##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939246_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2156009 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.26666818181186 33.0 31.0 34.0 31.0 34.0 2 32.45668965203763 34.0 31.0 34.0 31.0 34.0 3 32.53609191798365 34.0 31.0 34.0 31.0 34.0 4 36.087728297980206 37.0 35.0 37.0 35.0 37.0 5 36.03083567832973 37.0 35.0 37.0 35.0 37.0 6 36.07702704395019 37.0 35.0 37.0 35.0 37.0 7 36.02042106503266 37.0 35.0 37.0 35.0 37.0 8 36.0002532456961 37.0 35.0 37.0 35.0 37.0 9 37.69868214835838 39.0 37.0 39.0 35.0 39.0 10-11 37.61932603249801 39.0 37.0 39.0 35.0 39.0 12-13 37.5430684658552 39.0 37.0 39.0 35.0 39.0 14-15 38.80396464022182 40.0 38.0 41.0 34.5 41.0 16-17 38.76366100512567 40.0 38.0 41.0 34.5 41.0 18-19 38.90564557012517 40.0 38.0 41.0 35.0 41.0 20-21 38.92083196313188 40.0 38.0 41.0 35.0 41.0 22-23 38.85732457517571 40.0 38.0 41.0 35.0 41.0 24-25 38.79044521613778 40.0 38.0 41.0 34.0 41.0 26-27 38.61794152992868 40.0 38.0 41.0 34.0 41.0 28-29 38.46437027860273 40.0 38.0 41.0 34.0 41.0 30-31 38.23008252748481 40.0 37.5 41.0 34.0 41.0 32-33 37.99371616723307 40.0 37.0 41.0 33.0 41.0 34-35 37.90726708469213 40.0 37.0 41.0 33.0 41.0 36-37 37.79665599726161 40.0 37.0 41.0 33.0 41.0 38-39 37.671528968571096 40.0 37.0 41.0 33.0 41.0 40-41 37.47239969777492 40.0 37.0 41.0 33.0 41.0 42-43 37.25464202607689 40.0 36.0 41.0 32.0 41.0 44-45 36.95597142683542 40.0 36.0 41.0 31.0 41.0 46-47 36.56227038013292 40.0 36.0 41.0 30.5 41.0 48-49 36.17853404136996 39.5 35.5 41.0 29.5 41.0 50-51 35.91310704176095 39.0 35.0 41.0 28.5 41.0 52-53 35.68107252799037 39.0 35.0 41.0 28.0 41.0 54-55 35.40268431161465 39.0 35.0 40.0 27.0 41.0 56-57 35.16988148008659 38.5 35.0 40.0 27.0 41.0 58-59 34.882896360822244 38.0 34.0 40.0 26.0 41.0 60-61 34.563340412771936 38.0 34.0 40.0 26.0 41.0 62-63 34.19900566277785 37.0 34.0 40.0 25.5 41.0 64-65 33.791051892640525 37.0 33.5 40.0 24.5 41.0 66-67 33.42766194389726 36.0 33.0 39.0 23.0 41.0 68-69 33.05683394642601 36.0 33.0 39.0 22.0 40.0 70-71 32.6733269666314 35.0 32.5 38.5 22.0 40.0 72-73 32.19672668342294 35.0 32.0 38.0 20.5 40.0 74-75 31.702317569175268 35.0 31.5 37.0 18.5 39.0 76-77 29.70500911638124 33.5 28.5 35.0 13.0 37.5 78-79 30.67167344848746 34.0 30.5 36.0 13.5 38.0 80-81 30.71051164443191 34.5 31.0 36.0 13.5 37.5 82-83 30.402531946759034 34.0 31.0 35.0 8.5 37.0 84-85 30.028291393959858 34.0 30.5 35.0 6.0 36.5 86-87 29.49538429570563 34.0 30.0 35.0 2.0 36.0 88-89 28.991203886440175 34.0 29.0 35.0 2.0 36.0 90-91 28.572022658532504 34.0 29.0 35.0 2.0 35.0 92-93 28.228485363465552 34.0 29.0 35.0 2.0 35.0 94-95 27.83806306003361 34.0 28.5 35.0 2.0 35.0 96-97 27.298604736807686 33.0 27.0 35.0 2.0 35.0 98-99 26.566434555699907 33.0 25.5 35.0 2.0 35.0 100-101 23.259929805487825 29.5 11.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19787165566862797 1101 2 0.20910514317245088 1101 3 0.23043930873595286 1101 4 0.14536235969958966 1101 5 0.2256573772165993 1101 6 0.19259756144653295 1101 7 0.22398886221986203 1101 8 0.2475835883674904 1101 9 0.29122378490907863 1101 10-11 0.3150464688852921 1101 12-13 0.21984963607929586 1101 14-15 0.2641203542403048 1101 16-17 0.32517642570266503 1101 18-19 0.2561827386269968 1101 20-21 0.2230505899879418 1101 22-23 0.301634201245804 1101 24-25 0.2739914354250388 1101 26-27 0.2709881131865828 1101 28-29 0.35687130504442166 1101 30-31 0.3565062376194277 1101 32-33 0.35457116486289664 1101 34-35 0.33895936767130763 1101 36-37 0.3107157176144497 1101 38-39 0.3017270894434958 1101 40-41 0.37973088844331215 1101 42-43 0.4197801242335899 1101 44-45 0.5873684949318019 1101 46-47 0.3649326920297753 1101 48-49 0.2838058577881313 1101 50-51 0.3205711485405658 1101 52-53 0.20701226663623373 1101 54-55 0.20018300526568567 1101 56-57 0.08540576322086935 1101 58-59 0.036705014544239134 1101 60-61 0.03517170583750584 1101 62-63 0.11966140536397774 1101 64-65 0.2408261101645337 1101 66-67 0.18356461010813518 1101 68-69 0.12189545251121103 1101 70-71 0.16831998471981535 1101 72-73 0.018527628145548647 1101 74-75 0.09830290006341968 1101 76-77 0.28740061989580923 1101 78-79 0.39996635800588365 1101 80-81 0.427188913426761 1101 82-83 0.2898351974443827 1101 84-85 0.3274023742239791 1101 86-87 0.3673806847791212 1101 88-89 0.3887927379859839 1101 90-91 0.49704716602695953 1101 92-93 0.568571850220426 1101 94-95 0.6706837586206582 1101 96-97 0.5098599325958588 1101 98-99 0.44607507228512944 1101 100-101 0.3167835134373185 1102 1 0.2503031306786454 1102 2 0.25686036553381086 1102 3 0.22657938467467176 1102 4 0.09474410837284353 1102 5 0.14968758809308724 1102 6 0.15271923807794963 1102 7 0.17712723411464282 1102 8 0.22302318383132302 1102 9 0.2716646527369093 1102 10-11 0.2897591791717886 1102 12-13 0.27323265459702384 1102 14-15 0.3503001296155617 1102 16-17 0.30861805694890165 1102 18-19 0.2364048270340433 1102 20-21 0.35794678944086655 1102 22-23 0.3258187754909798 1102 24-25 0.3331315861098858 1102 26-27 0.39874059765576675 1102 28-29 0.4623901734047138 1102 30-31 0.4467025680040635 1102 32-33 0.4286072126567504 1102 34-35 0.557634647780354 1102 36-37 0.6092471660014098 1102 38-39 0.6101749985253164 1102 40-41 0.6493557384261308 1102 42-43 0.6538921611329869 1102 44-45 0.7410226151655763 1102 46-47 0.6883033732358257 1102 48-49 0.4962520132752104 1102 50-51 0.5415522209712549 1102 52-53 0.5673159375113599 1102 54-55 0.48835623489505053 1102 56-57 0.4434624308701558 1102 58-59 0.2972045370927958 1102 60-61 0.359033695915592 1102 62-63 0.4667782621293881 1102 64-65 0.3745158733872458 1102 66-67 0.33367626739794787 1102 68-69 0.3760663288970534 1102 70-71 0.3294802127207248 1102 72-73 0.49728838759988747 1102 74-75 0.4127737520098016 1102 76-77 0.6083542294203141 1102 78-79 0.5767409698508743 1102 80-81 0.49078023850778507 1102 82-83 0.4794480880454337 1102 84-85 0.47647640397294566 1102 86-87 0.6457781276214263 1102 88-89 0.742742147449519 1102 90-91 0.7965318376100718 1102 92-93 0.6311427645672296 1102 94-95 0.44957167049076574 1102 96-97 0.42779875330339223 1102 98-99 0.6012904629344717 1102 100-101 0.19422756511461614 1103 1 0.17961617104170813 1103 2 0.2030679805154776 1103 3 0.19360660981074318 1103 4 0.10270479033022895 1103 5 0.1602121313279099 1103 6 0.1330841549464452 1103 7 0.19353891792599853 1103 8 0.10682562288390329 1103 9 0.21982942003307215 1103 10-11 0.22324695501800562 1103 12-13 0.24631087300234356 1103 14-15 0.2522217048466473 1103 16-17 0.22956429180388227 1103 18-19 0.2239994860140868 1103 20-21 0.20395910191826516 1103 22-23 0.20129691993558652 1103 24-25 0.18194260163338782 1103 26-27 0.19329903734890053 1103 28-29 0.207531961702081 1103 30-31 0.2922006224081173 1103 32-33 0.2906391181191097 1103 34-35 0.3851580205208833 1103 36-37 0.44048797250334815 1103 38-39 0.35171326576451634 1103 40-41 0.407222318585184 1103 42-43 0.47352790462799277 1103 44-45 0.6852660614705925 1103 46-47 0.7115824246896949 1103 48-49 0.7773819612550739 1103 50-51 0.720518157771167 1103 52-53 0.5672606320684821 1103 54-55 0.582682556818952 1103 56-57 0.6653169131699386 1103 58-59 0.48239852712460873 1103 60-61 0.5242933756062769 1103 62-63 0.5749821229445757 1103 64-65 0.7449802079802268 1103 66-67 0.6224618919449796 1103 68-69 0.5135556598993105 1103 70-71 0.631662961714909 1103 72-73 0.7657661837027447 1103 74-75 0.8327339816181869 1103 76-77 0.8994115043973245 1103 78-79 0.9058566440737508 1103 80-81 0.8236154101280029 1103 82-83 0.8466122560617215 1103 84-85 0.7489159683311435 1103 86-87 0.6551024602002933 1103 88-89 0.8533753204796142 1103 90-91 1.0767711203232402 1103 92-93 0.940123130309555 1103 94-95 0.8547760777567426 1103 96-97 0.717633530356423 1103 98-99 0.6404401128592738 1103 100-101 0.5934953045417828 1104 1 0.04422164485593072 1104 2 0.020875219011941226 1104 3 0.03348929566389103 1104 4 0.05495854588451721 1104 5 -0.021015701754805605 1104 6 0.00792157111663272 1104 7 0.0963904532460802 1104 8 0.08944792293487325 1104 9 0.10482475665297386 1104 10-11 0.072464596341689 1104 12-13 0.14448501693900084 1104 14-15 0.15447101908656435 1104 16-17 0.07852338518356561 1104 18-19 0.0942924858681522 1104 20-21 0.13908180908972412 1104 22-23 0.06693658552576665 1104 24-25 -0.00786701402373069 1104 26-27 0.1045425419266266 1104 28-29 0.05645536594840195 1104 30-31 -0.018386307861135265 1104 32-33 -0.028119566236675553 1104 34-35 0.05361839659445877 1104 36-37 -0.05120280415565048 1104 38-39 0.019910599706939536 1104 40-41 0.051062479910640945 1104 42-43 0.12343521862680262 1104 44-45 0.06279126592967543 1104 46-47 0.05920172156618264 1104 48-49 0.0169271600884926 1104 50-51 -0.19757898223387116 1104 52-53 -0.31326140213810305 1104 54-55 -0.2808577946370576 1104 56-57 -0.14833135165083178 1104 58-59 -0.1253744603783815 1104 60-61 -0.07782539571467595 1104 62-63 -0.20074551239390104 1104 64-65 -0.20989973906238646 1104 66-67 -0.21714902776663791 1104 68-69 -0.07477388774224636 1104 70-71 -0.15447056745924215 1104 72-73 -0.20796000503619183 1104 74-75 -0.33986421901501274 1104 76-77 -0.12459036306962545 1104 78-79 -0.03271627702100943 1104 80-81 -0.055140937250456545 1104 82-83 -0.0028723928523781694 1104 84-85 -0.020993016447839352 1104 86-87 -0.05580789292649868 1104 88-89 0.16657786767915184 1104 90-91 0.30792990154198563 1104 92-93 0.30176402677828307 1104 94-95 0.2783241380655639 1104 96-97 0.3777579823299533 1104 98-99 0.3874538576698079 1104 100-101 0.19243509935305525 1105 1 0.06628426677677624 1105 2 0.08494791477833274 1105 3 0.09129652595989768 1105 4 0.06990871369669804 1105 5 0.12024880727309295 1105 6 0.0878377110579791 1105 7 0.10159922878488459 1105 8 0.16620550107928977 1105 9 0.2156329658959919 1105 10-11 0.14530574710054367 1105 12-13 0.13254777081821345 1105 14-15 0.2414448862136851 1105 16-17 0.2574611151517274 1105 18-19 0.1608746923553852 1105 20-21 0.16497135174655142 1105 22-23 0.09165960080531477 1105 24-25 0.25628245287466456 1105 26-27 0.23760824185327323 1105 28-29 0.2780012020479177 1105 30-31 0.24930327489466464 1105 32-33 0.13079833928726003 1105 34-35 0.3325350541399743 1105 36-37 0.27403175441290273 1105 38-39 0.32725546422457086 1105 40-41 0.4142491365662053 1105 42-43 0.39304292888303394 1105 44-45 0.4214852002430476 1105 46-47 0.3257022724156755 1105 48-49 0.37213519400076933 1105 50-51 0.37890483342948755 1105 52-53 0.49401070626007737 1105 54-55 0.348456681605839 1105 56-57 0.3975615206533547 1105 58-59 0.4428547963277154 1105 60-61 0.45593996490505617 1105 62-63 0.49016769582106434 1105 64-65 0.5349234520593669 1105 66-67 0.40459689405467714 1105 68-69 0.4783492038481185 1105 70-71 0.40515940182207544 1105 72-73 0.3054594712015799 1105 74-75 0.4106386804889688 1105 76-77 0.4344232889960331 1105 78-79 0.46130723612009916 1105 80-81 0.3949028442878486 1105 82-83 0.44718046721752813 1105 84-85 0.40394146468467085 1105 86-87 0.40647950320742865 1105 88-89 0.5712430565484148 1105 90-91 0.6538777053556792 1105 92-93 0.5982898389616231 1105 94-95 0.6328781341027216 1105 96-97 0.5587671519026003 1105 98-99 0.6266910666581147 1105 100-101 0.5478048845677854 1106 1 0.12616218300828308 1106 2 0.15305067348882062 1106 3 0.16829114991584504 1106 4 0.08572592288407321 1106 5 0.1520665904720886 1106 6 0.12177614831133354 1106 7 0.10675204609239586 1106 8 0.11709296025404115 1106 9 0.2682081786922055 1106 10-11 0.2172737831569833 1106 12-13 0.17644105780542674 1106 14-15 0.19040302854199354 1106 16-17 0.2158040817819824 1106 18-19 0.10295931123558688 1106 20-21 0.14920596305298517 1106 22-23 0.21109786665242325 1106 24-25 0.2566651272904892 1106 26-27 0.30338970818723254 1106 28-29 0.3301578647018246 1106 30-31 0.3220114139011514 1106 32-33 0.33225914572044957 1106 34-35 0.3467285545213201 1106 36-37 0.25675028409276024 1106 38-39 0.32612471447502855 1106 40-41 0.3457025818317945 1106 42-43 0.3849788628079196 1106 44-45 0.45374412200472847 1106 46-47 0.42158745946108667 1106 48-49 0.27660876422019953 1106 50-51 0.45514075220684447 1106 52-53 0.41880455370586134 1106 54-55 0.42035163747868864 1106 56-57 0.48933030026744007 1106 58-59 0.45929252271019294 1106 60-61 0.32307221877763936 1106 62-63 0.42519542642890684 1106 64-65 0.4320478132721277 1106 66-67 0.35981202882782526 1106 68-69 0.33342322743042985 1106 70-71 0.4088732038784215 1106 72-73 0.5245073559953184 1106 74-75 0.39527191097854697 1106 76-77 0.5287073556557935 1106 78-79 0.5099786383826626 1106 80-81 0.4940952418227873 1106 82-83 0.5698807667134069 1106 84-85 0.4871505617059242 1106 86-87 0.6055167050070622 1106 88-89 0.8087164487179379 1106 90-91 0.8746542686736838 1106 92-93 0.9054113035416513 1106 94-95 0.7408587558954984 1106 96-97 0.6817371837124142 1106 98-99 0.8885307207629616 1106 100-101 0.646584046882861 1107 1 -0.05642144475806532 1107 2 0.04501572824457867 1107 3 0.040493636736272265 1107 4 0.007770286788904457 1107 5 0.019833006411261067 1107 6 0.01442834661137482 1107 7 0.03885746989217154 1107 8 0.033529913235845754 1107 9 0.0817893311437885 1107 10-11 0.19413702081756412 1107 12-13 0.17507635876631156 1107 14-15 0.1902440267218637 1107 16-17 0.0670343084274947 1107 18-19 0.2011025854585924 1107 20-21 0.10894574339076968 1107 22-23 0.13242312684810997 1107 24-25 0.09040834973802703 1107 26-27 0.21492477088508366 1107 28-29 0.15283567907398066 1107 30-31 0.2634907540515883 1107 32-33 0.2721404776283549 1107 34-35 0.22434029924269794 1107 36-37 0.2335623854895772 1107 38-39 0.29414777053795405 1107 40-41 0.23122846237429684 1107 42-43 0.1918371945832007 1107 44-45 0.23478982663847603 1107 46-47 0.2814884788305463 1107 48-49 0.3711669069510961 1107 50-51 0.3257020845621952 1107 52-53 0.39051900880901513 1107 54-55 0.20923717055026714 1107 56-57 0.22401299176076606 1107 58-59 0.25459805389611745 1107 60-61 0.19232238536999802 1107 62-63 0.22233227506077213 1107 64-65 0.2585185002079342 1107 66-67 0.30737498635755856 1107 68-69 0.409763277192404 1107 70-71 0.38771557474736795 1107 72-73 0.2725845525775057 1107 74-75 0.17851247993340635 1107 76-77 0.12865637919285433 1107 78-79 0.37791524219966277 1107 80-81 0.3797026207856504 1107 82-83 0.39930377387727845 1107 84-85 0.43507706611083563 1107 86-87 0.5974156022215737 1107 88-89 0.6537711725102007 1107 90-91 0.7322709352604484 1107 92-93 0.5596127069124925 1107 94-95 0.6273394463628996 1107 96-97 0.8320226678849423 1107 98-99 0.9560601520797682 1107 100-101 0.8963830973463622 1108 1 -0.015123735901944713 1108 2 -5.200242807674726E-4 1108 3 0.03738726763074851 1108 4 -0.01070404371164102 1108 5 -0.018123869773617685 1108 6 -0.011526092725716808 1108 7 0.010773847904253842 1108 8 -0.02310472828618515 1108 9 -0.041775371622840396 1108 10-11 0.014906450264788873 1108 12-13 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-1.1829752655044032 2316 98-99 -1.306214280476528 2316 100-101 -0.686022213942163 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 12.0 4 23.0 5 51.0 6 149.0 7 317.0 8 733.0 9 1331.0 10 2287.0 11 3667.0 12 5394.0 13 7903.0 14 9393.0 15 12822.0 16 17593.0 17 25196.0 18 23307.0 19 13819.0 20 11984.0 21 12163.0 22 12534.0 23 14059.0 24 15920.0 25 18660.0 26 22246.0 27 26648.0 28 32924.0 29 39997.0 30 48444.0 31 59175.0 32 73770.0 33 94478.0 34 128545.0 35 185253.0 36 285292.0 37 471245.0 38 438190.0 39 40481.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.66662263674431 20.2446172257149 15.360691108544767 21.728069028996032 2 15.442236094561757 21.44916834762749 40.24964645323837 22.858949104572385 3 18.843474215552902 26.31603114829298 30.555902132133955 24.284592504020157 4 12.654307101686497 17.70808934471053 37.53880433708765 32.09879921651533 5 13.108340456834828 38.56964418979698 33.60352391849941 14.718491434868778 6 30.167035480835192 37.176514569280556 17.84704980359544 14.809400146288814 7 27.41050709899634 34.485060127300024 20.671295899043095 17.433136874660544 8 26.17600390350875 35.088118834383344 19.874082158284125 18.861795103823777 9 27.20127791674339 16.999186923616737 20.68066506215883 35.11887009748104 10-11 24.729906043991466 27.569249479014236 26.894484206698582 20.806360270295716 12-13 25.98940913511957 25.18226964729739 27.728386105994918 21.099935111588124 14-15 22.784917571235916 26.050527551047626 27.012097901043735 24.152456976672724 16-17 21.160450714599293 29.464422169124887 27.538098936525234 21.83702817975059 18-19 21.14135423367899 27.256333345547258 30.968307646211123 20.634004774562626 20-21 22.46242942399591 26.310882746778887 30.762023720680205 20.464664108545 22-23 21.674793565332983 26.479249390888445 30.665224495816112 21.18073254796246 24-25 21.303334983760493 26.69329768101456 31.048338189918173 20.955029145306778 26-27 21.266223789441216 26.92054995935718 30.879658994585984 20.933567256615618 28-29 20.98555711038312 26.937016496684386 31.11332095552477 20.964105437407728 30-31 21.21844111040353 26.76287529411983 30.99384093480129 21.02484266067535 32-33 21.098891516686617 26.826302673133558 30.682803272157027 21.3920025380228 34-35 21.520414671196818 26.878161346057954 30.585641893850667 21.015782088894557 36-37 21.731275080605876 26.59532369928789 30.48103732226114 21.192363897845098 38-39 21.284407244379885 26.83871168246592 30.570556724596624 21.306324348557563 40-41 21.393909662035764 26.83559659542291 30.610901002144388 21.15959274039694 42-43 21.51291535166085 26.879577008781546 30.139399893500762 21.468107746056837 44-45 21.427006027143364 27.095289811463054 29.99172308489369 21.48598107649989 46-47 21.631855762891142 27.276716130826195 29.3735567928746 21.71787131340806 48-49 21.781077908301867 27.272311943039202 29.16465098244024 21.781959166218694 50-51 21.778735617522933 27.594272565652556 28.87828854146713 21.748703275357386 52-53 21.90554399355476 27.780890525039553 28.6247181713991 21.68884731000659 54-55 21.57929303634632 27.649930960399516 28.697421949537315 22.073354053716844 56-57 21.691560656750504 27.98485535078935 28.501434827034583 21.82214916542556 58-59 21.757564372830178 27.44957664558591 28.769106607390093 22.023752374193815 60-61 21.688517370364384 27.550671824779393 28.953413484919572 21.80739731993665 62-63 21.61430129227036 27.49642504799457 28.947904264107205 21.941369395627866 64-65 21.476000332095087 27.40206557579305 28.941669538485225 22.180264553626632 66-67 21.44893643764938 27.592138993853922 28.634249671499518 22.32467489699718 68-69 21.535068149287433 27.506769474239817 28.723535506924392 22.234626869548357 70-71 21.50687253784018 27.64502088001972 28.641723815096277 22.206382767043824 72-73 21.5976139246172 27.560436899845964 28.487404273358784 22.354544902178052 74-75 21.395295659711998 27.91458662742131 28.15465983676321 22.535457876103486 76-77 21.391257859442373 27.697732529084295 28.467882337900818 22.443127273572514 78-79 21.41565735579026 27.75718932527647 28.33369433986593 22.49345897906734 80-81 21.32621329367311 28.001528770784333 28.254012501504537 22.418245434038017 82-83 21.451487447408617 27.924419610493278 28.085921719250706 22.5381712228474 84-85 21.28669221696199 27.70303834538724 28.0887974029793 22.92147203467147 86-87 21.271919133388955 27.5654910066957 28.21166058198442 22.95092927793092 88-89 21.337271573314613 27.50953411871601 28.15822191769727 22.994972390272103 90-91 21.172632599075474 27.647850407082313 28.277872605647996 22.901644388194217 92-93 21.109025982730127 27.503108753256594 28.313100733809556 23.074764530203723 94-95 20.971062736751097 27.35635611910711 28.346843635624897 23.325737508516895 96-97 21.079155977549256 27.25566080661073 28.235202172161618 23.42998104367839 98-99 20.941099967578985 27.235020818558738 28.067647212975455 23.756232000886822 100-101 20.91776118589569 26.96113070107595 28.660637776074527 23.460470336953836 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1122.0 1 767.0 2 527.5 3 693.5 4 637.5 5 646.5 6 1148.0 7 2622.0 8 4207.5 9 4673.0 10 4032.5 11 3420.5 12 3465.5 13 3659.5 14 3886.0 15 4113.0 16 4351.0 17 4498.0 18 4657.5 19 4897.0 20 5238.5 21 5838.5 22 6816.5 23 8094.5 24 9436.0 25 11138.5 26 13988.0 27 17544.0 28 20363.5 29 23658.5 30 27973.5 31 32189.0 32 37478.0 33 42752.0 34 48036.0 35 52211.5 36 56756.0 37 61991.5 38 64471.0 39 65432.5 40 66117.5 41 68874.0 42 71926.5 43 74384.5 44 77017.5 45 79911.0 46 80853.5 47 80605.0 48 85925.5 49 85061.5 50 77018.0 51 71347.5 52 66951.5 53 65061.5 54 61987.5 55 56114.0 56 49212.5 57 43687.0 58 40195.0 59 35848.5 60 30540.5 61 25850.5 62 22066.0 63 16991.5 64 12214.0 65 9638.5 66 7169.5 67 5784.5 68 4472.5 69 2854.0 70 2176.0 71 1801.0 72 1562.5 73 1474.0 74 1246.5 75 1062.5 76 773.0 77 462.5 78 318.5 79 198.5 80 137.5 81 99.5 82 64.0 83 35.5 84 17.5 85 11.0 86 6.0 87 3.0 88 3.5 89 2.0 90 1.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27625116592741494 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0026205827526693997 16-17 4.870109540359062E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 6.957299343370088E-5 26-27 0.0014610328621077185 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.00389608763228725 36-37 0.02128933599071247 38-39 0.00735154630616106 40-41 0.017694731329971257 42-43 0.005797749452808407 44-45 5.797749452808406E-4 46-47 1.6233698467863538E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 6.493479387145415E-4 60-61 0.0024350547701795307 62-63 0.001669751842408821 64-65 0.0 66-67 0.0 68-69 3.2467396935727076E-4 70-71 2.319099781123363E-5 72-73 0.0 74-75 0.0 76-77 0.22884876640125343 78-79 0.0 80-81 0.0013218868752403168 82-83 0.0 84-85 0.0 86-87 0.10389567019432665 88-89 0.3558426704155688 90-91 0.3558426704155688 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 6.957299343370088E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2156009.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.040992681430005 #Duplication Level Percentage of deduplicated Percentage of total 1 69.20330742560797 31.86188970713168 2 15.371970316358293 14.15481545669223 3 6.148949866138153 8.493112673560404 4 3.045550587333995 5.608806892094774 5 1.7662408977322666 4.065974212306682 6 1.0911434094792793 3.0142395438135656 7 0.7084397309082554 2.2832087926186864 8 0.4830329670163914 1.779145383943288 9 0.3468431711750359 1.4372103515106422 >10 1.612585549264264 13.2960818782636 >50 0.13992840686257701 4.461332576512218 >100 0.07726884844242886 6.411626263303584 >500 0.0034280852161598465 1.0534851580687126 >1k 0.001008260357694067 0.6656351318210633 >5k 1.0082603576940668E-4 0.34170724634457406 >10k+ 2.0165207153881337E-4 1.0717287320142888 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13039 0.6047748409213506 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10048 0.466046292014551 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7361 0.34141786977698146 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2385 0.1106210595595844 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.3190997811233625E-5 0.0 12-13 0.0 0.0 0.0 4.638199562246725E-5 0.0 14-15 0.0 0.0 0.0 4.638199562246725E-5 0.0 16-17 0.0 0.0 0.0 9.27639912449345E-5 0.0 18-19 0.0 0.0 0.0 1.1595498905616814E-4 0.0 20-21 0.0 0.0 0.0 1.6233698467863538E-4 0.0 22-23 0.0 0.0 0.0 3.478649671685044E-4 0.0 24-25 0.0 0.0 0.0 8.580669190156442E-4 0.0 26-27 0.0 0.0 0.0 0.001808897829276223 0.0 28-29 0.0 0.0 0.0 0.004591817566624258 0.0 30-31 0.0 0.0 0.0 0.012778239793989728 0.0 32-33 0.0 0.0 0.0 0.02803791635378145 0.0 34-35 0.0 0.0 0.0 0.049999791281019695 0.0 36-37 0.0 0.0 0.0 0.08886790361264726 0.0 38-39 0.0 4.638199562246725E-5 0.0 0.14832962200065028 0.0 40-41 0.0 4.638199562246725E-5 0.0 0.21511969569700312 0.0 42-43 0.0 4.638199562246725E-5 0.0 0.2845999251394591 0.0 44-45 0.0 4.638199562246725E-5 0.0 0.3688760111854821 0.0 46-47 0.0 4.638199562246725E-5 0.0 0.4704989635943078 4.638199562246725E-5 48-49 0.0 4.638199562246725E-5 0.0 0.5842044258627863 4.638199562246725E-5 50-51 0.0 4.638199562246725E-5 0.0 0.7078356351944728 4.638199562246725E-5 52-53 0.0 4.638199562246725E-5 0.0 0.83204661947144 4.638199562246725E-5 54-55 0.0 4.638199562246725E-5 0.0 0.9730710771615518 4.638199562246725E-5 56-57 0.0 4.638199562246725E-5 0.0 1.1376575886278768 4.638199562246725E-5 58-59 0.0 4.638199562246725E-5 0.0 1.3230696161286897 4.638199562246725E-5 60-61 0.0 4.638199562246725E-5 0.0 1.535939785038003 4.638199562246725E-5 62-63 0.0 4.638199562246725E-5 0.0 1.7509435257459502 4.638199562246725E-5 64-65 0.0 4.638199562246725E-5 0.0 1.9800242021253158 4.638199562246725E-5 66-67 0.0 4.638199562246725E-5 0.0 2.2443783861755677 4.638199562246725E-5 68-69 0.0 4.638199562246725E-5 0.0 2.5405738102206437 4.638199562246725E-5 70-71 0.0 4.638199562246725E-5 0.0 2.8779100643828484 4.638199562246725E-5 72-73 0.0 4.638199562246725E-5 0.0 3.2168464973940276 4.638199562246725E-5 74-75 0.0 4.638199562246725E-5 0.0 3.578069479301803 4.638199562246725E-5 76-77 0.0 4.638199562246725E-5 0.0 3.970901791226289 4.638199562246725E-5 78-79 0.0 4.638199562246725E-5 0.0 4.40856230191989 4.638199562246725E-5 80-81 0.0 4.638199562246725E-5 0.0 4.8927671452206365 4.638199562246725E-5 82-83 0.0 4.638199562246725E-5 0.0 5.399235346420168 4.638199562246725E-5 84-85 0.0 4.638199562246725E-5 0.0 5.9092749612826285 4.638199562246725E-5 86-87 0.0 4.638199562246725E-5 0.0 6.444430426774656 4.638199562246725E-5 88-89 0.0 4.638199562246725E-5 0.0 7.020587576396945 4.638199562246725E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9040 0.0 37.213493 1 GTATCAA 14290 0.0 35.329105 1 TATCACG 80 4.5213287E-4 29.657831 2 ATCAACG 18090 0.0 27.831472 3 TCAACGC 18235 0.0 27.58414 4 TATCAAC 18415 0.0 27.262978 2 TAGATCG 70 0.008434208 27.11573 5 CAACGCA 18575 0.0 27.079233 5 AACGCAG 19200 0.0 26.271894 6 ATCGGAC 95 0.0012340755 24.975016 3 CCCGTAG 175 1.4625948E-7 24.471123 1 GTACATG 13965 0.0 24.089506 1 TACATGG 13815 0.0 23.391365 2 ACGCAGA 21660 0.0 23.200474 7 CGCAGAG 21750 0.0 23.03902 8 CCGTAGG 165 2.4527399E-6 23.007288 2 ACATGGG 13945 0.0 22.186481 3 GAGTACT 10855 0.0 22.164484 12-13 GCAGAGT 22940 0.0 21.555285 9 AGTACTT 11255 0.0 21.271355 12-13 >>END_MODULE