FastQCFastQC Report
Fri 27 May 2016
SRR939247_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939247_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2111933
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128670.6092522821509962No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97960.4638404722119499No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70730.33490645773327093No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24070.11397141860087419No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG554.906077E-543.2688641
GGTATCA87950.037.719431
GTATCAA140650.036.6147161
ATCAACG179600.028.4814763
TATCAAC182000.028.2101842
TCAACGC181400.028.17274
CAACGCA183800.027.804835
AACGCAG189500.026.9684876
GTACATG142200.024.1995261
ACGCAGA212000.024.0391167
CGCAGAG213000.023.9039768
TACATGG141500.023.6083852
GAGTACT109600.022.88312512-13
GCAGAGT224450.022.6010559
ACATGGG142100.022.5736963
AGTACTT115850.021.91485612-13
TATACCG1353.823676E-421.089565
GTACTTT119050.020.84746214-15
CATGGGC20600.019.5795084
AGAGTAC207900.019.53867310-11
Produced by FastQC (version 0.11.5)