701 40.0 37.0 41.0 32.0 41.0 46-47 37.02331172224339 40.0 36.0 41.0 31.0 41.0 48-49 36.711031552255065 40.0 36.0 41.0 30.5 41.0 50-51 36.46856607127405 39.0 36.0 41.0 30.0 41.0 52-53 36.239753776044346 39.0 35.0 41.0 30.0 41.0 54-55 35.955561170412395 39.0 35.0 40.5 29.0 41.0 56-57 35.71205131012353 38.5 35.0 40.0 28.5 41.0 58-59 35.418776022829576 38.0 35.0 40.0 28.0 41.0 60-61 35.08841176417155 38.0 34.0 40.0 28.0 41.0 62-63 34.70857036448814 37.5 34.0 40.0 27.0 41.0 64-65 34.28785928775674 37.0 34.0 39.5 26.0 41.0 66-67 33.91199962900511 36.0 33.0 39.0 26.0 40.5 68-69 33.526293306840984 36.0 33.0 39.0 26.0 40.0 70-71 33.126196350959255 35.0 33.0 38.5 25.0 40.0 72-73 32.630325342441196 35.0 32.0 37.5 23.5 40.0 74-75 32.12730622807676 35.0 32.0 37.0 22.0 39.0 76-77 30.134840866617296 33.5 29.5 35.0 20.0 37.5 78-79 31.120161229790696 34.0 31.0 36.0 20.0 38.0 80-81 31.16689893687971 34.5 31.0 36.0 20.0 37.0 82-83 30.860290064460365 34.0 31.0 35.0 19.5 37.0 84-85 30.48540426730182 34.0 31.0 35.0 18.0 36.5 86-87 29.948283264162228 34.0 30.0 35.0 11.5 36.0 88-89 29.45008278062584 34.0 30.0 35.0 7.0 36.0 90-91 29.03830785213559 34.0 29.0 35.0 2.0 35.0 92-93 28.67647261307097 34.0 29.0 35.0 2.0 35.0 94-95 28.271013203306637 34.0 29.0 35.0 2.0 35.0 96-97 27.712433300998768 33.5 28.0 35.0 2.0 35.0 98-99 26.943120411268872 33.0 26.5 35.0 2.0 35.0 100-101 23.527006850497187 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15059871192990926 1101 2 0.18190451021466458 1101 3 0.18057480762244182 1101 4 0.18683791885383982 1101 5 0.218868400258998 1101 6 0.10796611545580248 1101 7 0.1358300718797807 1101 8 0.20409062966130875 1101 9 0.14154615436390827 1101 10-11 0.18005855541152727 1101 12-13 0.2655736508934581 1101 14-15 0.25902507229356075 1101 16-17 0.270876967360266 1101 18-19 0.27868986333803747 1101 20-21 0.2934955479022463 1101 22-23 0.24831541864075035 1101 24-25 0.33703872181084193 1101 26-27 0.35725523349334765 1101 28-29 0.2532886975311328 1101 30-31 0.2510624771947221 1101 32-33 0.2856403284261262 1101 34-35 0.3543579225857698 1101 36-37 0.43334949890386554 1101 38-39 0.43128378904087583 1101 40-41 0.531842515524481 1101 42-43 0.4682573126520708 1101 44-45 0.4207309076717749 1101 46-47 0.4194328695111622 1101 48-49 0.3723480632865801 1101 50-51 0.5279733210105064 1101 52-53 0.47760962983555544 1101 54-55 0.38634547721807166 1101 56-57 0.41601312952013814 1101 58-59 0.4027141931871654 1101 60-61 0.5638206647279702 1101 62-63 0.6021746221133952 1101 64-65 0.6083667725164759 1101 66-67 0.5370554617401382 1101 68-69 0.6310102597121059 1101 70-71 0.6295136584632033 1101 72-73 0.38352473236845697 1101 74-75 0.24281741379192567 1101 76-77 0.4399368785706841 1101 78-79 0.5517865615129018 1101 80-81 0.5631135682698734 1101 82-83 0.4265006133331859 1101 84-85 0.48404501143302525 1101 86-87 0.6222231456179053 1101 88-89 0.7559282231067179 1101 90-91 0.7880015530722666 1101 92-93 0.7713734009004689 1101 94-95 1.0339924885814291 1101 96-97 0.8353714855508869 1101 98-99 0.7272493851506105 1101 100-101 0.5070267773547243 1102 1 0.21075800144863166 1102 2 0.1890152619430978 1102 3 0.15397458868072533 1102 4 0.04592145876559073 1102 5 0.10708106724202793 1102 6 0.10490727015919532 1102 7 0.18085560080333352 1102 8 0.21799862503120693 1102 9 0.28940347791852616 1102 10-11 0.2937709080298916 1102 12-13 0.24954819106731918 1102 14-15 0.368700223094379 1102 16-17 0.2859955950514177 1102 18-19 0.30137551409003294 1102 20-21 0.23731521279692913 1102 22-23 0.17815142364545267 1102 24-25 0.23062085721777947 1102 26-27 0.39026937676254647 1102 28-29 0.30948944778054965 1102 30-31 0.3025508982389411 1102 32-33 0.2637487268452219 1102 34-35 0.4200224483748016 1102 36-37 0.4326579537429964 1102 38-39 0.3667846396242851 1102 40-41 0.3046746413662831 1102 42-43 0.3020250745412554 1102 44-45 0.38925104100122354 1102 46-47 0.35805609503540126 1102 48-49 0.30529916230659637 1102 50-51 0.34148526258492495 1102 52-53 0.36772390324772175 1102 54-55 0.3036448705884922 1102 56-57 0.3809303468246412 1102 58-59 0.3493828414076816 1102 60-61 0.2534219612466586 1102 62-63 0.1884440243522718 1102 64-65 0.19312773830411345 1102 66-67 0.22165033676805024 1102 68-69 0.25862561098678327 1102 70-71 0.15961771573753225 1102 72-73 0.349536515475684 1102 74-75 0.32539966969104483 1102 76-77 0.5682404914515828 1102 78-79 0.40576760026969794 1102 80-81 0.4103213476104308 1102 82-83 0.37216706357077456 1102 84-85 0.34073269855995036 1102 86-87 0.4347899945075788 1102 88-89 0.4488718927274391 1102 90-91 0.5479918394610515 1102 92-93 0.5636687255183936 1102 94-95 0.6099889332055675 1102 96-97 0.3926757956874738 1102 98-99 0.4491398731782006 1102 100-101 0.18744125902991726 1103 1 0.1935172166191066 1103 2 0.22230797628675703 1103 3 0.18771694318611054 1103 4 0.08415909359106166 1103 5 0.09853986643928891 1103 6 0.11232465088190935 1103 7 0.12085162580489595 1103 8 0.12067591823107193 1103 9 0.1785781107007125 1103 10-11 0.284032161858768 1103 12-13 0.2268649101406126 1103 14-15 0.27092913759182125 1103 16-17 0.2819589252703594 1103 18-19 0.3276235388550006 1103 20-21 0.37436113299925466 1103 22-23 0.37607410852422873 1103 24-25 0.24183200672396765 1103 26-27 0.28517075447233964 1103 28-29 0.3058157979554821 1103 30-31 0.29965444750887826 1103 32-33 0.30755500369853195 1103 34-35 0.3604903993191684 1103 36-37 0.29667404211466675 1103 38-39 0.29336392360316665 1103 40-41 0.3473350926827834 1103 42-43 0.3429681190929088 1103 44-45 0.41489763000124213 1103 46-47 0.2711118344369723 1103 48-49 0.3891019687451944 1103 50-51 0.3119721606480823 1103 52-53 0.23737955569269786 1103 54-55 0.3399189739221882 1103 56-57 0.30312662036172355 1103 58-59 0.3198778688752597 1103 60-61 0.1790509458812437 1103 62-63 0.22122038135309907 1103 64-65 0.3624183804906238 1103 66-67 0.21631296227975128 1103 68-69 0.06353714484561124 1103 70-71 0.16275580600588313 1103 72-73 0.21191088464090768 1103 74-75 0.36941394716779996 1103 76-77 0.5110278455743256 1103 78-79 0.4691967176098011 1103 80-81 0.411643076639443 1103 82-83 0.4025082434613161 1103 84-85 0.4450312753254977 1103 86-87 0.26061582285884555 1103 88-89 0.4760205994373088 1103 90-91 0.6810068365302584 1103 92-93 0.41622011106324663 1103 94-95 0.4419985752398574 1103 96-97 0.25316764708083994 1103 98-99 0.2630107115188274 1103 100-101 0.2983075535941211 1104 1 0.10900253716632591 1104 2 0.13557749502766825 1104 3 0.17801120134482318 1104 4 0.14058723392606964 1104 5 0.1401476408819562 1104 6 0.0885021719620056 1104 7 0.1983399198462621 1104 8 0.23859463291079663 1104 9 0.15335907980287544 1104 10-11 0.20258906706961 1104 12-13 0.17645561977797541 1104 14-15 0.3022155953466594 1104 16-17 0.2265290867516967 1104 18-19 0.2018348335828648 1104 20-21 0.23277905211539718 1104 22-23 0.23080593869516264 1104 24-25 0.1733202659719808 1104 26-27 0.23481699966649927 1104 28-29 0.29646708952517997 1104 30-31 0.2907446707082926 1104 32-33 0.3153984978084168 1104 34-35 0.3063674529638476 1104 36-37 0.3331414989474837 1104 38-39 0.4023274962925143 1104 40-41 0.4613557465570892 1104 42-43 0.49901527212615093 1104 44-45 0.48661209962329366 1104 46-47 0.38856900180240217 1104 48-49 0.34632339770852383 1104 50-51 0.3357242499224995 1104 52-53 0.22406667907129219 1104 54-55 0.2049950041067703 1104 56-57 0.3793191293511171 1104 58-59 0.2501238713188201 1104 60-61 0.1872886755341483 1104 62-63 0.18122019400423284 1104 64-65 0.2794568231397889 1104 66-67 0.3203146002441244 1104 68-69 0.3283611908049622 1104 70-71 0.3140400155743279 1104 72-73 0.18824684933367308 1104 74-75 0.10676635975282522 1104 76-77 0.16300524594822008 1104 78-79 0.36288975184513106 1104 80-81 0.3868592303939806 1104 82-83 0.5066875952074774 1104 84-85 0.4384147824695077 1104 86-87 0.37518366924348 1104 88-89 0.6169425432085625 1104 90-91 0.5936692111236113 1104 92-93 0.5263463172888478 1104 94-95 0.5707020274103591 1104 96-97 0.5318180393759846 1104 98-99 0.5779897886650751 1104 100-101 0.27431244607594607 1105 1 -0.013958291355734787 1105 2 0.024126317144066434 1105 3 0.043053185836370744 1105 4 -0.011013918672020395 1105 5 0.08918494755312167 1105 6 0.01094622775870846 1105 7 0.09035206742938584 1105 8 0.07215536029079317 1105 9 -0.0014846767818994522 1105 10-11 0.007091600888983862 1105 12-13 0.007988369788506589 1105 14-15 0.18135043562648434 1105 16-17 0.09157423193269665 1105 18-19 0.12188519390146979 1105 20-21 0.07406674333710583 1105 22-23 0.09126057753370986 1105 24-25 0.11823195104088313 1105 26-27 0.08761526339443293 1105 28-29 0.06013443749601777 1105 30-31 0.07280986519604937 1105 32-33 0.048908390766399634 1105 34-35 0.12762361630827712 1105 36-37 0.012901102175597146 1105 38-39 -0.01590309707425064 1105 40-41 0.13958610287215123 1105 42-43 0.16620204780149805 1105 44-45 0.24332197993852134 1105 46-47 0.09493739596144479 1105 48-49 0.15176347103652432 1105 50-51 0.2709486852126446 1105 52-53 0.2499265182975563 1105 54-55 0.2519378272345847 1105 56-57 0.27485491889135005 1105 58-59 0.28139560105879724 1105 60-61 0.07760998595599489 1105 62-63 0.04033088134882945 1105 64-65 -0.06515946916077553 1105 66-67 0.019181361380290696 1105 68-69 0.07748791864853644 1105 70-71 0.17939859579262674 1105 72-73 0.13421657519653962 1105 74-75 0.26485588101372315 1105 76-77 0.3651833560666411 1105 78-79 0.34536478239846247 1105 80-81 0.3877500180377922 1105 82-83 0.3230917051573279 1105 84-85 0.25743647006696335 1105 86-87 0.29316509492513987 1105 88-89 0.5932911040412989 1105 90-91 0.6030201108665416 1105 92-93 0.421205587591011 1105 94-95 0.5396051891533737 1105 96-97 0.5464400472566382 1105 98-99 0.7260862634427703 1105 100-101 0.3776111523003962 1106 1 -0.016206854161730178 1106 2 -0.022561986023909242 1106 3 0.02896135508493103 1106 4 0.11614827923020954 1106 5 0.05439936828808101 1106 6 0.04262087655852298 1106 7 0.07736632250774989 1106 8 0.028201518615858845 1106 9 0.1822470456366858 1106 10-11 0.13773543202587035 1106 12-13 0.12205013940282328 1106 14-15 0.10260473593237407 1106 16-17 0.12081041764850653 1106 18-19 0.041409505237460564 1106 20-21 0.1019245503143722 1106 22-23 0.14782177309050581 1106 24-25 0.2179676539333144 1106 26-27 0.15508137349866047 1106 28-29 0.16794910302550647 1106 30-31 0.08913872634792597 1106 32-33 0.2157994695024925 1106 34-35 0.22310937148094467 1106 36-37 0.12497314741953858 1106 38-39 0.25304925820175583 1106 40-41 0.127038973774809 1106 42-43 0.13306970294053855 1106 44-45 0.15843194270679106 1106 46-47 0.12346356990908447 1106 48-49 -0.06339380022205177 1106 50-51 0.14145835090614867 1106 52-53 0.21472871960313 1106 54-55 0.18946217342407579 1106 56-57 0.1626848482002643 1106 58-59 0.31853596939870243 1106 60-61 0.265255422403726 1106 62-63 0.29564450127355 1106 64-65 0.2285160571269671 1106 66-67 0.3293296476135694 1106 68-69 0.30616796051372575 1106 70-71 0.1921731695549127 1106 72-73 0.20974165843488635 1106 74-75 0.3287630907661452 1106 76-77 0.387309557487022 1106 78-79 0.4851457384502602 1106 80-81 0.5237416819025107 1106 82-83 0.5708642850948387 1106 84-85 0.4825752946021815 1106 86-87 0.4596963626081596 1106 88-89 0.575096555947578 1106 90-91 0.6576411713968078 1106 92-93 0.644964623871445 1106 94-95 0.6403185837605143 1106 96-97 0.6745877532817808 1106 98-99 0.872783843790323 1106 100-101 0.6051214238296048 1107 1 0.030320836638914272 1107 2 0.09266852642857515 1107 3 0.06568261783140628 1107 4 0.021942051669803675 1107 5 0.07689364543156785 1107 6 0.07204163711095646 1107 7 0.07461556198237673 1107 8 0.10344794655190981 1107 9 0.034011097854630634 1107 10-11 0.10528228956678731 1107 12-13 0.1940488785658161 1107 14-15 0.16499869730066763 1107 16-17 0.05366666377452134 1107 18-19 0.1588702277285634 1107 20-21 0.11402664169454368 1107 22-23 0.14855283217637094 1107 24-25 0.08654840274353148 1107 26-27 0.11151396756253718 1107 28-29 0.031989706701665455 1107 30-31 0.23052619332293034 1107 32-33 0.09499201707977534 1107 34-35 0.25764274736711457 1107 36-37 0.2562590313846371 1107 38-39 0.20336196989453015 1107 40-41 0.23506790215932227 1107 42-43 0.23428865968895707 1107 44-45 0.1975316696484768 1107 46-47 0.21698143065942332 1107 48-49 0.2694330903506241 1107 50-51 0.19691549413590792 1107 52-53 0.22958981468583772 1107 54-55 0.09570193865838661 1107 56-57 0.2893082927311852 1107 58-59 0.528616784523436 1107 60-61 0.3649524671878055 1107 62-63 0.3753103794659651 1107 64-65 0.3012537294555102 1107 66-67 0.3831377863115506 1107 68-69 0.5029794749773018 1107 70-71 0.4356643199505683 1107 72-73 0.45238117789948973 1107 74-75 0.42353858968137104 1107 76-77 0.2783412760024575 1107 78-79 0.5317908022512192 1107 80-81 0.5823490721692117 1107 82-83 0.6047223356154241 1107 84-85 0.46443329197273187 1107 86-87 0.4894537400671801 1107 88-89 0.5837931078634782 1107 90-91 0.5778418029472121 1107 92-93 0.5768590019507975 1107 94-95 0.5943342044582245 1107 96-97 0.6859681360457159 1107 98-99 0.8912088211584965 1107 100-101 0.8967029007461242 1108 1 -0.017446635902309993 1108 2 0.03239805661782924 1108 3 0.02108964466324892 1108 4 -0.03958300645116708 1108 5 0.029029265124741244 1108 6 -0.004464082105783973 1108 7 0.10077663074471843 1108 8 -0.018322465919759168 1108 9 -0.011890485556655506 1108 10-11 0.0583867499777071 1108 12-13 -0.06804599247814735 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>>Per sequence quality scores pass #Quality Count 2 8.0 3 6.0 4 21.0 5 45.0 6 105.0 7 224.0 8 593.0 9 1067.0 10 1810.0 11 2704.0 12 3964.0 13 5611.0 14 7011.0 15 9334.0 16 12145.0 17 17004.0 18 16359.0 19 11403.0 20 10529.0 21 11251.0 22 11761.0 23 13016.0 24 14813.0 25 18046.0 26 21529.0 27 26121.0 28 31989.0 29 39554.0 30 48230.0 31 58940.0 32 73229.0 33 93536.0 34 127335.0 35 182059.0 36 286819.0 37 475941.0 38 419353.0 39 38208.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.554716460099606 19.484055375186255 15.232059540406665 22.729168624307476 2 15.763027968520893 20.52562709371876 39.724421551552275 23.986923386208073 3 19.182348292491223 24.473758565511915 29.926522931643714 26.41737021035315 4 13.152390454913363 17.089191283723604 36.556622378354554 33.20179588300849 5 14.051957452240382 37.43763963105132 33.65349172647923 14.856911190229066 6 32.208476181506384 35.941325436624176 17.336648701780824 14.51354968008862 7 29.04560129618731 32.72208418811162 20.13871193059336 18.09360258510771 8 26.47024654427341 35.438330943699135 19.376164438705285 18.71525807332217 9 26.50380819564052 17.117924264452427 19.961628801442675 36.41663873846438 10-11 25.292457281802655 26.70505858229274 26.826803233583835 21.175680902320774 12-13 25.998232037225705 23.918963432620682 27.60620326408573 22.47660126606788 14-15 22.808744987739175 25.361310677221162 26.024623963901682 25.805320371137984 16-17 22.472815258499168 28.746801883001567 26.434189487251874 22.346193371247388 18-19 22.302147611027152 26.987679240493133 29.013258764634813 21.69691438384489 20-21 23.117188967874043 26.1310921927089 28.918119610474484 21.833599228942575 22-23 22.765819513853263 26.010232000891154 28.912956279494928 22.310992205760652 24-25 22.57645496606185 26.247034370820483 29.008102367842003 22.168408295275672 26-27 22.29911808669299 26.60924138435504 28.864451456998808 22.227189071953156 28-29 22.188410903616386 26.616062835825677 29.089633035374074 22.10589322518386 30-31 22.32251408322429 26.31795218468661 28.95457368336255 22.404960048726547 32-33 22.161638076314986 26.429776547290135 28.878677498825102 22.529907877569773 34-35 22.76660222619633 26.541927701569307 28.52950225294626 22.161967819288108 36-37 22.7984223706616 26.292308052785934 28.546856672049444 22.362412904503024 38-39 22.45217471129834 26.534542597987397 28.647384986701052 22.365897704013207 40-41 22.609198147660678 26.286501492013464 28.73466594845363 22.369634411872223 42-43 22.55198929220551 26.348556496453245 28.402764347481224 22.696689863860023 44-45 22.609456230416253 26.56481126455663 28.21139976038148 22.614332744645633 46-47 22.6310393386342 26.87126061621053 27.833051222431436 22.664648822723834 48-49 22.883500432428967 26.751863221450005 27.79994769736952 22.564688648751503 50-51 22.716337591965857 26.95105783743444 27.63531871377601 22.6972858568237 52-53 22.992169426100542 26.978404368178012 27.42586914876274 22.603557056958714 54-55 22.574250372787716 26.911544012854783 27.486227531741335 23.027978082616162 56-57 22.68578788367015 27.16928028425093 27.5146019478188 22.63032988426011 58-59 22.83263807265181 26.694663548571317 27.641877043680346 22.830821335096523 60-61 22.779864540630758 26.80587591409257 27.81915578974897 22.595103755527703 62-63 22.56555204661224 26.921680337362154 27.790424688384398 22.72234292764121 64-65 22.44424019534607 26.64659500949599 27.732831023977223 23.17633377118072 66-67 22.400561655669886 26.979121497480723 27.63127888537071 22.98903796147868 68-69 22.420551871751837 26.762287528562794 27.79825722445317 23.018903375232203 70-71 22.527230594839633 26.773663952252573 27.692999814024468 23.006105638883326 72-73 22.60709489485211 26.820970581921742 27.54570145524659 23.026233067979554 74-75 22.519868067331746 27.041224895095933 27.24924020150377 23.189666836068547 76-77 22.44995075945454 26.865401987353206 27.43901240957591 23.24563484361634 78-79 22.562226504812177 26.91900215760303 27.38532743884919 23.133443898735607 80-81 22.377535619025945 27.268915578091736 27.293656822918184 23.059891979964135 82-83 22.617086896469953 27.03696992789982 27.272116626260413 23.073826549369812 84-85 22.520107110432654 26.832994449897285 27.255861695398853 23.39103674427121 86-87 22.4294719944788 26.74044540527295 27.39802637167934 23.43205622856891 88-89 22.379261731698282 26.690009462517487 27.392260697155297 23.538468108628933 90-91 22.324437684996113 26.82338957862933 27.47989260058738 23.372280135787175 92-93 22.312043995404636 26.4768441338584 27.47640286029413 23.734709010442838 94-95 22.198546331094775 26.531776238446447 27.42739902460853 23.842278405850244 96-97 22.278625769898067 26.43764106530992 27.460506494083926 23.823226670708088 98-99 22.09336736669642 26.41447778883219 27.279287919287576 24.212866925183814 100-101 22.029616997514665 26.03361762763303 27.93826752349378 23.99849785135852 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 581.0 1 392.5 2 277.0 3 362.5 4 311.0 5 300.5 6 523.5 7 1265.5 8 2099.0 9 2383.5 10 2160.0 11 1853.0 12 1950.5 13 2177.5 14 2331.0 15 2507.5 16 2596.0 17 2707.5 18 2876.5 19 3027.0 20 3347.0 21 3840.0 22 4442.5 23 5477.0 24 6762.0 25 8137.0 26 10209.5 27 12838.0 28 15235.0 29 18010.0 30 21415.0 31 25238.0 32 29442.5 33 33147.5 34 37373.0 35 41248.5 36 45422.5 37 50439.0 38 54092.5 39 56153.0 40 58428.5 41 63488.0 42 68529.5 43 72296.0 44 75538.5 45 78391.5 46 77769.5 47 77946.0 48 84421.5 49 86309.5 50 82245.0 51 79106.5 52 75653.5 53 73910.5 54 73306.5 55 68046.0 56 59751.5 57 53278.0 58 47855.0 59 41712.0 60 35814.5 61 29701.5 62 24732.0 63 20080.0 64 15774.0 65 12347.5 66 9254.0 67 7735.0 68 6018.5 69 4087.0 70 3050.0 71 2445.0 72 2081.5 73 1726.0 74 1320.0 75 1075.0 76 719.5 77 403.5 78 273.0 79 161.5 80 89.5 81 55.5 82 37.0 83 19.5 84 8.5 85 3.0 86 3.5 87 3.5 88 2.5 89 2.0 90 1.5 91 0.5 92 0.0 93 0.5 94 1.0 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2786764470354592 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.002486048249415659 16-17 5.976077522633795E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.3904310090535182E-5 26-27 0.0013147370549794351 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0037529766842140234 36-37 0.020748941158584538 38-39 0.007362527507884837 40-41 0.01776090239726764 42-43 0.005378469770370416 44-45 7.171293027160555E-4 46-47 7.171293027160555E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 5.497991320823092E-4 60-61 0.0023665266989629833 62-63 0.0016015887760658573 64-65 2.3904310090535182E-5 66-67 0.0 68-69 4.5418189172016847E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.2379913112613683 78-79 0.0 80-81 0.0011474068843456888 82-83 0.0 84-85 0.0 86-87 0.10864508936148241 88-89 0.3662618392071801 90-91 0.36700287281998667 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 1.1952155045267591E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2091673.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.40420443993033 #Duplication Level Percentage of deduplicated Percentage of total 1 72.97666336658669 36.05353996306824 2 14.545628532263255 14.372304114304352 3 5.128893206088609 7.601666655125141 4 2.326194702824408 4.5969519466168025 5 1.316711437229908 3.2525540516650437 6 0.8004103323814173 2.372618141808248 7 0.5276910164322037 1.8249108399851863 8 0.3560785252557852 1.407342100672457 9 0.275018481015177 1.2228362334749614 >10 1.537533026919422 14.130640478352316 >50 0.13422347118520306 4.559476518340038 >100 0.07204977816751701 6.595156988412901 >500 0.0017424741902369127 0.5858121754108403 >1k 9.680412167982815E-4 0.7967696009751472 >5k 1.9360824335965627E-4 0.6274201917882926 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7080 0.33848503088197823 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6039 0.28871625727348393 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4229 0.20218265474574657 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 7.171293027160555E-5 14-15 0.0 0.0 0.0 0.0 9.561724036214073E-5 16-17 0.0 0.0 0.0 0.0 1.1952155045267591E-4 18-19 0.0 0.0 0.0 0.0 1.434258605432111E-4 20-21 0.0 0.0 0.0 4.7808620181070365E-5 1.434258605432111E-4 22-23 0.0 0.0 0.0 4.063732715390981E-4 1.434258605432111E-4 24-25 0.0 0.0 0.0 5.019905119012389E-4 1.434258605432111E-4 26-27 0.0 0.0 0.0 9.800767137119425E-4 1.434258605432111E-4 28-29 0.0 0.0 0.0 0.002247005148510307 1.434258605432111E-4 30-31 0.0 0.0 0.0 0.0064063551042634285 1.434258605432111E-4 32-33 0.0 0.0 0.0 0.013721073991967196 1.434258605432111E-4 34-35 0.0 0.0 0.0 0.025840559207868534 1.434258605432111E-4 36-37 0.0 0.0 0.0 0.047832524491160905 1.434258605432111E-4 38-39 0.0 0.0 0.0 0.08034238621428876 1.434258605432111E-4 40-41 0.0 0.0 0.0 0.11574466945837136 1.434258605432111E-4 42-43 0.0 0.0 0.0 0.15521068541784494 1.434258605432111E-4 44-45 0.0 0.0 0.0 0.20203922888520337 1.434258605432111E-4 46-47 0.0 0.0 0.0 0.26182390842163183 1.434258605432111E-4 48-49 0.0 0.0 0.0 0.3299272878695666 1.434258605432111E-4 50-51 0.0 0.0 0.0 0.40042109832655487 1.434258605432111E-4 52-53 0.0 0.0 0.0 0.4766519432052716 1.9123448072428146E-4 54-55 0.0 0.0 0.0 0.5597194207698812 1.9123448072428146E-4 56-57 0.0 0.0 0.0 0.659304776607051 1.9123448072428146E-4 58-59 0.0 0.0 0.0 0.7681411004492575 1.9123448072428146E-4 60-61 0.0 0.0 0.0 0.8928020775713985 1.9123448072428146E-4 62-63 0.0 0.0 0.0 1.0190646434696053 1.9123448072428146E-4 64-65 0.0 0.0 0.0 1.1565622351103637 1.9123448072428146E-4 66-67 0.0 0.0 0.0 1.31808365839211 1.9123448072428146E-4 68-69 0.0 0.0 0.0 1.5049675546799142 1.9123448072428146E-4 70-71 0.0 0.0 0.0 1.7146561627940888 1.9123448072428146E-4 72-73 0.0 0.0 0.0 1.9309662648033417 1.9123448072428146E-4 74-75 0.0 0.0 0.0 2.159658799439492 1.9123448072428146E-4 76-77 0.0 0.0 0.0 2.4117536536542756 1.9123448072428146E-4 78-79 0.0 0.0 0.0 2.6986770876709696 1.9123448072428146E-4 80-81 0.0 0.0 0.0 3.011871358477162 1.9123448072428146E-4 82-83 0.0 0.0 0.0 3.341129325664193 2.3904310090535185E-4 84-85 0.0 0.0 0.0 3.681741840144229 2.3904310090535185E-4 86-87 0.0 0.0 0.0 4.033350337265911 2.6294741099588704E-4 88-89 0.0 0.0 0.0 4.437213656245503 2.868517210864222E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5725 0.0 37.910557 1 GTATCAA 9015 0.0 34.634502 1 TAGACCG 115 3.896681E-6 28.884243 5 ATCAACG 11610 0.0 26.526087 3 TCAACGC 11860 0.0 25.96694 4 CAACGCA 11980 0.0 25.667227 5 TATCAAC 12090 0.0 25.629944 2 AACGCAG 12275 0.0 25.050377 6 GTACATG 9465 0.0 24.791176 1 TACATGG 9730 0.0 23.506878 2 ACATGGG 9930 0.0 22.555555 3 ACGCAGA 13780 0.0 22.280031 7 CGCAGAG 13875 0.0 22.127485 8 GAGTACT 7075 0.0 21.028244 12-13 AGTACTT 7455 0.0 20.433807 12-13 AGAGTAC 12535 0.0 20.424858 10-11 GCAGAGT 14850 0.0 20.42001 9 GTACTTT 7420 0.0 19.986559 14-15 CATGGGG 5175 0.0 19.347857 4 ATAGTGC 480 0.0 18.783354 6 >>END_MODULE