##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939245_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 811271 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.820441011696463 31.0 31.0 34.0 30.0 34.0 2 32.07431795294051 33.0 31.0 34.0 30.0 34.0 3 32.12045543351112 34.0 31.0 34.0 30.0 34.0 4 35.80069914985251 37.0 35.0 37.0 35.0 37.0 5 35.73642346392266 37.0 35.0 37.0 33.0 37.0 6 35.785546383391 37.0 35.0 37.0 33.0 37.0 7 35.72481944997418 37.0 35.0 37.0 33.0 37.0 8 35.698135394954335 37.0 35.0 37.0 33.0 37.0 9 37.295944265233196 39.0 37.0 39.0 34.0 39.0 10-11 37.1850528368449 39.0 37.0 39.0 33.0 39.0 12-13 37.098662469137935 39.0 37.0 39.0 33.0 39.0 14-15 38.141230242422075 40.0 37.5 41.0 33.0 41.0 16-17 38.121038469266125 40.0 37.5 41.0 33.0 41.0 18-19 38.31622848591901 40.0 38.0 41.0 33.5 41.0 20-21 38.326175840132336 40.0 38.0 41.0 34.0 41.0 22-23 38.20858936163131 40.0 37.5 41.0 33.0 41.0 24-25 38.10081711290062 40.0 37.0 41.0 33.0 41.0 26-27 37.86904252709637 40.0 37.0 41.0 33.0 41.0 28-29 37.63384615005344 39.5 37.0 41.0 32.0 41.0 30-31 37.3249579980056 39.0 36.0 41.0 32.0 41.0 32-33 37.11055368674586 39.0 35.5 41.0 31.5 41.0 34-35 36.92188491884956 39.0 35.0 41.0 31.0 41.0 36-37 36.67151235530421 39.0 35.0 40.0 30.5 41.0 38-39 36.46844026225516 39.0 35.0 40.0 30.0 41.0 40-41 36.21196492910507 38.5 35.0 40.0 30.0 41.0 42-43 35.918716433842704 38.0 35.0 40.0 30.0 41.0 44-45 35.499256721859894 38.0 35.0 40.0 28.0 41.0 46-47 34.98265807603131 38.0 34.0 40.0 25.5 41.0 48-49 34.497675869099226 38.0 34.0 40.0 24.0 41.0 50-51 34.169109952161485 38.0 33.0 40.0 23.0 41.0 52-53 33.86376069155683 38.0 33.0 40.0 22.5 41.0 54-55 33.56426089432507 38.0 33.0 40.0 21.5 41.0 56-57 33.164066631248986 37.0 32.5 40.0 19.5 41.0 58-59 32.783816381948824 37.0 32.0 39.0 18.0 40.0 60-61 32.37190038840289 36.0 31.0 39.0 14.0 40.0 62-63 31.91429251138029 36.0 31.0 39.0 10.5 40.0 64-65 31.511779047938358 35.0 30.5 39.0 8.0 40.0 66-67 30.97451591391779 35.0 29.5 38.0 7.0 40.0 68-69 30.384595283203762 34.5 29.0 38.0 2.0 40.0 70-71 29.79620681621801 34.0 28.0 37.0 2.0 39.0 72-73 29.181627964021885 34.0 27.5 36.5 2.0 39.0 74-75 28.52868400324922 33.5 26.0 36.0 2.0 39.0 76-77 26.761267813098215 31.0 25.0 34.0 2.0 36.0 78-79 27.68956427630225 32.5 26.0 35.0 2.0 37.0 80-81 27.596252053875954 33.0 26.0 35.0 2.0 37.0 82-83 27.08196521261083 33.0 25.5 35.0 2.0 36.5 84-85 26.40172765943809 32.0 24.5 35.0 2.0 36.0 86-87 25.563192200879854 31.5 21.0 34.0 2.0 35.5 88-89 24.727090577624494 31.0 18.5 34.0 2.0 35.0 90-91 23.948640466625825 31.0 11.5 34.0 2.0 35.0 92-93 23.3056561864038 30.0 4.5 34.0 2.0 35.0 94-95 22.43565097236312 29.5 2.0 34.0 2.0 35.0 96-97 21.269645408254455 29.0 2.0 34.0 2.0 35.0 98-99 19.823366051541345 28.0 2.0 34.0 2.0 35.0 100-101 15.878157853540925 13.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3301907555213752 1101 2 0.29272284359255707 1101 3 0.2264942100620857 1101 4 0.25878053346155383 1101 5 0.26697979195326127 1101 6 0.17104607737388733 1101 7 0.27266956185050617 1101 8 0.24345550628839874 1101 9 0.317633699294106 1101 10-11 0.3757893278201223 1101 12-13 0.22728058852031552 1101 14-15 0.3830468781501679 1101 16-17 0.2333126649473698 1101 18-19 0.29809810341936327 1101 20-21 0.4843598164392162 1101 22-23 0.31585091104798835 1101 24-25 0.45379507281208475 1101 26-27 0.5761404026161898 1101 28-29 0.4245637600591792 1101 30-31 0.3434836610249974 1101 32-33 0.5894160651430482 1101 34-35 0.54405643883198 1101 36-37 0.4368787524786768 1101 38-39 0.5720759120250278 1101 40-41 0.5847268073238254 1101 42-43 0.7270237362073217 1101 44-45 0.7982862515548135 1101 46-47 0.7650249658339661 1101 48-49 0.64889249007323 1101 50-51 0.6232981290993891 1101 52-53 0.5386954486477222 1101 54-55 0.514922445473907 1101 56-57 0.5978153408596754 1101 58-59 0.6899234336883566 1101 60-61 0.87082648812315 1101 62-63 0.8396035921017351 1101 64-65 0.728958181452807 1101 66-67 0.9465826952308127 1101 68-69 1.1215572004900949 1101 70-71 0.7648428689505486 1101 72-73 0.9929014108514309 1101 74-75 1.044396439696321 1101 76-77 1.1838766378524603 1101 78-79 1.0929579720240667 1101 80-81 1.3248466142943052 1101 82-83 1.4532785937021941 1101 84-85 1.284852413004998 1101 86-87 0.9728340331963388 1101 88-89 1.0228855772794638 1101 90-91 0.9881243403988407 1101 92-93 0.8670206249968331 1101 94-95 0.8663130043054679 1101 96-97 0.8826656573234963 1101 98-99 0.8483362281470761 1101 100-101 0.7374843959753932 1102 1 0.27506803075579356 1102 2 0.4312133749402278 1102 3 0.37128185484973386 1102 4 0.2440030008268792 1102 5 0.35079575076921543 1102 6 0.3174309826873625 1102 7 0.2687855758236637 1102 8 0.34354283851858725 1102 9 0.3284927726531848 1102 10-11 0.35264628681994026 1102 12-13 0.37377465912152275 1102 14-15 0.5219338518585772 1102 16-17 0.5513743490804828 1102 18-19 0.5006571675855724 1102 20-21 0.7040980495703053 1102 22-23 0.5833690669590013 1102 24-25 0.549136587796454 1102 26-27 0.6131876539491614 1102 28-29 0.618349382416234 1102 30-31 0.6959496474195603 1102 32-33 0.7665683449739049 1102 34-35 0.9107828591298315 1102 36-37 0.7804161674446704 1102 38-39 0.7933711868917328 1102 40-41 0.8969912401239739 1102 42-43 1.304531090500383 1102 44-45 1.4009763140663054 1102 46-47 1.2615776409580732 1102 48-49 1.0038290592955121 1102 50-51 0.9972869138024549 1102 52-53 0.9934608007345034 1102 54-55 0.8876242288899832 1102 56-57 0.8924811801683745 1102 58-59 1.1928881330816168 1102 60-61 1.2193223053689621 1102 62-63 1.3961052468176796 1102 64-65 1.6561104612836566 1102 66-67 1.1906073321125952 1102 68-69 1.1642235913707708 1102 70-71 0.6638649777940877 1102 72-73 0.9403814163671491 1102 74-75 0.8797751857893488 1102 76-77 1.2115471655229868 1102 78-79 1.1415882349400448 1102 80-81 1.2616628948605886 1102 82-83 1.210454774985518 1102 84-85 0.9366266362077944 1102 86-87 0.8704025200505363 1102 88-89 1.0027072356725526 1102 90-91 1.2003703874663145 1102 92-93 1.2462780712185655 1102 94-95 1.0819948851659227 1102 96-97 0.9327035778971329 1102 98-99 0.30933549271776783 1102 100-101 0.23565156824970757 1103 1 0.05790411359187786 1103 2 0.1280660717929294 1103 3 0.10961109317896955 1103 4 -0.02169256486868676 1103 5 0.09847329844676267 1103 6 0.09375015900653949 1103 7 0.12197382901191389 1103 8 0.038956433932185064 1103 9 0.07331551747592613 1103 10-11 0.024537008710659336 1103 12-13 0.18526992061678982 1103 14-15 0.15100838093310642 1103 16-17 0.20508910279523462 1103 18-19 0.08871730564570868 1103 20-21 0.15055879603104927 1103 22-23 0.27588105947099706 1103 24-25 0.3949128835727507 1103 26-27 0.22688251764402168 1103 28-29 0.11306097712782304 1103 30-31 0.2788500803199909 1103 32-33 0.40798053638609844 1103 34-35 0.23872248706945953 1103 36-37 0.3821477691762709 1103 38-39 0.4738848174053629 1103 40-41 0.3207773639101035 1103 42-43 0.20942169539099353 1103 44-45 0.45228671537670806 1103 46-47 0.7554702348506694 1103 48-49 0.484050189516644 1103 50-51 0.5845690010845388 1103 52-53 0.3889621462358477 1103 54-55 0.3411283823941389 1103 56-57 0.5243227620099518 1103 58-59 0.6244781646716504 1103 60-61 0.5579206439673001 1103 62-63 0.5750627757752049 1103 64-65 0.29232784750104557 1103 66-67 0.40400384550910573 1103 68-69 0.4583150854622602 1103 70-71 0.3214407353698441 1103 72-73 0.3343091102948428 1103 74-75 0.1478751038892696 1103 76-77 0.2260844300602507 1103 78-79 0.39240138575319605 1103 80-81 0.7362914194891097 1103 82-83 0.8080211725519142 1103 84-85 0.6353162348973918 1103 86-87 0.6226021222501394 1103 88-89 0.5386555216208393 1103 90-91 0.4456962327921623 1103 92-93 0.4668466917871861 1103 94-95 0.16653331970435303 1103 96-97 0.0018330970266511315 1103 98-99 -0.4498548664725881 1103 100-101 -0.6194613368631998 1104 1 0.09668287868643333 1104 2 -7.7876757822537E-4 1104 3 0.0773714030445447 1104 4 -0.004422116019291877 1104 5 0.24536325059987263 1104 6 -0.008756106657621388 1104 7 0.05466920381255136 1104 8 0.10619270643161371 1104 9 0.01578532836678903 1104 10-11 0.09629595152223658 1104 12-13 0.07107534326431164 1104 14-15 0.12699379902378638 1104 16-17 -0.04584505077049528 1104 18-19 0.027701128840057265 1104 20-21 0.07461894640699285 1104 22-23 0.22346593074006904 1104 24-25 0.051964603782366225 1104 26-27 0.15562482796527632 1104 28-29 0.14117672103304812 1104 30-31 0.17715265493835375 1104 32-33 0.1867911994072884 1104 34-35 0.25158418677326466 1104 36-37 0.48843621756998346 1104 38-39 0.388626216357288 1104 40-41 0.46472814996614886 1104 42-43 0.36806831193234757 1104 44-45 0.6283860546339426 1104 46-47 0.2435426887125871 1104 48-49 0.48902854449499245 1104 50-51 0.42570593432674286 1104 52-53 0.7401924948346235 1104 54-55 0.5391347619794615 1104 56-57 0.4105838693236876 1104 58-59 0.4172327784788976 1104 60-61 0.5317757362434463 1104 62-63 0.6531329512138022 1104 64-65 0.5926240425340801 1104 66-67 0.6261387281703072 1104 68-69 0.5954875621084241 1104 70-71 0.40446648155348797 1104 72-73 0.19229498407019108 1104 74-75 0.3417917135953523 1104 76-77 0.6030495701832841 1104 78-79 0.8528935251927052 1104 80-81 0.9447557621639824 1104 82-83 0.9336648271429375 1104 84-85 0.8589662690737399 1104 86-87 0.659774598896302 1104 88-89 1.1022804931405474 1104 90-91 1.0033745194178145 1104 92-93 1.3408726658131584 1104 94-95 0.9105866348103007 1104 96-97 0.6812133658806054 1104 98-99 0.6655130988953744 1104 100-101 0.1288981983384403 1105 1 -0.08202303243282927 1105 2 -0.11631150185293748 1105 3 -0.01479498854418182 1105 4 0.038459478210185694 1105 5 0.02107386860831184 1105 6 0.0019383123166463179 1105 7 0.07522058225866601 1105 8 -0.14777147230791599 1105 9 -0.13336804286617365 1105 10-11 -1.9532309295300365E-5 1105 12-13 -0.017184941594166503 1105 14-15 0.023735653660381217 1105 16-17 0.07289081327987645 1105 18-19 0.07423361860104905 1105 20-21 0.1386249981216423 1105 22-23 0.17821713802658223 1105 24-25 0.10686251930287227 1105 26-27 -0.00483271833950738 1105 28-29 0.02037013138820498 1105 30-31 -0.04252305873607298 1105 32-33 -0.055671665070981646 1105 34-35 0.27122169517841854 1105 36-37 0.09629886198589332 1105 38-39 0.21134918230875144 1105 40-41 0.33478591633328847 1105 42-43 0.2748398138578878 1105 44-45 0.013191054939582614 1105 46-47 -0.2146913109206352 1105 48-49 0.2530238987342557 1105 50-51 0.3400726437833086 1105 52-53 0.3281211579558416 1105 54-55 0.380738772004527 1105 56-57 0.231283484214579 1105 58-59 0.10578953622692921 1105 60-61 -0.11778413905455665 1105 62-63 0.025236991803083697 1105 64-65 -0.05855115154868784 1105 66-67 -0.05461192896032685 1105 68-69 -0.11780939980856431 1105 70-71 -0.2300084252256518 1105 72-73 -0.21457432334468862 1105 74-75 -0.0221043069438025 1105 76-77 0.02969545318346789 1105 78-79 0.12627056535408343 1105 80-81 0.21820462823158948 1105 82-83 0.49086881271663074 1105 84-85 0.35272199986413355 1105 86-87 0.08855080771101953 1105 88-89 0.2809741785736435 1105 90-91 0.402767819741797 1105 92-93 0.44069844978528394 1105 94-95 0.043481371652408285 1105 96-97 -0.3222403904614701 1105 98-99 -0.16282604163888692 1105 100-101 0.036921304885295214 1106 1 0.038561310895190104 1106 2 0.0990654792904877 1106 3 0.07033778941737978 1106 4 0.07875901807364727 1106 5 0.10978849373236699 1106 6 0.08596435664613722 1106 7 0.18767434304781716 1106 8 0.031242692679597894 1106 9 0.1288345976559171 1106 10-11 0.15076164720410645 1106 12-13 0.09291400279848006 1106 14-15 0.19673195452351422 1106 16-17 -0.006094781138337169 1106 18-19 0.17056831530189953 1106 20-21 0.034758197123181844 1106 22-23 0.19021001042140284 1106 24-25 0.3172494249598472 1106 26-27 -0.04676153251050863 1106 28-29 0.03466970132594582 1106 30-31 0.14833059980050933 1106 32-33 0.044589981195294115 1106 34-35 0.1719314482685519 1106 36-37 0.1470123743614664 1106 38-39 0.19757290490355928 1106 40-41 0.14919826483409082 1106 42-43 -0.07534138370007781 1106 44-45 -5.220664702960676E-4 1106 46-47 -0.3034405214706979 1106 48-49 -0.5353975021258677 1106 50-51 -0.3829394460786517 1106 52-53 -0.3454021947252883 1106 54-55 -0.5187063574652697 1106 56-57 -0.21604667126946708 1106 58-59 0.09529661872068829 1106 60-61 0.1071143602386968 1106 62-63 0.08271646610461048 1106 64-65 -0.2628029033757002 1106 66-67 0.10196531683679311 1106 68-69 0.059799544283343664 1106 70-71 0.14081876794146808 1106 72-73 0.22890933128224233 1106 74-75 -0.01944198099871386 1106 76-77 0.1788563806743717 1106 78-79 0.15228256499319315 1106 80-81 -0.16801383136360926 1106 82-83 -0.4298989837406175 1106 84-85 -0.37483957723129535 1106 86-87 0.06027647009711501 1106 88-89 0.21582576198466796 1106 90-91 0.17462956826063802 1106 92-93 0.2763139199967064 1106 94-95 0.1856207058077679 1106 96-97 5.544127023426881E-4 1106 98-99 0.1657039273846017 1106 100-101 0.20330276709695738 1107 1 0.05000368069144656 1107 2 0.045273864000719755 1107 3 0.0479227814906622 1107 4 0.03718189400577643 1107 5 0.11102741100087599 1107 6 0.14970253995892335 1107 7 0.057363439401527216 1107 8 0.13376162873737485 1107 9 0.1896575088179162 1107 10-11 0.22442878360244123 1107 12-13 0.2622720851189513 1107 14-15 0.12790232032704552 1107 16-17 0.1969181071242403 1107 18-19 0.18785150477990697 1107 20-21 0.3428207007929558 1107 22-23 0.1516927477826826 1107 24-25 0.23100595716582717 1107 26-27 0.2698478856093871 1107 28-29 0.12447872604557375 1107 30-31 0.25844964741956034 1107 32-33 0.18979871820427974 1107 34-35 0.277304738151706 1107 36-37 0.12743997696847487 1107 38-39 0.2035384970590428 1107 40-41 0.3198033379360723 1107 42-43 0.11477883824813517 1107 44-45 0.012189312779305794 1107 46-47 0.09053516991560429 1107 48-49 0.2574809254473749 1107 50-51 0.4385192175347612 1107 52-53 0.5692651765388845 1107 54-55 0.46228639105213887 1107 56-57 0.4649071775943696 1107 58-59 0.4298894200829082 1107 60-61 0.5550526785993313 1107 62-63 0.5810151567275916 1107 64-65 0.6704122630854634 1107 66-67 0.5223480013532615 1107 68-69 0.8099925746397503 1107 70-71 0.6251745016036132 1107 72-73 0.6601207986065312 1107 74-75 0.37926038527454864 1107 76-77 0.3172099711857932 1107 78-79 0.5646416454934524 1107 80-81 0.7935966142943052 1107 82-83 0.9311272331579765 1107 84-85 0.7551755422566977 1107 86-87 1.0403510399990594 1107 88-89 1.4097053051706219 1107 90-91 1.4788131159090447 1107 92-93 1.0503964753369708 1107 94-95 1.2524099430809805 1107 96-97 1.009408854602409 1107 98-99 1.213727384609662 1107 100-101 0.931404464002604 1108 1 0.12874309056582334 1108 2 0.07487509099458833 1108 3 0.004320678072602391 1108 4 0.14921811953892927 1108 5 -0.061306798756646685 1108 6 -0.082761672720018 1108 7 0.08380953931388291 1108 8 0.05207889429390633 1108 9 0.15241678430725614 1108 10-11 0.0809508402778718 1108 12-13 0.05942735827115797 1108 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2316 66-67 -0.04844428107695009 2316 68-69 -0.04380463389883715 2316 70-71 -0.14623354437339842 2316 72-73 0.015149534238716456 2316 74-75 -0.10702861308341483 2316 76-77 0.10013279548792653 2316 78-79 -0.13513257453938365 2316 80-81 -0.1081480490882889 2316 82-83 0.20442362067850084 2316 84-85 0.12559836655883672 2316 86-87 -0.1490578269960139 2316 88-89 -0.592490918146293 2316 90-91 -0.6122377872108267 2316 92-93 -0.13244424715892578 2316 94-95 0.01675723372137483 2316 96-97 0.01162560337088081 2316 98-99 0.4963833780415179 2316 100-101 0.5230300797661478 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 14.0 5 37.0 6 121.0 7 231.0 8 465.0 9 877.0 10 1425.0 11 2179.0 12 2968.0 13 4045.0 14 5292.0 15 7163.0 16 9734.0 17 13443.0 18 12014.0 19 8239.0 20 7826.0 21 8468.0 22 9227.0 23 10436.0 24 11671.0 25 13361.0 26 15427.0 27 18271.0 28 21430.0 29 25264.0 30 30394.0 31 35871.0 32 42778.0 33 53853.0 34 69066.0 35 92038.0 36 112513.0 37 118965.0 38 45140.0 39 1023.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.257643540374204 23.9086482612027 17.254504560172027 19.57920363825107 2 15.358739558051502 24.27869355616064 39.74837015004851 20.614196735739352 3 18.407782356327292 28.701629911583183 32.22018289819309 20.67040483389644 4 12.350250409542557 20.875145296701103 39.24903022541173 27.52557406834461 5 11.437361867982462 40.239574692057275 35.196623569682636 13.126439870277625 6 26.718198974202206 40.910990285613565 18.036882866514397 14.333927873669833 7 24.59658979551839 36.12368739915515 22.571372574639053 16.7083502306874 8 24.317398255330218 36.02741870472382 20.910152094676132 18.745030945269832 9 25.330253392516187 18.227694568152934 22.47892504477542 33.96312699455546 10-11 23.175054944648583 29.081034574143533 28.01480639638296 19.72910408482492 12-13 24.762502295780326 27.47084513066534 28.37812518874704 19.388527384807297 14-15 21.95429360319994 28.552815664425314 28.025429421952136 21.46746131042261 16-17 18.93456515441933 31.442130499898308 29.227564359364695 20.395739986317665 18-19 18.641304816762833 29.63695238705685 33.018683029468576 18.703059766711743 20-21 19.99097712108531 28.496396395285917 32.831445965651426 18.681180517977346 22-23 19.725036393510923 28.34909666436986 32.80167786103534 19.124189081083877 24-25 19.39530687033063 29.005350863028507 33.048759292517545 18.55058297412332 26-27 19.23919380823424 28.780395207027 33.454603948618896 18.525807036119865 28-29 18.73153360590974 29.007261445312356 33.55734397014068 18.703860978637223 30-31 19.195003888959423 28.894413827192146 33.16166854232433 18.748913741524103 32-33 19.107425262335273 29.28589830032135 32.81166219426061 18.79501424308277 34-35 19.48270623307803 28.83722306213602 33.00826306716523 18.671807637620716 36-37 19.62634564014442 28.521962301736497 32.83448241006569 19.01720964805339 38-39 18.98331221371411 29.20461213038612 32.96959514429548 18.842480511604286 40-41 19.01201544534806 28.919041352977974 33.168977408250875 18.899965793423092 42-43 19.070284862006485 29.01863744499365 32.38151293650696 19.529564756492906 44-45 19.345291656702766 28.99137393345183 32.17468585980854 19.488648550036856 46-47 19.835061178731383 29.389827437334404 31.381518248229167 19.393593135705046 48-49 19.643385416409867 29.392568177223804 31.302417943269056 19.661628463097276 50-51 19.266681540447028 29.61581271856137 31.242334558982137 19.875171182009463 52-53 19.51550068688557 30.173413183395674 30.785046417933355 19.526039711785405 54-55 19.539155226798442 29.62579705178664 30.8697710136317 19.965276707783218 56-57 19.76725266435423 29.827146706505637 30.52654739629172 19.879053232848413 58-59 19.852068799533562 29.38617981634639 30.93643124761558 19.82532013650447 60-61 19.73752167905264 30.003808857010085 30.714672234911568 19.54399722902571 62-63 19.591598757982943 29.66277190694487 31.14071796869318 19.604911366379007 64-65 19.474010534087867 29.634117329474368 30.857629571376272 20.03424256506149 66-67 19.436745586381804 29.645647089276967 30.39016107510778 20.527446249233453 68-69 19.356804979892452 29.41963914269426 30.75934115036748 20.464214727045807 70-71 19.732185669153708 29.425617333788583 30.783425020739063 20.05877197631864 72-73 19.81877818879265 28.922456244584115 30.756985027198063 20.501780539425173 74-75 19.35117858274239 29.872015639656784 30.114536326332384 20.662269451268443 76-77 19.47639095236183 29.289180331919706 30.65305958554824 20.58136913017022 78-79 19.286107408071658 29.25744249297861 30.702644802196062 20.753805296753672 80-81 19.422325489664352 29.572645358511963 30.276295191489886 20.7287339603338 82-83 19.54254496956011 29.36312280360077 30.406855415761196 20.687476811077925 84-85 19.090291653467215 29.176132266529926 30.376532625965925 21.357043454036937 86-87 19.200031091669224 29.27397867876445 30.32443293175317 21.20155729781316 88-89 19.58348123018852 28.879834372012432 30.180852603211395 21.355831794587655 90-91 19.369802127926423 28.50557683289766 30.59630995489231 21.528311084283605 92-93 19.679860367250892 29.02273099864287 30.30830634892656 20.989102285179676 94-95 19.449542754517292 28.465580551998038 30.41203247743356 21.67284421605111 96-97 19.356910329593934 28.436243869187976 30.20630590764368 22.000539893574402 98-99 19.293503215018912 28.520450330685016 29.919428846482155 22.266617607813917 100-101 19.39637665751432 27.75989423956956 30.925742742067197 21.917986360848918 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2664.0 1 1664.5 2 724.0 3 780.0 4 713.0 5 773.5 6 1349.0 7 2964.5 8 4269.5 9 4249.0 10 3349.5 11 2374.0 12 2143.5 13 2137.5 14 2051.0 15 1996.5 16 1995.5 17 2029.5 18 2116.5 19 2223.0 20 2431.5 21 2788.0 22 3113.5 23 3456.5 24 3849.5 25 4599.0 26 6124.5 27 7955.0 28 9046.5 29 10324.5 30 12796.0 31 15372.0 32 18968.0 33 23605.5 34 27620.0 35 30548.0 36 34223.5 37 37332.0 38 36684.5 39 34950.0 40 33420.0 41 31749.0 42 29689.0 43 28704.5 44 28876.0 45 28022.5 46 26322.5 47 26348.5 48 30206.0 49 28903.5 50 22540.5 51 18211.0 52 15046.0 53 13462.0 54 12369.5 55 10928.0 56 9585.0 57 8640.5 58 7706.0 59 6620.0 60 5667.5 61 4720.5 62 3776.5 63 2875.5 64 2172.0 65 1677.5 66 1278.5 67 1074.0 68 887.5 69 665.0 70 475.5 71 368.5 72 318.5 73 245.5 74 184.0 75 157.5 76 124.0 77 77.0 78 45.5 79 33.5 80 27.0 81 20.0 82 12.5 83 6.5 84 4.0 85 2.0 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2577437132598108 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 7.395802389090698E-4 16-17 7.395802389090698E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0012942654180908722 36-37 0.004930534926060466 38-39 0.002342004089878721 40-41 0.0028966892690605235 42-43 1.2326337315151165E-4 44-45 0.0011093703583636048 46-47 6.163168657575582E-5 48-49 3.697901194545349E-4 50-51 0.0 52-53 6.163168657575582E-5 54-55 0.0 56-57 6.163168657575582E-4 58-59 0.0019105822838484303 60-61 4.930534926060466E-4 62-63 0.0012326337315151164 64-65 0.0 66-67 4.3142180603029077E-4 68-69 0.001047738671787849 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.23278288019662974 78-79 6.163168657575582E-5 80-81 1.2326337315151165E-4 82-83 0.0 84-85 0.0 86-87 0.09423484877433065 88-89 0.3342902679868996 90-91 0.24473942739232638 92-93 0.0 94-95 0.0 96-97 0.0 98-99 6.163168657575582E-5 100-101 4.3142180603029077E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 811271.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.78952929839066 #Duplication Level Percentage of deduplicated Percentage of total 1 81.06219507096269 37.92861951264668 2 10.38052979588196 9.714002060344724 3 3.1327712326797035 4.397426741298673 4 1.3813953082170027 2.585393449859154 5 0.8003022959295 1.8722883861481328 6 0.5199161000948197 1.459597775885495 7 0.3576237046537173 1.1713131364685883 8 0.2559527765148048 0.9580727948595118 9 0.22227418818374398 0.936009417626936 >10 1.5211905649073119 14.717248887041917 >50 0.21987771377853738 7.2184395193736135 >100 0.13834392171399376 11.483505299955322 >500 0.005260225160194097 1.6818846587782699 >1k 0.001578067548058229 1.479201990086691 >5k 7.890337740291145E-4 2.3969963696263368 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7789 0.9600984134771241 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6472 0.7977605510365834 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5217 0.6430650177314362 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3747 0.4618678591987141 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2848 0.35105408673550514 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1709 0.2106571047159334 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1391 0.17145935205375268 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1285 0.15839343449969245 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1040 0.1281939080775721 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 993 0.12240052953945105 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 930 0.11463493703090583 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 889 0.10958113873169384 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 859 0.1058832375371485 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 6.163168657575582E-5 0.0 20-21 0.0 0.0 0.0 1.2326337315151165E-4 0.0 22-23 0.0 0.0 0.0 1.8489505972726748E-4 0.0 24-25 0.0 0.0 0.0 5.546851791818024E-4 0.0 26-27 0.0 0.0 0.0 0.0014791604778181399 0.0 28-29 0.0 0.0 0.0 0.0036979011945453494 0.0 30-31 0.0 0.0 0.0 0.011586757076242094 0.0 32-33 0.0 0.0 0.0 0.02945994618321128 0.0 34-35 0.0 0.0 0.0 0.054790569365846926 0.0 36-37 0.0 0.0 0.0 0.09324874178911856 0.0 38-39 0.0 0.0 0.0 0.15161394897635933 0.0 40-41 0.0 0.0 0.0 0.2160806931345999 0.0 42-43 0.0 0.0 0.0 0.28282780969614346 0.0 44-45 0.0 0.0 0.0 0.3634420557372321 0.0 46-47 0.0 0.0 0.0 0.4646412850946231 0.0 48-49 0.0 0.0 0.0 0.5652858292728324 0.0 50-51 0.0 0.0 0.0 0.674682072944799 0.0 52-53 0.0 0.0 0.0 0.7886390614233715 0.0 54-55 0.0 0.0 0.0 0.9264475126067615 0.0 56-57 0.0 0.0 0.0 1.0917436960029385 0.0 58-59 0.0 0.0 0.0 1.2732490129685394 0.0 60-61 0.0 0.0 0.0 1.4701622515780794 0.0 62-63 0.0 0.0 0.0 1.6720676568002553 0.0 64-65 0.0 0.0 0.0 1.8909834075173402 0.0 66-67 0.0 0.0 0.0 2.1415778451343632 0.0 68-69 0.0 0.0 0.0 2.4154690602770224 0.0 70-71 0.0 0.0 0.0 2.733796721440801 0.0 72-73 0.0 0.0 0.0 3.072770997607458 0.0 74-75 0.0 0.0 0.0 3.4299882530005386 0.0 76-77 0.0 0.0 0.0 3.814816503979558 0.0 78-79 0.0 0.0 0.0 4.257948330459243 0.0 80-81 0.0 0.0 0.0 4.733067002271744 0.0 82-83 0.0 0.0 0.0 5.209788097935215 0.0 84-85 0.0 0.0 0.0 5.705121962944565 0.0 86-87 0.0 0.0 0.0 6.234907940749761 0.0 88-89 0.0 0.0 0.0 6.790702490289927 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAGCGA 35 2.0630895E-4 33.902245 10-11 CCGAGCG 75 3.0916993E-4 31.642097 9 GTCCCGA 100 4.9776037E-5 28.477886 6 ACACCGT 85 6.43468E-4 27.919497 6 TGTCCCG 120 5.411681E-6 27.686836 5 CATATAG 315 0.0 25.681509 1 GTAAGCG 85 1.13188435E-7 25.127546 94-95 GTACATG 4485 0.0 24.191042 1 GTGTACG 100 0.0016607136 23.731571 9 TACCGCG 140 1.7893615E-5 23.731571 9 GAGTACT 5775 0.0 23.628838 12-13 CCTATAC 225 2.8358045E-9 23.204203 3 AGTACTT 6015 0.0 22.370409 12-13 GTACTTT 6060 0.0 22.282614 14-15 GTATCAA 10520 0.0 22.255201 1 TACATGG 4675 0.0 22.23407 2 ACATGGG 4575 0.0 22.20134 3 GGTATCA 8070 0.0 21.405003 1 GGATATG 450 0.0 21.149479 1 CATGGGG 1780 0.0 20.265162 4 >>END_MODULE