FastQCFastQC Report
Fri 27 May 2016
SRR939243_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939243_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2666485
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90370.33891058828382686No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66230.24837942084804526No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50480.1893128969411041No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30260.11348273101105012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA115150.029.507251
GTACATG95250.026.205981
GGTATCA82000.025.9843651
TACATGG96500.024.9607052
ATCAACG137900.024.171943
TCAACGC138200.024.1533244
CAACGCA139150.024.02255
ACATGGG97400.023.6590753
TATCAAC142900.023.4588992
AACGCAG142000.023.427866
GAGTACT76550.021.23225212-13
ACGCAGA158600.020.7964827
CGCAGAG161500.020.5404228
CATGGGG61200.020.065994
AGTACTT80900.019.85629812-13
GTACTTT81700.019.8100314-15
AGAGTAC127700.019.77749310-11
GCAGAGT172550.019.0317049
TACACTG21350.017.9886865
GTGTAAG9200.017.5376241
Produced by FastQC (version 0.11.5)