##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939241_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 254015 Sequences flagged as poor quality 0 Sequence length 101 %GC 34 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.083022656142354 33.0 31.0 34.0 30.0 34.0 2 32.27912918528433 34.0 31.0 34.0 30.0 34.0 3 32.30886364978446 34.0 31.0 34.0 30.0 34.0 4 35.92919315788438 37.0 35.0 37.0 35.0 37.0 5 35.88550282463634 37.0 35.0 37.0 35.0 37.0 6 35.95117611164695 37.0 35.0 37.0 35.0 37.0 7 35.89849024663898 37.0 35.0 37.0 35.0 37.0 8 35.90458831171387 37.0 35.0 37.0 35.0 37.0 9 37.49424640277149 39.0 37.0 39.0 34.0 39.0 10-11 37.451008798692996 39.0 37.0 39.0 34.0 39.0 12-13 37.377032852390606 39.0 37.0 39.0 34.0 39.0 14-15 38.48506190579296 40.0 38.0 41.0 34.0 41.0 16-17 38.45504989862803 40.0 38.0 41.0 33.5 41.0 18-19 38.5982048304234 40.0 38.0 41.0 34.0 41.0 20-21 38.62415014861327 40.0 38.0 41.0 34.0 41.0 22-23 38.51205046946046 40.0 38.0 41.0 34.0 41.0 24-25 38.40140149203788 40.0 38.0 41.0 34.0 41.0 26-27 38.14125346928331 40.0 37.0 41.0 33.5 41.0 28-29 37.88501663287602 40.0 37.0 41.0 33.0 41.0 30-31 37.5228116449816 39.5 36.0 41.0 33.0 41.0 32-33 37.15809893116548 39.0 35.0 41.0 33.0 41.0 34-35 36.938039092179594 39.0 35.0 41.0 32.0 41.0 36-37 36.66103970237978 39.0 35.0 41.0 31.0 41.0 38-39 36.389020333444876 39.0 35.0 41.0 31.0 41.0 40-41 36.14492844910734 39.0 35.0 41.0 30.5 41.0 42-43 35.82896088813653 39.0 35.0 41.0 30.0 41.0 44-45 35.40678503237998 38.0 35.0 41.0 28.0 41.0 46-47 34.8476645080015 38.0 35.0 40.5 24.5 41.0 48-49 34.349983268704605 38.0 34.5 40.0 22.5 41.0 50-51 34.06526189398264 38.0 34.0 40.0 20.0 41.0 52-53 33.79657500541306 38.0 34.0 40.0 19.5 41.0 54-55 33.54350530480484 38.0 33.0 40.0 15.5 41.0 56-57 33.21841426687401 38.0 33.0 40.0 10.0 41.0 58-59 32.89915753006712 37.5 33.0 40.0 6.0 41.0 60-61 32.55640611774895 37.0 33.0 40.0 2.0 41.0 62-63 32.16324035982127 36.0 32.0 39.5 2.0 41.0 64-65 31.839350038383557 36.0 32.0 39.0 2.0 40.5 66-67 31.388122748656574 35.5 31.0 39.0 2.0 40.0 68-69 30.859701198748105 35.0 31.0 38.5 2.0 40.0 70-71 30.372712241403065 35.0 30.5 37.5 2.0 40.0 72-73 29.836671062732513 35.0 29.5 37.0 2.0 39.0 74-75 29.271598133968467 34.5 29.0 36.5 2.0 39.0 76-77 27.338580398795347 32.5 26.0 35.0 2.0 37.0 78-79 28.213233470464342 34.0 28.0 35.0 2.0 37.0 80-81 28.157622581343624 34.0 28.0 35.0 2.0 37.0 82-83 27.778940220065742 34.0 27.0 35.0 2.0 37.0 84-85 27.324827273979885 34.0 26.0 35.0 2.0 36.0 86-87 26.71570773379525 33.0 25.5 35.0 2.0 36.0 88-89 26.069692734681023 33.0 23.5 35.0 2.0 35.0 90-91 25.517522587248784 32.5 20.5 35.0 2.0 35.0 92-93 25.154423951341457 32.0 19.0 35.0 2.0 35.0 94-95 24.609893116548236 32.0 12.0 34.0 2.0 35.0 96-97 23.857148199909453 31.5 2.0 34.0 2.0 35.0 98-99 22.910292699250043 31.0 2.0 34.0 2.0 35.0 100-101 18.979202015629 24.5 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.49275789702148387 1101 2 0.4450279018114429 1101 3 0.19230087030017273 1101 4 0.1199200115684107 1101 5 0.42225350366535963 1101 6 0.3062423155243863 1101 7 0.3625186330235053 1101 8 0.2339316594569283 1101 9 0.4933361716968818 1101 10-11 0.4415689440095605 1101 12-13 0.528874821565914 1101 14-15 0.2580551283575403 1101 16-17 0.21140569220971628 1101 18-19 0.4752230794245236 1101 20-21 0.46308287911066515 1101 22-23 0.5182916848020085 1101 24-25 0.5710090511690495 1101 26-27 0.7103477060821319 1101 28-29 0.5106687267413292 1101 30-31 0.8267247381984575 1101 32-33 0.9781746745738928 1101 34-35 0.8216620724416899 1101 36-37 0.7771700050281467 1101 38-39 0.8975610580267741 1101 40-41 0.28393586990944186 1101 42-43 1.0812301557878001 1101 44-45 1.135832553196856 1101 46-47 1.5180410342276858 1101 48-49 1.412247192073444 1101 50-51 1.4485837339633179 1101 52-53 1.3917021573846924 1101 54-55 1.349987780829764 1101 56-57 1.5284828094143492 1101 58-59 1.4275734839572536 1101 60-61 1.0512677301726185 1101 62-63 1.5047652732199097 1101 64-65 1.1128296576160004 1101 66-67 1.4443781113382173 1101 68-69 1.2448571440243228 1101 70-71 1.1367134124600469 1101 72-73 0.8556746293740964 1101 74-75 0.811729833492759 1101 76-77 0.6727567895410154 1101 78-79 1.1737232967752327 1101 80-81 1.4880826501674989 1101 82-83 1.0171771322587198 1101 84-85 0.9986265892235622 1101 86-87 0.4022947603922553 1101 88-89 0.7614630734866701 1101 90-91 0.8114346888480561 1101 92-93 0.27069848694094745 1101 94-95 0.5205222013332538 1101 96-97 0.4158927055354411 1101 98-99 0.3843247202334368 1101 100-101 0.8352510050458406 1102 1 0.25291463438891526 1102 2 0.2191428425475621 1102 3 0.16818592956405354 1102 4 0.12758142766323033 1102 5 0.2622995111513262 1102 6 0.029261654264253423 1102 7 0.29563232609898904 1102 8 0.287191172869278 1102 9 0.20988327088590353 1102 10-11 0.25137867576251693 1102 12-13 0.27060906985038 1102 14-15 0.3983974552446199 1102 16-17 0.3277890359402207 1102 18-19 0.4929242647021326 1102 20-21 0.7777760325729233 1102 22-23 0.5501655151201632 1102 24-25 0.501335002510281 1102 26-27 0.5235105257951744 1102 28-29 0.6981102426490082 1102 30-31 0.5763250969008951 1102 32-33 0.7800013277242357 1102 34-35 0.8524441997030792 1102 36-37 0.8497585109545369 1102 38-39 0.6822908615202223 1102 40-41 1.0073743446443117 1102 42-43 0.8296608326436967 1102 44-45 1.073966130863731 1102 46-47 0.8181034172595005 1102 48-49 0.5603581558912865 1102 50-51 0.6710962105696865 1102 52-53 0.9119668953759614 1102 54-55 1.1934258656706902 1102 56-57 0.906241309102434 1102 58-59 0.6765012755979356 1102 60-61 0.6299580763984451 1102 62-63 0.6668539351038731 1102 64-65 0.7842562795748229 1102 66-67 0.6420153540082083 1102 68-69 0.6912369787092913 1102 70-71 0.41041896454987636 1102 72-73 0.30689889637347534 1102 74-75 0.573299936424764 1102 76-77 0.3545156011442572 1102 78-79 0.0414712693266992 1102 80-81 0.24532493026731217 1102 82-83 0.4680992314477379 1102 84-85 0.264641483172408 1102 86-87 0.4854006555887622 1102 88-89 0.8179099699308381 1102 90-91 1.1301827393783128 1102 92-93 0.8729384276770666 1102 94-95 1.2635918363925178 1102 96-97 1.0490999731586506 1102 98-99 0.6956452910381792 1102 100-101 0.7804319158381077 1103 1 0.24430010481368925 1103 2 0.37221946025299957 1103 3 0.3878365845858909 1103 4 0.3480855959839886 1103 5 0.3009061010679588 1103 6 0.31598257526464835 1103 7 0.36276213951701664 1103 8 0.2004901010153688 1103 9 0.3248296781903903 1103 10-11 0.4490364764770902 1103 12-13 0.5253033929944806 1103 14-15 0.6970973361497457 1103 16-17 0.6803991987032205 1103 18-19 0.7609373651388083 1103 20-21 0.5760293076820986 1103 22-23 0.8212949315552578 1103 24-25 0.7299376225976246 1103 26-27 0.7895684853029152 1103 28-29 0.7750356098582145 1103 30-31 0.9493302576789802 1103 32-33 1.0518353888596081 1103 34-35 0.7973925919222111 1103 36-37 1.0926326673658053 1103 38-39 1.05636787620859 1103 40-41 1.2219634673120368 1103 42-43 1.4434460648787066 1103 44-45 1.4432595012488107 1103 46-47 1.3790799952666433 1103 48-49 1.409365698566944 1103 50-51 0.9692006170802046 1103 52-53 0.8130089755665111 1103 54-55 1.0044520665440615 1103 56-57 1.213913004219556 1103 58-59 1.3398299774637579 1103 60-61 1.3371037691336625 1103 62-63 1.5943350784147157 1103 64-65 1.28007803423937 1103 66-67 1.1029820074421082 1103 68-69 0.9617808453230268 1103 70-71 0.7610640618106927 1103 72-73 1.221746057945527 1103 74-75 0.7461860023239275 1103 76-77 0.7450376336968567 1103 78-79 1.037034985086919 1103 80-81 0.9947790787389295 1103 82-83 0.7966820023885894 1103 84-85 0.9389269138988858 1103 86-87 0.8789993058468006 1103 88-89 1.189912748811345 1103 90-91 0.6919135849519513 1103 92-93 0.5987423181097746 1103 94-95 0.6144751234111716 1103 96-97 0.5236037444964801 1103 98-99 0.2723523176360345 1103 100-101 0.3699912647861012 1104 1 0.06880603366744253 1104 2 0.2038400136126839 1104 3 0.09218441067284289 1104 4 0.09689982523300245 1104 5 0.18331716826163813 1104 6 0.2813975950274923 1104 7 0.1686909932719587 1104 8 0.3601738954817719 1104 9 0.41988896672793885 1104 10-11 0.12650683220830672 1104 12-13 0.3307381756031802 1104 14-15 0.6400815258730645 1104 16-17 0.26784979158859556 1104 18-19 0.20985040861707205 1104 20-21 -0.06310112710051641 1104 22-23 0.11289572206909071 1104 24-25 0.30167675303240316 1104 26-27 0.02059615328710862 1104 28-29 0.42809885096492906 1104 30-31 0.3671168189438063 1104 32-33 0.30183538885960814 1104 34-35 0.26191828362181724 1104 36-37 0.4582453155871562 1104 38-39 0.6664469275919913 1104 40-41 0.4652440997231082 1104 42-43 0.10935515578780297 1104 44-45 0.01974171468754804 1104 46-47 -0.10214530117604426 1104 48-49 -0.03253153463463576 1104 50-51 -0.20599701137829385 1104 52-53 -0.2910424078326983 1104 54-55 -0.22054793345594703 1104 56-57 -0.15061268748005574 1104 58-59 -0.14109887629118845 1104 60-61 0.17050297861982955 1104 62-63 0.03494772663606227 1104 64-65 0.0663428563737618 1104 66-67 0.2874681734500193 1104 68-69 0.26079270303053903 1104 70-71 0.13112335034824696 1104 72-73 0.18044171011944243 1104 74-75 0.1372927216915194 1104 76-77 0.5081799261869762 1104 78-79 0.7817978309367248 1104 80-81 0.7154312526519746 1104 82-83 0.6498440577245574 1104 84-85 0.5951522103415741 1104 86-87 0.6607186734357349 1104 88-89 0.5554265828034417 1104 90-91 0.41009540313377 1104 92-93 0.20980951178566798 1104 94-95 0.18137235661275497 1104 96-97 0.20156817137394967 1104 98-99 -0.24632357169202734 1104 100-101 -0.055898063277140864 1105 1 0.15266220684667786 1105 2 0.14674957149197354 1105 3 0.17728806137913722 1105 4 0.2860027421289857 1105 5 0.10901887437060509 1105 6 0.34120313529533064 1105 7 0.17507514296925564 1105 8 0.15338615011395262 1105 9 0.29178403185360224 1105 10-11 0.3662976962699531 1105 12-13 0.17836246472443662 1105 14-15 0.34649319345699325 1105 16-17 0.46159405869554604 1105 18-19 0.5631525550122305 1105 20-21 0.6249744553614178 1105 22-23 0.6468894846814521 1105 24-25 0.4850009137368616 1105 26-27 0.3953989417662811 1105 28-29 0.5068825603760558 1105 30-31 0.37825239039857905 1105 32-33 0.27862863780475067 1105 34-35 0.4703020664139643 1105 36-37 0.4050991038828755 1105 38-39 0.24071770359639544 1105 40-41 0.20982307605771666 1105 42-43 0.1530260418637468 1105 44-45 0.1635318449388734 1105 46-47 0.042754717169209755 1105 48-49 -0.002582901356340983 1105 50-51 -0.4654081286044942 1105 52-53 -0.15570026877564658 1105 54-55 0.30434658131198944 1105 56-57 0.3783453018796976 1105 58-59 0.2803650752773308 1105 60-61 0.2675218742353067 1105 62-63 0.38327830818073494 1105 64-65 0.27387358468049783 1105 66-67 0.324011926889753 1105 68-69 0.30508732787001236 1105 70-71 0.1893053353051357 1105 72-73 0.26604985541387904 1105 74-75 0.4203325309008399 1105 76-77 0.5264242850202159 1105 78-79 0.8207998489150761 1105 80-81 0.6250533403423049 1105 82-83 0.7546413426264102 1105 84-85 1.1766235000131964 1105 86-87 1.3507388532192586 1105 88-89 1.1598110994058999 1105 90-91 1.5818253609396784 1105 92-93 1.6613046502923616 1105 94-95 1.6948836389462691 1105 96-97 1.8150977989652155 1105 98-99 1.2601064411496488 1105 100-101 0.9793890400066587 1106 1 0.19248931397850555 1106 2 0.24827267533060393 1106 3 0.10653577157776084 1106 4 0.16837384432101743 1106 5 0.10449072782331825 1106 6 0.2319101436312394 1106 7 0.22107699539284908 1106 8 -0.13816474007849422 1106 9 0.18192502187110193 1106 10-11 0.15483839813266798 1106 12-13 0.14298631982375554 1106 14-15 0.10565239897310619 1106 16-17 0.3344457618961272 1106 18-19 0.3960999667648224 1106 20-21 0.5061106084951064 1106 22-23 0.4903082353246617 1106 24-25 0.6185555087764811 1106 26-27 0.6987333042238291 1106 28-29 0.3332950333615372 1106 30-31 0.08240490660728739 1106 32-33 0.2428922994287106 1106 34-35 0.05180870426514872 1106 36-37 -0.2429918706977574 1106 38-39 -0.02075703140412344 1106 40-41 0.06335297211617785 1106 42-43 -0.07641720193577584 1106 44-45 0.0373097599332155 1106 46-47 -0.21308556275257473 1106 48-49 -0.4465470878336433 1106 50-51 -0.27579199193679926 1106 52-53 -0.5159452003388836 1106 54-55 -0.37217394971610673 1106 56-57 0.08998469675465515 1106 58-59 0.15488910532775435 1106 60-61 -0.08129977853817394 1106 62-63 -0.2203545002992513 1106 64-65 -0.2504652030111778 1106 66-67 -0.19042154022972824 1106 68-69 -0.22151553309530314 1106 70-71 -0.26862921239477444 1106 72-73 0.02459158640081327 1106 74-75 0.08838851525814917 1106 76-77 0.6383672715386908 1106 78-79 0.86332840711205 1106 80-81 0.5588034689828305 1106 82-83 0.6596531775548868 1106 84-85 0.4955233662270473 1106 86-87 0.773991914863803 1106 88-89 0.9934788981092026 1106 90-91 1.229607598255722 1106 92-93 1.3370276100536067 1106 94-95 1.2779082350150155 1106 96-97 1.2245091399140726 1106 98-99 1.339554830570254 1106 100-101 1.306650540469768 1107 1 0.1087238700248534 1107 2 0.26248431350997947 1107 3 0.24295469482211018 1107 4 0.11411995532543528 1107 5 0.06092757565636475 1107 6 0.028402045558131306 1107 7 0.1503068782428585 1107 8 0.09683942098673981 1107 9 0.35765000746741293 1107 10-11 0.27888615435826125 1107 12-13 0.04715378669526871 1107 14-15 0.33612740057350976 1107 16-17 0.08310141774402524 1107 18-19 0.3229452391545564 1107 20-21 0.2480765517765846 1107 22-23 0.3383493338458834 1107 24-25 0.22757541787320434 1107 26-27 0.1661969749235297 1107 28-29 0.3041515726355897 1107 30-31 0.21826368645492522 1107 32-33 0.3136464124816527 1107 34-35 0.4606173592808389 1107 36-37 0.14227475755907193 1107 38-39 0.7566899950346482 1107 40-41 0.5339534458374402 1107 42-43 0.6475441321657556 1107 44-45 0.5295157646704283 1107 46-47 0.8065674684234097 1107 48-49 0.7488789412297976 1107 50-51 0.6660331152906522 1107 52-53 0.4284885747075293 1107 54-55 0.4499245074889444 1107 56-57 -0.04419365290285526 1107 58-59 0.14027736472215935 1107 60-61 0.49859267392965734 1107 62-63 -0.09283385501406372 1107 64-65 -0.4126027102130223 1107 66-67 -0.39596215862803774 1107 68-69 0.2953527851941793 1107 70-71 -0.14843844355795 1107 72-73 0.0859192862919862 1107 74-75 0.32116810683359276 1107 76-77 0.16778280191446626 1107 78-79 0.4701237467189898 1107 80-81 0.27332238582554425 1107 82-83 0.19854313338358054 1107 84-85 0.577384322631886 1107 86-87 0.18846602023477388 1107 88-89 0.6494868361413388 1107 90-91 0.7463158755747195 1107 92-93 0.7883271427339729 1107 94-95 0.4338201484648607 1107 96-97 1.0774154839381431 1107 98-99 0.8872950520669569 1107 100-101 0.34973356972525593 1108 1 -0.11190480139121561 1108 2 0.10028583831937965 1108 3 0.1799000766493819 1108 4 0.15017794807634743 1108 5 0.13207493223679023 1108 6 0.0205280298101016 1108 7 -0.027858351103475343 1108 8 0.005757056282327255 1108 9 -0.04833049496978248 1108 10-11 0.11220386464447074 1108 12-13 0.16577958347067323 1108 14-15 0.42224163629404643 1108 16-17 0.07549299379701324 1108 18-19 0.322445301646745 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-0.8855318991244054 2316 100-101 -0.9207401536326145 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 9.0 5 22.0 6 89.0 7 134.0 8 222.0 9 428.0 10 577.0 11 912.0 12 1211.0 13 1625.0 14 2075.0 15 2664.0 16 3581.0 17 4707.0 18 4228.0 19 2689.0 20 2360.0 21 2539.0 22 2523.0 23 2782.0 24 3142.0 25 3373.0 26 3566.0 27 4055.0 28 4866.0 29 5730.0 30 6834.0 31 7885.0 32 9546.0 33 11879.0 34 16704.0 35 26279.0 36 29910.0 37 48517.0 38 34708.0 39 1642.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.87720856776231 25.572204125950055 16.84216760438259 17.708419701905044 2 14.47040529102612 26.055154223175798 39.54294037753676 19.931500108261325 3 17.37928862468752 31.277286774403084 34.28301478259158 17.060409818317815 4 10.691494596775781 23.11595771903234 39.66025628407771 26.532291400114165 5 9.073873590142314 42.82817943822215 35.76363600574769 12.334310965887841 6 21.916815936066765 43.63600574769207 18.882743145089854 15.564435171151311 7 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37.65230399779541 15.650256874594021 30-31 15.9484676101805 31.986693699190994 36.64665472511466 15.41818396551385 32-33 15.362872271322559 32.77188355018404 36.359270121843196 15.5059740566502 34-35 15.714052316528976 31.89757156664167 36.3788647364391 16.009511380390258 36-37 16.661646912457456 31.349054022397947 35.80423352290288 16.185065542241723 38-39 15.861777095389945 32.091846777685916 36.13479784260462 15.911578284319514 40-41 15.06209437873142 32.18868659907051 37.24855664278952 15.500662379408563 42-43 15.228825069385666 32.44198177272995 36.015786469302995 16.313406688581384 44-45 15.574294864602587 32.6227355848062 35.21927156841324 16.583697982177974 46-47 16.215216060500484 32.50090053914245 33.986642494818206 17.297240905538857 48-49 16.210098242423168 32.05387881400471 34.45295445732429 17.28306848624783 50-51 16.147274767238155 32.45635100289353 34.15310119481133 17.243273035056987 52-53 15.79926382300258 33.25492589020334 34.57158041847922 16.374229868314863 54-55 15.726827155876622 32.48941991614668 34.1428655788044 17.640887349172292 56-57 16.472876228044793 33.51797064306143 32.754816218950715 17.254336909943056 58-59 15.922829657945863 32.452526381887296 33.81625490385202 17.808389056314823 60-61 16.152322912606834 32.90441827780468 33.47092471645152 17.472334093136965 62-63 16.590981116996673 32.53199166179484 33.56206313493681 17.314964086271683 64-65 16.23467118083578 32.51107218077672 33.85311891030057 17.401137728086923 66-67 15.734399943310098 32.80534302310704 33.108673357912075 18.35158367567078 68-69 15.536365622680954 32.33193116032605 33.52774786869047 18.60395534830253 70-71 16.00614136960416 32.78270968249907 33.130917465504005 18.080231482392772 72-73 16.387024388323525 31.32354388520363 33.02659291774108 19.262838808731768 74-75 15.884298171367833 32.50556069523453 32.57504478082003 19.035096352577604 76-77 16.24027245031649 31.272197755362978 33.466322552130194 19.021207242190336 78-79 15.630966675196348 31.436135661279845 33.57144263134067 19.361455032183137 80-81 15.94101911505052 31.605479214769232 32.9928015920351 19.460700078145145 82-83 15.894730626144124 31.684152510678505 32.6321280239356 19.788988839241778 84-85 15.651241068440841 30.594059405940595 32.7825128437297 20.972186681888864 86-87 16.029302528111472 30.815533177153014 32.83569738322434 20.31946691151117 88-89 16.93028161503 29.74635290957428 32.85482348200848 20.46854199338724 90-91 15.978838275804911 29.559716159790355 33.70010497983282 20.76134058457191 92-93 16.189004586343327 30.421825482747085 33.12914591657973 20.260024014329865 94-95 16.150227348778614 29.49747062181367 32.9903745841781 21.36192744522961 96-97 16.400803102179005 29.048875066433084 32.15912446115387 22.391197370234043 98-99 16.177816620704725 29.37430737221694 31.938727907265136 22.5091480998132 100-101 16.41284257394755 28.577502465410436 33.37919685687235 21.63045810376967 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4344.0 1 2619.0 2 1018.5 3 1038.5 4 791.5 5 817.0 6 1418.0 7 2691.0 8 3616.5 9 3574.5 10 2635.5 11 1613.5 12 1385.5 13 1300.5 14 1203.5 15 1117.0 16 992.0 17 894.0 18 861.0 19 898.0 20 971.0 21 1010.5 22 1082.5 23 1217.0 24 1324.0 25 1692.5 26 2629.0 27 3669.5 28 4040.5 29 4343.0 30 5408.5 31 6428.5 32 7680.0 33 9405.0 34 11018.5 35 11844.0 36 12488.0 37 13529.5 38 12485.5 39 10504.5 40 9364.0 41 8365.5 42 7443.0 43 7167.0 44 7110.0 45 6552.0 46 5906.0 47 5946.0 48 10060.5 49 9789.0 50 4814.5 51 3257.0 52 2485.5 53 1906.0 54 1390.5 55 1121.5 56 969.5 57 818.5 58 720.0 59 625.5 60 514.0 61 460.5 62 417.5 63 334.0 64 222.0 65 156.0 66 115.5 67 98.0 68 83.0 69 65.0 70 57.0 71 44.5 72 47.5 73 51.5 74 37.5 75 23.0 76 16.5 77 12.0 78 6.0 79 4.0 80 2.5 81 2.0 82 2.0 83 0.5 84 1.5 85 1.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29132137865874064 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 7.873550774560558E-4 16-17 5.905163080920418E-4 18-19 0.0 20-21 1.9683876936401395E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0013778713855480975 36-37 0.007283034466468516 38-39 0.0019683876936401395 40-41 0.002558904001732181 42-43 0.0 44-45 0.0013778713855480975 46-47 1.9683876936401395E-4 48-49 1.9683876936401395E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0013778713855480975 58-59 0.0021652264630041534 60-61 7.873550774560558E-4 62-63 0.0013778713855480975 64-65 0.0 66-67 5.905163080920418E-4 68-69 0.0017715489242761256 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.24053697616282502 78-79 0.0 80-81 1.9683876936401395E-4 82-83 0.0 84-85 0.0 86-87 0.09782886837391491 88-89 0.3417121036159282 90-91 0.24919788201484164 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.9683876936401395E-4 100-101 1.9683876936401395E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 254015.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.913883038403245 #Duplication Level Percentage of deduplicated Percentage of total 1 69.10168286323774 17.215912446115386 2 11.053172157699297 5.50754876680511 3 5.026467567354033 3.75686475208157 4 2.9754286165758077 2.965179221699506 5 2.016275578731137 2.5116626970848177 6 1.3257485976139687 1.9817727299568921 7 0.9733744173184798 1.697537546995256 8 0.8011377103579046 1.596756097080881 9 0.6573437623449475 1.4739287049977363 >10 5.175001975191593 25.693364565084735 >50 0.5735956387769614 9.893903903312797 >100 0.28916804930078216 13.047654666063027 >500 0.018961839298411946 3.262799440977895 >1k 0.011061072924073635 7.23421845166624 >5k 0.0015801532748676622 2.1608960100781447 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5489 2.1608960100781447 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4807 1.89240792866563 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4152 1.6345491407987716 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3295 1.297167490108852 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1912 0.7527114540479893 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1580 0.622010511190284 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1411 0.5554790071452473 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1219 0.47989291970946596 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 991 0.3901344408794756 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 927 0.36493907840088186 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 820 0.3228155817569829 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 728 0.2865972481940043 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 716 0.281873117729268 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 647 0.25470936755703405 No Hit CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT 627 0.2468358167824735 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 612 0.24093065370155306 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 586 0.23069503769462432 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 577 0.22715193984607207 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 543 0.21376690352931912 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 514 0.2023502549062063 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 440 0.17321811704033227 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 434 0.1708560518079641 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 426 0.16770663149813986 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 404 0.15904572564612326 No Hit ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT 395 0.155502627797571 TruSeq Adapter, Index 10 (95% over 21bp) CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 366 0.1440859791744582 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 360 0.14172391394209005 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 359 0.141330236403362 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 354 0.13936184870972185 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 349 0.13739346101608174 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 347 0.13660610593862568 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 340 0.13385036316752946 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 313 0.12322106962187272 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 308 0.12125268192823258 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 305 0.12007164931204851 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 303 0.11928429423459246 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 301 0.1184969391571364 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 300 0.11810326161840837 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 297 0.11692222900222426 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 291 0.1145601637698561 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 286 0.11259177607621598 No Hit CGTCTATGTGGCAAAATAGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCC 283 0.11141074346003188 No Hit GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT 282 0.11101706592130386 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 280 0.11022971084384779 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 275 0.10826132315020767 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 272 0.10708029053402357 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 266 0.10471822530165541 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 262 0.1031435151467433 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 256 0.10078144991437514 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 3.936775387280279E-4 0.0 20-21 0.0 0.0 0.0 9.841938468200698E-4 0.0 22-23 0.0 0.0 0.0 0.00551148554219239 0.0 24-25 0.0 0.0 0.0 0.01200716493120485 0.0 26-27 0.0 0.0 0.0 0.019683876936401393 0.0 28-29 0.0 0.0 0.0 0.036218333562978565 0.0 30-31 0.0 0.0 0.0 0.07637344251323741 0.0 32-33 0.0 0.0 0.0 0.14487333425191426 0.0 34-35 0.0 0.0 0.0 0.24762317185992952 0.0 36-37 0.0 0.0 0.0 0.4048973485817767 0.0 38-39 0.0 0.0 0.0 0.5777217880833809 0.0 40-41 0.0 0.0 0.0 0.7357833198826841 0.0 42-43 0.0 0.0 0.0 0.8881365273704309 0.0 44-45 0.0 0.0 0.0 1.0857626518119008 0.0 46-47 0.0 0.0 0.0 1.3310237584394624 0.0 48-49 0.0 0.0 0.0 1.5770722201444796 0.0 50-51 0.0 0.0 0.0 1.8258764246205934 0.0 52-53 0.0 0.0 0.0 2.0587366887782217 0.0 54-55 0.0 0.0 0.0 2.3223038009566364 0.0 56-57 0.0 0.0 0.0 2.655551837489912 0.0 58-59 0.0 0.0 0.0 2.990571422947464 0.0 60-61 0.0 0.0 0.0 3.378146959825207 0.0 62-63 0.0 0.0 0.0 3.740527134224357 0.0 64-65 0.0 0.0 0.0 4.1401098360333055 0.0 66-67 0.0 0.0 0.0 4.576698226482687 0.0 68-69 0.0 0.0 0.0 5.065055213274807 0.0 70-71 0.0 0.0 0.0 5.620927897958782 0.0 72-73 0.0 0.0 0.0 6.194712910654882 0.0 74-75 0.0 0.0 0.0 6.806290967068874 0.0 76-77 0.0 0.0 0.0 7.415506958250496 0.0 78-79 0.0 0.0 0.0 8.108969942719918 0.0 80-81 0.0 0.0 0.0 8.838060744444226 0.0 82-83 0.0 0.0 0.0 9.593331102493948 0.0 84-85 0.0 0.0 0.0 10.411589866740155 0.0 86-87 0.0 0.0 0.0 11.279648839635454 0.0 88-89 0.0 0.0 0.0 12.120544062358523 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTTAAG 15 6.578746E-4 95.120316 1 CTTAAGG 20 0.0020780126 71.1998 2 GTCTAGA 25 0.0049917046 57.072193 1 AGGGTCT 55 8.205079E-7 51.781677 5 TAGGGTC 55 8.205079E-7 51.781677 4 CATATAG 70 7.810013E-8 47.560158 1 TAGGCAT 110 0.0 47.466537 5 CTTTAGG 115 1.8189894E-12 45.40277 2 GGATATG 65 2.5939225E-6 43.81526 7 CCTTTAG 120 1.8189894E-12 43.59681 1 CATGGGT 165 0.0 43.151394 4 CATTCCG 135 0.0 42.19248 9 TTAGGCA 130 5.456968E-12 40.16399 4 ATATGAG 75 6.934484E-6 37.97323 9 GGCATTC 150 0.0 37.97323 7 TGGGATA 50 0.0016190422 37.97323 8 GTGGTAC 65 1.3154342E-4 36.58474 1 TGGGTAT 65 1.3307793E-4 36.51272 6 CCCTATA 70 2.0573467E-4 33.90467 2 AGGCATT 155 3.6379788E-11 33.68593 6 >>END_MODULE