##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939247_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2111933 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.979629088612185 33.0 31.0 34.0 30.0 34.0 2 32.032301687600885 33.0 31.0 34.0 30.0 34.0 3 32.073713512691924 34.0 31.0 34.0 30.0 34.0 4 35.57669064312173 37.0 35.0 37.0 33.0 37.0 5 35.62288955189393 37.0 35.0 37.0 33.0 37.0 6 35.72058677997834 37.0 35.0 37.0 35.0 37.0 7 35.66117627784593 37.0 35.0 37.0 33.0 37.0 8 35.648895111729395 37.0 35.0 37.0 33.0 37.0 9 37.28153449943724 39.0 37.0 39.0 34.0 39.0 10-11 37.18676965604496 39.0 37.0 39.0 34.0 39.0 12-13 37.088848699272184 39.0 37.0 39.0 33.0 39.0 14-15 38.30867906320892 40.0 38.0 41.0 33.0 41.0 16-17 38.2479089062011 40.0 38.0 41.0 33.0 41.0 18-19 38.210636653719604 40.0 38.0 41.0 33.5 41.0 20-21 38.20046800727107 40.0 38.0 41.0 33.5 41.0 22-23 38.19929538484412 40.0 38.0 41.0 33.5 41.0 24-25 38.11818343669046 40.0 38.0 41.0 33.0 41.0 26-27 38.02788156631863 40.0 38.0 41.0 33.0 41.0 28-29 37.87641274604829 40.0 38.0 41.0 33.0 41.0 30-31 37.667377942387375 40.0 37.5 41.0 33.0 41.0 32-33 37.41525985909591 40.0 37.0 41.0 32.0 41.0 34-35 37.12110682488507 40.0 36.0 41.0 31.0 41.0 36-37 36.86939145323265 40.0 36.0 41.0 30.5 41.0 38-39 36.27906567111741 39.0 35.5 40.5 29.5 41.0 40-41 36.233469764429074 39.0 35.0 40.0 30.0 41.0 42-43 36.34992303259621 39.0 35.0 41.0 30.0 41.0 44-45 36.183538019435275 40.0 35.0 41.0 29.5 41.0 46-47 35.82719290810835 39.5 35.0 41.0 27.5 41.0 48-49 35.521415688849984 39.0 35.0 41.0 26.5 41.0 50-51 34.379025044828595 38.0 34.0 40.0 24.5 40.5 52-53 34.46774779313548 38.0 34.5 39.5 24.0 40.5 54-55 34.94565831397114 39.0 35.0 40.5 24.5 41.0 56-57 34.86623107835334 39.0 35.0 41.0 24.5 41.0 58-59 34.66852760007065 38.5 35.0 40.5 24.0 41.0 60-61 34.376976210893055 38.0 34.0 40.0 23.0 41.0 62-63 33.99789434608011 38.0 34.0 40.0 22.5 41.0 64-65 33.626239563471 37.0 34.0 40.0 21.5 41.0 66-67 33.27173968113571 36.5 33.0 39.5 20.0 41.0 68-69 32.871617849619284 36.0 33.0 39.0 18.5 41.0 70-71 32.46747600421036 35.5 32.5 39.0 17.5 40.0 72-73 32.03310403312984 35.0 32.0 38.0 13.5 40.0 74-75 31.57422536605091 35.0 31.5 37.0 9.5 39.5 76-77 31.102321427810445 35.0 31.0 37.0 7.0 39.0 78-79 30.705180277972833 35.0 31.0 36.0 4.0 39.0 80-81 30.30287253430862 35.0 31.0 36.0 2.0 37.5 82-83 29.870881794072066 34.0 30.0 35.5 2.0 37.0 84-85 29.452611422805553 34.0 30.0 35.0 2.0 37.0 86-87 29.011981203949176 34.0 29.0 35.0 2.0 36.0 88-89 28.57804130149962 34.0 29.0 35.0 2.0 36.0 90-91 28.168456338340278 34.0 29.0 35.0 2.0 35.5 92-93 27.723226305001155 34.0 27.5 35.0 2.0 35.0 94-95 27.248014496672006 34.0 26.5 35.0 2.0 35.0 96-97 26.645949705790855 33.5 25.5 35.0 2.0 35.0 98-99 25.865889211447524 33.0 24.0 35.0 2.0 35.0 100-101 23.13769234156576 30.0 9.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3222573551068777 1101 2 0.4253920609519426 1101 3 0.3979869564194445 1101 4 0.1872992958140003 1101 5 0.2749151470479987 1101 6 0.29997667448321863 1101 7 0.3412947660425516 1101 8 0.20554095076079193 1101 9 0.36182079303840453 1101 10-11 0.44285683583176905 1101 12-13 0.49836697448496636 1101 14-15 0.5824781537739767 1101 16-17 0.5405459644592554 1101 18-19 0.559544248625933 1101 20-21 0.5945934347468196 1101 22-23 0.6215098187380903 1101 24-25 0.6366754009531661 1101 26-27 0.6092442962318003 1101 28-29 0.5865269276626464 1101 30-31 0.5072104799288013 1101 32-33 0.5527870311540752 1101 34-35 0.5117271868088196 1101 36-37 0.4723703186054564 1101 38-39 0.8866564039855405 1101 40-41 0.6645610353770977 1101 42-43 0.7011761355807238 1101 44-45 0.601112044989847 1101 46-47 0.7751515528185564 1101 48-49 0.6700933866491923 1101 50-51 0.65148218037519 1101 52-53 0.6525352667090445 1101 54-55 0.6028132137657849 1101 56-57 0.6759504030480201 1101 58-59 0.5994824749745149 1101 60-61 0.5167729181802798 1101 62-63 0.5983773200373435 1101 64-65 0.42151911957829924 1101 66-67 0.461799099777906 1101 68-69 0.5630203908749394 1101 70-71 0.3722439251279184 1101 72-73 0.3262928642508953 1101 74-75 0.31871104723665056 1101 76-77 0.40556349147139414 1101 78-79 0.48467831166826514 1101 80-81 0.33409262440852316 1101 82-83 0.39742655234462987 1101 84-85 0.5130296751404124 1101 86-87 0.6968509596068486 1101 88-89 0.6130271521864401 1101 90-91 0.6953202119758863 1101 92-93 0.7421710351678392 1101 94-95 0.80405616410048 1101 96-97 0.8056849204920553 1101 98-99 0.8113459799768172 1101 100-101 0.5294518305574165 1102 1 0.38682528212552114 1102 2 0.35619787070429254 1102 3 0.4350047343298229 1102 4 0.2810185631021014 1102 5 0.2991577779457302 1102 6 0.36412945681925635 1102 7 0.41775498276455636 1102 8 0.3298939743410614 1102 9 0.46154104633064463 1102 10-11 0.5303703862849147 1102 12-13 0.48512113125962486 1102 14-15 0.6451353070635264 1102 16-17 0.5495356511969334 1102 18-19 0.5727554254232956 1102 20-21 0.5378422189355163 1102 22-23 0.6133843774159828 1102 24-25 0.5099257053499784 1102 26-27 0.5565656753304964 1102 28-29 0.5520244548381399 1102 30-31 0.5680504872598959 1102 32-33 0.41404161486568114 1102 34-35 0.3132989683364471 1102 36-37 0.46017895992300595 1102 38-39 0.9177672498890104 1102 40-41 0.7705233768385753 1102 42-43 0.6030121775839277 1102 44-45 0.7255415052023011 1102 46-47 0.7603644743033584 1102 48-49 0.5520124291738213 1102 50-51 0.4028873783503215 1102 52-53 0.5635469723115065 1102 54-55 0.5364933534544392 1102 56-57 0.5055376310266695 1102 58-59 0.5723243122383721 1102 60-61 0.39015023809599825 1102 62-63 0.4965929935250557 1102 64-65 0.49828280962065463 1102 66-67 0.6263624803797683 1102 68-69 0.5000174257587489 1102 70-71 0.5413989025308084 1102 72-73 0.5772856169783935 1102 74-75 0.5100221343843536 1102 76-77 0.6573250148619998 1102 78-79 0.6079795501406089 1102 80-81 0.4010221307955284 1102 82-83 0.3370258425353079 1102 84-85 0.3584586082526755 1102 86-87 0.31479651910736806 1102 88-89 0.3638481356048082 1102 90-91 0.41805797891803564 1102 92-93 0.3340221513842003 1102 94-95 0.11484779471548023 1102 96-97 0.09634231815150329 1102 98-99 0.13599622555436142 1102 100-101 -0.10174085084817008 1103 1 0.31277136181070375 1103 2 0.3056360823432769 1103 3 0.23671041105869506 1103 4 0.2772855667916332 1103 5 0.2764588868111346 1103 6 0.3149150912196603 1103 7 0.29964903120156094 1103 8 0.3046281258241521 1103 9 0.4349344620139135 1103 10-11 0.4743215280000186 1103 12-13 0.4953722433849279 1103 14-15 0.600047214703217 1103 16-17 0.5600864227887143 1103 18-19 0.5689814714730588 1103 20-21 0.6285454198168665 1103 22-23 0.5793577733392823 1103 24-25 0.6510134901100599 1103 26-27 0.5892302289173017 1103 28-29 0.5859072892405521 1103 30-31 0.4730906173881664 1103 32-33 0.5835305972605838 1103 34-35 0.3449993456482545 1103 36-37 0.07552277841212174 1103 38-39 0.7256566943529563 1103 40-41 0.7015958484565985 1103 42-43 0.5722279140106536 1103 44-45 0.48906588502840975 1103 46-47 0.45931370187570053 1103 48-49 0.41872414924423396 1103 50-51 0.3327712425448297 1103 52-53 0.35429480866149987 1103 54-55 0.4746494070771874 1103 56-57 0.4403359940584721 1103 58-59 0.34535629764742026 1103 60-61 0.35490577113878174 1103 62-63 0.2786306444623108 1103 64-65 0.25558288200701185 1103 66-67 0.32586923337511564 1103 68-69 0.2667699362948923 1103 70-71 0.24187233941173503 1103 72-73 0.3810095023036837 1103 74-75 0.3047938498741978 1103 76-77 0.28197917356700586 1103 78-79 0.20569611992992165 1103 80-81 0.23355135699705087 1103 82-83 0.2502700386705783 1103 84-85 0.32798528839357033 1103 86-87 0.16724912944641446 1103 88-89 0.1348957651605076 1103 90-91 0.13715961906254037 1103 92-93 0.13712498515134897 1103 94-95 0.21532566170846934 1103 96-97 0.24966515295513148 1103 98-99 0.3373414750532575 1103 100-101 -0.2242342850877499 1104 1 0.3222501985264046 1104 2 0.36231423589179457 1104 3 0.36407715135368335 1104 4 0.2798512223104126 1104 5 0.4040663766035735 1104 6 0.3479436551219166 1104 7 0.33168760726005075 1104 8 0.29538356104094277 1104 9 0.34850377303850877 1104 10-11 0.3963046561025081 1104 12-13 0.4871182062425703 1104 14-15 0.4300356954335385 1104 16-17 0.4855938860231319 1104 18-19 0.3366034170312773 1104 20-21 0.41171514065541004 1104 22-23 0.49083699007212545 1104 24-25 0.49001766166188787 1104 26-27 0.5144132741069498 1104 28-29 0.5578305538469195 1104 30-31 0.4262491860190494 1104 32-33 0.5393479364064504 1104 34-35 0.4309560927878664 1104 36-37 0.4890092305850189 1104 38-39 0.8570365560353537 1104 40-41 0.6956051804685117 1104 42-43 0.5366825462253146 1104 44-45 0.6143719499724796 1104 46-47 0.6222565783895675 1104 48-49 0.6376601768127514 1104 50-51 0.6886842877128814 1104 52-53 0.6592569472943453 1104 54-55 0.7916179822504006 1104 56-57 0.853866709591891 1104 58-59 0.7250182551243611 1104 60-61 0.6599437512613022 1104 62-63 0.5395626149159796 1104 64-65 0.4350716186011496 1104 66-67 0.5585552420507298 1104 68-69 0.5575348719402768 1104 70-71 0.6985628003336686 1104 72-73 0.6535570962121895 1104 74-75 0.5682871574700066 1104 76-77 0.4979692585742477 1104 78-79 0.49893683488035734 1104 80-81 0.4809230850170536 1104 82-83 0.5884261890105442 1104 84-85 0.5814197473117737 1104 86-87 0.39288538826399844 1104 88-89 0.4545390348625631 1104 90-91 0.5619529307608069 1104 92-93 0.39993922802117154 1104 94-95 0.29478082292426677 1104 96-97 0.33360521820580047 1104 98-99 0.45261082299997923 1104 100-101 0.3071252262447466 1105 1 0.20805579183987533 1105 2 0.32172366990862855 1105 3 0.3092925918405456 1105 4 0.1747772466288069 1105 5 0.21191759066411464 1105 6 0.2958136264901725 1105 7 0.2421886009015708 1105 8 0.3442930343076469 1105 9 0.383500199234291 1105 10-11 0.43843216447925215 1105 12-13 0.34849333587044384 1105 14-15 0.39545345868656767 1105 16-17 0.40154183242846386 1105 18-19 0.3753213509179716 1105 20-21 0.4956814642644076 1105 22-23 0.5547130596311689 1105 24-25 0.42599698730776225 1105 26-27 0.5099837193657706 1105 28-29 0.5874858986467189 1105 30-31 0.5322362376925369 1105 32-33 0.46689783538968044 1105 34-35 0.31756475915981497 1105 36-37 0.2887429511837638 1105 38-39 0.650234656341496 1105 40-41 0.6764383313243698 1105 42-43 0.4559558864721325 1105 44-45 0.4110901441477921 1105 46-47 0.31965503483666424 1105 48-49 0.32530400693929806 1105 50-51 0.14598094238423442 1105 52-53 0.18139542497768701 1105 54-55 0.3670484913832297 1105 56-57 0.2142946778118855 1105 58-59 0.22638167176382495 1105 60-61 0.2885227199398983 1105 62-63 0.2107276957500659 1105 64-65 0.301296173321667 1105 66-67 0.2030608795719573 1105 68-69 0.19226057730180912 1105 70-71 0.18817449514318696 1105 72-73 0.1642563017121219 1105 74-75 0.15442978324519174 1105 76-77 0.20552465253968322 1105 78-79 0.22436279929105396 1105 80-81 0.18886684614645688 1105 82-83 0.09576281657883001 1105 84-85 0.18647719673108298 1105 86-87 0.27577409628212735 1105 88-89 0.3918089583046509 1105 90-91 0.4274095307302339 1105 92-93 0.29398729465505724 1105 94-95 0.15934810340386107 1105 96-97 0.3360345787633996 1105 98-99 0.3424792946099231 1105 100-101 0.23906284786771081 1106 1 0.13732872674661678 1106 2 0.22899259055783006 1106 3 0.2903017425680119 1106 4 0.16836259162260347 1106 5 0.18768694882783876 1106 6 0.2261230011657389 1106 7 0.21374669640332655 1106 8 0.14928020549200482 1106 9 0.21264187978442095 1106 10-11 0.4141204465055637 1106 12-13 0.45277541656096787 1106 14-15 0.46989803770861727 1106 16-17 0.484343186004466 1106 18-19 0.522555492870417 1106 20-21 0.43302938471092745 1106 22-23 0.47800835968702415 1106 24-25 0.46807958115091 1106 26-27 0.5845899562959573 1106 28-29 0.6379665466625823 1106 30-31 0.5947884551743314 1106 32-33 0.40909388638853983 1106 34-35 0.397525976092858 1106 36-37 0.353588412155986 1106 38-39 0.7239145736833876 1106 40-41 0.7302670777587466 1106 42-43 0.6700835270082237 1106 44-45 0.5204492471688695 1106 46-47 0.6251281684788097 1106 48-49 0.4965130795568271 1106 50-51 0.29209583814702 1106 52-53 0.5002333913508679 1106 54-55 0.3896850478226028 1106 56-57 0.3309266564156914 1106 58-59 0.5458513151771385 1106 60-61 0.5087030154723067 1106 62-63 0.45159612499877255 1106 64-65 0.6846199488989697 1106 66-67 0.41947805584828757 1106 68-69 0.5218606593666806 1106 70-71 0.3839532215557213 1106 72-73 0.4156823024339005 1106 74-75 0.4946923661134086 1106 76-77 0.580425655609698 1106 78-79 0.6646158656004566 1106 80-81 0.6222390696595248 1106 82-83 0.4254153015130022 1106 84-85 0.5049248606012817 1106 86-87 0.6319136362244855 1106 88-89 0.602056932637133 1106 90-91 0.48171843081378185 1106 92-93 0.46484067043801147 1106 94-95 0.6197328937182291 1106 96-97 0.9628210963949861 1106 98-99 0.9166967121372025 1106 100-101 0.7426682641491844 1107 1 0.14983001130113394 1107 2 0.15598972249792808 1107 3 0.27103739526183546 1107 4 0.19712330013616253 1107 5 0.1475226659829474 1107 6 0.18436260094526347 1107 7 0.1698037212816459 1107 8 0.29338970559009425 1107 9 0.31092501428428676 1107 10-11 0.40197148022161855 1107 12-13 0.37521880274009334 1107 14-15 0.34238217929922143 1107 16-17 0.3036440847255193 1107 18-19 0.3858847311996598 1107 20-21 0.38070644303436296 1107 22-23 0.34461262814491533 1107 24-25 0.41517683827668606 1107 26-27 0.4415077494780064 1107 28-29 0.5548475782299249 1107 30-31 0.45730375305084436 1107 32-33 0.41833076758360477 1107 34-35 0.41180414987673686 1107 36-37 0.49784738768872927 1107 38-39 0.9683357534182591 1107 40-41 0.6195336905519753 1107 42-43 0.48431090249986397 1107 44-45 0.6102681016265024 1107 46-47 0.6354313467002441 1107 48-49 0.5425509295395372 1107 50-51 0.48044399446095554 1107 52-53 0.5105849155331939 1107 54-55 0.4864268336803619 1107 56-57 0.41428501909915383 1107 58-59 0.5698713168587304 1107 60-61 0.5360124109375946 1107 62-63 0.5901458395941148 1107 64-65 0.5565840794657575 1107 66-67 0.5215215485286606 1107 68-69 0.5659499569255644 1107 70-71 0.5881575684881923 1107 72-73 0.5261821342740305 1107 74-75 0.49449010716590536 1107 76-77 0.58970808010125 1107 78-79 0.7142517527838699 1107 80-81 0.5968116633385847 1107 82-83 0.6227809137550402 1107 84-85 0.5154175690027323 1107 86-87 0.6487849752441122 1107 88-89 0.6498295151055338 1107 90-91 0.7557067857432038 1107 92-93 0.7343657823110341 1107 94-95 0.6733278946991597 1107 96-97 0.6876619284126271 1107 98-99 0.9244635958546539 1107 100-101 0.7979991653926319 1108 1 0.08471044441986209 1108 2 0.12358411532057545 1108 3 0.1138666814499345 1108 4 0.14529610247559788 1108 5 0.035437842205759296 1108 6 0.056248284414579075 1108 7 0.08924409443658732 1108 8 0.14339863505698247 1108 9 0.16168085186828307 1108 10-11 0.35899138281565257 1108 12-13 0.26534160270420415 1108 14-15 0.37115507700359274 1108 16-17 0.35536670205343057 1108 18-19 0.3830503952440836 1108 20-21 0.3318046354991324 1108 22-23 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1488.0 5 1744.0 6 2103.0 7 2541.0 8 3223.0 9 3903.0 10 4782.0 11 6284.0 12 8456.0 13 11930.0 14 12412.0 15 13885.0 16 18422.0 17 24001.0 18 20558.0 19 13035.0 20 12590.0 21 12987.0 22 13901.0 23 15210.0 24 17245.0 25 19882.0 26 22735.0 27 26321.0 28 31214.0 29 37179.0 30 45268.0 31 55439.0 32 69185.0 33 89217.0 34 122491.0 35 179643.0 36 274592.0 37 431235.0 38 417296.0 39 60902.0 40 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.40825597659469 19.631489832605478 14.902363091039916 22.057891099759917 2 14.964587118534716 20.941370689614278 41.038904359128836 23.055137832722167 3 18.87155942847411 26.114875394395842 30.24178226360799 24.771782913522056 4 12.353726041723013 17.01622477245746 37.94662726486796 32.68342192095157 5 13.230788650958313 38.387676035618085 33.60261080349499 14.77892450992861 6 30.26179191842005 36.66077596137164 18.284145628094873 14.793286492113438 7 27.629238920430975 34.16212500148163 20.847867057024665 17.360769021062737 8 26.180542021534624 34.67266794791366 20.212501120261294 18.934288910290423 9 27.383397919950532 16.908510827979566 20.85853728857604 34.84955396349386 10-11 24.66622651103965 27.30343831084549 27.162169690662257 20.8681654874526 12-13 25.942216043303002 24.97197559880009 27.886608140765578 21.19920021713133 14-15 22.87759400435657 25.82652994455187 27.104553916125095 24.191322134966462 16-17 21.3530955049695 29.28025992661358 27.51338753369549 21.85325703472143 18-19 21.27765206621132 27.23402520005842 30.798849059502558 20.689473674227695 20-21 22.589271463843076 26.369181681904237 30.459598246100413 20.581948608152278 22-23 21.822261464907953 26.542862475652186 30.394495715282137 21.24038034415772 24-25 21.357318857821173 26.770833358043806 30.772553681223545 21.09929410291148 26-27 21.346769260762315 26.930120006441584 30.61002661776505 21.113084115031057 28-29 20.999251260015317 26.990665297099408 30.821287363180634 21.18879607970464 30-31 21.38001474079815 26.794649488672633 30.67791071477694 21.14742505575228 32-33 21.095552465013206 26.92175973710573 30.390105605476457 21.592582192404603 34-35 21.62122795833162 26.930807498901643 30.286038251060994 21.161926291705743 36-37 21.818962563995203 26.643035228289207 30.164627067354356 21.37337514036123 38-39 21.360511925693054 26.94002538509889 30.268888461338143 21.430574227869908 40-41 21.472905435178337 26.916264015506318 30.355105864022924 21.255724685292417 42-43 21.497031852581618 26.990804189799693 29.893423551948885 21.618740405669808 44-45 21.485394736372644 27.145393761142305 29.752612606398227 21.616598896086828 46-47 21.646580059477227 27.33754491596261 29.19988334813683 21.815991676423334 48-49 21.79463115125571 27.306385603707035 29.043664152791827 21.85531909224543 50-51 21.78239914210156 27.661682003329872 28.76616915186187 21.789749702706697 52-53 21.871671658955204 27.782341453504156 28.626784026484703 21.71920286105594 54-55 21.54625445773297 27.62542127405118 28.732406627545572 22.095917640670272 56-57 21.676442622167343 27.994113740620826 28.513667197133813 21.815776440078018 58-59 21.683979989548767 27.46765371705876 28.783306175024403 22.065060118368066 60-61 21.694009990596832 27.549946449241318 28.99426043277378 21.76178312738807 62-63 21.62832754388711 27.456962156005314 29.00576787754191 21.908942422565662 64-65 21.45433291314524 27.410671572217915 28.96402741126629 22.17096810337055 66-67 21.43427441706655 27.61878138895299 28.712800150357694 22.234144043622763 68-69 21.53997005802804 27.502310366717776 28.774820381479376 22.182899193774812 70-71 21.54096882446079 27.638584056900385 28.694652919784875 22.125794198853953 72-73 21.660951449279846 27.54316864593836 28.53431913894425 22.261560765837544 74-75 21.439964065610713 27.892237753187082 28.290837301723254 22.37696087947895 76-77 21.504389626637934 27.73880115978431 28.39599641313629 22.360812800441458 78-79 21.560293808125742 27.76864928884592 28.39034576084152 22.280711142186817 80-81 21.480177887702443 28.025660538895096 28.30980522723489 22.184356346167565 82-83 21.63677316696675 27.987260873805113 28.087418566872085 22.288547392356048 84-85 21.56698721074326 27.72048207672603 28.111628305239144 22.600902407291564 86-87 21.596117834068423 27.613741425048122 28.23099762597428 22.559143114909173 88-89 21.671833621593358 27.469487690055967 28.225679651014673 22.632999037335995 90-91 21.519935686957837 27.64994500606801 28.35840674382868 22.471712563145477 92-93 21.535697214586808 27.488797890460702 28.385109408677557 22.590395486274932 94-95 21.425138553881485 27.434477468547904 28.439079356030778 22.70130462153984 96-97 21.67338582564764 27.30644004325322 28.257344780290044 22.762829350809096 98-99 21.56141735767826 27.30450997773632 28.088853931878553 23.045218732706868 100-101 21.761173424217382 26.871926466887313 28.569080488806236 22.797819620089065 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3280.0 1 1970.0 2 809.5 3 940.5 4 804.0 5 823.5 6 1359.0 7 2725.0 8 4234.5 9 4620.0 10 4062.5 11 3654.0 12 3726.0 13 3926.0 14 4143.5 15 4274.0 16 4478.5 17 4642.0 18 4820.5 19 5073.5 20 5340.5 21 5922.5 22 6769.5 23 8036.0 24 9310.0 25 10997.5 26 13944.0 27 17319.5 28 19965.5 29 23035.0 30 27006.0 31 31314.0 32 36493.0 33 41490.5 34 46701.5 35 50739.0 36 55128.5 37 60685.5 38 63082.0 39 63546.5 40 64204.0 41 66845.5 42 69837.5 43 72299.0 44 74785.5 45 77654.0 46 78738.0 47 78393.0 48 83595.5 49 82483.0 50 74319.5 51 69354.5 52 65720.0 53 63966.0 54 61035.5 55 55094.5 56 48410.0 57 43031.0 58 39260.5 59 34774.5 60 29766.5 61 25580.5 62 21824.5 63 16879.0 64 12094.5 65 9522.0 66 7337.0 67 5984.0 68 4649.0 69 3085.5 70 2343.0 71 1881.5 72 1609.0 73 1421.0 74 1157.0 75 992.0 76 761.5 77 479.0 78 320.0 79 224.0 80 178.5 81 142.5 82 95.0 83 66.0 84 53.5 85 41.5 86 27.5 87 19.5 88 10.0 89 6.5 90 5.0 91 5.0 92 5.0 93 4.0 94 2.0 95 1.5 96 2.5 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3043657161472452 2 0.1867009985638749 3 0.18760064831602138 4 0.209050192406672 5 0.22202408883236352 6 0.11340321875741323 7 0.13059126402210675 8 0.1444174602129897 9 0.14853690907808154 10-11 0.13402413807634994 12-13 0.12360714094623267 14-15 0.13897221171315569 16-17 0.11432654350303727 18-19 0.14437011022603463 20-21 0.17057832800567063 22-23 0.17446102693598708 24-25 0.19837277034830178 26-27 0.17777552602284258 28-29 0.1763550264141902 30-31 0.16567760435582 32-33 0.1756211016163865 34-35 0.3622984251867839 36-37 0.48358541677221767 38-39 0.4507008508319156 40-41 0.44066265359743895 42-43 0.4630828724206687 44-45 0.4671312963053279 46-47 0.47122707017694215 48-49 0.4828514919744139 50-51 0.47598574386592757 52-53 0.4825673920526835 54-55 0.4653319968010349 56-57 0.4294407066890853 58-59 0.41819508478725415 60-61 0.3719104725386648 62-63 0.4791818679853953 64-65 0.47806914329195105 66-67 0.48666316592429776 68-69 0.498832112571753 70-71 0.4908062897828672 72-73 0.48457976649827433 74-75 0.48888861531118644 76-77 0.4648821719249617 78-79 0.4517425505449273 80-81 0.4490199262950103 82-83 0.4615676728381061 84-85 0.46298817244675844 86-87 0.46438499706193337 88-89 0.4712980951573748 90-91 0.489480490148125 92-93 0.49393138892190236 94-95 0.49262926428063775 96-97 0.49033278991331636 98-99 0.4878942655851298 100-101 0.4694277706726492 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2111933.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.825755324471665 #Duplication Level Percentage of deduplicated Percentage of total 1 70.78049580534845 33.851306741313884 2 14.68630283759673 14.047670522639901 3 5.950970902019169 8.538290349090577 4 2.8752645252499724 5.500467907109535 5 1.586293687754799 3.7932846891657435 6 0.9902217059730652 2.841486061610925 7 0.6294468456007607 2.107263957922373 8 0.4597331751694479 1.7589669080157189 9 0.31695609196861607 1.3642798052782594 >10 1.523463971198536 13.06079697704026 >50 0.12467852034047913 4.112882412146768 >100 0.0718976277585145 6.173901221235061 >500 0.0031808774112520527 1.0348973401759352 >1k 7.9521935281301E-4 0.5358704037145989 >5k 1.988048382032525E-4 0.7045421737979379 >10k+ 9.940241910162625E-5 0.5740925297425032 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12076 0.571798442469529 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8500 0.40247488911816803 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6320 0.2992519175560967 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 4.7349986955078594E-5 0.0 18-19 0.0 0.0 0.0 4.7349986955078594E-5 0.0 20-21 0.0 0.0 0.0 4.7349986955078594E-5 0.0 22-23 0.0 0.0 0.0 4.4982487607324664E-4 0.0 24-25 0.0 0.0 0.0 0.0010653747064892684 0.0 26-27 0.0 0.0 0.0 0.001988699452113301 0.0 28-29 0.0 0.0 0.0 0.005539948473744195 0.0 30-31 0.0 0.0 0.0 0.013636796243062636 0.0 32-33 0.0 0.0 0.0 0.02935699191214873 0.0 34-35 0.0 0.0 0.0 0.05426308505052007 0.0 36-37 0.0 0.0 0.0 0.09401339909930856 0.0 38-39 0.0 0.0 0.0 0.15509488227135992 0.0 40-41 0.0 0.0 0.0 0.22076931417805395 0.0 42-43 0.0 0.0 0.0 0.2893084202955302 0.0 44-45 0.0 0.0 0.0 0.3776161459667518 0.0 46-47 0.0 0.0 0.0 0.4827567920005038 0.0 48-49 0.0 0.0 0.0 0.5964914606666025 0.0 50-51 0.0 0.0 0.0 0.7219926010910384 0.0 52-53 0.0 0.0 0.0 0.846167941880732 0.0 54-55 0.0 0.0 0.0 0.987128853046001 0.0 56-57 0.0 0.0 0.0 1.154605756906114 0.0 58-59 0.0 0.0 0.0 1.3441240796938159 0.0 60-61 0.0 0.0 0.0 1.5563940712134334 0.0 62-63 0.0 0.0 0.0 1.7706054121982089 0.0 64-65 0.0 0.0 0.0 2.0010814737020537 0.0 66-67 0.0 0.0 0.0 2.2614827269615088 0.0 68-69 0.0 0.0 0.0 2.5580120202676886 0.0 70-71 0.0 0.0 0.0 2.8883018542728394 0.0 72-73 0.0 0.0 0.0 3.2356140085883407 0.0 74-75 0.0 0.0 0.0 3.597794058807737 0.0 76-77 0.0 0.0 0.0 3.991603900313125 0.0 78-79 0.0 0.0 0.0 4.426773955423775 0.0 80-81 0.0 0.0 0.0 4.907755122913464 0.0 82-83 0.0 0.0 0.0 5.402846586515766 0.0 84-85 0.0 0.0 0.0 5.920879118797803 0.0 86-87 0.0 0.0 0.0 6.462420919603037 0.0 88-89 0.0 0.0 0.0 7.037960011042017 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13290 0.0 38.100628 1 GGTATCA 8155 0.0 34.799255 1 ATCAACG 17250 0.0 28.997053 3 TATCAAC 17445 0.0 28.917528 2 TCAACGC 17335 0.0 28.881536 4 CAACGCA 17620 0.0 28.41371 5 AACGCAG 18120 0.0 27.613937 6 GTACATG 14295 0.0 24.798712 1 ACGCAGA 20315 0.0 24.560328 7 CGCAGAG 20495 0.0 24.275843 8 TACATGG 14150 0.0 24.12496 2 CATGGGG 4125 0.0 24.021633 4 ACATGGG 14140 0.0 22.96845 3 GCAGAGT 21385 0.0 22.776442 9 AGTACTT 10340 0.0 22.680395 12-13 GAGTACT 9815 0.0 22.61441 12-13 GTACTTT 10755 0.0 20.488077 14-15 AGAGTAC 19735 0.0 20.285732 10-11 TACTTTT 12250 0.0 18.99346 14-15 ATGGGGG 2275 0.0 18.755602 5 >>END_MODULE