##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939243_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2666485 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16624132519028 33.0 31.0 34.0 30.0 34.0 2 32.39999099938683 34.0 31.0 34.0 31.0 34.0 3 32.48464514145026 34.0 31.0 34.0 30.0 34.0 4 36.050132290262276 37.0 35.0 37.0 35.0 37.0 5 35.98756265270571 37.0 35.0 37.0 35.0 37.0 6 36.01508540269306 37.0 35.0 37.0 35.0 37.0 7 35.9537878517974 37.0 35.0 37.0 35.0 37.0 8 35.91851407377128 37.0 35.0 37.0 35.0 37.0 9 37.60140484570511 39.0 37.0 39.0 35.0 39.0 10-11 37.50536980331785 39.0 37.0 39.0 35.0 39.0 12-13 37.417042098492956 39.0 37.0 39.0 34.0 39.0 14-15 38.65901289525349 40.0 38.0 41.0 34.0 41.0 16-17 38.65132505901965 40.0 38.0 41.0 34.0 41.0 18-19 38.75949424054514 40.0 38.0 41.0 34.0 41.0 20-21 38.73153102305095 40.0 38.0 41.0 34.0 41.0 22-23 38.63951250428936 40.0 38.0 41.0 34.0 41.0 24-25 38.56133017811838 40.0 38.0 41.0 34.0 41.0 26-27 38.37733439340555 40.0 38.0 41.0 34.0 41.0 28-29 38.18751146171833 40.0 38.0 41.0 33.0 41.0 30-31 37.97656409092869 40.0 37.0 41.0 33.0 41.0 32-33 37.92166447589242 40.0 37.0 41.0 33.0 41.0 34-35 37.8122299581659 40.0 37.0 41.0 33.0 41.0 36-37 37.6417155918747 40.0 37.0 41.0 33.0 41.0 38-39 37.48909275694407 40.0 37.0 41.0 32.0 41.0 40-41 37.277063999985 40.0 36.0 41.0 31.5 41.0 42-43 37.06651396876412 40.0 36.0 41.0 31.0 41.0 44-45 36.80070617310804 39.0 36.0 41.0 31.0 41.0 46-47 36.476844235013516 39.0 35.0 41.0 30.0 41.0 48-49 36.139196920290196 39.0 35.0 40.0 29.5 41.0 50-51 35.85531926862518 39.0 35.0 40.0 28.5 41.0 52-53 35.55413568799375 38.0 35.0 40.0 28.0 41.0 54-55 35.26891356973694 38.0 34.0 40.0 27.5 41.0 56-57 34.89880141834662 38.0 34.0 40.0 26.5 41.0 58-59 34.525932266635664 37.5 34.0 40.0 26.0 41.0 60-61 34.13604258040079 37.0 33.0 40.0 26.0 41.0 62-63 33.72646555296579 36.5 33.0 39.0 25.0 40.5 64-65 33.35410531092431 36.0 32.5 39.0 23.5 40.0 66-67 32.87662109481208 35.5 32.0 39.0 22.5 40.0 68-69 32.35145800557663 35.0 31.0 38.0 21.5 40.0 70-71 31.81913942887359 35.0 31.0 37.5 20.0 39.5 72-73 31.275367009377515 34.5 30.5 37.0 18.5 39.0 74-75 30.706283928092603 34.0 30.0 36.0 16.0 39.0 76-77 28.842302694371057 32.5 27.0 34.5 10.5 36.5 78-79 29.814021642724413 34.0 29.0 35.0 11.0 37.0 80-81 29.80263436696625 34.0 29.0 35.0 7.5 37.0 82-83 29.39895574135988 34.0 29.0 35.0 6.0 36.5 84-85 28.872861838712762 34.0 29.0 35.0 2.0 36.0 86-87 28.175909483833586 33.0 27.5 35.0 2.0 35.5 88-89 27.493200599290827 33.0 26.0 35.0 2.0 35.0 90-91 26.891312720679096 32.0 25.0 34.0 2.0 35.0 92-93 26.40215864705783 32.0 25.0 34.0 2.0 35.0 94-95 25.717017159293977 32.0 23.5 34.0 2.0 35.0 96-97 24.767920127058655 31.0 19.0 34.0 2.0 35.0 98-99 23.50278231454518 31.0 2.0 34.0 2.0 35.0 100-101 19.150124602238527 25.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2758087456924869 1101 2 0.3028035089821728 1101 3 0.2931739177007273 1101 4 0.22908361451996484 1101 5 0.2795544869826827 1101 6 0.25846108848760707 1101 7 0.29687205984360077 1101 8 0.2950347977444494 1101 9 0.35377519181351147 1101 10-11 0.4085921379474229 1101 12-13 0.3810246446272956 1101 14-15 0.4806942018817182 1101 16-17 0.3677688826807355 1101 18-19 0.3622277255548312 1101 20-21 0.3995141939618705 1101 22-23 0.4011366153486051 1101 24-25 0.47639229153283935 1101 26-27 0.5375979193275384 1101 28-29 0.55399005137199 1101 30-31 0.5335937884081119 1101 32-33 0.6595736178016196 1101 34-35 0.730480443903005 1101 36-37 0.7382330821211625 1101 38-39 0.7185299500859372 1101 40-41 0.7726588265700585 1101 42-43 0.8418233823423122 1101 44-45 0.879084676948807 1101 46-47 0.9367596554613016 1101 48-49 0.9583545171494876 1101 50-51 0.9958995236744244 1101 52-53 1.0431973444477407 1101 54-55 0.964032968611491 1101 56-57 0.9980101073422034 1101 58-59 0.9786482212268979 1101 60-61 0.9280062700636194 1101 62-63 0.9455748202354641 1101 64-65 0.9280512673687937 1101 66-67 0.9667600440946131 1101 68-69 0.8989223881263158 1101 70-71 0.9264208856836724 1101 72-73 1.0640817305480397 1101 74-75 0.9868332360473921 1101 76-77 1.153979348010342 1101 78-79 1.1928372769699997 1101 80-81 1.2707942144298592 1101 82-83 1.136925007838208 1101 84-85 1.0146630845225424 1101 86-87 1.0305644191417684 1101 88-89 1.1567431361369032 1101 90-91 1.3567002949069078 1101 92-93 1.4570289725613925 1101 94-95 1.2553636321989146 1101 96-97 0.915353840001611 1101 98-99 0.8644911487132063 1101 100-101 0.8095440193391212 1102 1 0.19446289449680876 1102 2 0.206214979839622 1102 3 0.22841095898967723 1102 4 0.12638784408191128 1102 5 0.2101361142358371 1102 6 0.2135621777243486 1102 7 0.22006656246455947 1102 8 0.23738790419799471 1102 9 0.16339965594316652 1102 10-11 0.23157522280239107 1102 12-13 0.25100099479647753 1102 14-15 0.28529652446058407 1102 16-17 0.29373215649302864 1102 18-19 0.2505656612969318 1102 20-21 0.28074619156240743 1102 22-23 0.39485699919294603 1102 24-25 0.30025082520884894 1102 26-27 0.2749955933011705 1102 28-29 0.31325994041429794 1102 30-31 0.2913394527494404 1102 32-33 0.3209893152906389 1102 34-35 0.3553190579401786 1102 36-37 0.45817176838340146 1102 38-39 0.4424942357386641 1102 40-41 0.4061196311124817 1102 42-43 0.3964379866299623 1102 44-45 0.44550243431740455 1102 46-47 0.46420798178759526 1102 48-49 0.4525482854535383 1102 50-51 0.33477212313902527 1102 52-53 0.30101066592168735 1102 54-55 0.23354989668463588 1102 56-57 0.20480003927137602 1102 58-59 0.19552294434977568 1102 60-61 0.30056587298089 1102 62-63 0.23653173341541844 1102 64-65 0.11340250700260412 1102 66-67 0.21902461881997226 1102 68-69 0.26986640766673275 1102 70-71 0.1430622563396149 1102 72-73 0.12191220332056218 1102 74-75 0.16351789864945943 1102 76-77 0.21693624912405696 1102 78-79 0.14489207149054906 1102 80-81 0.38042893486369067 1102 82-83 0.39171315051632405 1102 84-85 0.22106474207919646 1102 86-87 0.13688889585478847 1102 88-89 0.4027242162722544 1102 90-91 0.44251625716371024 1102 92-93 0.3619293312734797 1102 94-95 0.1959283494782902 1102 96-97 -0.2820485570431934 1102 98-99 -0.6449957171843224 1102 100-101 -0.4179942435704618 1103 1 0.18745759271862283 1103 2 0.22367801162889833 1103 3 0.1950173006586695 1103 4 0.11828833876423062 1103 5 0.13119578193094839 1103 6 0.14993260153708832 1103 7 0.18246099371863522 1103 8 0.19457001863107592 1103 9 0.2609230936644735 1103 10-11 0.190864159596714 1103 12-13 0.26938646141481826 1103 14-15 0.3209960169443846 1103 16-17 0.21461991095207367 1103 18-19 0.24531649044046588 1103 20-21 0.2547494272021993 1103 22-23 0.36031139933353984 1103 24-25 0.2774402006095542 1103 26-27 0.2925592149284526 1103 28-29 0.2687516830869896 1103 30-31 0.44216061847004084 1103 32-33 0.5657585714519158 1103 34-35 0.44391550885320896 1103 36-37 0.43037956213029815 1103 38-39 0.42913298583476944 1103 40-41 0.4323503102037307 1103 42-43 0.38268268759776447 1103 44-45 0.470429564495106 1103 46-47 0.45133761318365373 1103 48-49 0.4149758130767296 1103 50-51 0.37505112697589027 1103 52-53 0.3067217406894187 1103 54-55 0.42219847799214705 1103 56-57 0.41781094459725665 1103 58-59 0.3403484643385397 1103 60-61 0.40755913577415015 1103 62-63 0.3874745174530787 1103 64-65 0.37405274515193554 1103 66-67 0.16963356310650113 1103 68-69 0.12689782522613058 1103 70-71 0.2823399432589504 1103 72-73 0.42202516455243 1103 74-75 0.2821168585274094 1103 76-77 0.4464962061594768 1103 78-79 0.41403449109238366 1103 80-81 0.5627478717100374 1103 82-83 0.7422823213496663 1103 84-85 0.6479558021284397 1103 86-87 0.42076064496754384 1103 88-89 0.5629787866062301 1103 90-91 0.3843889162098293 1103 92-93 0.30103107269168916 1103 94-95 0.32158008780364966 1103 96-97 0.08643608306197237 1103 98-99 0.06288120421165644 1103 100-101 -0.019541852914219504 1104 1 0.12669663898471129 1104 2 0.1135036497191706 1104 3 0.11928573421730704 1104 4 0.07894064087459896 1104 5 0.1581183617347861 1104 6 0.09781646258764454 1104 7 0.06599141559621557 1104 8 0.1455143587643164 1104 9 0.12350417847274997 1104 10-11 0.1459541967679172 1104 12-13 0.17116623040173096 1104 14-15 0.149364678884929 1104 16-17 0.07150976832590317 1104 18-19 0.14106805616633267 1104 20-21 0.23924349031130987 1104 22-23 0.31997426771332016 1104 24-25 0.2616747519412428 1104 26-27 0.32384199650233114 1104 28-29 0.1738352865587629 1104 30-31 0.18081047635163827 1104 32-33 0.17733377764764668 1104 34-35 0.182962496208539 1104 36-37 0.14238597044113277 1104 38-39 0.15679593153434723 1104 40-41 0.26331048358471776 1104 42-43 0.20093139999236342 1104 44-45 0.27621132533228376 1104 46-47 0.24874113942794907 1104 48-49 0.3402058394693128 1104 50-51 0.23071151304402093 1104 52-53 0.32380047186002514 1104 54-55 0.31694517487893137 1104 56-57 0.3060707125686335 1104 58-59 0.23496446768982793 1104 60-61 0.31663206565202273 1104 62-63 0.4023313142670517 1104 64-65 0.25320075024350075 1104 66-67 0.09841164490623555 1104 68-69 0.24339610976529258 1104 70-71 0.13871423915834313 1104 72-73 0.22509683769511213 1104 74-75 0.06383254440592978 1104 76-77 0.2188247495122262 1104 78-79 0.4269192003526463 1104 80-81 0.5362153529657583 1104 82-83 0.3873247376048248 1104 84-85 0.42938673189321364 1104 86-87 0.36138567742454697 1104 88-89 0.6152191276903913 1104 90-91 0.7007001618548188 1104 92-93 0.7046535339769768 1104 94-95 0.5892254461347441 1104 96-97 0.4995772459286236 1104 98-99 0.39668158180922575 1104 100-101 0.2386811650735936 1105 1 0.06641210172375622 1105 2 0.06731486332993342 1105 3 0.08382613065004563 1105 4 0.01398402287883016 1105 5 0.02181498470581289 1105 6 0.07584823183918843 1105 7 0.04814347262706775 1105 8 0.010991863701008242 1105 9 0.08580094408259953 1105 10-11 0.06971800526392968 1105 12-13 0.15193172358403473 1105 14-15 0.17227095765773726 1105 16-17 0.10567318429870198 1105 18-19 0.1363823734196572 1105 20-21 0.14058017544212476 1105 22-23 0.1215727985838555 1105 24-25 0.10140645295835782 1105 26-27 0.19384616685112377 1105 28-29 0.25529528163960435 1105 30-31 0.25404370822612066 1105 32-33 0.12222130022768596 1105 34-35 0.14854540972581987 1105 36-37 -0.04723420810139345 1105 38-39 0.19796536520546937 1105 40-41 0.21176993654825793 1105 42-43 0.11394968922946447 1105 44-45 0.048081747799557206 1105 46-47 0.13638796052559599 1105 48-49 0.22906779699794555 1105 50-51 0.32306980462011836 1105 52-53 0.34108491292531085 1105 54-55 0.204229509371757 1105 56-57 0.18618075705141734 1105 58-59 0.11548993444216649 1105 60-61 0.1487465699119781 1105 62-63 0.29412156058685923 1105 64-65 0.2887603356093038 1105 66-67 0.2107953932785449 1105 68-69 0.15664850785606887 1105 70-71 0.1710483948758572 1105 72-73 0.2202282990363713 1105 74-75 0.27490849439427834 1105 76-77 0.2803124338019565 1105 78-79 0.2347692579438423 1105 80-81 0.2807171252938794 1105 82-83 0.30294647995803814 1105 84-85 0.37898728147058236 1105 86-87 0.4186863960314575 1105 88-89 0.41203101242722084 1105 90-91 0.4397148545517169 1105 92-93 0.4273495985643798 1105 94-95 0.7174615680861756 1105 96-97 0.4258059633374316 1105 98-99 0.4032542983048728 1105 100-101 0.2113342501080453 1106 1 0.012730959903812789 1106 2 0.04752633202151202 1106 3 0.005838141044471001 1106 4 0.02109074421635171 1106 5 -0.059731942561498386 1106 6 0.06235939017790315 1106 7 0.09545813690841243 1106 8 -0.03837363772779412 1106 9 0.04660243686678456 1106 10-11 0.05502378498739802 1106 12-13 0.0763527909062347 1106 14-15 -0.07120137610434085 1106 16-17 -0.03471850015993283 1106 18-19 -0.01071197243443578 1106 20-21 0.06909482361820807 1106 22-23 0.05415722337552609 1106 24-25 0.10919540376548298 1106 26-27 0.20738132475892002 1106 28-29 0.1931668317933699 1106 30-31 0.22595790360477253 1106 32-33 0.13021619170212517 1106 34-35 0.26823679253714516 1106 36-37 0.20822226741315575 1106 38-39 0.28458728806559463 1106 40-41 0.2300709069994511 1106 42-43 0.18360320289826149 1106 44-45 0.355920673904663 1106 46-47 0.11154987015328288 1106 48-49 0.23108292778795914 1106 50-51 0.14435642506030888 1106 52-53 0.3998498919122895 1106 54-55 0.23247555052289215 1106 56-57 0.19779681726770804 1106 58-59 0.2382838256489066 1106 60-61 0.2840930480817221 1106 62-63 0.2645135769445872 1106 64-65 0.17788604266319652 1106 66-67 0.2767516326550563 1106 68-69 0.2974293576048055 1106 70-71 0.28754701722256826 1106 72-73 0.3525865971666349 1106 74-75 0.433097655658063 1106 76-77 0.41997360019331254 1106 78-79 0.5237297907887957 1106 80-81 0.49861404940542187 1106 82-83 0.634529249607013 1106 84-85 0.572426642962018 1106 86-87 0.5749998513295473 1106 88-89 0.7877863549144415 1106 90-91 1.0209261420590394 1106 92-93 0.8948118572846191 1106 94-95 1.0743649740237977 1106 96-97 0.9918428650757107 1106 98-99 0.7790324248487508 1106 100-101 0.4631854717143469 1107 1 0.038601014787857935 1107 2 0.08654185606368259 1107 3 0.05664038399361715 1107 4 0.07667458779153691 1107 5 0.060957571307568514 1107 6 0.09870222511334248 1107 7 0.050360159898374945 1107 8 0.06476170590555341 1107 9 0.06246791808036534 1107 10-11 0.06241561635907544 1107 12-13 0.03872252061023573 1107 14-15 0.0899965818707642 1107 16-17 0.11783535282696533 1107 18-19 0.09433631547944543 1107 20-21 0.1361033258865305 1107 22-23 0.1818228404773521 1107 24-25 0.14920675862943256 1107 26-27 0.15818178318346554 1107 28-29 0.1554782670270214 1107 30-31 0.10158228893519095 1107 32-33 -0.01816934228050826 1107 34-35 0.11384487920952324 1107 36-37 0.08084237252826654 1107 38-39 0.16363544907994054 1107 40-41 0.1117040510409808 1107 42-43 0.1090644033677961 1107 44-45 0.03514887362337049 1107 46-47 -0.009751074719446251 1107 48-49 -0.01715839016938503 1107 50-51 0.012420176210341083 1107 52-53 0.09964797625817567 1107 54-55 0.12887748626943107 1107 56-57 0.1477001885651461 1107 58-59 0.17936563090042767 1107 60-61 0.15454072582036815 1107 62-63 0.055788309133710356 1107 64-65 0.041998988529975634 1107 66-67 0.05624757715766293 1107 68-69 0.06572522914019174 1107 70-71 0.17075860632190043 1107 72-73 0.13698159045153702 1107 74-75 0.22702372875219012 1107 76-77 0.2292980056161511 1107 78-79 0.27382516371477905 1107 80-81 0.26462639038445346 1107 82-83 0.5026690743799165 1107 84-85 0.41248181781898907 1107 86-87 0.48422901601340484 1107 88-89 0.6908640650124696 1107 90-91 0.8219759051799187 1107 92-93 0.733462346905295 1107 94-95 0.9213183939660574 1107 96-97 0.7626130130194078 1107 98-99 0.622341713365401 1107 100-101 0.3979347261796278 1108 1 0.018067296411388156 1108 2 0.026604693327449525 1108 3 0.039591776215821994 1108 4 0.0645615329614202 1108 5 0.06447964500257797 1108 6 0.05265966008344236 1108 7 0.034089475094972954 1108 8 0.0174610894838807 1108 9 0.08857757831376034 1108 10-11 0.03991149066364841 1108 12-13 0.08138916637904003 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-0.6946189900791282 2316 50-51 -0.6837023066998711 2316 52-53 -0.825270033166646 2316 54-55 -0.7920717235461936 2316 56-57 -0.7383836565472919 2316 58-59 -0.5699814294750567 2316 60-61 -0.5353613220021671 2316 62-63 -0.6320951239877175 2316 64-65 -0.34281884121849515 2316 66-67 -0.2379826871700672 2316 68-69 -0.2730064830618204 2316 70-71 -0.19109884530382004 2316 72-73 0.021922093121645503 2316 74-75 -0.07587920942797766 2316 76-77 -0.22427571785687306 2316 78-79 -0.28446540099791306 2316 80-81 -0.2500430699638194 2316 82-83 -0.2740071506321371 2316 84-85 -0.15117933017102914 2316 86-87 -0.21903378841411936 2316 88-89 -0.4196022910882107 2316 90-91 -0.5504923369126189 2316 92-93 -0.26741921627254683 2316 94-95 0.010068433284299516 2316 96-97 0.18859823192734382 2316 98-99 0.39655491518001185 2316 100-101 1.0338110672527172 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 11.0 4 29.0 5 70.0 6 153.0 7 437.0 8 910.0 9 1703.0 10 2871.0 11 4174.0 12 5997.0 13 8292.0 14 10388.0 15 13773.0 16 17674.0 17 23361.0 18 23197.0 19 18254.0 20 18246.0 21 19436.0 22 21195.0 23 23599.0 24 27115.0 25 31670.0 26 37892.0 27 45144.0 28 54615.0 29 65793.0 30 79254.0 31 95958.0 32 118803.0 33 151919.0 34 203090.0 35 285437.0 36 419012.0 37 541850.0 38 284254.0 39 10907.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.20159819497979 19.574842530788754 15.268101908432829 22.955457365798626 2 16.216367240018226 20.519260374613022 39.12251522134945 24.1418571640193 3 19.670802573425313 23.92531741224871 29.57218960541687 26.831690408909108 4 13.75713720497209 16.930678402466167 35.86946860754889 33.44271578501286 5 14.444446527919713 36.874499575283565 33.55803614121212 15.1230177555846 6 32.98105933466717 35.60361299613536 16.73063977483466 14.684687894362803 7 29.414116336675434 32.11812554730291 20.035664929673334 18.432093186348318 8 26.354245382966713 35.4985308374133 19.036784380935952 19.110439398684033 9 26.2639392308601 17.094189541662523 19.81676251694647 36.825108710530905 10-11 25.626058275219997 26.312336277908933 26.56409092869452 21.49751451817655 12-13 26.18261494064283 23.432046308154746 27.38895962287431 22.99637912832812 14-15 23.00345477292706 25.035252784970012 25.565375914697263 26.395916527405667 16-17 22.772100666877183 28.27177476637547 26.126644409925078 22.82948015682227 18-19 22.683897596985396 26.691122091863296 28.526366556109096 22.09861375504222 20-21 23.363927298283564 25.880255444950084 28.440611599279876 22.315205657486477 22-23 23.05523938818332 25.633183760643696 28.510267261957225 22.801309589215766 24-25 23.005979782372673 25.9123715303105 28.46190771746325 22.61974096985357 26-27 22.707672506321064 26.185644605888957 28.50893049343667 22.59775239435331 28-29 22.596433132007117 26.375771849457248 28.689885748466615 22.337909270069023 30-31 22.782970839888467 25.97515080714874 28.376345638546624 22.86553271441617 32-33 22.677701168392097 26.036373727960218 28.364326069713496 22.921599033934186 34-35 23.192435476872408 26.179425280157453 28.08992640768783 22.538212835282305 36-37 23.209301880752705 25.83839512402864 28.290839044900995 22.66146395031766 38-39 22.783799905228612 26.115349946266836 28.38568168176277 22.715168466741787 40-41 22.96371692484479 26.036895696964663 28.27548742062591 22.72389995756464 42-43 22.944824481249917 26.0297886934524 27.940715133407075 23.084671691890605 44-45 22.959844377414022 26.194424724099562 27.87720456891951 22.968526329566906 46-47 23.087156956777328 26.54330189678725 27.556096842938217 22.81344430349721 48-49 23.23380421136914 26.273006753304223 27.587402457741454 22.90578657758518 50-51 23.087602658633113 26.618058318766707 27.392786678182464 22.901552344417723 52-53 23.34740618023932 26.59289907209217 27.08232662318241 22.977368124486098 54-55 23.05045781243847 26.41559956272021 27.273301743681287 23.26064088116003 56-57 23.09373306506204 26.67567988209064 27.308298846220918 22.9222882066264 58-59 23.177189837105892 26.34050716961715 27.342110252095924 23.140192741181032 60-61 23.23832236249955 26.452616648865312 27.514153575671752 22.794907412963386 62-63 22.89570831924625 26.593415701291175 27.438265753767322 23.072610225695257 64-65 22.79484894715288 26.338710451665936 27.40518975549825 23.46125084568293 66-67 22.843971893192933 26.500339493596687 27.33674445798502 23.31894415522536 68-69 22.79083887817549 26.418125727092505 27.385407220558335 23.405628174173675 70-71 22.929793342171436 26.435644678293706 27.27191414915141 23.362647830383445 72-73 23.051770401858626 26.36084583262235 27.22522346834878 23.362160297170245 74-75 22.910165050650022 26.80698125190677 26.827120127643724 23.455733569799484 76-77 22.97407597757886 26.496224731757255 27.043538055899464 23.486161234764424 78-79 23.009641924934744 26.59346779274549 27.105341123878674 23.29154915844109 80-81 22.923644106904213 26.777338142919106 27.006235372806962 23.29278237736972 82-83 23.05977719732157 26.676786106053473 26.915827390740994 23.347609305883964 84-85 22.885334063383066 26.57528544132069 26.903376542526956 23.636003952769283 86-87 22.88492431014165 26.49817040138544 26.973993683220215 23.642911605252696 88-89 22.86948119870865 26.49126247431551 26.91162427604461 23.72763205093123 90-91 22.722554334797678 26.595382846570885 27.030493668970195 23.65156914966124 92-93 22.86695781150091 26.382391050390307 27.012884002722686 23.737767135386097 94-95 22.752443760231166 26.299997937359482 27.055224387161374 23.892333915247978 96-97 22.69907762466318 26.238287483334805 27.187833421151815 23.8748014708502 98-99 22.608098871828336 26.381590728609694 26.762560789354517 24.247749610207457 100-101 22.73709246531611 25.699992368202896 27.435316769270894 24.1275983972101 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 890.0 1 613.5 2 462.0 3 599.0 4 513.0 5 494.5 6 859.0 7 2043.5 8 3352.5 9 3660.5 10 2963.0 11 2285.5 12 2263.5 13 2433.5 14 2752.5 15 3003.5 16 3152.0 17 3379.0 18 3561.0 19 3757.0 20 4192.0 21 4798.0 22 5552.0 23 6933.5 24 8476.0 25 10559.5 26 13458.0 27 16326.0 28 18907.5 29 22172.5 30 26244.0 31 30164.0 32 34609.5 33 38679.0 34 42202.5 35 46186.0 36 51663.5 37 57460.5 38 61073.0 39 63473.0 40 66632.5 41 72941.5 42 79461.5 43 84383.5 44 89497.0 45 94321.5 46 95710.0 47 98985.0 48 108484.5 49 111036.5 50 105657.5 51 101854.0 52 100165.5 53 100529.5 54 100197.0 55 93954.0 56 84525.5 57 77733.0 58 71719.5 59 62811.5 60 53067.5 61 43515.5 62 35560.5 63 28706.0 64 22008.0 65 16704.5 66 12848.0 67 10746.5 68 8851.5 69 6603.0 70 4943.5 71 3825.0 72 3084.5 73 2646.0 74 2151.5 75 1785.0 76 1304.0 77 702.0 78 392.0 79 269.0 80 175.5 81 110.5 82 67.5 83 36.5 84 19.5 85 9.0 86 9.0 87 8.0 88 7.0 89 3.5 90 0.0 91 1.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2713309844233138 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0010875740909849483 16-17 0.0010125689812618484 18-19 3.750255486154994E-5 20-21 1.875127743077497E-5 22-23 0.0 24-25 0.0 26-27 1.1250766458464984E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0014250970847388977 36-37 0.006956723926817515 38-39 0.002568925008016171 40-41 0.0028689454469085704 42-43 1.312589420154248E-4 44-45 0.001275086865292698 46-47 9.375638715387486E-5 48-49 4.687819357693743E-4 50-51 7.500510972309989E-5 52-53 9.375638715387486E-5 54-55 0.0 56-57 0.0011250766458464983 58-59 0.0022314020142622216 60-61 7.875536520925488E-4 62-63 0.001331340697585023 64-65 3.750255486154994E-5 66-67 5.062844906309242E-4 68-69 0.0016126098590466477 70-71 0.0 72-73 0.0 74-75 1.875127743077497E-5 76-77 0.25029205114598435 78-79 1.8751277430774973E-4 80-81 1.6876149687697472E-4 82-83 0.0 84-85 0.0 86-87 0.10448211784427815 88-89 0.36474984858343473 90-91 0.26861204919585147 92-93 0.0 94-95 0.0 96-97 0.0 98-99 9.375638715387486E-5 100-101 3.5627427118472443E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2666485.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.35909106569625 #Duplication Level Percentage of deduplicated Percentage of total 1 71.51911914600345 33.155613574279535 2 14.56963798420951 13.508703482083915 3 5.451295494706914 7.581513127954125 4 2.643802554802982 4.902571335913273 5 1.4220918589955218 3.296344299747933 6 0.9347073339326828 2.599930944813563 7 0.5960810809703975 1.9343643980671714 8 0.44766632098309095 1.6602722993200254 9 0.338619077188737 1.4128265372377218 >10 1.8430879360467716 15.8484978128756 >50 0.1507848827229981 4.797526137993649 >100 0.07816030109383586 6.4987057045718775 >500 0.0037297918653146425 1.1890553177153984 >1k 9.729891822528518E-4 0.777755621622658 >5k 2.4324729556321294E-4 0.8363194058036112 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9232 0.3462235864818291 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7579 0.284231863295687 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5438 0.20393889333710857 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3341 0.12529603579243834 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.875127743077497E-5 0.0 12-13 0.0 0.0 0.0 3.750255486154994E-5 0.0 14-15 0.0 0.0 0.0 3.750255486154994E-5 0.0 16-17 0.0 0.0 0.0 3.750255486154994E-5 0.0 18-19 0.0 0.0 0.0 3.750255486154994E-5 0.0 20-21 0.0 0.0 0.0 3.750255486154994E-5 0.0 22-23 0.0 0.0 0.0 1.1250766458464983E-4 0.0 24-25 0.0 0.0 0.0 3.0002043889239955E-4 0.0 26-27 0.0 0.0 0.0 4.6878193576937424E-4 0.0 28-29 0.0 0.0 0.0 0.001406345807308123 0.0 30-31 0.0 0.0 0.0 0.004106529757339719 0.0 32-33 0.0 0.0 0.0 0.010481964083803209 0.0 34-35 0.0 0.0 0.0 0.01976384641203682 0.0 36-37 0.0 0.0 0.0 0.03525240156985694 0.0 38-39 0.0 0.0 0.0 0.058166462590263965 0.0 40-41 0.0 0.0 0.0 0.08490578420654907 0.0 42-43 0.0 0.0 0.0 0.11361398995306554 0.0 44-45 0.0 0.0 0.0 0.1490914068520918 0.0 46-47 0.0 0.0 0.0 0.19407572140852097 0.0 48-49 0.0 0.0 0.0 0.2440103732066747 0.0 50-51 0.0 0.0 0.0 0.30018920038927654 0.0 52-53 0.0 0.0 0.0 0.3594619883479562 0.0 54-55 0.0 0.0 0.0 0.426310292388669 0.0 56-57 0.0 0.0 0.0 0.5075783287736477 0.0 58-59 0.0 0.0 0.0 0.5982595064288754 0.0 60-61 0.0 0.0 0.0 0.7005102222588915 0.0 62-63 0.0 0.0 0.0 0.8078425342726474 0.0 64-65 0.0 0.0 0.0 0.9253942924861756 0.0 66-67 0.0 0.0 0.0 1.0649037965711414 0.0 68-69 0.0 0.0 0.0 1.224758436668498 0.0 70-71 0.0 0.0 0.0 1.4055957562108918 0.0 72-73 0.0 0.0 0.0 1.5887207315998402 0.0 74-75 0.0 0.0 0.0 1.780996330375007 0.0 76-77 0.0 0.0 0.0 1.995548446737934 0.0 78-79 0.0 0.0 0.0 2.2418839783460247 0.0 80-81 0.0 0.0 0.0 2.5182215538433557 0.0 82-83 0.0 0.0 0.0 2.8044973063789973 0.0 84-85 0.0 0.0 0.0 3.094467060568501 0.0 86-87 0.0 0.0 0.0 3.407707150049597 0.0 88-89 0.0 0.0 0.0 3.7503117399872865 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8380 0.0 30.726225 1 GTATCAA 12075 0.0 30.310648 1 ATCAACG 14655 0.0 24.643301 3 TCAACGC 14770 0.0 24.54782 4 CAACGCA 14850 0.0 24.38362 5 TATCAAC 15000 0.0 24.234694 2 AACGCAG 15350 0.0 23.589361 6 GTACATG 9865 0.0 23.109705 1 TACATGG 9875 0.0 22.539059 2 ACATGGG 9780 0.0 21.981604 3 GAGTACT 8565 0.0 21.33289 12-13 ACGCAGA 17030 0.0 21.290152 7 CGCAGAG 17330 0.0 20.894213 8 AGAGTAC 13955 0.0 20.235012 10-11 AGTACTT 8940 0.0 20.092997 12-13 GTACTTT 9105 0.0 20.015553 14-15 GCAGAGT 18385 0.0 19.43746 9 CATGGGG 6180 0.0 19.274595 4 GTGTAGC 1360 0.0 18.547823 1 GGACCGA 490 0.0 17.433174 6 >>END_MODULE