FastQCFastQC Report
Fri 27 May 2016
SRR939242_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939242_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2720747
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92330.3393553314586031No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67660.24868170395850844No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51240.18833063125678354No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29940.11004330795917446No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA110850.032.7759861
GGTATCA74650.029.4565011
GTACATG97400.026.672171
ATCAACG135900.026.5555523
TCAACGC137150.026.3135244
CAACGCA138050.026.0732735
TATCAAC140600.025.7353082
AACGCAG141400.025.5057336
TACATGG99300.025.263682
ACATGGG98100.024.4608553
GTCCCGA3750.022.748036
ACGCAGA158500.022.3653077
CGCAGAG159950.022.1329298
GAGTACT81650.021.01178212-13
CATGGGG60150.020.6564564
AGTACTT85200.020.47004112-13
GCAGAGT171600.020.4089989
AGAGTAC133650.020.28287910-11
GTACTTT85950.019.87805614-15
TAGCGCG2001.30387125E-518.969254
Produced by FastQC (version 0.11.5)