##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939243_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2666485 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.074507450820086 33.0 31.0 34.0 30.0 34.0 2 32.16415205785894 34.0 31.0 34.0 30.0 34.0 3 32.21968696617457 34.0 31.0 34.0 30.0 34.0 4 35.70319728031472 37.0 35.0 37.0 35.0 37.0 5 35.723837186408325 37.0 35.0 37.0 35.0 37.0 6 35.81061959846014 37.0 36.0 37.0 35.0 37.0 7 35.75189997318567 37.0 36.0 37.0 35.0 37.0 8 35.73646092140027 37.0 35.0 37.0 35.0 37.0 9 37.396669773128295 39.0 37.0 39.0 35.0 39.0 10-11 37.29558313660118 39.0 37.0 39.0 34.5 39.0 12-13 37.19964485080546 39.0 37.0 39.0 34.0 39.0 14-15 38.47397303941331 40.0 38.0 41.0 34.0 41.0 16-17 38.388423148827016 40.0 38.0 41.0 34.0 41.0 18-19 38.29446087264695 40.0 38.0 41.0 34.0 41.0 20-21 38.26211660669383 40.0 38.0 41.0 34.0 41.0 22-23 38.278950378494535 40.0 38.0 41.0 34.0 41.0 24-25 38.19719705904965 40.0 38.0 41.0 34.0 41.0 26-27 38.1044834304337 40.0 38.0 41.0 33.5 41.0 28-29 37.95168002070141 40.0 38.0 41.0 33.0 41.0 30-31 37.773280554737795 40.0 38.0 41.0 33.0 41.0 32-33 37.5546753497582 40.0 37.0 41.0 32.5 41.0 34-35 37.26859179781623 40.0 37.0 41.0 31.5 41.0 36-37 37.03423701989698 40.0 37.0 41.0 31.0 41.0 38-39 36.431264192373106 39.5 35.5 40.5 30.5 41.0 40-41 36.407252056546355 39.0 35.5 40.0 30.0 41.0 42-43 36.60596965668286 39.5 36.0 41.0 30.0 41.0 44-45 36.52153002923324 40.0 36.0 41.0 30.0 41.0 46-47 36.25808826976338 40.0 36.0 41.0 29.5 41.0 48-49 36.01352623397469 39.0 35.5 41.0 28.5 41.0 50-51 34.88894255921185 38.0 34.0 40.0 26.5 40.5 52-53 34.99880254342327 38.5 34.5 39.5 27.0 40.5 54-55 35.47316673448378 39.0 35.0 40.5 28.0 41.0 56-57 35.35930635274528 39.0 35.0 41.0 27.0 41.0 58-59 35.126716820083374 38.5 35.0 40.0 26.5 41.0 60-61 34.80232947119522 38.0 34.5 40.0 26.0 41.0 62-63 34.404279791560796 37.5 34.0 40.0 26.0 41.0 64-65 34.00091712497914 37.0 34.0 40.0 25.0 41.0 66-67 33.61934681800197 36.0 33.5 39.0 23.5 41.0 68-69 33.200912249647004 36.0 33.0 39.0 22.5 40.5 70-71 32.77757928508879 35.0 33.0 38.5 22.0 40.0 72-73 32.33211343772794 35.0 32.0 37.5 20.5 40.0 74-75 31.865582217788585 35.0 32.0 37.0 19.5 39.0 76-77 31.403730566644853 35.0 31.0 36.5 18.0 39.0 78-79 31.00675608525831 35.0 31.0 36.0 16.0 38.5 80-81 30.604060214102084 34.5 31.0 35.5 10.0 37.0 82-83 30.183648698567588 34.0 30.5 35.0 7.0 37.0 84-85 29.767281271036587 34.0 30.0 35.0 3.5 36.5 86-87 29.3479382032901 34.0 29.5 35.0 2.0 36.0 88-89 28.921046621301077 34.0 29.0 35.0 2.0 36.0 90-91 28.512337778011126 34.0 29.0 35.0 2.0 35.0 92-93 28.057822189136637 34.0 28.5 35.0 2.0 35.0 94-95 27.56385353752224 34.0 27.0 35.0 2.0 35.0 96-97 26.932756794056594 33.0 25.5 35.0 2.0 35.0 98-99 26.098584653579522 33.0 24.5 35.0 2.0 35.0 100-101 23.270088712293525 29.5 10.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3518380250414026 1101 2 0.38748061993951666 1101 3 0.3968884232212133 1101 4 0.3122716050091512 1101 5 0.30573619955747233 1101 6 0.32203469978400534 1101 7 0.3068454609373674 1101 8 0.17403868988319715 1101 9 0.3468067891509179 1101 10-11 0.4265976034214489 1101 12-13 0.4936489185511235 1101 14-15 0.5995475745011376 1101 16-17 0.6048455944657292 1101 18-19 0.6169650915008091 1101 20-21 0.6367846468420097 1101 22-23 0.6875711986825053 1101 24-25 0.632685839515041 1101 26-27 0.6165213412655248 1101 28-29 0.6957151256057159 1101 30-31 0.696632309828054 1101 32-33 0.7010772157796694 1101 34-35 0.6895538966964878 1101 36-37 0.6152356184687662 1101 38-39 1.0945012241961578 1101 40-41 0.8535153845078227 1101 42-43 0.7339731104714033 1101 44-45 0.7072346635820423 1101 46-47 0.6569208620442666 1101 48-49 0.7144534486364691 1101 50-51 0.7798799101286775 1101 52-53 0.7802522968909571 1101 54-55 0.7867083487139652 1101 56-57 0.7097149301951262 1101 58-59 0.6940368655738922 1101 60-61 0.7486874104750214 1101 62-63 0.8089621217127316 1101 64-65 0.6885474807052532 1101 66-67 0.5557879880756289 1101 68-69 0.7435417477536816 1101 70-71 0.7426462002003404 1101 72-73 0.7000413328255419 1101 74-75 0.6974509565227969 1101 76-77 0.6447297888296184 1101 78-79 0.6086088309473823 1101 80-81 0.5101406220250482 1101 82-83 0.4715753091174406 1101 84-85 0.5506173881331264 1101 86-87 0.5671376382558364 1101 88-89 0.4661303569694404 1101 90-91 0.5542294440064559 1101 92-93 0.560304931566364 1101 94-95 0.6482199490036642 1101 96-97 0.7391294023664443 1101 98-99 0.7481033557804153 1101 100-101 0.5540288477749762 1102 1 0.28533838961423186 1102 2 0.26678805222636726 1102 3 0.3625173070422818 1102 4 0.2931793654977284 1102 5 0.23293324643137225 1102 6 0.2457687357172702 1102 7 0.21984571432840738 1102 8 0.26074539922987583 1102 9 0.3298502741568967 1102 10-11 0.35414370938717354 1102 12-13 0.3428829708600034 1102 14-15 0.5261516518560754 1102 16-17 0.5186622582882023 1102 18-19 0.38384102124771147 1102 20-21 0.4978968352959967 1102 22-23 0.41414567065520913 1102 24-25 0.349940864673151 1102 26-27 0.34707853438225555 1102 28-29 0.34726667172202497 1102 30-31 0.3873894405450855 1102 32-33 0.261966049508608 1102 34-35 0.24186018613800542 1102 36-37 0.29775581649300165 1102 38-39 0.6418584159338891 1102 40-41 0.4522299420818854 1102 42-43 0.41098657811922834 1102 44-45 0.42105490418287417 1102 46-47 0.3699848079461461 1102 48-49 0.3314190564043713 1102 50-51 0.20074390464026948 1102 52-53 0.25872698622836054 1102 54-55 0.3032576281008659 1102 56-57 0.22587141036943592 1102 58-59 0.31563914161154116 1102 60-61 0.300140013421597 1102 62-63 0.37375696977290573 1102 64-65 0.294794819668347 1102 66-67 0.31506951242099035 1102 68-69 0.34722738082621163 1102 70-71 0.3287801940741346 1102 72-73 0.10371416706470882 1102 74-75 0.08724567530183336 1102 76-77 0.02817486416605064 1102 78-79 -0.012288431912214293 1102 80-81 0.04786162814263051 1102 82-83 -0.0038675218983073023 1102 84-85 0.02654179141445212 1102 86-87 -0.1069529981693833 1102 88-89 -0.040259579096723286 1102 90-91 -0.07737294407485606 1102 92-93 0.06351476693113511 1102 94-95 0.14177873141660058 1102 96-97 0.2209869483345095 1102 98-99 0.27749283850069517 1102 100-101 -0.06628061681146846 1103 1 0.27257966705892755 1103 2 0.3300248077531691 1103 3 0.36662125681210966 1103 4 0.2603720476448288 1103 5 0.19763950965185018 1103 6 0.2739135122430696 1103 7 0.2609473580472965 1103 8 0.3086923548553031 1103 9 0.36102112830914024 1103 10-11 0.36582289775435584 1103 12-13 0.30790480579207724 1103 14-15 0.5074600076920532 1103 16-17 0.45772779603628067 1103 18-19 0.49134944564664096 1103 20-21 0.5247216592493231 1103 22-23 0.5219321623649407 1103 24-25 0.511785289192332 1103 26-27 0.5344015266177564 1103 28-29 0.49422090970486465 1103 30-31 0.4530486624075323 1103 32-33 0.5578333535911142 1103 34-35 0.3901947161435473 1103 36-37 0.17291927497974768 1103 38-39 0.6832457145591064 1103 40-41 0.5187433104135479 1103 42-43 0.41268056302035205 1103 44-45 0.41373475332124343 1103 46-47 0.45742946185639966 1103 48-49 0.4012566587357398 1103 50-51 0.30865006357105784 1103 52-53 0.46534725727750725 1103 54-55 0.4778239934026871 1103 56-57 0.46361130123523964 1103 58-59 0.41249761691240394 1103 60-61 0.4325682694539523 1103 62-63 0.28913121599948965 1103 64-65 0.2488335739268024 1103 66-67 0.3542841572608211 1103 68-69 0.34786589505649346 1103 70-71 0.23810300767596715 1103 72-73 0.2426875552715302 1103 74-75 0.28913294877959217 1103 76-77 0.3027835321195731 1103 78-79 0.29720012368130355 1103 80-81 0.19159677452107715 1103 82-83 0.07461018386716489 1103 84-85 0.1858252217822951 1103 86-87 0.19428263815094837 1103 88-89 0.1887718477123208 1103 90-91 0.30857451103114286 1103 92-93 0.3498924459304611 1103 94-95 0.19568411742432446 1103 96-97 0.1572353925161103 1103 98-99 0.36059435272461826 1103 100-101 0.008081504871018552 1104 1 0.18818395588414205 1104 2 0.2150978749602288 1104 3 0.23669399512152722 1104 4 0.21026997397406433 1104 5 0.15236069100600957 1104 6 0.1416467497152425 1104 7 0.19764783385254958 1104 8 0.22719902109113832 1104 9 0.31978803912941345 1104 10-11 0.29185598254878187 1104 12-13 0.2811690340770383 1104 14-15 0.3062603391285492 1104 16-17 0.3326636032953303 1104 18-19 0.2627075967110599 1104 20-21 0.33062935326142195 1104 22-23 0.4291847592137472 1104 24-25 0.4383470625160797 1104 26-27 0.42228436058445595 1104 28-29 0.38641489930246564 1104 30-31 0.33845250254712766 1104 32-33 0.4386190669700838 1104 34-35 0.3904553819477812 1104 36-37 0.3677456862679733 1104 38-39 0.853995953154147 1104 40-41 0.73070644271926 1104 42-43 0.5655862294073586 1104 44-45 0.5010168297847457 1104 46-47 0.48067415823771853 1104 48-49 0.5272318113846381 1104 50-51 0.5204168670668352 1104 52-53 0.5498178359411696 1104 54-55 0.6738116441618587 1104 56-57 0.6655455078591288 1104 58-59 0.5014476948669113 1104 60-61 0.580975539361198 1104 62-63 0.558014412330607 1104 64-65 0.551157004940805 1104 66-67 0.5704245780882289 1104 68-69 0.7129543916019188 1104 70-71 0.6851748666169613 1104 72-73 0.5222156390908097 1104 74-75 0.5428186218830398 1104 76-77 0.6105656541671429 1104 78-79 0.48788040517152353 1104 80-81 0.5720507051940729 1104 82-83 0.48673392785324054 1104 84-85 0.6652030513117637 1104 86-87 0.552356382771741 1104 88-89 0.5035025093966645 1104 90-91 0.515465262775745 1104 92-93 0.4587255401879098 1104 94-95 0.4514794957777788 1104 96-97 0.5456381155577574 1104 98-99 0.5525615183540076 1104 100-101 0.3581305798608305 1105 1 0.09998528574021748 1105 2 0.24125342195882382 1105 3 0.2198650812267502 1105 4 0.1829018847426198 1105 5 0.19133269862650337 1105 6 0.12013341511612907 1105 7 0.16649992781917433 1105 8 0.23423112099776944 1105 9 0.22521339086762282 1105 10-11 0.24447926375228946 1105 12-13 0.3009422226326137 1105 14-15 0.21960318358687658 1105 16-17 0.20166662350287368 1105 18-19 0.24855645620340283 1105 20-21 0.2523248145925905 1105 22-23 0.3946981201755264 1105 24-25 0.2824635192957601 1105 26-27 0.2485417263543397 1105 28-29 0.3096170288446629 1105 30-31 0.2991208829737957 1105 32-33 0.2844305167229919 1105 34-35 0.2210282313785541 1105 36-37 0.22266228069440785 1105 38-39 0.6430861720736161 1105 40-41 0.5863506746669316 1105 42-43 0.27069510299530464 1105 44-45 0.2283358956827115 1105 46-47 0.21477396727669174 1105 48-49 0.2237605499116171 1105 50-51 0.09859709456539179 1105 52-53 0.029482056956759095 1105 54-55 0.2270472542850115 1105 56-57 0.27812389120532544 1105 58-59 0.3625438094872777 1105 60-61 0.3954004869654142 1105 62-63 0.3018352427039801 1105 64-65 0.38084908904667714 1105 66-67 0.4271327117267347 1105 68-69 0.3166207928657556 1105 70-71 0.2961129454912381 1105 72-73 0.2501756822694787 1105 74-75 0.27302481996927597 1105 76-77 0.4454637330302056 1105 78-79 0.43899757907169956 1105 80-81 0.5014914605796967 1105 82-83 0.34217917779231044 1105 84-85 0.4000635914266226 1105 86-87 0.41646863835270054 1105 88-89 0.26394541894833523 1105 90-91 0.365680188585884 1105 92-93 0.45367456629788094 1105 94-95 0.3371595641187781 1105 96-97 0.4881413597076971 1105 98-99 0.49634110798832154 1105 100-101 0.02044941718890314 1106 1 0.16905443936488496 1106 2 0.13958347982897124 1106 3 0.0770384272266611 1106 4 0.11309071900418388 1106 5 0.0957956804514879 1106 6 0.13834488163131908 1106 7 0.15351192733166386 1106 8 0.11324601184105632 1106 9 0.10453229584001633 1106 10-11 0.1852393562052299 1106 12-13 0.19886801652437214 1106 14-15 0.33203876251683795 1106 16-17 0.3085951939209792 1106 18-19 0.2721110900588499 1106 20-21 0.25891824854031853 1106 22-23 0.25829738629766297 1106 24-25 0.2560310890544244 1106 26-27 0.1759071059054378 1106 28-29 0.1298505497825957 1106 30-31 0.15099023660847877 1106 32-33 0.0964198818054669 1106 34-35 0.08515942020846268 1106 36-37 0.10097372228995738 1106 38-39 0.4867146344340867 1106 40-41 0.4043620359537883 1106 42-43 0.3061437625582428 1106 44-45 0.24024706044177435 1106 46-47 0.11954252750930294 1106 48-49 0.058010686479931906 1106 50-51 -0.012790621794728452 1106 52-53 0.0766579085227761 1106 54-55 0.1238118094205305 1106 56-57 0.23244083742896748 1106 58-59 0.2479727183336422 1106 60-61 0.17933308859726793 1106 62-63 0.1293253156564944 1106 64-65 0.14782960439346482 1106 66-67 -0.01124818111446757 1106 68-69 -0.0616808549860437 1106 70-71 0.007165947056058997 1106 72-73 0.0013861617929364911 1106 74-75 0.02477361533879119 1106 76-77 0.04165210317713175 1106 78-79 0.15770309915213687 1106 80-81 0.10946320073209037 1106 82-83 0.09192147990972188 1106 84-85 0.06620608876598766 1106 86-87 0.21312868608087854 1106 88-89 0.14000933237599256 1106 90-91 0.12870573175779398 1106 92-93 0.16142245139333156 1106 94-95 0.24253071164084972 1106 96-97 0.21907212446850366 1106 98-99 0.3307682800774572 1106 100-101 0.026423804916689164 1107 1 0.10383006513817605 1107 2 -0.01448100628081761 1107 3 0.11438218218366814 1107 4 0.023391523758689914 1107 5 0.04621431670457099 1107 6 0.0884876435995352 1107 7 0.12437169806651838 1107 8 0.09917417613364421 1107 9 0.09369838088385052 1107 10-11 0.1959774355808861 1107 12-13 0.26722297565214603 1107 14-15 0.2669748633173654 1107 16-17 0.2274658806589258 1107 18-19 0.26289197158757105 1107 20-21 0.27741682885184815 1107 22-23 0.1196786876523177 1107 24-25 0.3037987809635112 1107 26-27 0.29454084743224485 1107 28-29 0.2989361065014009 1107 30-31 0.2995470188594638 1107 32-33 0.35289585549497104 1107 34-35 0.4603130876047956 1107 36-37 0.2615794163775007 1107 38-39 0.8882244744349279 1107 40-41 0.5221676919714682 1107 42-43 0.18260806413497477 1107 44-45 0.3171054114881642 1107 46-47 0.38935306387130453 1107 48-49 0.40825073808574075 1107 50-51 0.2842373516198151 1107 52-53 0.441359417040907 1107 54-55 0.4871810711825262 1107 56-57 0.3828208750758293 1107 58-59 0.4294376544080052 1107 60-61 0.568360509470125 1107 62-63 0.6553651647518066 1107 64-65 0.5378463789247334 1107 66-67 0.5287861081952983 1107 68-69 0.5702422412566719 1107 70-71 0.49222247022051846 1107 72-73 0.46635700133905544 1107 74-75 0.5894525198786233 1107 76-77 0.5055099942127406 1107 78-79 0.6171132939429143 1107 80-81 0.5809137812974257 1107 82-83 0.5581957494443159 1107 84-85 0.6165461398430452 1107 86-87 0.8163108900084168 1107 88-89 0.7061976803556469 1107 90-91 0.5868615379399174 1107 92-93 0.6573064123505752 1107 94-95 0.6891723024321728 1107 96-97 0.6640905248999616 1107 98-99 0.7888305639516986 1107 100-101 0.43360572332029435 1108 1 0.08550133260013837 1108 2 0.17200382284976 1108 3 0.20778936334267684 1108 4 0.15847492256111195 1108 5 0.16859612459181506 1108 6 0.20129274424559895 1108 7 0.2473227115149399 1108 8 0.29044200380725016 1108 9 0.35876897321420387 1108 10-11 0.3924322436242136 1108 12-13 0.3562701440895779 1108 14-15 0.4738955827002016 1108 16-17 0.45316195910950796 1108 18-19 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-1.4412402266580315 2316 70-71 -1.2083582201243992 2316 72-73 -1.0371031325291256 2316 74-75 -0.9688266826639342 2316 76-77 -0.9043600084384344 2316 78-79 -0.9605468821643015 2316 80-81 -0.8337335324480399 2316 82-83 -0.8209460022941926 2316 84-85 -0.8430589701806355 2316 86-87 -0.7754235484724887 2316 88-89 -0.8091065850800554 2316 90-91 -0.7459241002770298 2316 92-93 -0.8788055814149978 2316 94-95 -0.9132160000531861 2316 96-97 -0.7761472204860027 2316 98-99 -0.9476102908172983 2316 100-101 -0.31703893540055716 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9908.0 3 2359.0 4 2406.0 5 2568.0 6 3074.0 7 3564.0 8 4298.0 9 5303.0 10 5998.0 11 7446.0 12 9248.0 13 12380.0 14 12788.0 15 12935.0 16 16335.0 17 20524.0 18 18436.0 19 14083.0 20 13838.0 21 14912.0 22 15817.0 23 17857.0 24 20053.0 25 23384.0 26 26642.0 27 31725.0 28 37602.0 29 46156.0 30 56597.0 31 69784.0 32 89658.0 33 119039.0 34 165431.0 35 245124.0 36 384644.0 37 577641.0 38 482365.0 39 64551.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.3732642642925 18.50258068761928 14.478926734795566 23.64522831329266 2 15.478096951105211 19.601417140092284 40.26760521581484 24.652880692987665 3 19.602145621734806 23.420282428974833 28.999815550761294 27.977756398529063 4 13.11165518034317 16.00476586611839 36.1614788463027 34.72210010723574 5 14.563814227971628 36.626985856806364 33.47772398149916 15.331475933722851 6 33.11384178288206 35.002395135846996 17.091959701651668 14.79180337961927 7 29.644145150480323 31.776007629618086 20.074493908883888 18.505353311017704 8 26.510920716842435 34.83333909122452 19.365590541837587 19.290149650095458 9 26.52752293922504 16.978518956358204 19.910278470212617 36.58367963420414 10-11 25.619300498849796 25.93793655928251 26.748682472181766 21.694080469685925 12-13 26.219723498428564 23.22784403788559 27.353741872045468 23.198690591640375 14-15 23.080109052806048 24.77767094793369 25.576153626197307 26.56606637306296 16-17 22.911178965218397 28.01225653657862 26.06755248249489 23.009012015708098 18-19 22.80229493096037 26.54380896429008 28.386167552791502 22.267728551958047 20-21 23.46669070517335 25.791721363659413 28.214746497044953 22.526841434122286 22-23 23.101693683352337 25.527645627826885 28.260365337984496 23.11029535083629 24-25 23.016555535017368 25.79433957443867 28.284000348648313 22.905104541895653 26-27 22.742887987743828 26.062437445263136 28.293306753498136 22.901367813494904 28-29 22.64459699376708 26.3119284526106 28.44661939186071 22.59685516176161 30-31 22.81051015968206 25.871494543024166 28.179749217796218 23.138246079497556 32-33 22.647039545410237 25.961618822444095 28.179802300181215 23.211539331964453 34-35 23.174871228138695 26.17217293602854 27.851371553431335 22.801584282401425 36-37 23.22471242198401 25.809731392941416 27.999905069871588 22.965651115202984 38-39 22.772526296008834 26.05055054885422 28.18526188305445 22.99166127208249 40-41 22.98371366904091 26.039114004316716 28.01245536057211 22.96471696607027 42-43 22.952665186739715 25.902023512609386 27.80013528751988 23.345176013131024 44-45 22.914220801501774 26.18843152387334 27.723747462797427 23.173600211827463 46-47 23.031516453168273 26.493248895720704 27.42313361073246 23.052101040378563 48-49 23.189266759951472 26.237528933326725 27.45460747394753 23.118596832774273 50-51 23.022130680705267 26.617142086563987 27.2858614060094 23.07486582672135 52-53 23.228076387724776 26.578185537262467 27.044675028205596 23.149063046807164 54-55 22.944200100374097 26.34507408945347 27.25616203731167 23.454563772860766 56-57 23.01250425442837 26.575324466050414 27.323727361170096 23.08844391835112 58-59 23.10929716107994 26.24482979693389 27.331066544141592 23.314806497844582 60-61 23.145622460732966 26.382845740127316 27.515221961618945 22.956309837520774 62-63 22.862208296315437 26.528218325002463 27.469893315005983 23.139680063676117 64-65 22.692275891190803 26.337179375380572 27.386969739375576 23.58357499405305 66-67 22.745601884204454 26.50048653431979 27.264042156512986 23.48986942496277 68-69 22.724994183762952 26.3523857874714 27.451998383698335 23.470621645067315 70-71 22.85248910577094 26.352336716537568 27.33239350554384 23.462780672147655 72-73 22.986727422246382 26.236574572689904 27.302095140796762 23.47460286426696 74-75 22.8000623105889 26.734030415780026 27.012582105220133 23.45332516841094 76-77 22.943000728041344 26.45626158868188 27.00374265226252 23.59699503101426 78-79 23.004064890627554 26.508159531705317 27.145948930151214 23.341826647515912 80-81 22.872819260457337 26.703295151464367 27.082452690037723 23.34143289804057 82-83 23.031554447027453 26.58399783818189 26.972767306078982 23.411680408711675 84-85 22.91536963991336 26.485239223520225 26.962055447022742 23.637335689543676 86-87 22.902196308682807 26.435928112589185 27.05391593474895 23.607959643979054 88-89 22.89739049009835 26.341399657348685 27.06350236196627 23.697707490586694 90-91 22.78570998025949 26.487846777474722 27.137588342550593 23.588854899715194 92-93 22.97291391564302 26.230402194163087 27.1273002639099 23.669383626283995 94-95 22.861343512859563 26.138834496990317 27.171159767136743 23.828662223013378 96-97 22.848896150645366 26.109321415479926 27.3325766003753 23.70920583349941 98-99 22.866616864087554 26.16488872966128 26.868359577634948 24.100134828616216 100-101 22.892024155130706 25.621331696122958 27.386166639515842 24.100477509230494 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3336.0 1 1969.0 2 705.5 3 825.0 4 687.0 5 626.5 6 1038.5 7 2242.5 8 3638.0 9 3934.5 10 3291.5 11 2743.5 12 2770.5 13 2992.5 14 3291.5 15 3499.5 16 3580.5 17 3692.0 18 3801.5 19 3956.5 20 4236.0 21 4818.0 22 5557.0 23 6751.5 24 8294.5 25 10374.5 26 12972.5 27 15835.5 28 18428.5 29 21542.0 30 25368.0 31 29296.0 32 33952.5 33 37967.5 34 41582.0 35 45309.0 36 50629.0 37 56686.0 38 60838.0 39 62999.5 40 65684.5 41 72089.0 42 77984.5 43 83071.5 44 88353.5 45 93338.0 46 94935.0 47 97348.0 48 107049.5 49 110040.0 50 104955.0 51 101755.5 52 100333.5 53 100767.5 54 100419.0 55 94371.0 56 84882.5 57 78373.0 58 72825.5 59 63739.0 60 53951.0 61 44832.5 62 36637.0 63 29292.5 64 22680.5 65 17294.0 66 13360.5 67 11317.0 68 9512.0 69 7188.5 70 5386.0 71 4257.5 72 3450.5 73 2891.0 74 2255.5 75 1749.5 76 1276.5 77 781.5 78 487.5 79 336.0 80 225.0 81 146.5 82 117.0 83 84.5 84 61.5 85 45.0 86 32.0 87 26.0 88 17.5 89 18.0 90 17.5 91 12.0 92 9.0 93 7.5 94 7.0 95 7.0 96 4.0 97 2.0 98 1.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27414367603793005 2 0.16891150709642094 3 0.16898651220614405 4 0.18972542504458115 5 0.20315133968501603 6 0.103244533533847 7 0.11925812445972882 8 0.13024637303416295 9 0.13422164384948723 10-11 0.12220207501636049 12-13 0.11220764414575743 14-15 0.12643986371571564 16-17 0.10363831035989327 18-19 0.13071515496993233 20-21 0.15338544938373927 22-23 0.15790450724455604 24-25 0.17914970457362406 26-27 0.16187977805988032 28-29 0.15936710688415648 30-31 0.1490914068520918 32-33 0.1581295225737253 34-35 0.33294768206084036 36-37 0.44631790540730587 38-39 0.41417821589095755 40-41 0.4035649928651389 42-43 0.42749162286680775 44-45 0.431054365578655 46-47 0.4345046006259176 48-49 0.44592412858125957 50-51 0.4391174148738883 52-53 0.4451553262065978 54-55 0.42818542013174654 56-57 0.39111414465110433 58-59 0.3809509522836243 60-61 0.33662293243727226 62-63 0.4425488986437201 64-65 0.44119880666870437 66-67 0.4498806481191531 68-69 0.46043761731267946 70-71 0.45370590871503125 72-73 0.44680543862050603 74-75 0.45154951181049213 76-77 0.4281291662994542 78-79 0.414890764433327 80-81 0.4122468343155878 82-83 0.42424765187128377 84-85 0.4265540589952691 86-87 0.428147917576885 88-89 0.43501088511654856 90-91 0.4517182733073691 92-93 0.45640609266506277 94-95 0.4551872596320624 96-97 0.45348089338586195 98-99 0.4501806685580455 100-101 0.4336232905866712 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2666485.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.56260559597329 #Duplication Level Percentage of deduplicated Percentage of total 1 72.52505428415829 34.49480552743974 2 14.09218303785306 13.405218876313397 3 5.334116263772985 7.611134041707032 4 2.5564468960798297 4.863651017811802 5 1.3926672996417166 3.3119442749634063 6 0.8847789046912852 2.5249434050081305 7 0.5486039370940897 1.8265122881883034 8 0.3945254058931243 1.5011725022468756 9 0.2933796728370396 1.2558511502121414 >10 1.7590482638825715 15.587738045025048 >50 0.14210071852446998 4.689229008554804 >100 0.0724344632798415 6.232406359195253 >500 0.003396892348232703 1.0887981507420605 >1k 0.0010269674541120157 0.8285281255409573 >5k 2.3699248941046517E-4 0.7780672270510247 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9037 0.33891058828382686 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6623 0.24837942084804526 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5048 0.1893128969411041 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3026 0.11348273101105012 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.6253832292324915E-5 0.0 12-13 0.0 0.0 0.0 7.500510972309989E-5 0.0 14-15 0.0 0.0 0.0 7.500510972309989E-5 0.0 16-17 0.0 0.0 0.0 7.500510972309989E-5 0.0 18-19 0.0 0.0 0.0 7.500510972309989E-5 0.0 20-21 0.0 0.0 0.0 7.500510972309989E-5 0.0 22-23 0.0 0.0 0.0 1.5001021944619977E-4 0.0 24-25 0.0 0.0 0.0 3.375229937539495E-4 0.0 26-27 0.0 0.0 0.0 5.062844906309242E-4 0.0 28-29 0.0 0.0 0.0 0.0014625996396004479 0.0 30-31 0.0 0.0 0.0 0.004181534867062819 0.0 32-33 0.0 0.0 0.0 0.010613223025818633 0.0 34-35 0.0 0.0 0.0 0.01991385663148302 0.0 36-37 0.0 0.0 0.0 0.03547741689902625 0.0 38-39 0.0 0.0 0.0 0.05839147791943326 0.0 40-41 0.0 0.0 0.0 0.08535581486488766 0.0 42-43 0.0 0.0 0.0 0.11427028466314268 0.0 44-45 0.0 0.0 0.0 0.1500664732784921 0.0 46-47 0.0 0.0 0.0 0.19542581338353676 0.0 48-49 0.0 0.0 0.0 0.24517295240738277 0.0 50-51 0.0 0.0 0.0 0.3015767949191539 0.0 52-53 0.0 0.0 0.0 0.36105584692957204 0.0 54-55 0.0 0.0 0.0 0.428391684183485 0.0 56-57 0.0 0.0 0.0 0.5100722486719408 0.0 58-59 0.0 0.0 0.0 0.6016534876438457 0.0 60-61 0.0 0.0 0.0 0.7044104879644926 0.0 62-63 0.0 0.0 0.0 0.8122490844688794 0.0 64-65 0.0 0.0 0.0 0.930532142502208 0.0 66-67 0.0 0.0 0.0 1.0714854949493433 0.0 68-69 0.0 0.0 0.0 1.2325777193571312 0.0 70-71 0.0 0.0 0.0 1.4149526436488484 0.0 72-73 0.0 0.0 0.0 1.5994652135676743 0.0 74-75 0.0 0.0 0.0 1.7936721939182108 0.0 76-77 0.0 0.0 0.0 2.0103057020759536 0.0 78-79 0.0 0.0 0.0 2.2586851229239993 0.0 80-81 0.0 0.0 0.0 2.5382291668619925 0.0 82-83 0.0 0.0 0.0 2.827298859734819 0.0 84-85 0.0 0.0 0.0 3.1209438643007554 0.0 86-87 0.0 0.0 0.0 3.437952960545437 0.0 88-89 0.0 0.0 0.0 3.786839228422436 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 11515 0.0 29.50725 1 GTACATG 9525 0.0 26.20598 1 GGTATCA 8200 0.0 25.984365 1 TACATGG 9650 0.0 24.960705 2 ATCAACG 13790 0.0 24.17194 3 TCAACGC 13820 0.0 24.153324 4 CAACGCA 13915 0.0 24.0225 5 ACATGGG 9740 0.0 23.659075 3 TATCAAC 14290 0.0 23.458899 2 AACGCAG 14200 0.0 23.42786 6 GAGTACT 7655 0.0 21.232252 12-13 ACGCAGA 15860 0.0 20.796482 7 CGCAGAG 16150 0.0 20.540422 8 CATGGGG 6120 0.0 20.06599 4 AGTACTT 8090 0.0 19.856298 12-13 GTACTTT 8170 0.0 19.81003 14-15 AGAGTAC 12770 0.0 19.777493 10-11 GCAGAGT 17255 0.0 19.031704 9 TACACTG 2135 0.0 17.988686 5 GTGTAAG 920 0.0 17.537624 1 >>END_MODULE