##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939245_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 811271 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.53541911395822 34.0 31.0 34.0 31.0 34.0 2 32.64105829001653 34.0 31.0 34.0 31.0 34.0 3 32.67400658965993 34.0 31.0 34.0 31.0 34.0 4 36.126676535954076 37.0 37.0 37.0 35.0 37.0 5 36.16129628693741 37.0 37.0 37.0 35.0 37.0 6 36.26771078961284 37.0 37.0 37.0 35.0 37.0 7 36.23795994186899 37.0 37.0 37.0 35.0 37.0 8 36.23246362806017 37.0 37.0 37.0 35.0 37.0 9 37.98801633486221 39.0 39.0 39.0 37.0 39.0 10-11 37.925527351526185 39.0 38.0 39.0 37.0 39.0 12-13 37.86879415139947 39.0 38.0 39.0 35.0 39.0 14-15 39.22276156795941 41.0 39.0 41.0 36.5 41.0 16-17 39.16890471864519 40.0 39.0 41.0 36.5 41.0 18-19 39.156161134811924 40.0 39.0 41.0 36.0 41.0 20-21 39.161317858027715 40.5 39.0 41.0 36.0 41.0 22-23 39.173375481189396 41.0 39.0 41.0 36.0 41.0 24-25 39.1132186408734 41.0 39.0 41.0 36.0 41.0 26-27 39.02655771006236 41.0 39.0 41.0 35.5 41.0 28-29 38.88876528311748 40.0 39.0 41.0 35.0 41.0 30-31 38.68077189989535 40.0 38.0 41.0 35.0 41.0 32-33 38.426594196020815 40.0 38.0 41.0 35.0 41.0 34-35 38.16811953095821 40.0 38.0 41.0 35.0 41.0 36-37 37.93962683246412 40.0 38.0 41.0 34.5 41.0 38-39 37.42250246834905 39.5 36.5 41.0 33.5 41.0 40-41 37.40615836138602 40.0 37.0 41.0 33.0 41.0 42-43 37.48489407362028 40.0 37.0 41.0 33.0 41.0 44-45 37.30708480889863 40.0 37.5 41.0 33.0 41.0 46-47 36.9306890052769 40.0 37.0 41.0 32.5 41.0 48-49 36.61963449944593 40.0 37.0 41.0 31.0 41.0 50-51 35.519686393326026 39.0 35.5 40.0 29.5 40.5 52-53 35.64648557633639 39.0 35.5 40.0 30.0 40.5 54-55 36.14430134936414 40.0 36.0 41.0 31.0 41.0 56-57 36.082794775112134 40.0 36.0 41.0 30.5 41.0 58-59 35.90056097161121 39.5 36.0 41.0 30.0 41.0 60-61 35.63708366254926 39.0 35.0 41.0 29.0 41.0 62-63 35.29080664784024 39.0 35.0 40.5 28.0 41.0 64-65 34.93947645114888 38.5 35.0 40.0 27.0 41.0 66-67 34.57943091765883 38.0 35.0 40.0 26.0 41.0 68-69 34.17005969645162 37.0 35.0 40.0 26.0 41.0 70-71 33.75916309100165 37.0 34.0 39.0 25.5 41.0 72-73 33.295996652166785 36.0 34.0 39.0 23.5 40.5 74-75 32.82038800844601 35.5 34.0 38.5 21.5 40.0 76-77 32.34885137025729 35.0 33.0 37.0 20.0 39.5 78-79 31.940493373977375 35.0 33.0 37.0 18.0 39.0 80-81 31.512942037864043 35.0 33.0 36.5 13.0 39.0 82-83 31.06355336256319 35.0 32.0 36.0 6.5 37.5 84-85 30.625013096733397 35.0 32.0 35.5 2.0 37.0 86-87 30.22947942179617 35.0 31.5 35.0 2.0 36.5 88-89 29.798317701483228 34.0 31.0 35.0 2.0 36.0 90-91 29.413660786592892 34.0 31.0 35.0 2.0 36.0 92-93 29.013240951543935 34.0 30.0 35.0 2.0 35.5 94-95 28.5831140272486 34.0 29.5 35.0 2.0 35.0 96-97 28.067027540735463 34.0 29.0 35.0 2.0 35.0 98-99 27.418837231948388 34.0 28.5 35.0 2.0 35.0 100-101 24.712494345292754 31.5 21.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1880423730736922 1101 2 0.22844691329062528 1101 3 0.23899885063775628 1101 4 0.22194558338296133 1101 5 0.09604496658266726 1101 6 0.1858809232128067 1101 7 0.10973966000774027 1101 8 -0.02876822834850401 1101 9 0.010451062798708222 1101 10-11 0.1769116362767349 1101 12-13 0.1343268365071637 1101 14-15 0.17451307366254554 1101 16-17 0.27831218506238287 1101 18-19 0.2474026852610436 1101 20-21 0.16185047456559687 1101 22-23 0.31189882746606656 1101 24-25 0.19470130025582222 1101 26-27 0.2305969638842953 1101 28-29 0.3539313385587448 1101 30-31 0.3286200056818842 1101 32-33 0.2917862023654436 1101 34-35 0.32852909917403395 1101 36-37 0.22019128276181021 1101 38-39 0.6417444341586531 1101 40-41 0.4720236883518041 1101 42-43 0.4546388748268626 1101 44-45 0.3147019409900196 1101 46-47 0.46278075745142644 1101 48-49 0.5057240394494471 1101 50-51 0.360011078941163 1101 52-53 0.47694559476644116 1101 54-55 0.7268115138635025 1101 56-57 0.7068738777625896 1101 58-59 0.70649556574336 1101 60-61 0.7358548501678968 1101 62-63 0.6823406991520713 1101 64-65 0.7955363167703027 1101 66-67 0.5337834600894027 1101 68-69 0.6858008691016337 1101 70-71 0.6620230609484778 1101 72-73 0.5168889216128321 1101 74-75 0.5396405772802666 1101 76-77 0.481384265381827 1101 78-79 0.47990094775368064 1101 80-81 0.3844286752739805 1101 82-83 0.3041325358497886 1101 84-85 0.358168593711909 1101 86-87 0.35341441143227925 1101 88-89 0.12825289607295787 1101 90-91 0.2411287640421378 1101 92-93 0.26642058857216355 1101 94-95 0.36544249770992465 1101 96-97 0.42310564278497154 1101 98-99 0.3871952720917662 1101 100-101 0.37387975847769184 1102 1 0.23584527801945399 1102 2 0.07030663015034122 1102 3 0.25761440496759036 1102 4 0.039777445857680505 1102 5 0.03151104490589063 1102 6 0.14106569982615014 1102 7 0.10651066570731871 1102 8 0.20606354219754763 1102 9 0.26793175777940803 1102 10-11 0.2608654880877239 1102 12-13 0.26377963113852587 1102 14-15 0.2739741418736159 1102 16-17 0.1756048859265178 1102 18-19 0.27299332692311395 1102 20-21 0.2897577185442799 1102 22-23 0.285434863502104 1102 24-25 0.1757639956042354 1102 26-27 0.25805490652081176 1102 28-29 0.21341263107574804 1102 30-31 0.13259936769695457 1102 32-33 0.17602503303285033 1102 34-35 0.1695959313122941 1102 36-37 0.2403351511199574 1102 38-39 0.4442012276868752 1102 40-41 0.28954529158769304 1102 42-43 0.22702924989579998 1102 44-45 0.18411152914960383 1102 46-47 0.17111983632622696 1102 48-49 -0.03868325049356258 1102 50-51 0.14281237513531408 1102 52-53 0.240120121333824 1102 54-55 0.14989595034079173 1102 56-57 0.1646384024913985 1102 58-59 0.2902081048130398 1102 60-61 0.3610501985775372 1102 62-63 0.38951343311051545 1102 64-65 0.18976089849488886 1102 66-67 0.25766422861303084 1102 68-69 0.25609756886261437 1102 70-71 0.3308098324138129 1102 72-73 0.22003977654940599 1102 74-75 0.07847469200724078 1102 76-77 0.1519061903680452 1102 78-79 0.30492622813610026 1102 80-81 0.13284290583106895 1102 82-83 0.02835803686100391 1102 84-85 -0.10376719411673818 1102 86-87 -0.018264665603940244 1102 88-89 -0.29588297449148726 1102 90-91 -0.17711792652598035 1102 92-93 -0.21243007331421282 1102 94-95 0.07089731477188721 1102 96-97 0.1270896012332159 1102 98-99 0.29203186889264643 1102 100-101 0.4153970410664023 1103 1 0.02217966435383545 1103 2 0.07422613406984624 1103 3 0.08695803431122329 1103 4 -0.040508034427794826 1103 5 -0.039391025996181384 1103 6 -0.047421488661036904 1103 7 0.01750867670533296 1103 8 0.04776238389639076 1103 9 -0.01016582031817137 1103 10-11 0.08465746187969359 1103 12-13 0.06701468437358216 1103 14-15 0.14602281392228633 1103 16-17 0.0625886229102548 1103 18-19 -0.011340357410574597 1103 20-21 0.0979126266991841 1103 22-23 0.08446376253100141 1103 24-25 0.09609276593300109 1103 26-27 0.028219876685781742 1103 28-29 -0.06797265030953525 1103 30-31 -0.04880346370586608 1103 32-33 0.02439288140069351 1103 34-35 0.015565277281638146 1103 36-37 -0.19694693616212788 1103 38-39 0.19566372914938057 1103 40-41 0.18361923566164506 1103 42-43 0.05372282658937877 1103 44-45 0.11088700592507905 1103 46-47 0.1955845607909481 1103 48-49 -0.027404439214748777 1103 50-51 -0.008854876531934508 1103 52-53 0.10483288604658725 1103 54-55 0.30599750644234547 1103 56-57 0.29890773676073223 1103 58-59 0.2306374952424335 1103 60-61 0.26124909877643177 1103 62-63 0.2088945024915887 1103 64-65 0.11842592715991174 1103 66-67 0.09340776435656295 1103 68-69 0.22572433848938545 1103 70-71 0.10071740232138637 1103 72-73 -0.11142740491777658 1103 74-75 -0.013153949621404593 1103 76-77 0.09111292957478412 1103 78-79 0.020276643486518253 1103 80-81 0.20070882369698495 1103 82-83 0.0625045210074866 1103 84-85 0.2745994842499435 1103 86-87 0.17342832608905212 1103 88-89 0.160581281972771 1103 90-91 0.04897894957089477 1103 92-93 0.10196094107680054 1103 94-95 -0.0647175208429509 1103 96-97 0.06400313814675229 1103 98-99 -0.01849814163736241 1103 100-101 -0.01181133614197094 1104 1 0.21775952309159408 1104 2 0.2519431538921282 1104 3 0.17893798418590734 1104 4 0.24712012688457463 1104 5 0.12773132533669695 1104 6 0.18292711010861495 1104 7 0.31652895467297526 1104 8 0.2232009803876167 1104 9 0.25465532345033637 1104 10-11 0.3028962406447917 1104 12-13 0.2851167577388125 1104 14-15 0.2195475348154261 1104 16-17 0.34423592297860495 1104 18-19 0.34857078407952 1104 20-21 0.39710378641665756 1104 22-23 0.28636624601243454 1104 24-25 0.3763160514194439 1104 26-27 0.3582039277224638 1104 28-29 0.2572722801985563 1104 30-31 0.35320154882545296 1104 32-33 0.4000479281083713 1104 34-35 0.34166461653887836 1104 36-37 0.4184324213224926 1104 38-39 0.7770604026513155 1104 40-41 0.4495568068623683 1104 42-43 0.341487693301616 1104 44-45 0.26387174048876716 1104 46-47 0.1748623005949952 1104 48-49 0.26388059154396615 1104 50-51 0.32057096078863623 1104 52-53 0.3328688851352197 1104 54-55 0.4379979621270209 1104 56-57 0.4213274223383152 1104 58-59 0.34610798966029677 1104 60-61 0.47388295614713627 1104 62-63 0.47689404680692604 1104 64-65 0.35302378544198376 1104 66-67 0.47249183817748275 1104 68-69 0.394145391120972 1104 70-71 0.4436087215284985 1104 72-73 0.43518913643561064 1104 74-75 0.5041164992279974 1104 76-77 0.6117212686041711 1104 78-79 0.4760296623974334 1104 80-81 0.3875167526101748 1104 82-83 0.17700668550965304 1104 84-85 0.331036030160174 1104 86-87 0.4161829398962986 1104 88-89 0.32826184702176064 1104 90-91 0.39583472537404063 1104 92-93 0.24591172713284948 1104 94-95 0.4503086810253585 1104 96-97 0.5578286109195929 1104 98-99 0.31186184924894533 1104 100-101 0.03300525077987615 1105 1 -0.07104174837976984 1105 2 -0.006309184270349988 1105 3 0.08260263361435705 1105 4 -0.0032099666419256323 1105 5 -0.02535871684192159 1105 6 -0.04381046553781687 1105 7 -0.00710329668712717 1105 8 0.043755415255276375 1105 9 0.10518102795550277 1105 10-11 9.783361273960622E-4 1105 12-13 0.05635583103180153 1105 14-15 0.0802404255789142 1105 16-17 0.25948441004994294 1105 18-19 0.12260801129389876 1105 20-21 0.20543559153922075 1105 22-23 0.251315203772684 1105 24-25 0.2504877611816596 1105 26-27 0.2233576657260059 1105 28-29 0.2382674510525149 1105 30-31 0.22863556068437418 1105 32-33 0.1448474268552431 1105 34-35 0.12298529628702681 1105 36-37 0.20504071033771964 1105 38-39 0.5867232793552688 1105 40-41 0.4324393180183037 1105 42-43 0.256850796180764 1105 44-45 0.3161610002234667 1105 46-47 0.37703847273266433 1105 48-49 0.3293583102309299 1105 50-51 0.2410184209023356 1105 52-53 0.27258708306907664 1105 54-55 0.25720909972711326 1105 56-57 0.5230604133524324 1105 58-59 0.6060413596706837 1105 60-61 0.41112239621071467 1105 62-63 0.43433796147163406 1105 64-65 0.5189248185124669 1105 66-67 0.5881575267892529 1105 68-69 0.5205849656670907 1105 70-71 0.39899107986335736 1105 72-73 0.4765516891588817 1105 74-75 0.16799112481635348 1105 76-77 0.08097672431662062 1105 78-79 0.2540983096252525 1105 80-81 0.06184281166024164 1105 82-83 0.10595558214147616 1105 84-85 0.1473267569772112 1105 86-87 0.22033203213910113 1105 88-89 0.10062721165284572 1105 90-91 0.07496089445528042 1105 92-93 0.07502872694946561 1105 94-95 0.42563724634108624 1105 96-97 0.1377677881309154 1105 98-99 0.20843407248997536 1105 100-101 -0.09856299911315247 1106 1 0.05507475939442941 1106 2 0.14136306618828343 1106 3 0.11700911762987687 1106 4 0.012980523869636329 1106 5 0.1426993866737618 1106 6 0.059789426194761575 1106 7 0.14598082108376076 1106 8 0.203478431104422 1106 9 0.06440781812722918 1106 10-11 0.137054694733159 1106 12-13 0.24551405183718344 1106 14-15 0.2173889323907474 1106 16-17 0.18912712756219463 1106 18-19 0.18806393743145122 1106 20-21 0.13694892948394966 1106 22-23 0.1364870783027783 1106 24-25 0.18567993585309495 1106 26-27 0.17008257625501955 1106 28-29 0.1338224719541401 1106 30-31 0.15384758438290191 1106 32-33 0.09327529368631104 1106 34-35 -0.08766626306457681 1106 36-37 -0.17677863344142253 1106 38-39 0.33599472983796375 1106 40-41 0.15085304993614557 1106 42-43 0.11229649706194067 1106 44-45 -0.026207650343437194 1106 46-47 -0.16743688139202106 1106 48-49 -0.085940477775452 1106 50-51 -0.12834340280221568 1106 52-53 -0.16029914591651817 1106 54-55 -0.0106181836628636 1106 56-57 0.2088217438387332 1106 58-59 0.10966531083005293 1106 60-61 0.05557068348026917 1106 62-63 -0.129492449643827 1106 64-65 0.001465945095127097 1106 66-67 -0.20318934999639993 1106 68-69 -0.09546643128337706 1106 70-71 -0.1402453340790828 1106 72-73 -0.25136927562412836 1106 74-75 -0.3306607954266454 1106 76-77 -0.24811713752212228 1106 78-79 -0.08390129940905666 1106 80-81 0.03961941988254836 1106 82-83 -0.013381430279203244 1106 84-85 0.022419235398917436 1106 86-87 0.055361805679748244 1106 88-89 0.14198311072355452 1106 90-91 0.03343056454039228 1106 92-93 -0.041170033990056254 1106 94-95 0.06629918132048473 1106 96-97 -0.003506910374394323 1106 98-99 0.09010755599422993 1106 100-101 -0.05204785671141465 1107 1 0.14620563837981138 1107 2 0.0724945323382471 1107 3 0.103624700977889 1107 4 0.10213265821289497 1107 5 0.1188340822289291 1107 6 0.12465643341688804 1107 7 0.13125326544992078 1107 8 0.05252428865829728 1107 9 0.2586653485129986 1107 10-11 0.25505789478012986 1107 12-13 0.2716142514731459 1107 14-15 0.278382121281588 1107 16-17 0.3365604843821117 1107 18-19 0.3139843179141053 1107 20-21 0.2611702024567535 1107 22-23 0.17142263698987392 1107 24-25 0.257456402296647 1107 26-27 0.41193199789790214 1107 28-29 0.06638882155193215 1107 30-31 0.24643463153222456 1107 32-33 0.20258552209333658 1107 34-35 0.20463454135089876 1107 36-37 0.24255522834003074 1107 38-39 0.5583260668117163 1107 40-41 0.3051019196443221 1107 42-43 0.08418819455474846 1107 44-45 0.1066662267043057 1107 46-47 0.12583347853986027 1107 48-49 0.19088560407529087 1107 50-51 0.13097196329490401 1107 52-53 0.1860017172154187 1107 54-55 0.2656295410743823 1107 56-57 0.21822591857891638 1107 58-59 0.3203561099610468 1107 60-61 0.450480700508038 1107 62-63 0.49152437262146265 1107 64-65 0.4741077453417333 1107 66-67 0.47825624920505305 1107 68-69 0.35251005277510217 1107 70-71 0.4207390473430266 1107 72-73 0.4463106903203169 1107 74-75 0.4248871759197286 1107 76-77 0.5445220204838748 1107 78-79 0.5270948253047045 1107 80-81 0.4259252739134354 1107 82-83 0.5883162093191778 1107 84-85 0.40982675697721405 1107 86-87 0.365149105309829 1107 88-89 0.3948345287260189 1107 90-91 0.41889236948431474 1107 92-93 0.5420583436742028 1107 94-95 0.4426958990704719 1107 96-97 0.6171957788393918 1107 98-99 0.5277139795747594 1107 100-101 0.5666518673212302 1108 1 0.06561843417297553 1108 2 0.15590090102068643 1108 3 0.10080553650228552 1108 4 0.10921708707646616 1108 5 0.17675111407233857 1108 6 0.21646186724685634 1108 7 0.2937496136824649 1108 8 0.18045300615756332 1108 9 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2316 56-57 -0.7411952636870964 2316 58-59 -0.8019345304330443 2316 60-61 -0.6343920416922941 2316 62-63 -0.8195798966724652 2316 64-65 -0.9835071971870022 2316 66-67 -1.0774087754254893 2316 68-69 -0.9115498815434506 2316 70-71 -0.6559850905879969 2316 72-73 -0.7973058942445448 2316 74-75 -0.5820299012559715 2316 76-77 -0.5440863539003651 2316 78-79 -0.6205652732174691 2316 80-81 -0.6874983714067611 2316 82-83 -0.645817616624992 2316 84-85 -0.4589950821032467 2316 86-87 -0.3467761820464901 2316 88-89 -0.39324018391765847 2316 90-91 -0.272158533635551 2316 92-93 -0.40512966946208806 2316 94-95 -0.4524395689098242 2316 96-97 -0.22970487797505612 2316 98-99 -0.20393626673066123 2316 100-101 0.6792544945790304 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3269.0 3 456.0 4 399.0 5 361.0 6 498.0 7 523.0 8 611.0 9 770.0 10 755.0 11 1122.0 12 1848.0 13 3125.0 14 3394.0 15 4144.0 16 6486.0 17 9759.0 18 8292.0 19 4057.0 20 3595.0 21 3354.0 22 3485.0 23 3614.0 24 4130.0 25 4496.0 26 5122.0 27 5886.0 28 7063.0 29 8440.0 30 10559.0 31 13457.0 32 17951.0 33 25449.0 34 38837.0 35 66285.0 36 102374.0 37 186107.0 38 215686.0 39 35506.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.70992666414551 22.16945105628842 16.167750514735143 20.95287176483092 2 14.270245844157126 22.600204458579853 41.38928123425817 21.74026846300485 3 17.784288596354763 28.231189208009543 31.517259431015365 22.467262764620326 4 11.50051247854382 19.035675915978217 40.27636794724558 29.187443658232382 5 11.5475058355667 39.661228371351996 35.27955143326459 13.51171435981672 6 27.021578224611442 39.809227223430675 18.87073669976175 14.298457852196133 7 25.027394961301532 35.54212407202654 22.861712209761492 16.56876875691044 8 24.304571685269014 35.200767130861465 21.37883627136884 19.115824912500678 9 25.67800061709349 18.115643319962974 22.8686207960506 33.337735266892935 10-11 22.933480430629803 28.440250361573057 28.57692003929176 20.04934916850538 12-13 24.546791121344544 27.18093373090717 28.57756299083618 19.694712156912104 14-15 22.00652840597059 28.067085850389667 28.178833895878714 21.74755184776103 16-17 19.081434924794706 31.179798675231947 29.147488252353693 20.591278147619654 18-19 18.728410160969645 29.752294585678722 32.72387097251928 18.795424280832357 20-21 20.030737040911742 28.601557219964263 32.21957850752533 19.148127231598664 22-23 19.785729540485722 28.476725539503068 32.17309774322851 19.564447176782696 24-25 19.367926898759773 29.12239370630968 32.482786253203365 19.026893141727182 26-27 19.129865287942085 29.043583893810553 32.791127176478724 19.035423641768638 28-29 18.714643277091774 29.064653615991652 32.979424046120755 19.24127906079582 30-31 19.104676955664647 29.146695370197783 32.58889721411987 19.159730460017702 32-33 18.91445307557226 29.498643606432918 32.27513031562769 19.31177300236713 34-35 19.455922460479936 29.17525843312148 32.19032697455023 19.178492131848344 36-37 19.481361947931926 28.837186846 32.24149958620621 19.439951619861855 38-39 18.952990183702866 29.582733118493547 32.23760092272203 19.226675775081556 40-41 18.99834063199524 29.289392047655166 32.63677411930374 19.07549320104586 42-43 18.874664194552725 29.396104068900687 31.784445856805938 19.94478587974065 44-45 19.162168417899586 29.407097599104848 31.587979804606224 19.84275417838934 46-47 19.641198266829313 29.662692702598825 31.034858367574035 19.66125066299782 48-49 19.38446816122846 29.688291141199326 31.066339181923635 19.860901515648578 50-51 19.070430990997682 29.87983499360956 31.014083722484752 20.035650292908006 52-53 19.250300201785116 30.32421792792681 30.66582899020785 19.75965288008022 54-55 19.259065805531694 29.78639132791865 30.846513170760687 20.10802969578897 56-57 19.447367460551654 30.060297646554464 30.46296244743984 20.029372445454044 58-59 19.49590571072631 29.585709142778672 30.897873493327527 20.020511653167492 60-61 19.46460149632103 30.011315154887775 30.78989674148272 19.734186607308477 62-63 19.262950574521977 29.853726531996706 31.114493403086403 19.768829490394925 64-65 19.243029318702867 29.703074477725018 30.969582031462767 20.08431417210935 66-67 19.139424454820464 29.84616927384296 30.390281012976356 20.624125258360223 68-69 19.043701182001485 29.57900986249707 30.919432852249507 20.45785610325194 70-71 19.437009102344422 29.666375305805097 30.763187999564195 20.13342759228628 72-73 19.516783276086084 29.165085635654936 30.83601618563089 20.48211490262809 74-75 19.122723938456968 29.949085551748578 30.362653246299033 20.565537263495422 76-77 19.21688055544038 29.49319592962557 30.644216029621173 20.645707485312876 78-79 19.16911754241258 29.332233558919096 30.820641684956705 20.67800721371162 80-81 19.327036417130994 29.68353510071869 30.32562255009637 20.663805932053943 82-83 19.426482573736497 29.47993190269586 30.471748963286 20.621836560281647 84-85 19.194323354894287 29.12461538080704 30.446368674979148 21.234692589319533 86-87 19.213672209326 29.37933313571274 30.46863928321898 20.938355371742276 88-89 19.572925544194383 28.898081804155005 30.24180296669569 21.287189684954917 90-91 19.308485613826573 28.695897093052047 30.787401574803148 21.20821571831823 92-93 19.827754312177976 28.943887054768357 30.4883038962663 20.740054736787368 94-95 19.551268757749636 28.475194054224374 30.59832276731631 21.375214420709682 96-97 19.50831484489521 28.2692345786617 30.561078701319282 21.661371875123805 98-99 19.531256286881753 28.432026157142175 30.10039821302447 21.936319342951606 100-101 19.640940772804186 27.830370261261137 30.829420873412065 21.699268092522612 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3579.0 1 2109.0 2 774.5 3 939.0 4 814.0 5 844.5 6 1508.0 7 3052.0 8 4529.0 9 4530.5 10 3419.5 11 2581.5 12 2411.0 13 2350.0 14 2266.5 15 2204.0 16 2179.0 17 2131.0 18 2129.0 19 2211.0 20 2440.5 21 2733.5 22 3043.0 23 3398.0 24 3777.0 25 4582.0 26 6094.5 27 7968.5 28 8761.5 29 9731.0 30 12315.5 31 14883.0 32 18445.0 33 23108.5 34 27507.0 35 30758.0 36 34458.5 37 37575.0 38 36477.0 39 34227.5 40 32306.0 41 30911.5 42 29289.5 43 28398.5 44 28768.0 45 28141.0 46 26477.5 47 26023.0 48 30847.5 49 29349.5 50 21701.5 51 17641.5 52 14944.0 53 13670.0 54 12624.5 55 11389.5 56 10017.0 57 8836.5 58 7864.5 59 6882.0 60 5810.5 61 4823.5 62 3931.5 63 2927.5 64 2215.5 65 1819.5 66 1400.5 67 1168.0 68 982.5 69 720.0 70 517.0 71 391.0 72 324.5 73 269.0 74 223.5 75 195.0 76 134.5 77 81.0 78 59.5 79 40.5 80 29.0 81 20.0 82 13.0 83 7.5 84 5.0 85 4.5 86 2.0 87 1.5 88 3.0 89 2.5 90 2.0 91 2.0 92 0.5 93 1.0 94 2.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2609485609617501 2 0.16357049617205596 3 0.16480312990357104 4 0.18255305563738874 5 0.1948793929525399 6 0.09589890431187606 7 0.11130682595581502 8 0.12067484231532989 9 0.1258519039876934 10-11 0.11377209341884523 12-13 0.1054518157311182 14-15 0.11907241846436024 16-17 0.09731643310311844 18-19 0.12252379291260258 20-21 0.1446495683932989 22-23 0.1503813152448442 24-25 0.17016508663566182 26-27 0.15370942631993503 28-29 0.1508127370508745 30-31 0.1417528791242384 32-33 0.15019642018511695 34-35 0.32042313850735454 36-37 0.431606701090018 38-39 0.3977709051599281 40-41 0.38606088471053446 42-43 0.409912347415352 44-45 0.4146579872816852 46-47 0.41755467655074563 48-49 0.43117527928398774 50-51 0.42199215798420014 52-53 0.429079801940412 54-55 0.41231598319180646 56-57 0.373549652335656 58-59 0.36362695079695934 60-61 0.323073301030112 62-63 0.4276006414625939 64-65 0.42636800773107875 66-67 0.4354278656577149 68-69 0.4456587256292903 70-71 0.43968045203144196 72-73 0.43357891506044216 74-75 0.4367837627623815 76-77 0.41114498114686704 78-79 0.3977092734733523 80-81 0.3948742158908675 82-83 0.4079401334449278 84-85 0.41034376922138227 86-87 0.409912347415352 88-89 0.4194036271480184 90-91 0.4377082380610178 92-93 0.442207351181048 94-95 0.4402351372106238 96-97 0.43869434504622995 98-99 0.4360441825234724 100-101 0.4168767279984124 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 811271.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.56259450014781 #Duplication Level Percentage of deduplicated Percentage of total 1 80.84721389784872 37.644560371922445 2 10.510202366037287 9.787645817685767 3 3.1462609585032815 4.394942196073041 4 1.3969564092692501 2.601836592767465 5 0.7993499465191345 1.8609903711742652 6 0.5194908531929288 1.4513305166254895 7 0.363420835291925 1.1845271890622038 8 0.2775165978207972 1.0337514249112307 9 0.216043995222427 0.9053612049360366 >10 1.5544707630195989 14.81328059805033 >50 0.22144412797158475 7.319339087724252 >100 0.14075046707752792 11.765259018756268 >500 0.003968528207051128 1.2571537436366693 >1k 0.002381116924230677 2.3088266010032785 >5k 5.291370942734838E-4 1.671195265671245 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7901 0.9739039112700935 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5665 0.6982870089033134 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4611 0.5683674136016201 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3910 0.48195978902241055 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2181 0.2688374168434469 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1793 0.22101122806066037 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1443 0.1778690474576313 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1322 0.1629541793062984 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1217 0.15001152512538965 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1204 0.14840910127442 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1061 0.13078243891375385 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 963 0.1187026283449057 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 870 0.10723913464181513 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 824 0.1015690194768456 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 6.163168657575582E-5 0.0 20-21 0.0 0.0 0.0 1.2326337315151165E-4 0.0 22-23 0.0 0.0 0.0 1.8489505972726748E-4 0.0 24-25 0.0 0.0 0.0 5.546851791818024E-4 0.0 26-27 0.0 0.0 0.0 0.001355897104666628 0.0 28-29 0.0 0.0 0.0 0.0036362695079695936 0.0 30-31 0.0 0.0 0.0 0.011586757076242094 0.0 32-33 0.0 0.0 0.0 0.029583209556362795 0.0 34-35 0.0 0.0 0.0 0.05522199117187722 0.0 36-37 0.0 0.0 0.0 0.09435811214748216 0.0 38-39 0.0 0.0 0.0 0.153462899573632 0.0 40-41 0.0 0.0 0.0 0.21897738240366044 0.0 42-43 0.0 0.0 0.0 0.285971025711507 0.0 44-45 0.0 0.0 0.0 0.36738648367808047 0.0 46-47 0.0 0.0 0.0 0.47012650519986543 0.0 48-49 0.0 0.0 0.0 0.5725583682887716 0.0 50-51 0.0 0.0 0.0 0.6837419308714351 0.0 52-53 0.0 0.0 0.0 0.7989931847680984 0.0 54-55 0.0 0.0 0.0 0.9387122182353369 0.0 56-57 0.0 0.0 0.0 1.1067201958408472 0.0 58-59 0.0 0.0 0.0 1.2911838337620845 0.0 60-61 0.0 0.0 0.0 1.493274134043988 0.0 62-63 0.0 0.0 0.0 1.6993704939533152 0.0 64-65 0.0 0.0 0.0 1.9240796232085209 0.0 66-67 0.0 0.0 0.0 2.18126865128915 0.0 68-69 0.0 0.0 0.0 2.4648976729107783 0.0 70-71 0.0 0.0 0.0 2.7940108792253144 0.0 72-73 0.0 0.0 0.0 3.143647437169577 0.0 74-75 0.0 0.0 0.0 3.511958396146294 0.0 76-77 0.0 0.0 0.0 3.906154663484828 0.0 78-79 0.0 0.0 0.0 4.364632779921876 0.0 80-81 0.0 0.0 0.0 4.8565152704829835 0.0 82-83 0.0 0.0 0.0 5.356718038731817 0.0 84-85 0.0 0.0 0.0 5.881326954864651 0.0 86-87 0.0 0.0 0.0 6.443223041375816 0.0 88-89 0.0 0.0 0.0 7.036428024667466 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCCGA 65 0.005911518 29.16118 6 TTCCGAT 70 0.008488244 27.078238 7 CATTCCG 230 5.456968E-12 26.78391 9 GTATCAA 8875 0.0 25.833797 1 GAGTACT 4635 0.0 24.74139 12-13 AGTACTT 4805 0.0 23.964663 12-13 ATGGGCG 100 0.0016679077 23.713945 5 GGTATCA 6390 0.0 23.103035 1 CTATACG 145 2.3596733E-5 22.897638 4 GTACTTT 5040 0.0 22.75886 14-15 TCAACGC 10375 0.0 21.898182 4 CAACGCA 10430 0.0 21.78136 5 TATCAAC 10580 0.0 21.74286 2 ATCAACG 10485 0.0 21.668444 3 GTACATG 5115 0.0 21.530388 1 GTATTAT 420 0.0 21.474043 1 GTGGTAC 200 5.4160046E-7 21.361021 1 CGTGCAA 200 5.506863E-7 21.324112 9 AACGCAG 10675 0.0 21.263077 6 CCTATAC 290 1.07320375E-10 21.262093 3 >>END_MODULE