FastQCFastQC Report
Fri 27 May 2016
SRR939247_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939247_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2111933
Sequences flagged as poor quality0
Sequence length101
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120760.571798442469529No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85000.40247488911816803No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63200.2992519175560967No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA132900.038.1006281
GGTATCA81550.034.7992551
ATCAACG172500.028.9970533
TATCAAC174450.028.9175282
TCAACGC173350.028.8815364
CAACGCA176200.028.413715
AACGCAG181200.027.6139376
GTACATG142950.024.7987121
ACGCAGA203150.024.5603287
CGCAGAG204950.024.2758438
TACATGG141500.024.124962
CATGGGG41250.024.0216334
ACATGGG141400.022.968453
GCAGAGT213850.022.7764429
AGTACTT103400.022.68039512-13
GAGTACT98150.022.6144112-13
GTACTTT107550.020.48807714-15
AGAGTAC197350.020.28573210-11
TACTTTT122500.018.9934614-15
ATGGGGG22750.018.7556025
Produced by FastQC (version 0.11.5)