##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939247_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2111933 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.302916333046554 33.0 31.0 34.0 31.0 34.0 2 32.497651677396966 34.0 31.0 34.0 31.0 34.0 3 32.579689791295465 34.0 31.0 34.0 31.0 34.0 4 36.1165197948988 37.0 35.0 37.0 35.0 37.0 5 36.06794439028132 37.0 35.0 37.0 35.0 37.0 6 36.10411930681513 37.0 35.0 37.0 35.0 37.0 7 36.04836943217422 37.0 35.0 37.0 35.0 37.0 8 36.02648426820358 37.0 35.0 37.0 35.0 37.0 9 37.720729776938946 39.0 37.0 39.0 35.0 39.0 10-11 37.646902624278326 39.0 37.0 39.0 35.0 39.0 12-13 37.57248691127985 39.0 37.0 39.0 35.0 39.0 14-15 38.83646948080266 40.0 38.0 41.0 35.0 41.0 16-17 38.843034556493976 40.0 38.0 41.0 35.0 41.0 18-19 38.981448038361066 40.0 38.5 41.0 35.0 41.0 20-21 38.97648149822935 40.0 38.0 41.0 35.0 41.0 22-23 38.89440881884037 40.0 38.0 41.0 35.0 41.0 24-25 38.825384375356606 40.0 38.0 41.0 35.0 41.0 26-27 38.66643686139665 40.0 38.0 41.0 34.0 41.0 28-29 38.48897289828797 40.0 38.0 41.0 34.0 41.0 30-31 38.25171395115281 40.0 38.0 41.0 34.0 41.0 32-33 38.13988890746061 40.0 37.0 41.0 33.5 41.0 34-35 38.02355993300924 40.0 37.0 41.0 33.0 41.0 36-37 37.84995570408721 40.0 37.0 41.0 33.0 41.0 38-39 37.70088918540503 40.0 37.0 41.0 33.0 41.0 40-41 37.48633716126411 40.0 37.0 41.0 33.0 41.0 42-43 37.26019220306705 40.0 36.5 41.0 32.0 41.0 44-45 36.93857664992213 40.0 36.0 41.0 31.0 41.0 46-47 36.528540914886975 40.0 36.0 41.0 30.5 41.0 48-49 36.132034491624495 39.5 35.5 41.0 29.5 41.0 50-51 35.856278821345185 39.0 35.0 41.0 28.5 41.0 52-53 35.59672513285223 39.0 35.0 40.5 28.0 41.0 54-55 35.34306486048563 39.0 35.0 40.0 27.0 41.0 56-57 35.043299905820874 38.0 35.0 40.0 26.5 41.0 58-59 34.73966835122137 38.0 34.0 40.0 26.0 41.0 60-61 34.411674044583805 38.0 34.0 40.0 26.0 41.0 62-63 34.07215096312241 37.0 34.0 40.0 25.0 41.0 64-65 33.782673503373445 37.0 33.5 40.0 24.0 41.0 66-67 33.38358674257185 36.0 33.0 39.0 23.0 41.0 68-69 32.93184419202693 36.0 32.5 39.0 22.0 40.0 70-71 32.46637606401339 35.0 32.0 38.5 20.5 40.0 72-73 31.98821553524662 35.0 31.5 37.5 19.5 39.5 74-75 31.464674778982097 35.0 31.0 37.0 18.0 39.0 76-77 29.51443795802234 33.0 28.5 35.0 11.0 37.5 78-79 30.461687705055038 34.0 30.5 36.0 11.0 38.0 80-81 30.461222491433205 34.0 31.0 36.0 9.5 37.0 82-83 30.110053207180343 34.0 30.0 35.0 7.0 37.0 84-85 29.667673879805847 34.0 30.0 35.0 2.0 36.5 86-87 29.076298348479803 34.0 29.0 35.0 2.0 36.0 88-89 28.49132193114081 34.0 29.0 35.0 2.0 35.5 90-91 28.007431343702663 33.0 27.5 35.0 2.0 35.0 92-93 27.649034320691044 33.0 27.0 35.0 2.0 35.0 94-95 27.131269079085367 33.0 26.5 35.0 2.0 35.0 96-97 26.41248112511145 33.0 25.0 34.5 2.0 35.0 98-99 25.43279332251544 32.0 22.0 34.0 2.0 35.0 100-101 21.163044708331185 27.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22466311883669476 1101 2 0.3299120004647733 1101 3 0.2362865886797607 1101 4 0.10184248592339173 1101 5 0.18681404781048627 1101 6 0.21625805343248317 1101 7 0.152126741590223 1101 8 0.22038410846123924 1101 9 0.2378887975426025 1101 10-11 0.30659150345336883 1101 12-13 0.2681532894961478 1101 14-15 0.39303776846072225 1101 16-17 0.3782286696247823 1101 18-19 0.3876195812901031 1101 20-21 0.37571564298005455 1101 22-23 0.3668201393770758 1101 24-25 0.31357273463894586 1101 26-27 0.21145531657299443 1101 28-29 0.28053080383496365 1101 30-31 0.347745625933193 1101 32-33 0.41347027656576074 1101 34-35 0.4233879830366547 1101 36-37 0.4633552313616178 1101 38-39 0.5726626461636641 1101 40-41 0.5337722878353475 1101 42-43 0.4864122665105697 1101 44-45 0.5223474625447082 1101 46-47 0.5373215680481138 1101 48-49 0.4835712566625787 1101 50-51 0.4440102890962905 1101 52-53 0.5157598325574426 1101 54-55 0.42674885255777184 1101 56-57 0.518767428390575 1101 58-59 0.5395094343488935 1101 60-61 0.5724263261676867 1101 62-63 0.4739607590032122 1101 64-65 0.5412055716188533 1101 66-67 0.4694074013153866 1101 68-69 0.6765509782470573 1101 70-71 0.5270651819098049 1101 72-73 0.5472000602514697 1101 74-75 0.6504326211812703 1101 76-77 0.6821304098370895 1101 78-79 0.7775693676142659 1101 80-81 0.7761811896707869 1101 82-83 0.7708798646326542 1101 84-85 0.5666077715695046 1101 86-87 0.6026390177224776 1101 88-89 0.7638509346279712 1101 90-91 0.9602448047000571 1101 92-93 1.0585433666100066 1101 94-95 0.9289098050526157 1101 96-97 1.0231029971947017 1101 98-99 0.9463249315250302 1101 100-101 1.106834571594728 1102 1 0.15791717402734662 1102 2 0.19821708208006328 1102 3 0.25510136623921653 1102 4 0.11246547289080411 1102 5 0.12589348365698783 1102 6 0.16196275513781444 1102 7 0.16384711990893663 1102 8 0.2252138238413366 1102 9 0.25242852256963033 1102 10-11 0.24721998874358775 1102 12-13 0.2625462789214694 1102 14-15 0.34708059854833806 1102 16-17 0.3273339490091942 1102 18-19 0.2692335876636136 1102 20-21 0.3622744459085112 1102 22-23 0.32679717997230995 1102 24-25 0.31764163558531067 1102 26-27 0.3327147876826473 1102 28-29 0.3159245164247011 1102 30-31 0.28886469800952597 1102 32-33 0.273721522923303 1102 34-35 0.4018041535045569 1102 36-37 0.5442193631167811 1102 38-39 0.5147358974625931 1102 40-41 0.5012256266812116 1102 42-43 0.5006215238675935 1102 44-45 0.3986873412974532 1102 46-47 0.5297307569247351 1102 48-49 0.5588492629451594 1102 50-51 0.4455832072281112 1102 52-53 0.4151128206188375 1102 54-55 0.43819829285124 1102 56-57 0.4126605961206806 1102 58-59 0.40018108218345105 1102 60-61 0.29396312870117924 1102 62-63 0.3950716191142334 1102 64-65 0.25034685294117054 1102 66-67 0.2907643362348509 1102 68-69 0.17889394572170403 1102 70-71 0.16575604119678644 1102 72-73 0.30782610184265735 1102 74-75 0.3914948630478001 1102 76-77 0.5042966069441484 1102 78-79 0.4372695030122422 1102 80-81 0.5478764774518652 1102 82-83 0.5387675283569386 1102 84-85 0.4318471475169652 1102 86-87 0.3291413598374966 1102 88-89 0.4732461049125867 1102 90-91 0.4983748918918032 1102 92-93 0.45773723008394995 1102 94-95 0.627441312431305 1102 96-97 0.32738018085999343 1102 98-99 -0.09726881203717497 1102 100-101 -0.12969816047498384 1103 1 0.05426508995218171 1103 2 0.1431992696861144 1103 3 0.10216052411671228 1103 4 0.044885367270246945 1103 5 0.06693036392192653 1103 6 -0.023246856032088203 1103 7 0.16410707554094017 1103 8 0.1315829875866399 1103 9 0.14153445908696227 1103 10-11 0.10642517648838634 1103 12-13 0.17530901382189512 1103 14-15 0.26179592623453374 1103 16-17 0.1485680612063831 1103 18-19 0.1615634755399853 1103 20-21 0.18509211419844007 1103 22-23 0.2498532384431016 1103 24-25 0.153253133147075 1103 26-27 0.2057009674047663 1103 28-29 0.18179869455532582 1103 30-31 0.1118215386876571 1103 32-33 0.2524766280005437 1103 34-35 0.187823472136607 1103 36-37 0.2933751342153954 1103 38-39 0.24206835146047467 1103 40-41 0.27410840925967506 1103 42-43 0.4036543117120317 1103 44-45 0.5193284871751942 1103 46-47 0.4763813414487714 1103 48-49 0.47631418565223527 1103 50-51 0.563533268998917 1103 52-53 0.5132606994310223 1103 54-55 0.5570874547910662 1103 56-57 0.6464016669372725 1103 58-59 0.48266099204320767 1103 60-61 0.4831656918135536 1103 62-63 0.5674756988294973 1103 64-65 0.6118139054455227 1103 66-67 0.38109891835826915 1103 68-69 0.38571080154579107 1103 70-71 0.4903516054008108 1103 72-73 0.5706717550668614 1103 74-75 0.43456474681169155 1103 76-77 0.6050900149374066 1103 78-79 0.5297585405745728 1103 80-81 0.7487816412907833 1103 82-83 0.8374043076487574 1103 84-85 0.6598863297684368 1103 86-87 0.6254778243405994 1103 88-89 0.9070915759170894 1103 90-91 0.9466157194701701 1103 92-93 0.6147358040893884 1103 94-95 0.3450571932964017 1103 96-97 0.4257253250392452 1103 98-99 0.10278292832817115 1103 100-101 0.08168212870267055 1104 1 0.12811148961362306 1104 2 0.10566825834389704 1104 3 0.12140860837687484 1104 4 0.11175434988230393 1104 5 0.08492773834893086 1104 6 0.06475572203876823 1104 7 0.07952681095400749 1104 8 0.15559586066738973 1104 9 0.1904794796748206 1104 10-11 0.07931116918601333 1104 12-13 0.10953662897680516 1104 14-15 0.16840509371629508 1104 16-17 0.10595216137544838 1104 18-19 0.09806309660243784 1104 20-21 0.10797778301781591 1104 22-23 0.14345734478744987 1104 24-25 0.1195689856917852 1104 26-27 0.2773452648804593 1104 28-29 0.16075524520880435 1104 30-31 0.16410763329896128 1104 32-33 0.17613852502021388 1104 34-35 0.22191138780734576 1104 36-37 0.2226776408171105 1104 38-39 0.2428011259958751 1104 40-41 0.30900817973795114 1104 42-43 0.3940907268548486 1104 44-45 0.25519066186648587 1104 46-47 0.37599338928774273 1104 48-49 0.41528715693287666 1104 50-51 0.3703882060909649 1104 52-53 0.417502047023639 1104 54-55 0.3630873533092114 1104 56-57 0.28294144890243444 1104 58-59 0.23582393303043858 1104 60-61 0.3839069951428087 1104 62-63 0.582260740836503 1104 64-65 0.500077529384015 1104 66-67 0.5851961941103738 1104 68-69 0.4778263634637554 1104 70-71 0.41927166577170993 1104 72-73 0.30186711410748757 1104 74-75 0.2846007512768942 1104 76-77 0.3278507747125907 1104 78-79 0.46253518117983816 1104 80-81 0.5724801365524428 1104 82-83 0.6344190138803896 1104 84-85 0.46330033037732576 1104 86-87 0.40258589387510213 1104 88-89 0.646590363532848 1104 90-91 0.45584570973223393 1104 92-93 0.4006458048019219 1104 94-95 0.42303439010857247 1104 96-97 0.3481262560812475 1104 98-99 0.45539727354351456 1104 100-101 0.6057147043768225 1105 1 0.16087583750796597 1105 2 0.13550642492035792 1105 3 0.1711898419797251 1105 4 0.05068567913089339 1105 5 0.11724381137848638 1105 6 0.15152892800970363 1105 7 0.1268229078640104 1105 8 0.13793445072932542 1105 9 0.1963597303782194 1105 10-11 0.15724798928525274 1105 12-13 0.23008641640775096 1105 14-15 0.21195799221612077 1105 16-17 0.16018825555861582 1105 18-19 0.15661977548476358 1105 20-21 0.20304975489011667 1105 22-23 0.1978204846120164 1105 24-25 0.21026872829524734 1105 26-27 0.17431073325539614 1105 28-29 0.2873081974189873 1105 30-31 0.26832999002659363 1105 32-33 0.1939868883005076 1105 34-35 0.21238038247349067 1105 36-37 0.19312351937387717 1105 38-39 0.34084317707028333 1105 40-41 0.21213536172101755 1105 42-43 0.21196976700953485 1105 44-45 0.2189557988898585 1105 46-47 0.2252026076368594 1105 48-49 0.34911910137158486 1105 50-51 0.30939751863976994 1105 52-53 0.38214514173152025 1105 54-55 0.29744865458710734 1105 56-57 0.33670912683863463 1105 58-59 0.24843915206538014 1105 60-61 0.2806395465084961 1105 62-63 0.2964354192974099 1105 64-65 0.1330612634103474 1105 66-67 0.21157097255515822 1105 68-69 0.2906287424192229 1105 70-71 0.34828310607679214 1105 72-73 0.39453554770702937 1105 74-75 0.30751802907096604 1105 76-77 0.4467887351239277 1105 78-79 0.4841282922074015 1105 80-81 0.44882496114682624 1105 82-83 0.3563470915868727 1105 84-85 0.4498143838976283 1105 86-87 0.5274588482336959 1105 88-89 0.7686714124547152 1105 90-91 0.7212776235973557 1105 92-93 0.7496969907678199 1105 94-95 0.7864267566280212 1105 96-97 0.736612202560945 1105 98-99 0.6263676543499166 1105 100-101 0.4232219036298339 1106 1 0.0996813981445186 1106 2 0.09206494063380433 1106 3 0.07170299460555185 1106 4 0.1017028385987615 1106 5 0.11603656791772465 1106 6 0.1383177197280716 1106 7 0.10016739740568426 1106 8 0.05306948699112013 1106 9 0.1628876447934573 1106 10-11 0.12212002246714349 1106 12-13 0.10135246478647986 1106 14-15 0.19268930886293134 1106 16-17 0.11444673023962793 1106 18-19 0.16441111813525566 1106 20-21 0.22675871627514255 1106 22-23 0.1867673105306764 1106 24-25 0.2445144977522915 1106 26-27 0.20606835515386024 1106 28-29 0.24030183491966284 1106 30-31 0.19368234988599653 1106 32-33 0.24170340855224737 1106 34-35 0.3020594890540096 1106 36-37 0.35371495496531224 1106 38-39 0.34245666635982985 1106 40-41 0.4873652422325918 1106 42-43 0.3589873763510951 1106 44-45 0.4438142864244057 1106 46-47 0.4171952267421517 1106 48-49 0.27513774393951707 1106 50-51 0.40730214541763843 1106 52-53 0.4487159424572482 1106 54-55 0.453884567361051 1106 56-57 0.5631495877861354 1106 58-59 0.6194035131705888 1106 60-61 0.5645369110396672 1106 62-63 0.5774486485500816 1106 64-65 0.4516909778147138 1106 66-67 0.49154479583884125 1106 68-69 0.553066514011924 1106 70-71 0.322098479892162 1106 72-73 0.46016025417680595 1106 74-75 0.373067344620285 1106 76-77 0.3402651958539131 1106 78-79 0.525485526522381 1106 80-81 0.48759801717340423 1106 82-83 0.6778194144676455 1106 84-85 0.558190067977538 1106 86-87 0.5877336419591934 1106 88-89 0.90311554654674 1106 90-91 0.9903486838655944 1106 92-93 1.0579492067665157 1106 94-95 1.3031988881888843 1106 96-97 1.0340718993164195 1106 98-99 1.0962369932333367 1106 100-101 1.1757795097085726 1107 1 0.003267640799201388 1107 2 0.061819053543807456 1107 3 0.035269758742657586 1107 4 0.009721261446081542 1107 5 0.0861886591724712 1107 6 0.11455135917464077 1107 7 0.05419225703890618 1107 8 0.06451580195454198 1107 9 0.15744276853109085 1107 10-11 0.12791371366957804 1107 12-13 0.08099414414298423 1107 14-15 0.17861152558362647 1107 16-17 0.22011722098616104 1107 18-19 0.20671406747243282 1107 20-21 0.21537417670619163 1107 22-23 0.17154113551362826 1107 24-25 0.2729260409927292 1107 26-27 0.2407971039510528 1107 28-29 0.3516251829825592 1107 30-31 0.311259142890556 1107 32-33 0.2941907732069069 1107 34-35 0.3254542630191324 1107 36-37 0.36880928797789636 1107 38-39 0.31558612142844567 1107 40-41 0.38102608247137937 1107 42-43 0.46468472596478705 1107 44-45 0.38554420766953257 1107 46-47 0.290531845420972 1107 48-49 0.20981464344531986 1107 50-51 0.321708073987061 1107 52-53 0.2709102418378606 1107 54-55 0.2069752055277334 1107 56-57 0.28936804613388034 1107 58-59 0.2405094445426741 1107 60-61 0.3549843797666057 1107 62-63 0.3495559485566204 1107 64-65 0.2558995287437966 1107 66-67 0.4107470174174992 1107 68-69 0.3980589701735795 1107 70-71 0.3276421500746167 1107 72-73 0.3152829408109028 1107 74-75 0.2501103701899261 1107 76-77 0.268107331110496 1107 78-79 0.41551317947779154 1107 80-81 0.3821411128096557 1107 82-83 0.40055626296495106 1107 84-85 0.367050691476944 1107 86-87 0.5067279992615354 1107 88-89 0.6601686850637556 1107 90-91 0.6724791292089698 1107 92-93 0.28185476631846385 1107 94-95 0.46989926468013365 1107 96-97 0.4945184805329603 1107 98-99 0.27638945817667704 1107 100-101 0.44490045401962774 1108 1 0.11370689139258872 1108 2 0.06243355922083538 1108 3 0.08595701978044445 1108 4 0.06821938669791194 1108 5 0.1446344392735739 1108 6 0.05831881122384175 1108 7 0.024080819051633284 1108 8 -0.014321053606266787 1108 9 0.012471114090025992 1108 10-11 -0.010266817277397422 1108 12-13 0.029273836159951827 1108 14-15 0.09434463041645103 1108 16-17 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-0.705333846856746 2316 80-81 -0.6542826979147129 2316 82-83 -0.5360446528659644 2316 84-85 -0.46424689200761193 2316 86-87 -0.7127683670239264 2316 88-89 -0.944009646916161 2316 90-91 -1.056207060916826 2316 92-93 -1.0559821108270988 2316 94-95 -0.7095126422944276 2316 96-97 -0.7168091738082936 2316 98-99 -0.4164187479497734 2316 100-101 0.07979899144586255 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 23.0 5 61.0 6 139.0 7 325.0 8 684.0 9 1284.0 10 2287.0 11 3551.0 12 5490.0 13 7416.0 14 9257.0 15 12877.0 16 17412.0 17 25232.0 18 23337.0 19 14005.0 20 12117.0 21 12718.0 22 13334.0 23 14366.0 24 16347.0 25 19335.0 26 22937.0 27 27727.0 28 33521.0 29 40773.0 30 49570.0 31 60396.0 32 75161.0 33 97436.0 34 133143.0 35 192625.0 36 297564.0 37 473697.0 38 372380.0 39 23398.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.6916804314561 20.174305451996425 15.384301206203988 21.74971291034349 2 15.371794465070627 21.47478163369766 40.342330935687826 22.81109296554389 3 18.873562750333463 26.295152355685524 30.58023147514623 24.251053418834783 4 12.678337807117934 17.584222605546675 37.548113505494726 32.18932608184066 5 13.075935647579728 38.6084217633798 33.5816050982678 14.734037490772673 6 30.15090914342453 37.18801685470135 17.862024979012116 14.799049022861993 7 27.47890202956249 34.40525812135139 20.63209391585813 17.483745933227997 8 26.204003630797 35.06758973887903 19.886094871380863 18.84231175894311 9 27.18107061161505 16.97250812407401 20.706480745364555 35.13994051894639 10-11 24.679641825758676 27.567683255103265 26.887453342506607 20.865221576631455 12-13 25.97762334316477 25.160646668241842 27.79401619274854 21.06771379584485 14-15 22.85332843413234 25.998042529294587 27.043707849154487 24.104921187418586 16-17 21.162971564940392 29.510338353652543 27.520606273682564 21.806083807724498 18-19 21.16494837306436 27.20574848462106 31.016693020911433 20.612610121403144 20-21 22.486414104992914 26.32304149800207 30.750857153138856 20.439687243866164 22-23 21.736508686591858 26.459859285308767 30.645219332242075 21.1584126958573 24-25 21.32191220081319 26.693933945821197 31.02854115163691 20.9556127017287 26-27 21.33860465578768 26.950697477970987 30.856436800358722 20.85426106588261 28-29 20.980068969991 26.94344470208098 31.08098126218966 20.995505065738353 30-31 21.230739800931183 26.751203755043367 30.9901876622033 21.02786878182215 32-33 21.090086664681124 26.873532446341812 30.68300462183223 21.35337626714484 34-35 21.507104132786306 26.86272035501605 30.595843704106883 21.03433180809075 36-37 21.73144165184499 26.593235443977854 30.470429572889497 21.204893331287657 38-39 21.31933497706058 26.881261935529082 30.541861774439393 21.25754131297094 40-41 21.4019073721085 26.82743659198758 30.616871348151403 21.153784687752516 42-43 21.494685664148747 26.913972960265237 30.14199340981633 21.449347965769682 44-45 21.3872139900431 27.124009542109434 29.943884706771435 21.544891761076034 46-47 21.63895110159901 27.257579559928597 29.368373951788172 21.73509538668422 48-49 21.790078196450676 27.257508495213727 29.17658751532074 21.775825793014857 50-51 21.798471305309235 27.660093303171386 28.826962982955184 21.714472408564195 52-53 21.909522612244434 27.798635466783715 28.625580097077037 21.666261823894814 54-55 21.54507268933247 27.61553041692137 28.772764098103494 22.066632795642665 56-57 21.70652147149792 27.95712518869122 28.473386252104728 21.86296708770614 58-59 21.720437031993264 27.425253873085715 28.801419776294317 22.052889318626704 60-61 21.72728975188638 27.632852734875048 28.86064807043653 21.779209442802046 62-63 21.59534580125617 27.489755075939932 28.987671998596525 21.92722712420738 64-65 21.407649488714597 27.422024004560754 28.928133102770353 22.242193403954293 66-67 21.441068484407126 27.596042664373655 28.685545800373685 22.277343050845534 68-69 21.461059108163056 27.49389826551879 28.791213786451486 22.25382883986667 70-71 21.494337178310108 27.622561889984198 28.6439484585922 22.239152473113492 72-73 21.56983673250998 27.531957689945656 28.448677112389454 22.44952846515491 74-75 21.405626977749765 27.90630668681251 28.130911349933925 22.557154985503804 76-77 21.37706382626227 27.690548713550967 28.44405402281308 22.488333437373683 78-79 21.410586859078393 27.681061323306484 28.364748917991818 22.54360289962331 80-81 21.2995796733697 28.00986680896943 28.277086966465255 22.41346655119561 82-83 21.497628002403484 27.879293519254635 28.05067679703854 22.572401681303337 84-85 21.294307158418377 27.602106695619604 28.146891970531264 22.956694175430755 86-87 21.287212248817617 27.546804744890558 28.19409381899606 22.971889187295766 88-89 21.33027909888995 27.410312556063936 28.235003380172586 23.024404964873533 90-91 21.154040633040186 27.60736429342276 28.303019109616912 22.935575963920147 92-93 21.131281153332043 27.42636721903583 28.322844522056336 23.119507105575792 94-95 20.955470651767836 27.313342800174055 28.387714004184794 23.343472543873315 96-97 21.103439361002458 27.202070330829624 28.20920455336415 23.485285754803776 98-99 20.920868807410475 27.22702154593702 28.032375133734337 23.819734512918163 100-101 20.95041637329321 26.750261727796463 28.792851268059927 23.506470630850405 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 989.0 1 698.0 2 558.0 3 716.0 4 609.5 5 619.0 6 1130.0 7 2480.5 8 3982.5 9 4407.5 10 3798.5 11 3289.0 12 3375.0 13 3579.0 14 3781.0 15 3972.0 16 4145.0 17 4287.0 18 4516.0 19 4801.5 20 5139.0 21 5770.5 22 6631.5 23 7840.5 24 9171.0 25 10984.0 26 13878.0 27 17284.0 28 20059.5 29 23031.5 30 27125.5 31 31577.5 32 36993.0 33 42020.5 34 47000.0 35 51382.0 36 55788.5 37 60874.5 38 63447.0 39 64294.5 40 64741.5 41 67043.5 42 70316.5 43 73180.0 44 75279.5 45 78084.0 46 79476.0 47 79187.0 48 84045.0 49 82987.5 50 75263.5 51 69930.0 52 65875.5 53 63744.0 54 60819.5 55 55231.0 56 48413.5 57 42841.5 58 39317.0 59 35029.0 60 29745.5 61 25225.0 62 21360.5 63 16623.5 64 12071.0 65 9462.0 66 7086.0 67 5680.0 68 4419.5 69 2830.5 70 2098.5 71 1742.0 72 1504.5 73 1373.5 74 1181.0 75 1014.0 76 757.0 77 445.0 78 292.0 79 220.0 80 166.0 81 123.0 82 77.5 83 40.0 84 25.5 85 14.0 86 6.0 87 3.5 88 2.5 89 1.5 90 1.0 91 1.0 92 1.5 93 1.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29920456756914166 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001136399686921886 16-17 7.339247978037182E-4 18-19 1.1837496738769648E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.1837496738769648E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.00134947462821974 36-37 0.0076233478997676535 38-39 0.003125099139035187 40-41 0.0035275740281533554 42-43 1.8939994782031437E-4 44-45 0.0014678495956074364 46-47 1.4204996086523576E-4 48-49 4.0247488911816803E-4 50-51 2.3674993477539297E-5 52-53 9.469997391015719E-5 54-55 0.0 56-57 8.049497782363361E-4 58-59 0.002414849334709008 60-61 8.286247717138754E-4 62-63 0.001586224562995133 64-65 4.7349986955078594E-5 66-67 3.314499086855502E-4 68-69 0.001586224562995133 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.2546719048378902 78-79 1.657249543427751E-4 80-81 1.8939994782031437E-4 82-83 0.0 84-85 0.0 86-87 0.10469082115767878 88-89 0.36698607389533666 90-91 0.2706525254352292 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.657249543427751E-4 100-101 2.840999217304715E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2111933.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.293145447303864 #Duplication Level Percentage of deduplicated Percentage of total 1 69.3193747908776 32.090118995102664 2 15.360404393076028 14.221628693961478 3 6.116920125251435 8.495144191434147 4 3.1107918609505574 5.7603336030109284 5 1.709494207109486 3.956893198552142 6 1.0609215764078135 2.9468038106897887 7 0.7052578403894443 2.285402264809997 8 0.4469110843110797 1.6551135862420074 9 0.34699344098554274 1.4457076049523763 >10 1.6080785556979738 13.357307958020288 >50 0.13408543454896588 4.300419781800844 >100 0.07616191647051211 6.389763508619499 >500 0.0033768342106244237 1.0630524563874117 >1k 9.209547847157487E-4 0.6236864952897209 >5k 2.0465661882572195E-4 0.7991012827770242 >10k+ 1.0232830941286097E-4 0.6095225683497523 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12867 0.6092522821509962 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9796 0.4638404722119499 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7073 0.33490645773327093 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2407 0.11397141860087419 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.3674993477539297E-5 0.0 14-15 0.0 0.0 0.0 4.7349986955078594E-5 0.0 16-17 0.0 0.0 0.0 9.469997391015719E-5 0.0 18-19 0.0 0.0 0.0 9.469997391015719E-5 0.0 20-21 0.0 0.0 0.0 9.469997391015719E-5 0.0 22-23 0.0 0.0 0.0 4.734998695507859E-4 0.0 24-25 0.0 0.0 0.0 0.0010653747064892684 0.0 26-27 0.0 0.0 0.0 0.001965024458635762 0.0 28-29 0.0 0.0 0.0 0.005587298460699274 0.0 30-31 0.0 0.0 0.0 0.01375517121045033 0.0 32-33 0.0 0.0 0.0 0.029570066853446583 0.0 34-35 0.0 0.0 0.0 0.054689234933115775 0.0 36-37 0.0 0.0 0.0 0.09467629891667964 0.0 38-39 0.0 0.0 0.0 0.15582880706916366 0.0 40-41 0.0 0.0 0.0 0.22204776382584107 0.0 42-43 0.0 0.0 0.0 0.29058686994331734 0.0 44-45 0.0 0.0 0.0 0.3792733955101795 0.0 46-47 0.0 0.0 0.0 0.48540839126998825 0.0 48-49 0.0 0.0 0.0 0.6003268096099639 0.0 50-51 0.0 0.0 0.0 0.7267986247669789 0.0 52-53 0.0 0.0 0.0 0.8520866902501167 0.0 54-55 0.0 0.0 0.0 0.9939235761740548 0.0 56-57 0.0 0.0 0.0 1.1628920046232527 0.0 58-59 0.0 0.0 0.0 1.353641427071787 0.0 60-61 0.0 0.0 0.0 1.5676396931152645 0.0 62-63 0.0 0.0 0.0 1.7845973333434346 0.0 64-65 0.0 0.0 0.0 2.0162808195146344 0.0 66-67 0.0 0.0 0.0 2.276587372800179 0.0 68-69 0.0 0.0 0.0 2.5729509411520155 0.0 70-71 0.0 0.0 0.0 2.902956675235436 0.0 72-73 0.0 0.0 0.0 3.2505529294726676 0.0 74-75 0.0 0.0 0.0 3.613608954450733 0.0 76-77 0.0 0.0 0.0 4.007821270845239 0.0 78-79 0.0 0.0 0.0 4.442920300975457 0.0 80-81 0.0 0.0 0.0 4.925558718008573 0.0 82-83 0.0 0.0 0.0 5.421384106408679 0.0 84-85 0.0 0.0 0.0 5.9408371382993685 0.0 86-87 0.0 0.0 0.0 6.484012513654553 0.0 88-89 0.0 0.0 0.0 7.060167154923949 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTATAG 55 4.906077E-5 43.268864 1 GGTATCA 8795 0.0 37.71943 1 GTATCAA 14065 0.0 36.614716 1 ATCAACG 17960 0.0 28.481476 3 TATCAAC 18200 0.0 28.210184 2 TCAACGC 18140 0.0 28.1727 4 CAACGCA 18380 0.0 27.80483 5 AACGCAG 18950 0.0 26.968487 6 GTACATG 14220 0.0 24.199526 1 ACGCAGA 21200 0.0 24.039116 7 CGCAGAG 21300 0.0 23.903976 8 TACATGG 14150 0.0 23.608385 2 GAGTACT 10960 0.0 22.883125 12-13 GCAGAGT 22445 0.0 22.601055 9 ACATGGG 14210 0.0 22.573696 3 AGTACTT 11585 0.0 21.914856 12-13 TATACCG 135 3.823676E-4 21.08956 5 GTACTTT 11905 0.0 20.847462 14-15 CATGGGC 2060 0.0 19.579508 4 AGAGTAC 20790 0.0 19.538673 10-11 >>END_MODULE