FastQCFastQC Report
Fri 27 May 2016
SRR939243_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939243_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2666485
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92320.3462235864818291No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75790.284231863295687No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54380.20393889333710857No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33410.12529603579243834No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83800.030.7262251
GTATCAA120750.030.3106481
ATCAACG146550.024.6433013
TCAACGC147700.024.547824
CAACGCA148500.024.383625
TATCAAC150000.024.2346942
AACGCAG153500.023.5893616
GTACATG98650.023.1097051
TACATGG98750.022.5390592
ACATGGG97800.021.9816043
GAGTACT85650.021.3328912-13
ACGCAGA170300.021.2901527
CGCAGAG173300.020.8942138
AGAGTAC139550.020.23501210-11
AGTACTT89400.020.09299712-13
GTACTTT91050.020.01555314-15
GCAGAGT183850.019.437469
CATGGGG61800.019.2745954
GTGTAGC13600.018.5478231
GGACCGA4900.017.4331746
Produced by FastQC (version 0.11.5)