FastQCFastQC Report
Fri 27 May 2016
SRR939246_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939246_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2156009
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125100.5802387652370653No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88210.40913558338578365No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65410.3033846333665583No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21840.10129827843946848No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85000.036.7223741
GTATCAA134100.036.172731
ATCAACG171500.028.1553823
TATCAAC173350.027.882272
TCAACGC173500.027.8308284
CAACGCA177850.027.2301275
AACGCAG182400.026.6623176
GTACACG3050.026.4809381
GTACATG148750.024.8808271
TACATGG147700.024.5673752
CTCGTAT500.001648014323.76573846-47
ACGCAGA204600.023.6303377
CGCAGAG205650.023.4635878
ACATGGG151100.023.3867363
GAGTACT95650.023.36696612-13
GCAGAGT217050.021.9910039
AGTACTT100000.021.92387612-13
TAGCGCG1754.102081E-621.6835174
GTACTTT106800.020.92743714-15
CATGGGA98450.020.6689954
Produced by FastQC (version 0.11.5)