FastQCFastQC Report
Fri 27 May 2016
SRR939246_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939246_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2156009
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130390.6047748409213506No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100480.466046292014551No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73610.34141786977698146No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23850.1106210595595844No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90400.037.2134931
GTATCAA142900.035.3291051
TATCACG804.5213287E-429.6578312
ATCAACG180900.027.8314723
TCAACGC182350.027.584144
TATCAAC184150.027.2629782
TAGATCG700.00843420827.115735
CAACGCA185750.027.0792335
AACGCAG192000.026.2718946
ATCGGAC950.001234075524.9750163
CCCGTAG1751.4625948E-724.4711231
GTACATG139650.024.0895061
TACATGG138150.023.3913652
ACGCAGA216600.023.2004747
CGCAGAG217500.023.039028
CCGTAGG1652.4527399E-623.0072882
ACATGGG139450.022.1864813
GAGTACT108550.022.16448412-13
GCAGAGT229400.021.5552859
AGTACTT112550.021.27135512-13
Produced by FastQC (version 0.11.5)