FastQCFastQC Report
Fri 27 May 2016
SRR939245_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939245_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences811271
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77890.9600984134771241No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64720.7977605510365834No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52170.6430650177314362No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37470.4618678591987141No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28480.35105408673550514No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17090.2106571047159334No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13910.17145935205375268No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12850.15839343449969245No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT10400.1281939080775721No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA9930.12240052953945105No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9300.11463493703090583No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA8890.10958113873169384No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT8590.1058832375371485No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCGA352.0630895E-433.90224510-11
CCGAGCG753.0916993E-431.6420979
GTCCCGA1004.9776037E-528.4778866
ACACCGT856.43468E-427.9194976
TGTCCCG1205.411681E-627.6868365
CATATAG3150.025.6815091
GTAAGCG851.13188435E-725.12754694-95
GTACATG44850.024.1910421
GTGTACG1000.001660713623.7315719
TACCGCG1401.7893615E-523.7315719
GAGTACT57750.023.62883812-13
CCTATAC2252.8358045E-923.2042033
AGTACTT60150.022.37040912-13
GTACTTT60600.022.28261414-15
GTATCAA105200.022.2552011
TACATGG46750.022.234072
ACATGGG45750.022.201343
GGTATCA80700.021.4050031
GGATATG4500.021.1494791
CATGGGG17800.020.2651624
Produced by FastQC (version 0.11.5)