##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939242_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2720747 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.131248146189265 33.0 31.0 34.0 30.0 34.0 2 32.35952111681093 34.0 31.0 34.0 30.0 34.0 3 32.44510349547385 34.0 31.0 34.0 30.0 34.0 4 36.01923920158692 37.0 35.0 37.0 35.0 37.0 5 35.94785843740708 37.0 35.0 37.0 35.0 37.0 6 35.98563960559361 37.0 35.0 37.0 35.0 37.0 7 35.92684619334322 37.0 35.0 37.0 35.0 37.0 8 35.9025425737858 37.0 35.0 37.0 35.0 37.0 9 37.58707406458594 39.0 37.0 39.0 35.0 39.0 10-11 37.487793058303474 39.0 37.0 39.0 34.5 39.0 12-13 37.395781746704124 39.0 37.0 39.0 34.0 39.0 14-15 38.63846105499703 40.0 38.0 41.0 34.0 41.0 16-17 38.56739509406792 40.0 38.0 41.0 34.0 41.0 18-19 38.690944619253465 40.0 38.0 41.0 34.0 41.0 20-21 38.69013473138076 40.0 38.0 41.0 34.0 41.0 22-23 38.61961347379966 40.0 38.0 41.0 34.0 41.0 24-25 38.543164248642015 40.0 38.0 41.0 34.0 41.0 26-27 38.356413881922876 40.0 38.0 41.0 34.0 41.0 28-29 38.18377985898725 40.0 38.0 41.0 33.0 41.0 30-31 37.98087584034826 40.0 37.0 41.0 33.0 41.0 32-33 37.76391869585816 40.0 37.0 41.0 33.0 41.0 34-35 37.7161512996247 40.0 37.0 41.0 33.0 41.0 36-37 37.62898810510496 40.0 37.0 41.0 32.5 41.0 38-39 37.509364523787035 40.0 37.0 41.0 32.0 41.0 40-41 37.319032787686616 40.0 36.5 41.0 31.5 41.0 42-43 37.124014287252734 40.0 36.0 41.0 31.0 41.0 44-45 36.88458592438033 39.5 36.0 41.0 31.0 41.0 46-47 36.59412378291697 39.0 35.5 41.0 30.0 41.0 48-49 36.2596933856768 39.0 35.0 41.0 30.0 41.0 50-51 35.98334556649332 39.0 35.0 40.0 29.0 41.0 52-53 35.726009621622296 39.0 35.0 40.0 28.0 41.0 54-55 35.40397710628736 38.0 34.5 40.0 28.0 41.0 56-57 35.117555950626794 38.0 34.0 40.0 27.0 41.0 58-59 34.781177558957154 38.0 34.0 40.0 26.0 41.0 60-61 34.40912900023413 37.0 34.0 40.0 26.0 41.0 62-63 33.97795587020771 36.5 33.0 39.5 25.5 41.0 64-65 33.50965286371721 36.0 32.5 39.0 24.5 40.0 66-67 33.095578346682 35.5 32.0 39.0 23.0 40.0 68-69 32.6677017377948 35.0 32.0 38.0 22.5 40.0 70-71 32.22493418167878 35.0 31.0 37.5 21.5 40.0 72-73 31.680357085756228 35.0 31.0 37.0 20.0 39.0 74-75 31.13596321157388 34.5 30.5 36.5 18.0 39.0 76-77 29.20798277090814 32.5 28.0 34.5 13.0 36.5 78-79 30.212677253710105 34.0 29.5 35.0 15.5 37.0 80-81 30.256601771498783 34.0 30.0 35.0 14.0 37.0 82-83 29.922858317954592 34.0 30.0 35.0 9.5 37.0 84-85 29.50523385673126 34.0 29.5 35.0 7.0 36.0 86-87 28.90348257298455 34.0 29.0 35.0 2.0 36.0 88-89 28.346204737154906 33.0 28.5 35.0 2.0 35.0 90-91 27.85763634031389 33.0 27.0 35.0 2.0 35.0 92-93 27.413987224832006 33.0 26.0 35.0 2.0 35.0 94-95 26.890317989875577 33.0 25.5 35.0 2.0 35.0 96-97 26.189971724677083 32.0 24.5 34.0 2.0 35.0 98-99 25.25355922472762 32.0 21.0 34.0 2.0 35.0 100-101 21.753688600961425 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.23182602760412863 1101 2 0.20974815840238392 1101 3 0.24258625616268148 1101 4 0.1892814200600057 1101 5 0.24354616597181433 1101 6 0.14660975141107713 1101 7 0.12132709561010557 1101 8 0.16280878371699004 1101 9 0.20228834817145724 1101 10-11 0.23680151057259735 1101 12-13 0.3104820951498368 1101 14-15 0.30255902719351724 1101 16-17 0.32024103606834586 1101 18-19 0.2941685575530144 1101 20-21 0.30632250597732735 1101 22-23 0.3532359284959625 1101 24-25 0.4419087984836878 1101 26-27 0.3815176461481258 1101 28-29 0.25953811784528824 1101 30-31 0.23789101054853035 1101 32-33 0.17425937071050868 1101 34-35 0.2464562378603361 1101 36-37 0.33760779037468325 1101 38-39 0.3649545080807499 1101 40-41 0.37212232567163994 1101 42-43 0.4522866861514885 1101 44-45 0.5898002501954309 1101 46-47 0.446087088319004 1101 48-49 0.44524953868516093 1101 50-51 0.34414562156852213 1101 52-53 0.32279309345792484 1101 54-55 0.3162457470061355 1101 56-57 0.4145965483854255 1101 58-59 0.28869409345384867 1101 60-61 0.3339812458557816 1101 62-63 0.2608460733671194 1101 64-65 0.190464238097654 1101 66-67 0.2993241783119345 1101 68-69 0.4403916588188679 1101 70-71 0.32525584964359666 1101 72-73 0.022097611666218597 1101 74-75 -0.19827455887060808 1101 76-77 0.02010207693065169 1101 78-79 0.24678549357025048 1101 80-81 0.43692383607674046 1101 82-83 0.3930296975700962 1101 84-85 0.42863942092737517 1101 86-87 0.48805839100878856 1101 88-89 0.5724982308346185 1101 90-91 0.605258261551505 1101 92-93 0.5119119454539707 1101 94-95 0.5465650613908437 1101 96-97 0.31400976430270333 1101 98-99 0.33591638118484823 1101 100-101 0.34136470095215543 1102 1 0.1954765200591737 1102 2 0.20885324559855434 1102 3 0.2527975148597079 1102 4 0.16490046767905397 1102 5 0.14940755568899533 1102 6 0.17186609911144757 1102 7 0.20417303398545528 1102 8 0.22551340104779172 1102 9 0.3881367262323536 1102 10-11 0.29040989586220434 1102 12-13 0.28990126927109827 1102 14-15 0.306500836175168 1102 16-17 0.35920191074080066 1102 18-19 0.31504576004690676 1102 20-21 0.3782632306542908 1102 22-23 0.29139224908871597 1102 24-25 0.30562073487114816 1102 26-27 0.3426059266216015 1102 28-29 0.32500084487158176 1102 30-31 0.31947554113495613 1102 32-33 0.3041073873365079 1102 34-35 0.411248864588444 1102 36-37 0.5110224465597426 1102 38-39 0.3918951423989867 1102 40-41 0.5348970613723694 1102 42-43 0.5493196670857969 1102 44-45 0.5957581430299825 1102 46-47 0.5115216234233699 1102 48-49 0.46391909683099897 1102 50-51 0.4533679943886142 1102 52-53 0.6130488086472283 1102 54-55 0.529600766433262 1102 56-57 0.5021096955797972 1102 58-59 0.4667839100256401 1102 60-61 0.3786697793336842 1102 62-63 0.4189789006915987 1102 64-65 0.34145330469203117 1102 66-67 0.38988313737942804 1102 68-69 0.3931651294974614 1102 70-71 0.34123813933366876 1102 72-73 0.41140853603596383 1102 74-75 0.5296607442829107 1102 76-77 0.5906845292664258 1102 78-79 0.49039261383771304 1102 80-81 0.5220385012976649 1102 82-83 0.47215466368557557 1102 84-85 0.5643791878018689 1102 86-87 0.5085568097311146 1102 88-89 0.675973065929945 1102 90-91 0.7761461263748508 1102 92-93 0.6346480076207719 1102 94-95 0.5162132045190617 1102 96-97 0.18370814688153914 1102 98-99 0.1834609732152046 1102 100-101 0.23463008452494094 1103 1 0.12453700911966337 1103 2 0.1361685609138732 1103 3 0.1378065238687185 1103 4 0.16611539689397858 1103 5 0.10898541926685823 1103 6 0.02724319048853374 1103 7 0.19936479717721767 1103 8 0.09280812434251118 1103 9 0.13291922301485215 1103 10-11 0.18409844155075206 1103 12-13 0.1980840234538448 1103 14-15 0.19949440116874229 1103 16-17 0.12370641524530157 1103 18-19 0.2260650650662157 1103 20-21 0.24466220105326641 1103 22-23 0.2964681821646451 1103 24-25 0.17215870140911704 1103 26-27 0.18638456240024226 1103 28-29 0.13522478309551644 1103 30-31 0.15168146134087834 1103 32-33 0.14089245812158424 1103 34-35 0.27024500358458425 1103 36-37 0.3228242483615489 1103 38-39 0.3088681153719648 1103 40-41 0.3010901115654221 1103 42-43 0.30564656541270097 1103 44-45 0.5063553116271606 1103 46-47 0.356394210295953 1103 48-49 0.17151755242944944 1103 50-51 0.3586009416215603 1103 52-53 0.07661380221222203 1103 54-55 0.11962650645899942 1103 56-57 0.11942243689253473 1103 58-59 0.05903616227789854 1103 60-61 -0.11336110869720528 1103 62-63 -0.27894387923117847 1103 64-65 -0.014436013197290265 1103 66-67 -0.12047207497577972 1103 68-69 -0.241794973462639 1103 70-71 0.02758820368373449 1103 72-73 0.05655139317882174 1103 74-75 -0.07299047836831107 1103 76-77 0.04529714187903622 1103 78-79 0.15821758166268296 1103 80-81 0.07154429280345553 1103 82-83 0.12735929689020864 1103 84-85 -0.11270189327921543 1103 86-87 -0.039687720513416735 1103 88-89 0.3140219719788533 1103 90-91 0.3109003091290319 1103 92-93 0.1307612637340263 1103 94-95 0.07647575278161156 1103 96-97 -0.13258786341446793 1103 98-99 -0.32388265612842204 1103 100-101 -0.43558613169127725 1104 1 0.06212394670659904 1104 2 0.052444288300705466 1104 3 0.039871214822298384 1104 4 0.09964430820066639 1104 5 0.08560850700105505 1104 6 0.019063148975163813 1104 7 0.08436928218170436 1104 8 0.10322084586634617 1104 9 0.09236033801152388 1104 10-11 0.11579752880538763 1104 12-13 0.062069726328438435 1104 14-15 0.10502085113963489 1104 16-17 0.02363245961578997 1104 18-19 0.05183780750562761 1104 20-21 0.12191968942186548 1104 22-23 0.11634505759707281 1104 24-25 0.10519852333782609 1104 26-27 0.05530904243583734 1104 28-29 0.18022996143402992 1104 30-31 0.1972229624379338 1104 32-33 0.21708242875599382 1104 34-35 0.31289602401338357 1104 36-37 0.34418796416969855 1104 38-39 0.3551779172373202 1104 40-41 0.37257949416591174 1104 42-43 0.48756390844114605 1104 44-45 0.5263397260560083 1104 46-47 0.4335508041192142 1104 48-49 0.385156443846121 1104 50-51 0.31358326882611465 1104 52-53 0.07135248028423291 1104 54-55 0.15077013204707157 1104 56-57 0.2670680623159356 1104 58-59 0.2732261098011719 1104 60-61 0.26074214318382616 1104 62-63 0.40881653630701464 1104 64-65 0.22674708909692498 1104 66-67 0.23605234265974673 1104 68-69 0.30536907370140653 1104 70-71 0.11566986398761259 1104 72-73 0.223976971715512 1104 74-75 0.12310417328189516 1104 76-77 0.368714137518257 1104 78-79 0.5056777402339527 1104 80-81 0.5434263480188442 1104 82-83 0.5233433762076203 1104 84-85 0.4097826403164291 1104 86-87 0.31279773752759965 1104 88-89 0.5913304492873266 1104 90-91 0.7667223649510895 1104 92-93 0.7573694014532713 1104 94-95 0.5540696525977289 1104 96-97 0.6359920440543263 1104 98-99 0.5573689417898429 1104 100-101 0.18587566199273908 1105 1 0.09471118843926973 1105 2 0.12217401378588733 1105 3 0.08799291715855873 1105 4 0.029761180179946223 1105 5 0.042187737026893046 1105 6 0.06704336762004459 1105 7 0.09225927657844579 1105 8 0.1380791181892178 1105 9 0.03365582382641463 1105 10-11 0.13941296575582385 1105 12-13 0.12944064244165077 1105 14-15 0.2147232249807658 1105 16-17 0.13285401326096746 1105 18-19 0.23200635117991197 1105 20-21 0.17964089896299384 1105 22-23 0.18199187784576054 1105 24-25 0.28180585659600155 1105 26-27 0.18107640566779537 1105 28-29 0.2674763214190179 1105 30-31 0.1662545801868589 1105 32-33 0.18799308734364928 1105 34-35 0.25259711903265014 1105 36-37 0.19253490461642997 1105 38-39 0.1858790790021132 1105 40-41 0.32906155055635367 1105 42-43 0.24132409344404948 1105 44-45 0.3451187484415641 1105 46-47 0.3063282201250175 1105 48-49 0.3164545433934336 1105 50-51 0.23223077728287933 1105 52-53 0.20289424307287618 1105 54-55 0.133416715601534 1105 56-57 0.1403339406001365 1105 58-59 0.16271844275927094 1105 60-61 0.11467299201305536 1105 62-63 0.004127326478709392 1105 64-65 0.1720636423803299 1105 66-67 0.1440516959572804 1105 68-69 0.39376490104026374 1105 70-71 0.4052796185940366 1105 72-73 0.3600264413690155 1105 74-75 0.28624959271583705 1105 76-77 0.4545021918636998 1105 78-79 0.4768279675894078 1105 80-81 0.6515580986418499 1105 82-83 0.594277816394932 1105 84-85 0.47483667334481083 1105 86-87 0.5633704029345132 1105 88-89 0.5822055925237919 1105 90-91 0.7185955445229197 1105 92-93 0.6129984752441011 1105 94-95 0.6900144467550895 1105 96-97 0.38939873016132864 1105 98-99 0.5693665204415943 1105 100-101 0.6722449913572355 1106 1 -0.03599281199817739 1106 2 -0.036766333680866126 1106 3 0.06120044253617607 1106 4 0.05403765377790393 1106 5 0.01473784083617602 1106 6 0.060733627067918405 1106 7 0.034446909966810324 1106 8 -0.016989514223268998 1106 9 0.030412516191063332 1106 10-11 0.07507673974898665 1106 12-13 0.10550564306377908 1106 14-15 0.12332356288401769 1106 16-17 0.11513648107059993 1106 18-19 -0.007831373240342998 1106 20-21 0.0950493791293141 1106 22-23 0.06844479485580734 1106 24-25 0.08103498691361466 1106 26-27 0.13077145021846803 1106 28-29 0.11604491951669615 1106 30-31 0.15183636130969802 1106 32-33 0.05791198442656054 1106 34-35 0.10845126769334001 1106 36-37 0.12212209743609748 1106 38-39 0.10747789533640884 1106 40-41 0.22296894185512173 1106 42-43 0.18686048974161906 1106 44-45 0.17609528659600926 1106 46-47 0.08829720957893272 1106 48-49 -0.09994564876951273 1106 50-51 -0.05618388680301223 1106 52-53 -0.010363033201528538 1106 54-55 -0.09599690843869269 1106 56-57 -0.03205565079694139 1106 58-59 -0.09389838745605772 1106 60-61 -0.12414993463303148 1106 62-63 -0.07350937619712283 1106 64-65 -0.17578092714733629 1106 66-67 -0.1014312334005183 1106 68-69 -0.06327707015872619 1106 70-71 -0.20837208272315166 1106 72-73 -0.1526675977297849 1106 74-75 -0.1768815138559212 1106 76-77 -0.017616744946273855 1106 78-79 0.1758003087355462 1106 80-81 0.14886459518888628 1106 82-83 0.14213435425923748 1106 84-85 -0.09292657007999594 1106 86-87 -0.062296434283275914 1106 88-89 0.15123604287360592 1106 90-91 0.20915876216977125 1106 92-93 0.15807969812354372 1106 94-95 0.222784952086716 1106 96-97 0.35893610512848184 1106 98-99 0.3401278879900502 1106 100-101 0.24319853579826756 1107 1 0.019655546861152118 1107 2 0.036283898734126296 1107 3 0.029161813584664742 1107 4 -0.015454463364406479 1107 5 0.07387489891043231 1107 6 0.1392228516485261 1107 7 0.0673465963065567 1107 8 0.060223783561454525 1107 9 0.026196575308595982 1107 10-11 0.06505574545887072 1107 12-13 0.1309816752039552 1107 14-15 0.09934600921706505 1107 16-17 0.07780196278347518 1107 18-19 0.16417612694777262 1107 20-21 0.060497943118512865 1107 22-23 -0.0229985914004871 1107 24-25 0.08282604322399578 1107 26-27 0.00467826593984455 1107 28-29 0.045244404590548015 1107 30-31 0.10555828898819897 1107 32-33 0.02622240164824774 1107 34-35 0.03502195852546919 1107 36-37 0.11544086966669198 1107 38-39 0.05587952959648845 1107 40-41 -0.01444251708196731 1107 42-43 -0.13125696575312418 1107 44-45 -0.02259834929371607 1107 46-47 -0.15698110881707095 1107 48-49 -0.1054183999163314 1107 50-51 -0.17982378286873058 1107 52-53 -0.020425039908587905 1107 54-55 -0.16740677975461438 1107 56-57 -0.05696706736582513 1107 58-59 -0.0952197329616098 1107 60-61 -0.2592360234245774 1107 62-63 -0.17816499730474789 1107 64-65 -0.054457181906975904 1107 66-67 -0.2235865477623875 1107 68-69 -0.04625095168911031 1107 70-71 -0.07455785338036236 1107 72-73 -0.07875956180426158 1107 74-75 -0.06808580788986873 1107 76-77 0.09985861201427682 1107 78-79 0.3367105945819233 1107 80-81 0.19095445237755015 1107 82-83 0.18420150209307096 1107 84-85 0.1362948786683802 1107 86-87 0.1263491594578916 1107 88-89 0.30625565765515717 1107 90-91 0.26504438294524135 1107 92-93 0.34979763031137523 1107 94-95 0.3725795849510156 1107 96-97 0.4232845195070958 1107 98-99 0.4184846526323298 1107 100-101 0.351733389234802 1108 1 -0.12205506400302113 1108 2 -0.08092032097093238 1108 3 -0.03954553664660665 1108 4 -1.500668204670319E-4 1108 5 -0.016497206215767335 1108 6 -0.023997846466784267 1108 7 -0.08170840430414472 1108 8 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64-65 -0.5745323273471783 2316 66-67 -0.6215742371107282 2316 68-69 -0.3977519022429732 2316 70-71 -0.3843532130338616 2316 72-73 -0.4247517942928525 2316 74-75 -0.3530047138636867 2316 76-77 -0.44607153431515556 2316 78-79 -0.42972756676889645 2316 80-81 -0.4308608390517996 2316 82-83 -0.33915265289632757 2316 84-85 -0.5030493836267027 2316 86-87 -0.5690190332955574 2316 88-89 -0.6953033662502577 2316 90-91 -0.9818157716730518 2316 92-93 -0.5940026461550154 2316 94-95 -0.39823324293549334 2316 96-97 -0.21900283218755945 2316 98-99 -0.10107762389441532 2316 100-101 0.5385458909603074 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 9.0 4 26.0 5 78.0 6 184.0 7 398.0 8 896.0 9 1676.0 10 2840.0 11 4110.0 12 6067.0 13 8082.0 14 10464.0 15 13662.0 16 17341.0 17 23283.0 18 22194.0 19 17495.0 20 16927.0 21 18119.0 22 19542.0 23 21822.0 24 25170.0 25 30077.0 26 35108.0 27 42852.0 28 51277.0 29 62207.0 30 74566.0 31 90381.0 32 112019.0 33 141364.0 34 190251.0 35 268716.0 36 403630.0 37 581688.0 38 383334.0 39 22887.0 40 2.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.09923678188029 19.648757513331443 15.294913230465337 22.957092474322923 2 16.23487961210653 20.554391863705078 39.10473851482699 24.1059900093614 3 19.720245947160837 23.94041048285636 29.5890062545323 26.7503373154505 4 13.812089106410848 16.921124970458482 35.84107599861361 33.42570992451706 5 14.475509850787303 36.827165480656596 33.616098814038935 15.081225854517161 6 32.92290683404227 35.675496472108584 16.737462174910053 14.6641345189391 7 29.405600741267012 32.11294545211297 20.023287722085147 18.45816608453487 8 26.346330621700588 35.54468680843901 19.025436764241586 19.083545805618822 9 26.252606361414717 17.043664846455773 19.811360630003453 36.89236816212607 10-11 25.61792772352593 26.319683528089893 26.572941181227065 21.489447567157107 12-13 26.221732487438192 23.444572391332233 27.385806177494636 22.947888943734938 14-15 22.99007841592805 25.056488541762196 25.587108685332684 26.366324356977078 16-17 22.729544003985687 28.30463369017911 26.169586801220916 22.796235504614284 18-19 22.692334127355466 26.661023608589847 28.5427127182351 22.103929545819586 20-21 23.330449321454733 25.8417081779379 28.503256642385345 22.324585858222026 22-23 23.032185646074403 25.630883724212506 28.527845477730935 22.80908515198216 24-25 23.030636272301102 25.875573441176325 28.49217245787965 22.601617828642922 26-27 22.72400236188044 26.16025171338327 28.530696083950037 22.58504984078625 28-29 22.561377445238385 26.373051224535025 28.69974679747878 22.36582453274781 30-31 22.767589195173237 25.956878754253886 28.434525518175708 22.841006532397166 32-33 22.69804946950231 25.99499328677014 28.38362038072632 22.92333686300123 34-35 23.179176601482514 26.150946960856057 28.092743244172073 22.577133193489352 36-37 23.219325067389406 25.842241234322138 28.293497071444264 22.64493662684419 38-39 22.796457268944792 26.079223129532508 28.410915193270203 22.7134044082525 40-41 22.931100019042283 26.04903498265976 28.27861801547057 22.74124698282739 42-43 22.964799840917358 26.012235910591 27.934974602095824 23.08798964639582 44-45 22.955738190262434 26.205875147915187 27.888474110730883 22.949912551091497 46-47 23.05616236432257 26.547301098173275 27.565018484823366 22.83151805268078 48-49 23.255378026696345 26.290647384707217 27.572115305098194 22.881859283498247 50-51 23.09933632197334 26.599845557120894 27.451468291612564 22.849349829293207 52-53 23.348849295588543 26.597057094440807 27.085140052555428 22.96895355741522 54-55 23.05124291233253 26.44040405079928 27.270415073507387 23.237937963360796 56-57 23.071370302231394 26.692067783602774 27.298702764112715 22.937859150053118 58-59 23.21304031654722 26.305737997299623 27.36651889390776 23.114702792245396 60-61 23.196867263562147 26.495205165898266 27.54525187282557 22.762675697714016 62-63 22.91962928726194 26.605365153517198 27.47069233893421 23.004313220286654 64-65 22.78641174398276 26.354017178741202 27.39336428439768 23.466206792878353 66-67 22.884523992859314 26.491897262038698 27.31874738812539 23.304831356976596 68-69 22.792879878268412 26.421580158339275 27.411127119827693 23.374412843564617 70-71 22.944603622571965 26.445058369090983 27.290745389179037 23.319592619158016 72-73 23.029208522512384 26.393137619925707 27.230793601904185 23.346860255657727 74-75 22.91615133637931 26.80824420646242 26.872509645329025 23.40309481182925 76-77 22.98502739847388 26.530372052322605 27.02621710981326 23.45838343939026 78-79 22.978707685793644 26.627393138722567 27.101251972344357 23.29264720313943 80-81 22.879134434048083 26.81835339728461 27.06507144991559 23.237440718751714 82-83 23.047530696532974 26.683884977177225 26.947672826617104 23.3209114996727 84-85 22.874048928474423 26.617579657351452 26.935874596204645 23.572496817969476 86-87 22.883868488491814 26.505811993075167 26.99248558751911 23.61783393091391 88-89 22.81380249895399 26.499039551804366 26.977465748132623 23.70969220110902 90-91 22.747980822423013 26.67407339111193 27.050857458971052 23.527088327494006 92-93 22.908046944460473 26.440642955776482 27.005598094934957 23.645712004828084 94-95 22.707017594800252 26.372830696863765 27.043492099779947 23.876659608556032 96-97 22.688897571144985 26.344272363435483 27.198633316511973 23.76819674890756 98-99 22.652731033058203 26.43158294394885 26.783361334221816 24.132324688771135 100-101 22.587784465295385 25.966754271493368 27.356633240105037 24.08882802310621 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 977.0 1 642.5 2 447.0 3 619.5 4 512.5 5 469.0 6 913.5 7 2160.0 8 3534.0 9 3847.5 10 3095.0 11 2383.5 12 2320.5 13 2534.5 14 2883.5 15 3195.0 16 3414.5 17 3486.0 18 3658.0 19 3909.0 20 4232.0 21 4849.5 22 5668.5 23 7093.5 24 8778.5 25 10872.0 26 13545.0 27 16400.0 28 19133.5 29 22660.5 30 26857.0 31 31083.0 32 35523.0 33 39412.5 34 43195.5 35 47143.0 36 52744.5 37 58701.0 38 62524.0 39 64754.5 40 68079.5 41 74905.5 42 81079.0 43 86126.5 44 91385.5 45 96318.5 46 97946.0 47 101324.0 48 110998.5 49 112731.0 50 107347.0 51 104192.0 52 102394.5 53 102331.0 54 101811.0 55 95795.5 56 86035.5 57 78697.5 58 72608.0 59 63991.0 60 54032.0 61 44504.5 62 36445.5 63 29091.5 64 22367.5 65 16900.0 66 13023.0 67 11084.5 68 9028.0 69 6648.0 70 5017.5 71 3902.0 72 3148.5 73 2703.5 74 2237.5 75 1789.5 76 1247.0 77 701.0 78 423.5 79 280.0 80 159.0 81 88.0 82 60.0 83 30.5 84 18.5 85 13.5 86 7.0 87 3.5 88 2.5 89 4.5 90 3.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2659931261524868 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.002297163242300736 16-17 3.859234247065236E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 5.513191781521766E-5 26-27 0.0012312794978731943 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.00352844274017393 36-37 0.020196659226308068 38-39 0.007001753562532643 40-41 0.01782598676025371 42-43 0.0054580598637065485 44-45 5.696964840905825E-4 46-47 5.513191781521766E-5 48-49 0.0 50-51 0.0 52-53 1.8377305938405886E-5 54-55 0.0 56-57 1.8377305938405886E-5 58-59 5.696964840905825E-4 60-61 0.0021501447947934885 62-63 0.0015436936988260945 64-65 1.8377305938405886E-5 66-67 0.0 68-69 2.572822831376824E-4 70-71 1.8377305938405886E-5 72-73 0.0 74-75 0.0 76-77 0.2237804544119685 78-79 0.0 80-81 0.0011026383563043532 82-83 0.0 84-85 0.0 86-87 0.09936609320896062 88-89 0.3400720463902009 90-91 0.33986989602487844 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 2.389049771992765E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2720747.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.05575029182831 #Duplication Level Percentage of deduplicated Percentage of total 1 71.08753879569248 32.739899356350705 2 14.791529491735766 13.624699774111933 3 5.5250705015227854 7.633838020886401 4 2.7012137474146414 4.976257033431301 5 1.4591482788418546 3.360108438454576 6 0.9554515579538002 2.640242302143513 7 0.6142349108459206 1.9802334772110204 8 0.4454366729549665 1.6411936144349386 9 0.32270837661958623 1.3376318769605653 >10 1.8640384385777673 15.953561249935175 >50 0.15098847358393366 4.807570836919733 >100 0.07761103450717854 6.485907895085232 >500 0.003672493785291123 1.144669658099069 >1k 0.0011177154998636182 0.847976989333594 >5k 2.3951046425648963E-4 0.8262094766423491 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9448 0.3472575730121176 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7486 0.27514502450981293 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5536 0.20347353135002996 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3291 0.12095942768658756 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.8377305938405886E-5 0.0 12-13 0.0 0.0 0.0 3.675461187681177E-5 0.0 14-15 0.0 0.0 0.0 3.675461187681177E-5 0.0 16-17 0.0 0.0 0.0 3.675461187681177E-5 0.0 18-19 0.0 0.0 0.0 5.513191781521766E-5 0.0 20-21 0.0 0.0 0.0 9.188652969202942E-5 0.0 22-23 0.0 0.0 0.0 1.470184475072471E-4 0.0 24-25 0.0 0.0 0.0 3.3079150689130593E-4 0.0 26-27 0.0 0.0 0.0 3.8592342470652357E-4 0.0 28-29 0.0 0.0 0.0 0.001286411415688412 0.0 30-31 0.0 0.0 0.0 0.004079761918326107 0.0 32-33 0.0 0.0 0.0 0.009537821782032656 0.0 34-35 0.0 0.0 0.0 0.018211910184960232 0.0 36-37 0.0 0.0 0.0 0.034053147903866106 0.0 38-39 0.0 0.0 0.0 0.05673074343185897 0.0 40-41 0.0 0.0 0.0 0.08194440717935185 0.0 42-43 0.0 0.0 0.0 0.10951036608696067 0.0 44-45 0.0 0.0 0.0 0.1455482630321746 0.0 46-47 0.0 0.0 0.0 0.18915761002401177 0.0 48-49 0.0 0.0 0.0 0.23956656021305914 0.0 50-51 0.0 0.0 0.0 0.2928607474344362 0.0 52-53 0.0 0.0 0.0 0.3490769263000198 0.0 54-55 0.0 0.0 0.0 0.41367315667351645 0.0 56-57 0.0 0.0 0.0 0.4933020233046292 0.0 58-59 0.0 0.0 0.0 0.5841961784759847 0.0 60-61 0.0 0.0 0.0 0.6879728251101628 0.0 62-63 0.0 0.0 0.0 0.7925029412878155 0.0 64-65 0.0 0.0 0.0 0.9075448764622362 0.0 66-67 0.0 0.0 0.0 1.04526440716465 0.0 68-69 0.0 0.0 0.0 1.1997256635769515 0.0 70-71 0.0 0.0 0.0 1.374071165014608 0.0 72-73 0.0 0.0 0.0 1.5562821533939024 0.0 74-75 0.0 0.0 0.0 1.7522577439210627 0.0 76-77 0.0 0.0 0.0 1.9698634235377268 0.0 78-79 0.0 0.0 0.0 2.219482370099094 1.8377305938405886E-5 80-81 0.0 0.0 0.0 2.4978250458421893 3.675461187681177E-5 82-83 0.0 0.0 0.0 2.785576902225749 3.675461187681177E-5 84-85 0.0 0.0 0.0 3.084685933679243 3.675461187681177E-5 86-87 0.0 0.0 0.0 3.401106387326716 3.675461187681177E-5 88-89 0.0 0.0 0.0 3.7530501733531265 3.675461187681177E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8660 0.0 30.220415 1 GTATCAA 12290 0.0 29.579927 1 CGCTATA 135 4.1643034E-7 28.197582 1 ATCAACG 15330 0.0 23.526659 3 GTACATG 10340 0.0 23.42356 1 TCAACGC 15515 0.0 23.246126 4 CAACGCA 15610 0.0 23.135054 5 TATCAAC 15770 0.0 22.99061 2 TACATGG 10275 0.0 22.769522 2 AACGCAG 15985 0.0 22.59232 6 ACATGGG 9990 0.0 22.08901 3 GAGTACT 8925 0.0 21.588903 12-13 AGTACTT 9330 0.0 20.829796 12-13 AGAGTAC 14210 0.0 20.740074 10-11 GGACCGA 550 0.0 20.707964 6 TATACCG 115 0.0037447351 20.632933 5 ACGCAGA 17660 0.0 20.2614 7 GTACTTT 9465 0.0 20.256924 14-15 CATGGGG 6310 0.0 19.92991 4 CGCAGAG 18010 0.0 19.788599 8 >>END_MODULE