FastQCFastQC Report
Fri 27 May 2016
SRR939242_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939242_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2720747
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94480.3472575730121176No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74860.27514502450981293No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55360.20347353135002996No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT32910.12095942768658756No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86600.030.2204151
GTATCAA122900.029.5799271
CGCTATA1354.1643034E-728.1975821
ATCAACG153300.023.5266593
GTACATG103400.023.423561
TCAACGC155150.023.2461264
CAACGCA156100.023.1350545
TATCAAC157700.022.990612
TACATGG102750.022.7695222
AACGCAG159850.022.592326
ACATGGG99900.022.089013
GAGTACT89250.021.58890312-13
AGTACTT93300.020.82979612-13
AGAGTAC142100.020.74007410-11
GGACCGA5500.020.7079646
TATACCG1150.003744735120.6329335
ACGCAGA176600.020.26147
GTACTTT94650.020.25692414-15
CATGGGG63100.019.929914
CGCAGAG180100.019.7885998
Produced by FastQC (version 0.11.5)