FastQCFastQC Report
Fri 27 May 2016
SRR939244_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939244_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences825915
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79850.9668065115659602No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57930.701403897495505No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46360.5613168425322218No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39470.4778942142956599No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21930.2655236919053413No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT18480.22375183886961733No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15060.1823432193385518No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13090.15849088586597895No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13070.15824873019620664No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT12590.1524369941216711No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA10520.12737388230023672No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC9690.11732442200468572No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8380.10146322563459921No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCCGC1553.8198777E-1133.646717
CGTGGAC650.005875836629.1973953
GTGGTAC2104.1836756E-1127.1513751
GTGTAGC3500.025.7938061
GGTGTAC1151.2785954E-424.7903861
GTACACG1151.2785954E-424.7903861
GTATCAA90600.024.3867911
AGTACTT48800.023.60848212-13
GAGTACT47200.023.20339812-13
GTACATG49200.023.1780031
GTTACAC1252.2726636E-422.7739683
TACATGG50300.022.5438142
CATGGGG19550.022.3274214
ACATGGG48400.022.2524593
GTACTTT49150.022.18635414-15
GGTATCA66400.021.6822551
CAACGCA104300.020.8797785
TCAACGC104350.020.8697724
ATCAACG104650.020.8099463
ATGGGCG1150.003747116320.6285955
Produced by FastQC (version 0.11.5)