##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939242_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2720747 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.03171151158119 33.0 31.0 34.0 30.0 34.0 2 32.138305766761846 34.0 31.0 34.0 30.0 34.0 3 32.193527733376165 34.0 31.0 34.0 30.0 34.0 4 35.689348182686594 37.0 35.0 37.0 35.0 37.0 5 35.7252331804464 37.0 35.0 37.0 35.0 37.0 6 35.81071668920337 37.0 36.0 37.0 35.0 37.0 7 35.733686006085826 37.0 35.0 37.0 35.0 37.0 8 35.71800281319799 37.0 35.0 37.0 35.0 37.0 9 37.383762988620404 39.0 37.0 39.0 35.0 39.0 10-11 37.29123362076665 39.0 37.0 39.0 34.0 39.0 12-13 37.19709826014694 39.0 37.0 39.0 34.0 39.0 14-15 38.47344130123088 40.0 38.0 41.0 34.0 41.0 16-17 38.40681731892013 40.0 38.0 41.0 34.0 41.0 18-19 38.33050371828031 40.0 38.0 41.0 34.0 41.0 20-21 38.22388741033253 40.0 38.0 41.0 34.0 41.0 22-23 38.14489347962159 40.0 38.0 41.0 33.5 41.0 24-25 38.174403389951365 40.0 38.0 41.0 34.0 41.0 26-27 38.09402858847221 40.0 38.0 41.0 33.0 41.0 28-29 37.95473467397005 40.0 38.0 41.0 33.0 41.0 30-31 37.78662440866424 40.0 38.0 41.0 33.0 41.0 32-33 37.59351806691324 40.0 37.0 41.0 32.5 41.0 34-35 37.291862124629745 40.0 37.0 41.0 31.5 41.0 36-37 37.051294185016104 40.0 37.0 41.0 31.0 41.0 38-39 36.7286103779587 39.5 36.0 41.0 30.5 41.0 40-41 36.60227540451207 39.0 36.0 41.0 30.0 41.0 42-43 36.43271663995219 39.0 36.0 41.0 30.0 41.0 44-45 36.418240652291445 39.0 36.0 41.0 30.0 41.0 46-47 36.24501230728178 39.5 36.0 41.0 29.5 41.0 48-49 36.05319605240767 39.0 35.5 41.0 29.0 41.0 50-51 34.91505108707278 38.0 34.0 40.0 26.5 40.5 52-53 35.038548604482514 38.5 34.5 39.5 27.5 40.5 54-55 35.52445100555105 39.0 35.0 40.5 28.0 41.0 56-57 35.40069069266639 39.0 35.0 41.0 27.0 41.0 58-59 35.17736783317228 38.5 35.0 40.5 27.0 41.0 60-61 34.87032311346847 38.0 34.5 40.0 26.0 41.0 62-63 34.50408876679823 37.5 34.0 40.0 26.0 41.0 64-65 34.11832834879539 37.0 34.0 40.0 25.5 41.0 66-67 33.741317366149815 36.5 34.0 39.5 24.5 41.0 68-69 33.32140934089057 36.0 33.0 39.0 23.0 41.0 70-71 32.90145647500484 35.5 33.0 39.0 22.5 40.0 72-73 32.46203340479655 35.0 32.5 37.5 21.5 40.0 74-75 32.016280087784715 35.0 32.0 37.0 20.0 39.0 76-77 31.5726148002736 35.0 32.0 36.5 18.5 39.0 78-79 31.152413289438527 35.0 31.0 36.0 17.5 38.5 80-81 30.702621008127547 35.0 31.0 36.0 13.0 37.0 82-83 30.27784318056769 34.0 30.5 35.0 7.5 37.0 84-85 29.864801100580095 34.0 30.0 35.0 4.5 36.5 86-87 29.53610828202696 34.0 30.0 35.0 2.0 36.0 88-89 29.12717922688144 34.0 29.5 35.0 2.0 36.0 90-91 28.73737837439497 34.0 29.0 35.0 2.0 35.0 92-93 28.322112272842716 34.0 29.0 35.0 2.0 35.0 94-95 27.84324176411846 34.0 28.0 35.0 2.0 35.0 96-97 27.275446044781084 34.0 27.0 35.0 2.0 35.0 98-99 26.5301997943947 33.0 25.0 35.0 2.0 35.0 100-101 23.98880307503785 31.0 12.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.33017456733512773 1101 2 0.1524531184515041 1101 3 0.3654843803823695 1101 4 0.4196181852947731 1101 5 0.38460650267334984 1101 6 0.3933865274885875 1101 7 0.41719698169422514 1101 8 0.34322300524198823 1101 9 0.48446904184358175 1101 10-11 0.48256043197095977 1101 12-13 0.4519156583549844 1101 14-15 0.4419550479718737 1101 16-17 0.5600573509955495 1101 18-19 0.5896479234437422 1101 20-21 0.5847807671080929 1101 22-23 0.5793407691013286 1101 24-25 0.5143518505608853 1101 26-27 0.4743957902351781 1101 28-29 0.5506592938905683 1101 30-31 0.5808051783262584 1101 32-33 0.5836783930677001 1101 34-35 0.5252810641180687 1101 36-37 0.5451001137307117 1101 38-39 0.6783465279843455 1101 40-41 0.6508218652526736 1101 42-43 0.4454661400615052 1101 44-45 0.7574840982389119 1101 46-47 0.7083145999131446 1101 48-49 0.6371565183309045 1101 50-51 0.7406374780006857 1101 52-53 0.7693484528863621 1101 54-55 0.6473817971583813 1101 56-57 0.7110714011328412 1101 58-59 0.6868763192453287 1101 60-61 0.8072954438973525 1101 62-63 0.8529076424337987 1101 64-65 0.774443780584285 1101 66-67 0.7330508656263461 1101 68-69 0.6634850506343142 1101 70-71 0.6655695508344266 1101 72-73 0.6658990111072143 1101 74-75 0.5922572188154334 1101 76-77 0.44746078319785454 1101 78-79 0.3025496425564391 1101 80-81 0.3452357761963327 1101 82-83 0.26603688348511056 1101 84-85 0.3094085518694847 1101 86-87 0.32875517858379055 1101 88-89 0.25482390724463855 1101 90-91 0.40268036233289095 1101 92-93 0.35795765103284793 1101 94-95 0.27865023939488864 1101 96-97 0.23819226305258923 1101 98-99 0.12069582662372369 1101 100-101 0.1948316297776067 1102 1 0.27209776241961947 1102 2 0.358478238176815 1102 3 0.3896716036587762 1102 4 0.3876261368625009 1102 5 0.29052355336459357 1102 6 0.30195651032043 1102 7 0.3167925360449999 1102 8 0.37731715903253615 1102 9 0.39704227758949173 1102 10-11 0.37860344272364443 1102 12-13 0.4160587865740055 1102 14-15 0.5377508372553308 1102 16-17 0.41336935966998567 1102 18-19 0.3479546009534502 1102 20-21 0.3748738365939559 1102 22-23 0.41060777913114777 1102 24-25 0.417131646350164 1102 26-27 0.38352816576603743 1102 28-29 0.4073771035951026 1102 30-31 0.41434525242041076 1102 32-33 0.3534385810138403 1102 34-35 0.30684246287111705 1102 36-37 0.41419020137868046 1102 38-39 0.45182913392994806 1102 40-41 0.38335317455059226 1102 42-43 0.3724103887287171 1102 44-45 0.5098964954558625 1102 46-47 0.4768488006902274 1102 48-49 0.4439695661306686 1102 50-51 0.37058525065814507 1102 52-53 0.3800418657353646 1102 54-55 0.4478469638702265 1102 56-57 0.5037180081627497 1102 58-59 0.31702830261932036 1102 60-61 0.39446125215614103 1102 62-63 0.4839729717913457 1102 64-65 0.4517243444227219 1102 66-67 0.5358429502021167 1102 68-69 0.32800804692961094 1102 70-71 0.40520124867485663 1102 72-73 0.421044217576906 1102 74-75 0.2705573904970109 1102 76-77 0.3191340460513814 1102 78-79 0.2720868251714208 1102 80-81 0.16978804871824238 1102 82-83 0.09057036139240537 1102 84-85 0.035374396271762976 1102 86-87 0.06645898268607553 1102 88-89 -0.11555216576520877 1102 90-91 -0.2621390105775596 1102 92-93 -0.13223372874486472 1102 94-95 -0.08493338760429836 1102 96-97 -0.27325654873018834 1102 98-99 -0.2618508436572924 1102 100-101 -0.44571485978596215 1103 1 0.1490810314028863 1103 2 0.2577317774303509 1103 3 0.20243865642583359 1103 4 0.21251159374795492 1103 5 0.2624617773028177 1103 6 0.20959614996006337 1103 7 0.2390010302534975 1103 8 0.31319875491413285 1103 9 0.26217998672720455 1103 10-11 0.24385698197718142 1103 12-13 0.2790163155315426 1103 14-15 0.3982630637675584 1103 16-17 0.40619561449624086 1103 18-19 0.4144539574528139 1103 20-21 0.44924964096976083 1103 22-23 0.36113287565624574 1103 24-25 0.3491342203527452 1103 26-27 0.30919354543141253 1103 28-29 0.2386821229001228 1103 30-31 0.2659179679931256 1103 32-33 0.2216290572043178 1103 34-35 0.07274979877845311 1103 36-37 0.20451195170041814 1103 38-39 0.39900802710884165 1103 40-41 0.4818164950139092 1103 42-43 0.22568966800799473 1103 44-45 0.31795569751506747 1103 46-47 0.28333786117929094 1103 48-49 0.327495949657056 1103 50-51 0.22876800484089443 1103 52-53 0.16348588117938334 1103 54-55 0.3333836434069113 1103 56-57 0.2706806851254271 1103 58-59 0.2575019190929382 1103 60-61 0.1488576485525357 1103 62-63 0.08204246986084485 1103 64-65 0.2591451938435725 1103 66-67 0.10958812394728312 1103 68-69 0.00515605207761638 1103 70-71 0.00954616501977057 1103 72-73 0.0283132008458864 1103 74-75 -0.047252133312511546 1103 76-77 0.013345114262449442 1103 78-79 0.004637661722259878 1103 80-81 0.006055744985939526 1103 82-83 -0.03481187798983498 1103 84-85 0.038548141445506445 1103 86-87 -0.11907254884545537 1103 88-89 -0.0015727577858015707 1103 90-91 -0.035709152147703094 1103 92-93 0.01987437936324099 1103 94-95 -0.1852808779517865 1103 96-97 -0.2752205126941547 1103 98-99 -0.36833218213863006 1103 100-101 -0.5512901493612468 1104 1 0.20129919473215807 1104 2 0.3291457599554448 1104 3 0.2970715288364829 1104 4 0.29845121302090405 1104 5 0.1190163116351286 1104 6 0.14887265590324006 1104 7 0.2400680424316164 1104 8 0.27592439541755454 1104 9 0.3216115683810017 1104 10-11 0.22987467355043378 1104 12-13 0.29788359550993704 1104 14-15 0.28985516091521646 1104 16-17 0.2832570193354229 1104 18-19 0.27355772211213036 1104 20-21 0.30461676855910724 1104 22-23 0.2980506736851609 1104 24-25 0.369260768257071 1104 26-27 0.33081350594026304 1104 28-29 0.276226015999562 1104 30-31 0.36420875517280393 1104 32-33 0.3894554694751733 1104 34-35 0.4282833265342063 1104 36-37 0.5109536025865182 1104 38-39 0.5843364291039137 1104 40-41 0.5185361150668655 1104 42-43 0.4564392487575688 1104 44-45 0.6783544079137798 1104 46-47 0.7028044417569888 1104 48-49 0.5185032628865898 1104 50-51 0.6465001670174999 1104 52-53 0.622073184211132 1104 54-55 0.7240596729718263 1104 56-57 0.7058151702599105 1104 58-59 0.7260210876121036 1104 60-61 0.7446770610386153 1104 62-63 0.765748398011219 1104 64-65 0.8611726903155059 1104 66-67 0.8215839581653412 1104 68-69 0.8441745799850295 1104 70-71 0.7663827996341794 1104 72-73 0.6714083883855153 1104 74-75 0.6289410151028605 1104 76-77 0.6681712968664044 1104 78-79 0.5592898608189039 1104 80-81 0.462067456553207 1104 82-83 0.3490816114592086 1104 84-85 0.49316863495489116 1104 86-87 0.45382235182722397 1104 88-89 0.4378397427378111 1104 90-91 0.35773956352323566 1104 92-93 0.37399170014722927 1104 94-95 0.30384540561894013 1104 96-97 0.33067333208857974 1104 98-99 0.35919303427547433 1104 100-101 0.24236295540296737 1105 1 0.3134168171779521 1105 2 0.31950986522043223 1105 3 0.3242093347932098 1105 4 0.23844544149589808 1105 5 0.28887694809580466 1105 6 0.299463185554238 1105 7 0.3149681625174807 1105 8 0.32574151828148956 1105 9 0.4169781667877501 1105 10-11 0.3919972458220897 1105 12-13 0.423977184517895 1105 14-15 0.4164374225341234 1105 16-17 0.4391183423215139 1105 18-19 0.4815588702473761 1105 20-21 0.47762888763985956 1105 22-23 0.5698390920461236 1105 24-25 0.4251726972532879 1105 26-27 0.47006325536409577 1105 28-29 0.42767652531281186 1105 30-31 0.5138295816843552 1105 32-33 0.3669064185236621 1105 34-35 0.5280075945909672 1105 36-37 0.6255563040416305 1105 38-39 0.7887876546695765 1105 40-41 0.8423876573092102 1105 42-43 0.6470011647692644 1105 44-45 0.7231053910080831 1105 46-47 0.7963852467529122 1105 48-49 0.6957329701429487 1105 50-51 0.7229003788083546 1105 52-53 0.6049437719251287 1105 54-55 0.7337088186571208 1105 56-57 0.7864270108042817 1105 58-59 0.7756913282493656 1105 60-61 0.7670129049011933 1105 62-63 0.6791057625388319 1105 64-65 0.6784007205203864 1105 66-67 0.7160207184608396 1105 68-69 0.7009361543044932 1105 70-71 0.6038155843641562 1105 72-73 0.689082912515147 1105 74-75 0.7324651509025202 1105 76-77 0.5992712631571138 1105 78-79 0.49821461841765213 1105 80-81 0.4552492466906841 1105 82-83 0.41096894781347615 1105 84-85 0.44247484604687415 1105 86-87 0.4333431834428403 1105 88-89 0.5458968954184833 1105 90-91 0.6913039822117568 1105 92-93 0.8136296252923998 1105 94-95 0.7634302877009063 1105 96-97 0.6040750711741438 1105 98-99 0.4770153660949745 1105 100-101 0.22419218669080365 1106 1 0.04418587626582138 1106 2 0.13695520842155418 1106 3 0.12002267672663436 1106 4 0.22076871045315727 1106 5 0.21625230024108788 1106 6 0.15766629464353343 1106 7 0.22349422140838726 1106 8 0.13422218676748798 1106 9 0.15651047543803998 1106 10-11 0.2229448395726692 1106 12-13 0.20250974090438234 1106 14-15 0.18623769638305987 1106 16-17 0.24814483949347022 1106 18-19 0.2907695654208169 1106 20-21 0.25222758568570214 1106 22-23 0.24763452164803113 1106 24-25 0.31976896536411203 1106 26-27 0.22096931982348877 1106 28-29 0.3320518869434963 1106 30-31 0.264682360542416 1106 32-33 0.2567611748076004 1106 34-35 0.2958009401957966 1106 36-37 0.24961083334563483 1106 38-39 0.468916840169868 1106 40-41 0.4975087383764176 1106 42-43 0.38558932788904343 1106 44-45 0.5246112951085067 1106 46-47 0.3661516521620456 1106 48-49 0.38974375224724866 1106 50-51 0.4448104974371674 1106 52-53 0.34956241440887936 1106 54-55 0.3783272658533221 1106 56-57 0.4292603903556653 1106 58-59 0.4909423508586386 1106 60-61 0.5510939988908632 1106 62-63 0.4651362446926086 1106 64-65 0.33954251657434753 1106 66-67 0.2886474586820924 1106 68-69 0.33325099799913005 1106 70-71 0.3150773009193415 1106 72-73 0.23492317623144743 1106 74-75 0.14663111406905927 1106 76-77 0.19188205675547465 1106 78-79 0.32819811940999344 1106 80-81 0.32054708251412123 1106 82-83 0.3659319436136812 1106 84-85 0.2194815790034319 1106 86-87 0.3536688644802517 1106 88-89 0.2808504783590564 1106 90-91 0.4369134997455113 1106 92-93 0.4243559889434749 1106 94-95 0.34668764488015213 1106 96-97 0.4258648647775267 1106 98-99 0.33623808540149014 1106 100-101 0.1380246266301164 1107 1 0.08098248789450224 1107 2 0.03406501933736905 1107 3 0.056775428795390326 1107 4 0.045764049951230845 1107 5 0.13893989476454038 1107 6 0.05023233649789205 1107 7 0.1600670611555941 1107 8 0.08986464797347082 1107 9 0.18080704469851838 1107 10-11 0.1336562660387628 1107 12-13 0.21288730595991012 1107 14-15 0.23208130824154694 1107 16-17 0.18159314317245645 1107 18-19 0.1327789569253568 1107 20-21 0.1558418303488338 1107 22-23 0.18176885121025776 1107 24-25 0.08401793310530792 1107 26-27 0.05738400755630835 1107 28-29 0.11724335392036522 1107 30-31 0.06897653006808468 1107 32-33 0.1411190389893875 1107 34-35 0.12616070382869538 1107 36-37 0.23709418264331106 1107 38-39 0.41029179331063403 1107 40-41 0.25743696325732657 1107 42-43 0.2217811927880362 1107 44-45 0.20414786157608233 1107 46-47 0.2207280616842482 1107 48-49 0.3059263707104307 1107 50-51 0.17005753793370815 1107 52-53 0.16323832683346495 1107 54-55 0.22919174659205765 1107 56-57 0.34015308492568863 1107 58-59 0.2700681152656941 1107 60-61 0.21333817122977905 1107 62-63 0.3592787225069358 1107 64-65 0.44749431579924703 1107 66-67 0.1993089923566771 1107 68-69 0.26071582730951803 1107 70-71 0.15487181854214782 1107 72-73 0.20184879733230332 1107 74-75 0.3553109684309206 1107 76-77 0.12539686156009822 1107 78-79 0.06670769428409429 1107 80-81 -0.015400605978605597 1107 82-83 0.01962370513427203 1107 84-85 -0.0413250572801509 1107 86-87 0.0013608822437127799 1107 88-89 -0.15697605335303066 1107 90-91 -0.013108565612689915 1107 92-93 0.08920577164545307 1107 94-95 -0.09858938552259033 1107 96-97 -0.0717951904163705 1107 98-99 0.029269635135317884 1107 100-101 -0.17082232790982133 1108 1 -0.04835218898951865 1108 2 0.0516172080504802 1108 3 0.0626939554566377 1108 4 0.09887200663897744 1108 5 0.14909206648412976 1108 6 0.009363701768435817 1108 7 0.08130857103042644 1108 8 0.06610238740960739 1108 9 0.1015602562095097 1108 10-11 0.09791005108127138 1108 12-13 0.02352817432911536 1108 14-15 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-0.7910965906192793 2316 84-85 -0.7353707570592185 2316 86-87 -0.6272350267470941 2316 88-89 -0.889345542403845 2316 90-91 -0.7045641746509439 2316 92-93 -0.7651661019761491 2316 94-95 -0.7941654738120612 2316 96-97 -0.7698890747049774 2316 98-99 -0.8045994882684688 2316 100-101 -0.3956239186509585 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8185.0 3 2372.0 4 2251.0 5 2458.0 6 2776.0 7 3383.0 8 4050.0 9 4983.0 10 5927.0 11 7468.0 12 9636.0 13 13914.0 14 12666.0 15 13302.0 16 16460.0 17 21084.0 18 18321.0 19 14201.0 20 13988.0 21 15275.0 22 16224.0 23 17650.0 24 20605.0 25 23586.0 26 27139.0 27 32153.0 28 38484.0 29 46570.0 30 56777.0 31 70477.0 32 90260.0 33 119441.0 34 165068.0 35 243327.0 36 382405.0 37 582354.0 38 515215.0 39 80288.0 40 24.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.268201639938795 18.573994793002694 14.499430945367806 23.658372621690713 2 15.453758082521004 19.579477111389593 40.340527362649574 24.62623744343983 3 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22.517620093029826 22-23 23.107291360336546 25.514970243793258 28.274088513289463 23.103649882580733 24-25 23.029511031000837 25.784014934843984 28.259060686598186 22.927413347556993 26-27 22.69988175305646 26.070296695173578 28.297841777165665 22.931979774604294 28-29 22.664063620747367 26.284994905484787 28.461290880266017 22.589650593501826 30-31 22.757352089239152 25.904075341420235 28.23499099023547 23.103581579105146 32-33 22.62912169150262 25.961268273880545 28.206434347907322 23.203175686709518 34-35 23.195450448716333 26.135108366637926 27.90838564719279 22.761055537452954 36-37 23.175568960162646 25.78888333983728 28.033943966709625 23.001603733290448 38-39 22.780136195146056 26.03405155208446 28.174050279921865 23.011761972847626 40-41 22.979111859053948 25.980407609181555 28.089573220942814 22.95090731082168 42-43 22.9469243484586 25.92860494681925 27.731228167866234 23.393242536855915 44-45 22.92141014262192 26.20602386332224 27.715465758550152 23.157100235505688 46-47 23.035753771035928 26.4706224069604 27.412023607783127 23.08160021422055 48-49 23.188402322250422 26.205251142478897 27.49066669715982 23.11567983811086 50-51 22.999256588121234 26.571856908612716 27.357575580976107 23.071310922289946 52-53 23.210337697325762 26.57967575096104 27.08020578903378 23.12978076267942 54-55 22.962630633461416 26.33113639355943 27.285129000600776 23.42110397237838 56-57 22.95344846880467 26.61099108230748 27.31867656583202 23.11688388305583 58-59 23.087289716800868 26.238989291321978 27.36169171084837 23.31202928102879 60-61 23.126403471128317 26.372626317698973 27.572828485716954 22.92814172545576 62-63 22.862767563728433 26.524669121775318 27.47729749732692 23.135265817169326 64-65 22.74006831837966 26.28677009653508 27.412874331400396 23.560287253684873 66-67 22.785368125458223 26.502229456492167 27.274290009176045 23.43811240887356 68-69 22.745119668583683 26.36696963732617 27.421627024936097 23.46628366915405 70-71 22.85379982331937 26.401284171920693 27.31161921395382 23.433296790806114 72-73 22.985589948929437 26.292210678524725 27.286749503403783 23.435449869142055 74-75 22.82916293554935 26.710994338538608 26.97854657494958 23.481296150962468 76-77 22.911098136571738 26.47303334608248 27.052092857540664 23.56377565980512 78-79 22.959190258260236 26.53503459043312 27.141367399775135 23.364407751531505 80-81 22.837092153299306 26.72555012416523 27.120751219481615 23.316606503053844 82-83 23.012576990410246 26.604706773533405 26.96778471561722 23.41493152043913 84-85 22.90504657992794 26.529222777785773 26.96985019700438 23.595880445281907 86-87 22.90524405827674 26.43767667510589 27.07839995279444 23.57867931382293 88-89 22.90106815278801 26.31811322884304 27.05693827184173 23.72388034652722 90-91 22.774576667633273 26.53064838529019 27.126881282470734 23.567893664605805 92-93 22.969355341210576 26.26848943725558 27.122256017085295 23.63989920444855 94-95 22.87129216927696 26.142812030330376 27.218943424569908 23.766952375822765 96-97 22.902721379691744 26.113177238967378 27.332740949748175 23.6513604315927 98-99 22.802941572490727 26.221698800536373 26.899341337135418 24.076018289837485 100-101 22.890613344869866 25.666812901812413 27.39354938884589 24.04902436447183 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2340.0 1 1389.5 2 544.5 3 690.5 4 607.5 5 568.0 6 995.0 7 2236.5 8 3638.5 9 4050.0 10 3359.5 11 2692.5 12 2774.5 13 3010.5 14 3233.5 15 3552.5 16 3741.0 17 3777.0 18 3952.5 19 4136.5 20 4391.5 21 4870.5 22 5647.5 23 6944.0 24 8481.5 25 10563.0 26 13374.0 27 16321.5 28 18868.5 29 22182.5 30 25990.5 31 29860.5 32 34504.5 33 38603.5 34 42614.5 35 46611.0 36 51842.5 37 57924.5 38 61838.0 39 63877.0 40 67033.5 41 73866.0 42 79987.0 43 84889.0 44 90547.5 45 95481.0 46 96735.5 47 99894.0 48 109758.5 49 112183.5 50 106877.0 51 103589.0 52 102063.0 53 102560.5 54 102247.0 55 96584.5 56 87060.5 57 79633.5 58 73827.5 59 65070.5 60 55238.5 61 45796.5 62 37470.5 63 29999.0 64 23171.0 65 17750.5 66 13628.0 67 11441.0 68 9636.0 69 7307.5 70 5458.0 71 4272.5 72 3496.5 73 2935.0 74 2291.0 75 1797.0 76 1302.5 77 771.0 78 484.5 79 322.0 80 215.0 81 170.5 82 121.5 83 75.0 84 55.0 85 44.5 86 39.0 87 30.5 88 20.0 89 14.0 90 11.5 91 11.0 92 10.0 93 8.5 94 7.5 95 8.0 96 6.5 97 4.0 98 3.5 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.274740723779168 2 0.10456687078952949 3 0.11478465289128316 4 0.11632834659010927 5 0.1233484774585803 6 0.05270611343134808 7 0.06086563726800029 8 0.06582750987136989 9 0.06270336786184089 10-11 0.058439832884130724 12-13 0.06246446288464161 14-15 0.0583663236603771 16-17 0.05992839466514159 18-19 0.06411842041909813 20-21 0.06740795818207279 22-23 0.07610042389093877 24-25 0.08350647818411634 26-27 0.08392915622069969 28-29 0.07854460558074676 30-31 0.0746118621099279 32-33 0.06759173124145684 34-35 0.23087409450419316 36-37 0.35185189949671913 38-39 0.32452484556630956 40-41 0.30941870008493993 42-43 0.33095690264475164 44-45 0.34356373451849803 46-47 0.3499957915969401 48-49 0.35857799347017566 50-51 0.3527891420995778 52-53 0.35361612086680605 54-55 0.33990665063675524 56-57 0.3185338438303892 58-59 0.30410765866874057 60-61 0.26325490756766434 62-63 0.330295319630969 64-65 0.3588536530592517 66-67 0.36330096109634596 68-69 0.37484190922566485 70-71 0.37513594612067935 72-73 0.3599011594977409 74-75 0.36293341497757786 76-77 0.3474781006833785 78-79 0.3260134073473204 80-81 0.32478212784944727 82-83 0.3384364661616828 84-85 0.3449787870757553 86-87 0.3382159384904219 88-89 0.35350585703117565 90-91 0.3759629248879076 92-93 0.37164425799238227 94-95 0.3699351685401105 96-97 0.3759445475819692 98-99 0.36578189739803074 100-101 0.3440415444728966 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2720747.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.00216000405533 #Duplication Level Percentage of deduplicated Percentage of total 1 72.106757772464 33.89173366195013 2 14.294376613022235 13.437331534469955 3 5.333657542674461 7.520802756828646 4 2.5508970492759206 4.795906850557578 5 1.4267825105102 3.353092992499409 6 0.9065982493683374 2.5567245585724225 7 0.6118495245233111 2.013077447503489 8 0.4310695572902705 1.620896024370767 9 0.32070949205251875 1.3566634974244618 >10 1.791046315772664 15.59434031787405 >50 0.14768054053758803 4.815410006165498 >100 0.07378694424428836 6.325929792966675 >500 0.0035320487196262838 1.11945030222439 >1k 0.0010203696301077023 0.8193709205207057 >5k 2.3546991464023896E-4 0.7792693360719886 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9233 0.3393553314586031 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6766 0.24868170395850844 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5124 0.18833063125678354 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2994 0.11004330795917446 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.8377305938405886E-5 0.0 12-13 0.0 0.0 0.0 3.675461187681177E-5 0.0 14-15 0.0 0.0 0.0 3.675461187681177E-5 0.0 16-17 0.0 0.0 0.0 3.675461187681177E-5 0.0 18-19 0.0 0.0 0.0 5.513191781521766E-5 0.0 20-21 0.0 0.0 0.0 9.188652969202942E-5 0.0 22-23 0.0 0.0 0.0 1.470184475072471E-4 0.0 24-25 0.0 0.0 0.0 3.3079150689130593E-4 0.0 26-27 0.0 0.0 0.0 3.675461187681177E-4 0.0 28-29 0.0 0.0 0.0 0.0012129021919347885 0.0 30-31 0.0 0.0 0.0 0.0038776115530036416 0.0 32-33 0.0 0.0 0.0 0.009390803334525409 0.0 34-35 0.0 0.0 0.0 0.018064891737452987 0.0 36-37 0.0 0.0 0.0 0.033814242926666835 0.0 38-39 0.0 0.0 0.0 0.056436706536844475 0.0 40-41 0.0 0.0 0.0 0.08181576603778301 0.0 42-43 0.0 0.0 0.0 0.10963900722852951 0.0 44-45 0.0 0.0 0.0 0.14560339494998986 0.0 46-47 0.0 0.0 0.0 0.18928625116558062 0.0 48-49 0.0 0.0 0.0 0.23975033327244322 0.0 50-51 0.0 0.0 0.0 0.2930812751056971 0.0 52-53 0.0 0.0 0.0 0.3494260951128495 0.0 54-55 0.0 0.0 0.0 0.4137650432032085 0.0 56-57 0.0 0.0 0.0 0.4933204006105676 0.0 58-59 0.0 0.0 0.0 0.5844718380650609 0.0 60-61 0.0 0.0 0.0 0.6884690123704997 0.0 62-63 0.0 0.0 0.0 0.7931461469956597 0.0 64-65 0.0 0.0 0.0 0.9088864197957399 0.0 66-67 0.0 0.0 0.0 1.0478556073019654 0.0 68-69 0.0 0.0 0.0 1.203143842481495 0.0 70-71 0.0 0.0 0.0 1.3786103595813943 0.0 72-73 0.0 0.0 0.0 1.5621261366823156 0.0 74-75 0.0 0.0 0.0 1.7594984024607947 0.0 76-77 0.0 0.0 0.0 1.9787947942237922 0.0 78-79 0.0 0.0 0.0 2.2307292813333985 0.0 80-81 0.0 0.0 0.0 2.5119388168028856 0.0 82-83 0.0 0.0 0.0 2.8017856860634227 0.0 84-85 0.0 0.0 0.0 3.1031918807592183 0.0 86-87 0.0 0.0 0.0 3.4235267005715713 0.0 88-89 0.0 0.0 0.0 3.7800280584707067 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 11085 0.0 32.775986 1 GGTATCA 7465 0.0 29.456501 1 GTACATG 9740 0.0 26.67217 1 ATCAACG 13590 0.0 26.555552 3 TCAACGC 13715 0.0 26.313524 4 CAACGCA 13805 0.0 26.073273 5 TATCAAC 14060 0.0 25.735308 2 AACGCAG 14140 0.0 25.505733 6 TACATGG 9930 0.0 25.26368 2 ACATGGG 9810 0.0 24.460855 3 GTCCCGA 375 0.0 22.74803 6 ACGCAGA 15850 0.0 22.365307 7 CGCAGAG 15995 0.0 22.132929 8 GAGTACT 8165 0.0 21.011782 12-13 CATGGGG 6015 0.0 20.656456 4 AGTACTT 8520 0.0 20.470041 12-13 GCAGAGT 17160 0.0 20.408998 9 AGAGTAC 13365 0.0 20.282879 10-11 GTACTTT 8595 0.0 19.878056 14-15 TAGCGCG 200 1.30387125E-5 18.96925 4 >>END_MODULE