FastQCFastQC Report
Fri 27 May 2016
SRR939245_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939245_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences811271
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79010.9739039112700935No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56650.6982870089033134No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46110.5683674136016201No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39100.48195978902241055No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21810.2688374168434469No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT17930.22101122806066037No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14430.1778690474576313No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13220.1629541793062984No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12170.15001152512538965No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT12040.14840910127442No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA10610.13078243891375385No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC9630.1187026283449057No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8700.10723913464181513No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA8240.1015690194768456No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCGA650.00591151829.161186
TTCCGAT700.00848824427.0782387
CATTCCG2305.456968E-1226.783919
GTATCAA88750.025.8337971
GAGTACT46350.024.7413912-13
AGTACTT48050.023.96466312-13
ATGGGCG1000.001667907723.7139455
GGTATCA63900.023.1030351
CTATACG1452.3596733E-522.8976384
GTACTTT50400.022.7588614-15
TCAACGC103750.021.8981824
CAACGCA104300.021.781365
TATCAAC105800.021.742862
ATCAACG104850.021.6684443
GTACATG51150.021.5303881
GTATTAT4200.021.4740431
GTGGTAC2005.4160046E-721.3610211
CGTGCAA2005.506863E-721.3241129
AACGCAG106750.021.2630776
CCTATAC2901.07320375E-1021.2620933
Produced by FastQC (version 0.11.5)