FastQCFastQC Report
Fri 27 May 2016
SRR939244_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939244_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences825915
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81030.9810936960825266No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67010.8113425715721351No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54860.664233002185455No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38280.46348595194420733No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29050.35173111034428484No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17290.2093435765181647No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14520.17580501625469933No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12670.15340561680076037No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT10860.13149052868636604No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA10000.12107783488615657No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9630.11659795499536878No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA9320.11284454211389792No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT9160.1109072967557194No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC8290.10037352512062378No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCG250.001623142237.96664-65
TCGCGGA452.3569683E-531.77684482-83
GGATATG3800.027.5537971
CGCGGAA559.2578564E-525.99923782-83
CGCGCTT559.536943E-525.8859162-63
GATTGGC3550.025.399799
GTATAGG3700.024.4396061
GAGTACT59350.023.6687812-13
AGTACTT61250.023.2057512-13
GTACTTT62150.022.56426614-15
CGGGTAA550.002892878621.57159254-55
GTATCAA112300.021.5291231
GGTATCA87150.021.1888161
CATTCCG2257.2133844E-821.0922229
GTACATG45500.020.5015661
ATTCGCG704.855508E-420.35556480-81
CGATCGT600.004789097219.7739624-25
ACTCGAG858.000278E-519.54487438-39
ATATGAG5350.019.5152343
ACTTTTT73500.019.30584116-17
Produced by FastQC (version 0.11.5)